scholarly journals Fractionation of Garcinia Mangostana Fruit Pericarp Cellulase Assisted Extracts by Preparative Thin Layer Chromatography and High Performance Liquid Chromatography

2020 ◽  
Vol 18 (1) ◽  
pp. 31
Author(s):  
Titania Tjandrawati Nugroho ◽  
Krisna Puja ◽  
Yum Eryanti ◽  
Miranti Miranti
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Preparative Thin Layer Chromatography ◽  
Garcinia Mangostana ◽  
Fruit Pericarp ◽  
Layer Chromatography

The polar extract of Mangosteen (Garcinia mangostana) fruit pericarp obtained by cellulase assisted ethanol extraction has strong antioxidant activity, giving an average 2,2 diphenyl-1 pykrylhydrazyl (DPPH) radical  scavenging IC50 of 13.9 µg/mL. In order to elucidate the chemical component from this extract that is responsible for the high antioxidant activity, fractionation of the extract should firstly be performed. In this paper we show results of preparative fractionation of the polar extract by two methods, namely preparative Thin Layer Chromatography (PTLC) and preparative High Performance Liquid Chromatography (PHPLC). PTLC used Silica Gel G60 plates, with a hexane:ethyl acetate (6:4) eluent. PHPLC was a reverse phase method, using C18 column and water:acetonitrile gradient elution. 4 fractions from PTLC and 6 fractions from PHPLC were collected and their antioxidant activity analyzed. Both methods gave separated fractions with lower antioxidant activity than the unfractionated original crude extracts, showing that the strong antioxidant activity of Mangosteen pericarp polar extracts maybe due to the concerted synergetic effect of several compounds, rather than a single isolated compound. It also shows the high degree of difficulty in separating mangosteen pericarp polar components having antioxidant activity for further structural analysis.

Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography

2017 ◽  
Vol 54 (3) ◽  
pp. 294
Author(s):  
Hari Ramakrishnan K. ◽  
Janaky Ranjithkumar
Keyword(s):  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Highly Sensitive ◽  
Layer Chromatography

Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

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