scholarly journals Blood glucose concentration measured in EDTA/F plasma and serum in a referral clinical laboratory in Nepal

2021 ◽  
Vol 11 (1) ◽  
pp. 1837-1841
Author(s):  
Vivek Pant ◽  
Keyoor Gautam ◽  
Santosh Pradhan ◽  
Devish Pyakurel ◽  
Abha Shrestha
Keyword(s):  
Blood Glucose ◽  
Sodium Fluoride ◽  
Plasma Glucose ◽  
Glucose Concentration ◽  
Clinical Laboratory ◽  
Blood Glucose Concentration ◽  
Serum Glucose ◽  
Relative Reduction ◽  
The Difference ◽  
Glucose Estimation

Background: Sodium fluoride tubes or serum separator tubes are mostly used for blood glucose estimation in the clinical laboratories of Nepal. The study aimed to investigate the stability of glucose in samples collected in serum separator tubes and sodium fluoride/sodium ethylenediaminetetraacetic tubes by comparing the glucose concentration at 30 minutes and 4 hours collected and handled differently to simulate prolonged sample transport between venipunctures, centrifugation, and measurement.Materials and Methods: Samples were collected from healthy volunteers into two different serum separator tubes and two different sodium fluoride/sodium ethylenediaminetetraacetic tubes. Glucose concentration was measured at 30 minutes after venipuncture and compared with results from the same samples analyzed at four hours and with the results from tubes centrifuged with a delay of 4 hours. Differences between baseline and respective delayed analyzed glucose values for each tube type were tested using the Student’s paired t-test and Deming regression.Results: When comparing plasma glucose at 30 minutes, glycolysis caused a relative reduction of the glucose concentration in serum at 30 minutes of 3.1 %, which is only slightly less than at 4 hours (3.7 %). This is still substantially more than the reduction in plasma at 4 hours (1.3 %). Surprisingly, the difference between plasma glucose at 30 minutes and serum glucose at 4 hours was only 1.9% which is not clinically significant.Conclusions: The Na-F/Na2 EDTA tubes and serum separator tubes can be used interchangeably for analysis of blood glucose up to 4 hours if centrifuged within 30 minutes.

Importance of blood glucose concentration in regulating lipolysis during fasting in humans

1990 ◽  
Vol 258 (1) ◽  
pp. E32-E39 ◽  
Author(s):  
S. Klein ◽  
O. B. Holland ◽  
R. R. Wolfe
Keyword(s):  
Blood Glucose ◽  
Plasma Glucose ◽  
Glucose Concentration ◽  
Plasma Insulin ◽  
Blood Glucose Concentration ◽  
Glucose Infusion ◽  
Short Term ◽  
Epinephrine Infusion ◽  
Isotopic Studies ◽  
Fasting Plasma Insulin

The importance of the decline in blood glucose concentration on lipolysis and the lipolytic effect of epinephrine was evaluated during short-term fasting. Lipolytic rates were determined by infusing [2H5]glycerol and [1-13C]palmitic acid. Five volunteers were studied after 12 h of fasting before and during epinephrine infusion and after 84 h of fasting, before and during glucose infusion when plasma glucose was restored to postabsorptive values, and during glucose plus epinephrine infusion. In another protocol, five volunteers were given glucose intravenously throughout fasting to maintain plasma glucose at postabsorptive levels and isotopic studies were performed after 12 and 84 h of fasting before and during epinephrine infusion. Glucose infusion after 84 h of fasting restored glucose and insulin concentrations and lipolytic rates toward 12-h fasting values. When euglycemia was maintained throughout fasting, plasma insulin still declined (P less than 0.05) and lipolytic rates still increased (P less than 0.05). Despite similar glucose concentrations, the lipolytic response to epinephrine infusion was greater after 84 h than after 12 h of fasting in both protocols (P less than 0.05). These studies demonstrate that the decline in plasma glucose contributes to, but is not required for, the increase in lipolysis during fasting. The increase in epinephrine-stimulated lipolysis that occurs during fasting is not dependent on a decrease in plasma glucose concentration.

scholarly journals The Consequences of Prior Initiation of Breast Feeding on Blood Glucose Levels in Neonates Born In a Tertiary Care Hospital

2020 ◽  
Vol 8 (2) ◽  
pp. 23-29
Author(s):  
Rizwan-U- Zama ◽  
Ayesha Siddiqa ◽  
A.N. Thobbi ◽  
Tehseen Sajid Mudhol ◽  
R Shruthi
Keyword(s):  
Blood Glucose ◽  
Birth Weight ◽  
Plasma Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Mode Of Delivery ◽  
Postnatal Life ◽  
Care Hospital ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Hypoglycemia is the most common event of failure of metabolic adjustments in the newborn. Changes in maternal and fetal monitoring techniques, administration of glucose-containing solutions during labor, delivery and early feeding in neonates significantly alter blood glucose concentrations during the first week of postnatal life. Subjects and Methods: A total of 90 healthy (60 born by FTND, 30 born by LSCS) term, AGA infants were longitudinally evaluated at birth, at one hour after feeds (post feed), and after 6 hours of life. Plasma glucose was estimated from Heel Prick capillary samples by glucometer method. The influence of mode of delivery, the interval between feeds, sex, birth weight, on blood glucose was analyzed. Results: The way of delivery did not affect the plasma glucose concentration in neonates. There was a substantial increase in blood glucose concentration after the first feed irrespective of their birth weight. It was found that female babies had a higher blood glucose concentration than male babies during our study period. All babies maintained normal blood glucose with the continuation of breastfeeding. Conclusion: Plasma glucose levels are satisfactorily maintained in healthy term infants without resort to pre-lacteal feeds and mode of delivery did not influence plasma glucose. There is no need to check blood glucose levels routinely in an asymptomatic, healthy, term, breastfed infants.

Renal Glycosuria in Pyelonephritis

PEDIATRICS ◽  
1989 ◽  
Vol 83 (4) ◽  
pp. 652-653 ◽  
Author(s):  
HOWARD TRACHTMAN ◽  
RONALD KAHN
Keyword(s):  
Differential Diagnosis ◽  
Blood Glucose ◽  
Acute Pyelonephritis ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Pediatric Nephrology ◽  
Serum Glucose ◽  
Renal Glycosuria

Standard pediatric nephrology textbooks do not include acute pyelonephritis in the differential diagnosis of renal glycosuria. Recently, we encountered a 9-month-old girl who had fever up≤39.4°C(103°F) for five to six days. On the sixth day of her illness, a Chemstrip urinalysis was performed and demonstrated the presence of 2+ pyuria, 1+ protein, 1+ hematuria, and 4+ glycosuria. A simultaneous blood glucose concentration was 107 mg/ dL. A repeat urinalysis, done 32 hours after a feeding, confirmed the presence of renal glycosuria, a concurrent serum glucose of 129 mg/dL, pyuria, minimal hematuria, and proteinuria.

scholarly journals Effects of dietary manipulations and glucose infusion on glucagon response during exercise in rats

1997 ◽  
Vol 83 (1) ◽  
pp. 148-152 ◽  
Author(s):  
Maurice Tadjoré ◽  
Raynald Bergeron ◽  
Martin Latour ◽  
François Désy ◽  
Claude Warren ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Plasma Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Glucose Infusion ◽  
Glucose Response ◽  
Glucose Levels ◽  
Arterial Blood ◽  
Blood Glucose Levels ◽  
Exercise Period

Tadjoré, Maurice, Raynald Bergeron, Martin Latour, François Désy, Claude Warren, and Jean-Marc Lavoie.Effects of dietary manipulations and glucose infusion on glucagon response during exercise in rats. J. Appl. Physiol. 83(1): 148–152, 1997.—The purpose of the present investigation was to test the hypothesis that blood glucose concentration is not always related to glucagon response during exercise. Three groups of rats were submitted to a prolonged (3-h) swimming exercise. Two groups of rats had their normal food intake restricted by 50% the night before the experiment. One of these two groups of rats was intravenously infused with glucose throughout exercise to maintain euglycemia. The third group of rats swam while under normal dietary conditions. Plasma glucose, sampled in arterial blood, was reduced ( P < 0.05) at 75, 105, 150, and 170 min of exercise (from ∼130 to 110 mg/dl) in the food-restricted animals without glucose infusion, whereas a significant ( P < 0.05) increase was measured in the two other groups during exercise. A significant ( P < 0.01) difference in the mean integrated areas under the glucose-concentration curve was found only between the fed and the two food-restricted groups. Plasma insulin concentrations decreased ( P < 0.05) similarly in all groups during exercise, whereas plasma epinephrine and norepinephrine concentrations increased significantly ( P < 0.01) in all groups. Despite differences between groups in plasma glucose response during exercise, and despite the absence of any decrease in exercising blood glucose levels in at least two of the three groups, plasma glucagon responses were increased ( P < 0.05) similarly in all groups (from ∼250 to 550 pg/ml) at the end of the exercise period. The increase in glucagon was significant after 90 min of exercise in the food-restricted groups, with or without glucose infusion, but only after 140 min in the fed group. These results indicate that the glucagon response during exercise is not always linked to the decrease in plasma glucose.

scholarly journals Incidence of glucose level abnormalities in neonatal sepsis and its association with mortality

2020 ◽  
Vol 7 (12) ◽  
pp. 2280
Author(s):  
K. S. L. Parvathi ◽  
Santosh Kumar Soma ◽  
Prasad Thanda
Keyword(s):  
Glucose Level ◽  
Plasma Glucose ◽  
Neonatal Sepsis ◽  
Prospective Observational Study ◽  
Serum Glucose ◽  
Glucose Levels ◽  
Mortality Outcome ◽  
The Difference ◽  
Glucose Estimation ◽  
Glucose Abnormalities

Background: Plasma glucose abnormalities were previously noted in neonatal sepsis, but data in neonates is limited and the association with mortality is not established. The aim of the study was to determine the incidence of plasma glucose abnormalities among newborns with sepsis and their association with mortality.Methods: This was a prospective observational study including 50 neonates with suspected, probable and proven sepsis. Plasma glucose level was measured within 2 hours of admission and the patients were monitored till discharge or death. The patients were divided into hyperglycaemic, hypoglycaemic and normoglycemic subgroups as per the serum glucose levels.Results: Majority (56%) were noted to have normoglycemia, followed by hypoglycaemia in 32% and 12% had hyperglycaemic. Mortality in the hypoglycemic, hyperglycemic, and normoglycemic subgroups were 50.0, 33.3, and 7.2% respectively. Mortality was high in hyperglycemic patients compared to normoglycemic patients but the difference was not statistically significant between two groups, whereas the mortality was high in hypoglycemic patients compared to normoglycemic patients and the difference was statistically significant between two groups. A significant association was noted between hypoglycemia in neonatal sepsis with mortality.Conclusions: Altered glycemic status is common in neonatal sepsis. Mortality is higher among septic neonates with hypoglycemia. We conclude that majority of septic neonates had high mortality rate when plasma glucose levels were either >145 mg/dl or <45 mg/dl. This signifies the importance of plasma glucose estimation in cases of neonatal sepsis to improve mortality outcome. 

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