scholarly journals Identification of Rice Genotypes Resistant To Bacterial Leaf Blight Disease Using Ssr Markers

2018 ◽  
Vol 35 (1) ◽  
pp. 113-120
Author(s):  
A. Acharya ◽  
N. R. Adhikari ◽  
R. B. Amgain ◽  
A. Poudel ◽  
R. Yadav ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Bacterial Blight ◽  
Gene Pyramiding ◽  
Bacterial Pathogen ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Resistant Varieties ◽  
Blight Disease ◽  
Almost All

 Bacterial blight disease of rice is a growing and challenging concern in Nepal. Since bacterial pathogen (Xanthomonas oryzae pv. oryzae) is difficult to manage by other means effectively, development of host plant resistance is the most effective mean to control this disease. This study was carried out to identify the bacterial leaf blight resistant genotypes of rice by using of molecular markers linked with bacterial blight resistant genes. Sixty genotypes of rice were screened at glass house for BB resistance and they were tested for the presence of Xa4, Xa5, Xa7 and Xa21 genes using markers MP, RM122, M5 and pTA248 respectively. IRBB 60 and Jumli Marshi were used as resistant and susceptible check respectively. Twenty five genotypes of rice were detected with presence of Xa4 gene, 24 genotypes with Xa5 gene and fourteen genotypes with Xa7 gene. Twenty four genotypes did not showed presence of any gene. Twenty four genotypes showed the presence of more than one gene with the specific molecular markers. Almost all genotypes that did not show presence of any gene were found highly susceptible in greenhouse conditions with both inoculums. Genotypes having more than one BB resistance gene were found resistant in greenhouse conditions with both inoculums suggesting combination of BB resistance gene through gene pyramiding will provide BB resistant varieties in rice breeding.

scholarly journals IDENTIFIKASI MARKA GEN KETAHANAN HAWAR DAUN BAKTERI PADA GALUR PADI INTRODUKSI DAN GALUR DIHAPLOID

2014 ◽  
Vol 14 (2) ◽  
pp. 101-109
Author(s):  
Ovi Prasetya Winandari ◽  
Aris Tjahjoleksono . ◽  
Dwinita Wikan Utami
Keyword(s):  
Molecular Markers ◽  
Selection Process ◽  
Control Plant ◽  
Bacterial Pathogen ◽  
Leaf Blight ◽  
Breeding Program ◽  
Bacterial Leaf Blight ◽  
Rice Varieties ◽  
Rice Germplasm ◽  
Resistant Varieties

Identification marker of bacterial leaf blight (BLB) gene resistance on introducted and dihaploid rice germplasm. Bacterial leaf blight (BLB), caused by bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating diseases in rice. The use of BLB-resistant rice varieties is one of the most efficient ways to protect rice from this disease. BLB-resistant varieties can be produced through the breeding program by using the diverse  rice germplasm. The objective of this research was to identify BLB resistance gene on 37 introducted and dihaploid rice lines derived from wide genetic background double crossing from local rice : IR54/Parekaligolara and Bio110/Markuti, by using the molekular markers. As control plant  used 23 differential varieties (monogenic lines/IRBB) and TN1 (susceptible).  All plants tested were inoculated by 3 selected dominant BLB races (Race III, IV dan VIII). The selected of 19 polymorphism molecular markers used to identify the BLB resistance genes on rice lines tested. The result of this research showed that 4 rice lines were resistance to all BLB races tested. Three molecular markers were specific associated with resistant gene to Race III (Xa7-STS40, Xa1-STS14 and Xa4-STS50); Race IV (Xa1-STS5, Xa4-STS50 and Xa26-STS1), and Race VIII (Xa21-STS6, Xa7-RM20590 and Xa7-STS40). These markers could be utilized for the selection process the developmen of BLB resistance rice lines breeding program. 

scholarly journals Marker-assisted selection and gene pyramiding for resistance to bacterial leaf blight disease of rice (Oryza sativa L.)

2019 ◽  
Vol 33 (1) ◽  
pp. 440-455 ◽  
Author(s):  
Samuel Chibuike Chukwu ◽  
Mohd Y. Rafii ◽  
Shairul Izan Ramlee ◽  
Siti Izera Ismail ◽  
Yussuf Oladosu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Marker Assisted Selection ◽  
Oryza Sativa L ◽  
Gene Pyramiding ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Blight Disease

scholarly journals Stacking of Pup1 QTL for Low Soil Phosphorus Tolerance and Bacterial Blight Resistance Genes in the Background of APMS6B, the Maintainer Line of Rice Hybrid DRRH-3

2021 ◽  
Author(s):  
Madhusudan N ◽  
Beulah P ◽  
VEERENDRA JALDHANI ◽  
Nagaraju P ◽  
Manasa Y ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Soil Phosphorus ◽  
Female Parent ◽  
Field Evaluation ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
P Deficiency ◽  
Foreground Selection ◽  
Blight Disease ◽  
Marker Assisted Backcross Breeding

Abstract Phosphorus (P) is one of the macro nutrients essential for plant growth and development. Rice (Oryza sativa L.) is sensitive to P starvation and its deficiency influences many key plant functions which results in crop yield penalty. Although the hybrid rice segment is well-known for its yield heterosis, P deficiency and bacterial leaf blight (BLB) diseases are the evident limitations. APMS6B, the female parent of DRRH-3 is susceptible to low P and bacterial blight disease. In the present study, the improvement of APMS6B to P starvation and resistance to bacterial leaf blight (BB) was carried out using marker-assisted backcross breeding (MABB) approach. Kasalath (+ Pup1 QTL) was used as donor and a promising IL (ATR 594-1) at BC1 F4 generation was identified with 81.15% RPGR. Concurrently, this IL was intercrossed with GU-2 (+ Xa21 and Xa38 ). Hybridity of Intercross F1s (ICF1) was confirmed through foreground selection having maximum RPGR (88.29%) and were selfed to produce ICF2. The resultant progenies were phenotyped for BB using Xoo inoculum (IX-020), simultaneously genotyped with gene specific functional SSR markers for Xa21 and Xa38. The identified BB resistant plants were subjected to foreground selection for Pup1. Four promising ICF3 plants (BP-10-1, BP-10-3, BP-10-5 and BP-10-15 with Xa21, Xa38 and Pup1) along with parents and checks were screened both in low P plot (<2 kg P2O5 ha-1 ) as well as in normal plot (>25 kg P2O5 ha-1) during dry and wet seasons 2018. Based on the field evaluation, four promising intercrossed lines were identified with better root architecture in terms of root length and root volume. In addition, less % reduction in grain yield (39.10%) under P starvation and less susceptibility indices values (<1) for BB were observed. These lines may be utilized in the CMS conversion programme and development of climate resilient, biotic and abiotic tolerant rice hybrids.

scholarly journals Evaluation of Local Black Glutinous Rice Germplasm of Vietnam for Resistance to Bacterial Leaf Blight Disease

2019 ◽  
Vol 1 (3) ◽  
pp. 240-248
Author(s):  
Tung Hoang ◽  
Ton Huu Phan ◽  
Hai Van Tong ◽  
Trung Nam Tran
Keyword(s):  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Resistant Genes ◽  
Glutinous Rice ◽  
Resistant Varieties ◽  
Environmentally Safe ◽  
Susceptible Control ◽  
Blight Disease ◽  
North Vietnam

Most rice growing areas frequently encounter the bacterial leaf blight, Xanthomonas oryzae pv oryzae (Xoo). To prevent the disease, development of resistant varieties is considered to be the most economical and environmentally safe solution. In this study, three PCR-based markers, Npb181, RM122, and P3, were used for the identification of the genes Xa4, xa5, and Xa7, respectively, from 56 local black glutinous rice accessions of Vietnam. Phenotypic screening of the accessions for resistance to 10 Xoo strains of North Vietnam, along with IRBB4, IRBB5, and IRBB7 as resistant controls and IR24 as a susceptible control were carried out in the 2016 Autumn season. 19 accessions containing the resistant genes were found, of these, 6 accessions carried Xa4 gene, 6 accessions carried xa5 gene, and 11 accessions carried Xa7 gene. Three accessions carried two resistance genes, viz. Nep do (Xa4 and Xa7), Pau cam (xa5 and Xa7), and Pe lon cam (Xa4 and xa5). Accessions with xa5 and Xa7 alone or with a combination of two genes (Xa4 and xa5, Xa4 and Xa7, or xa5 and Xa7) were resistantto 8-9 Xoo strains (8-9R/0M/1-2S). Accessions containing Xa4 showed resistance to 5-6 strains of Xoo (5-6R/0M/4-5S). Xoo strain No1 (HUA01043) showed the lowest virulence, infecting only 14 accessions (42R/4M/14S). Strains No3 (HUA 0020131-2), No4 (HUA202361), No5 (HUA20212), and No8 (HUA 020083) showed highest virulence, and they each infected more than 40 accessions with 19R/0M/41S, 20R/0M/40S, 16R/4M/40S, and 20R/0M/40S, respectively. These strains can even infect some accessions containing effective resistant genes (Xa4 or Xa7).

scholarly journals HERITABILITAS, SUMBER GEN, DAN DURABILITAS KETAHANAN VARIETAS PADI TERHADAP PENYAKIT HAWAR DAUN BAKTERI/ Heritability, Gene Resource, and Durability of Rice Varieties Resistance To Bacterial Leaf Blight Disease

2017 ◽  
Vol 36 (2) ◽  
pp. 99
Author(s):  
Dini Yuliani ◽  
Wage Ratna Rohaeni
Keyword(s):  
Genetic Resistance ◽  
Female Parent ◽  
Leaf Blight ◽  
Rice Production ◽  
Bacterial Leaf Blight ◽  
Sources Of Resistance ◽  
Rice Varieties ◽  
Resistant Varieties ◽  
Blight Disease ◽  
Resistant Progeny

<p>Bacterial leaf blight (BLB) disease is one of the obstacles in increasing of rice production. The use of resistant varieties is an effective and easy to implement for farmers. This paper discusses the heritability and source of resistance genes of rice varieties against the BLB disease and strategies to maintain the durability of resistant varieties as one of the control efforts through plant breeding to supports the increasing of rice production. Assembling and development of resistant varieties play an important role in controlling BLB disease because it has a genetic resistance<br />mechanism that can be inherited to progeny level. Varieties with vertical resistance are easily broken by pathogens, so it is necessary to assembling of varieties with horizontal resistance. To obtain the resistant progeny to BLB disease in the assembly of varieties, the position of the resistant varieties should be played as a female parent that has a high specific joining power. The nature of resistance to BLB is from a population whose parent genes are derived from multiple cross results has higher heritability. The populations derived from a double-cross have multigenic resistance and have the potential to produce recombinant individuals resistant for prolonged periods (durable). The availability of durable resistant varieties become a key requirement in sustainable BLB disease control. This matter can be done by improving the resistance of varieties through the assembling of varieties with various sources of resistance such as wild rice, local rice, and introduced rice.</p><p>Keywords: Rice, varieties, resistance, bacterial leaf blight, durability, heritability</p><p> </p><p><strong>ABSTRAK</strong></p><p>Penyakit hawar daun bakteri (HDB) merupakan salah satu kendala dalam peningkatan produksi padi. Penggunaan varietas tahan merupakan cara pengendalian yang efektif dan mudah diterapkan petani. Tulisan ini membahas heritabilitas dan sumber gen ketahanan varietas padi terhadap penyakit HDB dan strategi mempertahankan durabilitas varietas tahan sebagai salah satu upaya pengendalian melalui pemuliaan tanaman mendukung upaya peningkatan produksi padi. Perakitan dan pengembangan varietas tahan berperan penting mengendalikan penyakit HDB, karena memiliki mekanisme ketahanan genetik yang dapat diwariskan kepada keturunannya. Varietas dengan ketahanan vertikal mudah dipatahkan oleh patogen, sehingga perlu upaya perakitan varietas dengan ketahanan horizontal. Untuk memperoleh keturunan tanaman padi yang tahan terhadap penyakit HDB dalam perakitan varietas, posisi tetua tahan sebaiknya diperankan sebagai tetua betina yang memiliki daya gabung khusus yang tinggi. Sifat ketahanan HDB dari populasi tetua yang mengandung gen dari hasil silang ganda memilliki heritabilitas lebih tinggi. Populasi turunan dari silang ganda memiliki ketahanan multigenik dan berpeluang menghasilkan individu rekombinan tahan untuk periode yang lama (durable). Ketersediaan varietas tahan yang durable menjadi syarat utama dalam pengendalian penyakit HDB secara berkelanjutan. Hal ini dapat dilakukan dengan perbaikan ketahanan varietas melalui perakitan varietas dengan berbagai sumber ketahanan, di antaranya padi liar, padi lokal, dan padi introduksi.</p><p>Kata kunci: Padi, varietas, ketahanan, hawar daun bakteri, durabilitas, heritabilitas</p>

scholarly journals Bioefficacy of Chemicals against Bacterial Leaf Blight Disease of Rice

2020 ◽  
Vol 9 (6) ◽  
pp. 3570-3575
Author(s):  
A. Khandual ◽  
M. K. Mishra ◽  
H. Swain ◽  
S. Mohanty ◽  
P. C. Rath ◽  
...  
Keyword(s):  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Blight Disease

scholarly journals Phenotypic Screening of F4 Breeding Lines against Bacterial Blight Disease in Rice

2021 ◽  
pp. 299-304
Author(s):  
K. Alekya ◽  
B. Laxmi Prasanna ◽  
M. Balram ◽  
N. Balram ◽  
P. Gonya Nayak ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Agricultural Research ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Research Station ◽  
Rice Grain ◽  
Disease Score ◽  
Breeding Lines ◽  
Biotic Stresses ◽  
Resistant Reaction

Biotic stresses are major threat to rice production. Among biotic stresses, bacterial leaf blight is one of the major diseases affecting rice grain production in rice growing areas. Present investigation was conducted to evaluate phenotypic effect of 50 breeding lines from a cross (Pranahitha//ISM/MTU1010) in glass house at Regional Agricultural Research Station, Jagtial by inoculating Bacterial leaf blight culture (DX-020) by leaf clipping method. Out of these 50 F4 lines evaluated, twenty nine breeding lines showed resistant reaction with disease score of 1. Eleven lines showed moderately resistant reaction with disease score 3. Twenty nine breeding lines that were resistant with disease score 1, had excellent grain yield. Hence, these lines can be advanced to further generations. Thus the present study has demonstrated that phenotypic selection is successful in the glass house and these breeding lines with higher yield levels are expected to perform better in the field trials and further in the farmers fields with the good level of bacterial blight resistance.

Riboflavin (Vitamin B2) mediated defence induction against bacterial leaf blight: probing through chlorophyll a fluorescence induction O–J–I–P transients

2018 ◽  
Vol 45 (12) ◽  
pp. 1251 ◽  
Author(s):  
Shasmita ◽  
Harekrushna Swain ◽  
Anuprita Ray ◽  
Pradipta K. Mohapatra ◽  
Ramani K. Sarkar ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Oryza Sativa L ◽  
Bacterial Pathogen ◽  
Fluorescence Induction ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Yield Data ◽  
Progress Curve ◽  
Moderately Resistant

Bacterial leaf blight (BLB) is a serious threat for rice (Oryza sativa L.) cultivation caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae. The pathogen mainly damages the leaf chlorophyllous tissue, resulting in poor photosynthesis and causing up to 50% reductions in grain yield. In the present work, we have compared the structural and functional ability of the chloroplast of three varieties of rice with different degrees of susceptibility (TN1, highly susceptible; IR-20, moderately resistant; DV-85, resistant to BLB) treated with riboflavin (1 and 2 mM) and infected with BLB, with chlorophyll fluorescence as a tool. As indicated by the chlorophyll fluorescence technique, the disease progress curve and yield data, riboflavin acted as an effective vitamin for inducing resistance against BLB. Plants treated with riboflavin showed improved PSII activity, more chlorophyll content and higher yield than the diseased plants.

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