scholarly journals Isolation and Culture Condition Optimization of Chlorella vulgaris

2014 ◽  
Vol 14 (2) ◽  
pp. 43-48
Author(s):  
U Rai ◽  
G Deshar ◽  
B Rai ◽  
K Bhattarai ◽  
RP Dhakal ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Specific Growth ◽  
Dark Period ◽  
Nitrogen Sources ◽  
Pilot Scale ◽  
Air Flow Rate ◽  
Mixotrophic Growth ◽  
Condition Optimization ◽  
Autotrophic Condition ◽  
Culture Condition Optimization

Chlorella vulgaris Beyerinck (Beijerinck) is one of the fast growing planktonic microalgae predominantly found in lentic habitats. It was isolated from water samples using BG 11 medium and identified and screened for mixotrophic growth using glucose as carbon source in the medium. The growth of this algae was also studied for autotrophic and mixotrophic regimen. It showed higher growth in a medium of pH 7 containing glucose rather than in autotrophic condition. Similarly, its growth was also studied in media containing different nitrogen sources in which NaNO3 gave the best result. A pilot scale culture of C. vulgaris was performed in 5 l fermenter illuminated with fluorescent tube of 36 W maintaining 16:8 hrs of light and dark period with pH 7, temperature at 25°C, stirrer rate at 75 rpm, air flow rate at 30 L/hr. The specific growth rate was found to be 6.879 cells/ ml/ day whereas doubling time was found to be 2 hrs and 9 mins. The cells were harvested by sedimentation and centrifugation followed by drying at 50°- 80°C. The dried biomass was treated with dichloromethane: methanol (2:1) v/v solution in Soxhlet extractor for 3 DOI: http://dx.doi.org/10.3126/njst.v14i2.10414   Nepal Journal of Science and Technology Vol. 14, No. 2 (2013) 43-48

Influence of Heavy Metals on the Growth of Chlorella vulgaris

2021 ◽  
Vol 15 (3) ◽  
pp. 374-379
Author(s):  
Bingbing Pang ◽  
Mingzhou Zeng ◽  
Wenjia Zhang ◽  
Fengcai Ye ◽  
Changhua Shang
Keyword(s):  
Heavy Metals ◽  
Water Quality ◽  
Growth Rate ◽  
Growth Inhibition ◽  
Chlorella Vulgaris ◽  
Specific Growth ◽  
Concentration Level ◽  
Water Quality Monitoring ◽  
Quality Monitoring ◽  
Average Specific Growth Rate

Growth inhibition of chromium (Cr), cadmium (Cd) and lead (Pb) to fresh water microalga Chlorella vulgaris (C. vulgaris) FACHB-8 was examined. These results demonstrated that the concentration level (EC50 value) of three heavy metals (Cr, Cd and Pb) could be utilized as an indicator for evaluating the toxicities of Cr, Cd and Pb for microalga growth. The EC50 values of Cr for C. vulgaris were 0.22, 0.07 and 0.04 mg/L at 24, 48 and 72 h based on Algorithm 2 (%Ir, percent inhibition in average specific growth rate), respectively. The EC50 values of Cd for C. vulgaris were 2.76, 1.08 and 0.93 mg/L at 24, 48 and 72 h based on Algorithm 2, respectively. The EC50 values of Pb for C. vulgaris were 73.21, 65.02 and 48.38 mg/L at 24, 48 and 72 h based on Algorithm 2, respectively. The results laid a good foundation for the application of C. vulgaris in the water quality monitoring.

Pilot-scale production of lutein using Chlorella vulgaris

2019 ◽  
Vol 44 ◽  
pp. 101707 ◽  
Author(s):  
Dale D. McClure ◽  
Jonathan K. Nightingale ◽  
Audrey Luiz ◽  
Sachin Black ◽  
Jingyuan Zhu ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Pilot Scale ◽  
Scale Production ◽  
Pilot Scale Production

Cyanobacterial extract as a source of nutrients for mixotrophic growth of Chlorella vulgaris and Nannochloropsis oculata

2019 ◽  
Vol 39 ◽  
pp. 101480
Author(s):  
Samira Rezasoltani ◽  
Farzaneh Vahabzadeh ◽  
Zeinab Shariatmadari ◽  
Faezeh Ghanati
Keyword(s):  
Chlorella Vulgaris ◽  
Nannochloropsis Oculata ◽  
Mixotrophic Growth

Biomass production in Chlorella vulgaris biofilm cultivated under mixotrophic growth conditions

2017 ◽  
Vol 26 ◽  
pp. 153-160 ◽  
Author(s):  
Sandra Milena Rincon ◽  
Hernan Mauricio Romero ◽  
Wrya Moh Aframehr ◽  
Haluk Beyenal
Keyword(s):  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Growth Conditions ◽  
Mixotrophic Growth

Influence of air flow rate and backwashing on the hydraulic behaviour of a submerged filter

2013 ◽  
Vol 68 (9) ◽  
pp. 2000-2006 ◽  
Author(s):  
Yazmin Lucero Cobos-Becerra ◽  
Simón González-Martínez
Keyword(s):  
New Media ◽  
Air Flow ◽  
Pilot Scale ◽  
Void Volume ◽  
Dead Volume ◽  
Bulk Liquid ◽  
Air Bubbles ◽  
Air Flow Rate ◽  
Filter Media ◽  
Hydraulic Behaviour

The aim of this study was to evaluate backwashing effects on the apparent porosity of the filter media and on the hydraulic behaviour of a pilot scale submerged filter, prior to biofilm colonization, under different hydraulic retention times, and different air flow rates. Tracer curves were analysed with two mathematical models for ideal and non-ideal flow (axial dispersion and Wolf and Resnick models). The filter media was lava stones sieved to 4.5 mm. Backwashing causes attrition of media particles, decreasing the void volume of the filter media and, consequently, the tracer flow is more uniform. The eroded media presented lower dead volumes (79% for the filter with aeration and 8% for the filter without aeration) compared with the new media (83% for the filter with aeration and 22% for the filter without aeration). The flow patterns of eroded and new media were different because the more regular shape of the particles decreases the void volume of the filter media. The dead volume is attributed, in the case of the filter with aeration, to the turbulence caused by the air bubbles that generate preferential channelling of the bulk liquid along the filter media, creating large zones of stagnant liquid and, for the filter without aeration, to the channels formed due to the irregular shaped media.

scholarly journals Increased lipids production of Nannochloropsis oculata and Chlorella vulgaris for biodiesel synthesis through the optimization of growth medium composition arrangement by using bicarbonate addition

2018 ◽  
Vol 154 ◽  
pp. 01009
Author(s):  
Dianursanti ◽  
Zenitha Lintang Agustin ◽  
Dwini Normayulisa Putri
Keyword(s):  
Growth Medium ◽  
Chlorella Vulgaris ◽  
Inorganic Carbon ◽  
Pilot Scale ◽  
Nannochloropsis Oculata ◽  
Medium Composition ◽  
Biodiesel Synthesis ◽  
Key Factor ◽  
Inorganic Carbon Source ◽  
Lipids Production

Chlorella vulgaris and Nannochloropsis oculata are a highly potential microalgae to be used in pilot-scale of biodiesel synthesis. The essential content from these microalgae is the fatty acid of lipid which is the main target for the feed and biodiesel industries. One of the key factor in improving lipid microalgae are the arrangemment of nutrients in the growth medium. Research on the regulation of nutrients using bicarbonate (HCO3-) as an additional inorganic carbon source has been done by many studies, but the yield of lipids obtained has not been much. The aim of the study was to improve the lipid yield of Chlorella vulgaris and Nannochloropsis oculata. Variation of [HCO3-] which added to Walne medium were 25 ppm and 75 ppm, while the Walne medium without the addition of bicarbonate acts as control. The results showed that [HCO3-] 75 ppm could increase Chlorella vulgaris biomass by 0.9162 g/l with 17.0% wt, while Nannochloropsis oculata produced the greatest lipid content in [HCO3-] 25 ppm of 20.3% wt and the largest biomass on [HCO3-] 75 ppm of 1.7233 g/l.

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