scholarly journals Phenotypic Screening of F4 Breeding Lines against Bacterial Blight Disease in Rice

2021 ◽  
pp. 299-304
Author(s):  
K. Alekya ◽  
B. Laxmi Prasanna ◽  
M. Balram ◽  
N. Balram ◽  
P. Gonya Nayak ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Agricultural Research ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Research Station ◽  
Rice Grain ◽  
Disease Score ◽  
Breeding Lines ◽  
Biotic Stresses ◽  
Resistant Reaction

Biotic stresses are major threat to rice production. Among biotic stresses, bacterial leaf blight is one of the major diseases affecting rice grain production in rice growing areas. Present investigation was conducted to evaluate phenotypic effect of 50 breeding lines from a cross (Pranahitha//ISM/MTU1010) in glass house at Regional Agricultural Research Station, Jagtial by inoculating Bacterial leaf blight culture (DX-020) by leaf clipping method. Out of these 50 F4 lines evaluated, twenty nine breeding lines showed resistant reaction with disease score of 1. Eleven lines showed moderately resistant reaction with disease score 3. Twenty nine breeding lines that were resistant with disease score 1, had excellent grain yield. Hence, these lines can be advanced to further generations. Thus the present study has demonstrated that phenotypic selection is successful in the glass house and these breeding lines with higher yield levels are expected to perform better in the field trials and further in the farmers fields with the good level of bacterial blight resistance.

scholarly journals Development of New Rice (Oryza. sativa L.) Breeding Lines through Marker-Assisted Introgression and Pyramiding of Brown Planthopper, Blast, Bacterial Leaf Blight Resistance, and Aroma Genes

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2525
Author(s):  
Xuan Wang ◽  
Xinying Guo ◽  
Xixi Ma ◽  
Liang Luo ◽  
Yaoyu Fang ◽  
...  
Keyword(s):  
High Resistance ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Brown Planthopper ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Breeding Method ◽  
Breeding Lines ◽  
Biotic Stresses ◽  
Multiple Genes

Brown planthopper, blast, and bacterial blight are the main biotic stressors of rice and can cause a massive loss in rice production. Aroma is an important character of rice quality. It is of far-reaching significance to breed resistant and high-quality varieties using germplasms with objective genes. In this study, the introgression and pyramiding of brown planthopper (BPH), blast, and bacterial leaf blight (BLB) resistance genes and aroma genes into elite rice maintainers and restorers were conducted through conventional cross-breeding coupled with the marker-assisted selection (MAS) breeding method. Single-plant selection was performed from F2 onwards to select desirable recombinants possessing alleles of interest with suitable phenotypes. Respective linked markers were used in each generation from intercrossing to the F7 generation for tracking the presence of targeted genes. A total of 74 improved lines (ILs) have been developed which possess a combination of 1 to 4 genes for BPH, blast, and BLB resistance and aroma. These ILs showed moderate to high resistance to multiple biotic stresses (BPH, blast and BLB) or aromatic fragrance without obvious negative effects on agronomic traits. As multiple resistance and aromatic traits have become significant objectives in rice breeding, these resistance and/or aroma gene introgressed or pyramided lines have important application prospects. Core ideas: (1) marker-assisted breeding was used to pyramid multiple genes for an elite breeding line; (2) improved lines with the introgression of 1–4 genes were developed to achieve high resistance against various biotic stresses and aroma; (3) new lines were used as donor parents to introgress multiple genes in other genetic backgrounds.

Performance of Breeding Lines/Cultivars of Rice Against Bacterial Leaf Blight and Sheath Rot under Field Condition

2003 ◽  
Vol 3 (5) ◽  
pp. 478-483
Author(s):  
Mosharraf Hossain . ◽  
Shah Md. Munirur Rah . ◽  
K.M. Khalequzzaman . ◽  
Md. Nazrul Islam . ◽  
M. Ashrafuzzaman .
Keyword(s):  
Field Condition ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Breeding Lines ◽  
Sheath Rot

scholarly journals Response of Rice Varieties to Age of Seedlings and Transplanting Dates

1970 ◽  
pp. 14-17
Author(s):  
Mishri L Shah ◽  
Rambaran Yadav
Keyword(s):  
Grain Yield ◽  
Interaction Effect ◽  
Management Practices ◽  
Agricultural Research ◽  
Research Station ◽  
Rice Grain ◽  
Rice Varieties ◽  
Research Journal ◽  
Key Words Age ◽  
Similar Yield

A field experiment was conducted at Regional Agricultural Research Station, Parwanipur in 1998/99 and 1999/00 with an aim to find out the alternate management practices to compensate the loss in the grain yield due to flood. Irrespective of the varieties and transplanting dates, age of seedlings had no effect on grain yield of rice. But transplanting dates had significant effect on grain and grain contributing characters. The yield of rice transplanted at 1 Sept was 25.6 and 37.5% less in 1998/99 and 1999/00 respectively as compared to rice grain yield of 14 July transplanting. Radha 11 registered the highest grain yield of 4086 kg ha-1 in 1999/00 and 2662 kg ha-1 in 1998/99, which was at par to the yield obtained by Sabitri at the same year. The interaction effect of the age of seedlings, transplanting dates and varieties were found significant in both the years. 25 days old seedlings transplanted on 14 July in 1999/00 of rice varieties Masuli and Radha 11, produced statistically the similar yield. Radha 11 was the best among the tested varieties. 25 days old seedlings of Masuli, Basmati, Sabitri and Radha 11 can be recommended to transplant as late as Sept 1 .Key words: Age of seedlings; Rice; Transplanting datesDOI: http://dx.doi.org/10.3126/narj.v4i0.4858Nepal Agriculture Research Journal Vol. 4&5, 2001/2002Page: 14-17Uploaded date: 7 June, 2011

scholarly journals First Draft Genome Sequence of Xanthomonas axonopodis pv. eucalyptorum, Causal Agent of Bacterial Leaf Blight on Eucalypt

2019 ◽  
Vol 8 (16) ◽  
Author(s):  
Yane F. Neves ◽  
Samuel A. Santos ◽  
Lúcio M. S. Guimarães ◽  
Pedro M. P. Vidigal ◽  
Jorge L. Badel ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Bacterial Blight ◽  
Molecular Mechanisms ◽  
Draft Genome ◽  
Genomic Data ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Xanthomonas Axonopodis

Here, we report the annotated draft genome sequence of Xanthomonas axonopodis pv. eucalyptorum pathotype strain LPF602 (synonym Xanthomonas axonopodis BSC45a), isolated from eucalypt leaves showing bacterial blight symptoms in Brazil. The availability of these genomic data will help improve the understanding of the evolution and molecular mechanisms involved in the pathogenesis of this microorganism.

scholarly journals Management of Leaf Blight of Garlic with Fungicides in Central Tarai of Nepal

2014 ◽  
Vol 8 ◽  
pp. 63-66
Author(s):  
Prem C.P. Chaurasia ◽  
Jang B. Prasad ◽  
Aanandi Mandal
Keyword(s):  
Plant Height ◽  
Agricultural Research ◽  
Block Design ◽  
Leaf Blight ◽  
Research Station ◽  
Insignificant Effect ◽  
Agricultural Research Station ◽  
Randomized Block Design ◽  
Bulb Yield

Management of purple blotch in garlic was carried out in 2005/06 and 2006/07 at horticultural farm of Regional Agricultural Research Station, Parwanipur in Complete Randomized Block Design with three replications. First year’s results indicated insignificant effect of fungicides on Percentage Disease Intensity (PDI) of purple blotch disease of garlic as number of sprays used seemed to be inadequate. Second year’s result revealed that two sprays of Bavistin @ 0.2% had lowest PDI value but at par to other different number of sprays of different fungicides except no spray of fungicide. Bulb yield was highest in two sprays of Dithane M-45 @ 0.3% followed by two sprays of Krinoxyl @ 0.15%, three sprays of Blitox-50 @ 0.3%. Plant height was insignificant and highest bulb weight was found with three sprays of Blitox-50 followed by two sprays of Bavistin. Two year’s combined results indicated that two sprays of Bavistin @ 0.2% had lowest PDI value and all others PDI values were at par except control. The highest bulb yield was given by two sprays of Dithane M-45 followed by three sprays of Bavistin. Plant height and bulb weight were insignificant. It can be recommended that purple blotch disease can be managed by spraying Bavistin @ 0.2% thrice at 15 days interval or any other tested fungicides to have less disease, higher bulb yield and more economic return.Nepal Agric. Res. J. Vol. 8, 2007, pp. 63-66DOI: http://dx.doi.org/10.3126/narj.v8i0.11582 

scholarly journals Effect of Potassium Fertilizer on Development of Bacterial Blight of Rice

1970 ◽  
Vol 46 (1) ◽  
pp. 69-76
Author(s):  
N Begum ◽  
MM Rahman ◽  
MA Bashar ◽  
MA Hossain ◽  
MN Uddin
Keyword(s):  
Disease Severity ◽  
Bacterial Blight ◽  
Oryza Sativa L ◽  
Block Design ◽  
Maximum Yield ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Growth Stages ◽  
Potassium Fertilizer ◽  
Bacterial Blight Of Rice

Effect of five potassium doses viz, recommended dose (RD) (40 kg Murate of Potash(MP)/hector), RD + 10 kg MP/ha, RD + 20 kg MP/ha, RD + 30 kg MP/ha, RD + 40 kg MP /ha to manage bacterial blight of rice were studied during Boro season in 2005. The experiment was laid in Randomized Completely Block Design (RCBD) having three replications. Highest disease severity (55.92%) was observed due to bacterial leaf blight at maximum tillering stage when applied 30 kg MP/ha additional potash. Lowest disease severity (0.45%) of bacterial leaf blight were recorded from the plot where no additional MP was applied but highest yield (4.35 tones/ha) was observed in the plot where additional 20 kg MP/ha was applied. The amount of potassium in the leaves did not vary significantly even after additional application of potassium either 30 or 20 kg MP/ha though it was more (1.31%) when applied 40 kg MP/ha. In another experiment in the present study the lowest disease severity 15.22% and 10.53% were recorded when additional 5kg MP /ha was applied seven days before inoculation at active tillering and panicle initiation stages, respectively. Disease severity was lower in single inoculation compare to multiple inoculations except the plot where 5kg/ha additional potash applied three days before bacterial inoculation. Maximum yield (3.86, 4.38, 4.64 t/ha) was recorded when inoculated at active tillering, panicle initiation and flowering stages separately if potassium was applied seven days before inoculation than those of multiple inoculations. The results suggested that potassium top dressing just before disease initiation was good for higher yield and less disease development. However inoculation in all the growth stages of the crop gave lower yields even potassium application was done at seven or three days before or after inoculation. Key words: Bacterial leaf blight; Oryza sativa L.; Potassium fertilizer DOI: http://dx.doi.org/10.3329/bjsir.v46i1.8109 Bangladesh J. Sci. Ind. Res. 46(1), 69-76, 2011

scholarly journals Identification of Rice Genotypes Resistant To Bacterial Leaf Blight Disease Using Ssr Markers

2018 ◽  
Vol 35 (1) ◽  
pp. 113-120
Author(s):  
A. Acharya ◽  
N. R. Adhikari ◽  
R. B. Amgain ◽  
A. Poudel ◽  
R. Yadav ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Bacterial Blight ◽  
Gene Pyramiding ◽  
Bacterial Pathogen ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Resistant Varieties ◽  
Blight Disease ◽  
Almost All

 Bacterial blight disease of rice is a growing and challenging concern in Nepal. Since bacterial pathogen (Xanthomonas oryzae pv. oryzae) is difficult to manage by other means effectively, development of host plant resistance is the most effective mean to control this disease. This study was carried out to identify the bacterial leaf blight resistant genotypes of rice by using of molecular markers linked with bacterial blight resistant genes. Sixty genotypes of rice were screened at glass house for BB resistance and they were tested for the presence of Xa4, Xa5, Xa7 and Xa21 genes using markers MP, RM122, M5 and pTA248 respectively. IRBB 60 and Jumli Marshi were used as resistant and susceptible check respectively. Twenty five genotypes of rice were detected with presence of Xa4 gene, 24 genotypes with Xa5 gene and fourteen genotypes with Xa7 gene. Twenty four genotypes did not showed presence of any gene. Twenty four genotypes showed the presence of more than one gene with the specific molecular markers. Almost all genotypes that did not show presence of any gene were found highly susceptible in greenhouse conditions with both inoculums. Genotypes having more than one BB resistance gene were found resistant in greenhouse conditions with both inoculums suggesting combination of BB resistance gene through gene pyramiding will provide BB resistant varieties in rice breeding.

scholarly journals Occurrence of a Subpopulation of Xanthomonas oryzae pv. oryzae with Virulence to Rice Cv. IRBB21 (Xa21) in Southern India

Plant Disease ◽  
1999 ◽  
Vol 83 (8) ◽  
pp. 781-781 ◽  
Author(s):  
V. Brindha Priyadarisini ◽  
S. S. Gnanamanickam
Keyword(s):  
Bacterial Blight ◽  
Agricultural Research ◽  
Oryza Sativa L ◽  
Xanthomonas Oryzae ◽  
Southern India ◽  
Lesion Length ◽  
Research Station ◽  
Cornell University ◽  
Bacterial Blight Pathogen ◽  
Kerala State

About 200 isolates of the bacterial blight pathogen Xanthomonas oryzae pv. oryzae were obtained from infected rice (Oryza sativa L.) leaf samples from the Palghat District of Kerala State in southern India during January and February, 1998, when there was a severe epidemic of bacterial blight in the farmers' fields. One hundred and forty of these isolates were assayed for pathogenicity on rice cv. IR24 (no R gene), IRBB21 (Xa21), and NH56 (Xa4+xa5+xa13+Xa21) at their maximum tillering stage by the standard clip-inoculation technique (1) at the Regional Agricultural Research Station (Kerala Agricultural University), Pattambi. In two separate experiments, all the isolates were found to be pathogenic to IR24, producing lesions greater than 10 cm in length. None of the isolates induced susceptible symptoms on cv. NH56 and the average lesion length was less than 2 cm. Twenty of the 140 isolates, however, showed virulence to IRBB21 (seeds supplied by S. R. McCouch, Cornell University, and by P. Ronald, University of California-Davis) and induced lesions of 10.0 to 18.0 cm. We report here the presence of Indian races of X. oryzae pv. oryzae that are virulent to IRBB21 (Xa21), contradicting an earlier report of avirulence (2). These results suggest that the deployment of Xa21 alone will not be a sound strategy for the management of bacterial blight in southern India. References: (1) S. S. Gnanamanickam et al. Plant Dis. 78:173, 1994. (2) G.-L.Wang et al. Mol. Plant-Microbe Interact. 9:850, 1996.

scholarly journals Effect of Inoculation of Plant Growth Promoting Rhizobacteria (PGPR) Mix I Formulations on Plant Growth, Yield, Disease Incidence and Disease Severity of Rhizoctonia Leaf Blight of Amaranthus (Amaranthus tricolor L.)

2021 ◽  
Author(s):  
A.R. Resmi ◽  
B. Lovely ◽  
A. Jayapal ◽  
G. Suja ◽  
N. Chitra
Keyword(s):  
Plant Growth ◽  
Disease Severity ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Growth Yield ◽  
Plant Growth Promoting Rhizobacteria ◽  
Leaf Blight ◽  
Research Station ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background: Amaranthus is the most popular and commercially cultivated leafy vegetable in the Southern part of India, especially Tamil Nadu and Kerala which is susceptible to a number of diseases. Among the different diseases affecting amaranth, foliar blight caused by Rhizoctonia solani Kuhn, is considered as the most serious disease in Kerala. Methods: A field experiment was taken up at Onattukara Regional Agricultural Research Station (O.R.A.R.S), Kayamkulam, Alappuzha, Kerala during December 2019 to February 2020 to assess the influence of dust and liquid formulations of Plant Growth Promoting Rhizobacteria (PGPR) mix I on growth, yield and disease incidence (Rhizoctonia leaf blight) in amaranthus. Result: The results of the study reveal that maximum number of leaves, number of branches per plant and yield were produced by the plants that were subjected to seedling root dip with 5% talc formulation followed by drenching with 5% talc solution at 30 DAT and 45 DAT. Regardless of talc or liquid formulation of PGPR mix I (2%) seedling dip followed by drenching at 15, 30 and 45 DAT provided the least disease incidence and disease severity in amaranthus at Onattukara condition. Hence use of PGPR mix I is a prerequisite for effective growth, yield and management of leaf blight of amaranthus at Onattukara.

scholarly journals Phenotypic Evaluation of Advanced Breeding Lines for Resistance against Bacterial Leaf Blight Disease in Rice (Oryza sativa L.)

2021 ◽  
pp. 305-309
Author(s):  
V. Srujana ◽  
M. Balram ◽  
B. Srinivas ◽  
N. Balram
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Leaf Blight ◽  
Rice Crop ◽  
Bacterial Leaf Blight ◽  
Blight Resistance ◽  
Breeding Lines ◽  
Blight Disease

Bacterial Leaf Blight disease epidemically damaged the rice crop. Absence of resistance against BLB disease in MTU1010 is one of the main reason for these epidemics. Thus, there is urgent need to search for resistant source and subsequently, their phenotypic evaluation to validate the resistant source. In this paper discussing about the highlights of phenotypic evaluation of Bacterial Leaf Blight resistance in MTU1010 NIL variety of rice. In the present study, fifty advanced breeding lines along with parents i.e., MTU1010 NIL X Akshayadhan NIL, were screened for bacterial leaf blight disease, Thirty-four breeding lines were shown resistance against bacterial leaf blight disease.

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