Bitterness-masking peptides for epigallocatechin gallate identified through peptide array analysis

2021 ◽  
Vol 27 (2) ◽  
pp. 221-228
Author(s):  
Keisuke Ito ◽  
Mayu Koike ◽  
Yuki Kuroda ◽  
Toyomi Yamazaki-Ito ◽  
Yuko Terada ◽  
...  
Keyword(s):  
Epigallocatechin Gallate ◽  
Peptide Array ◽  
Array Analysis ◽  
Bitterness Masking

The B-cell epitope of the monoclonal anti-factor VIII antibody ESH8 characterized by peptide array analysis

2008 ◽  
Vol 99 (03) ◽  
pp. 634-637 ◽  
Author(s):  
Thilo Albert ◽  
Christian Egler ◽  
Stanislav Jakuschev ◽  
Ulrike Schuldenzucker ◽  
Alexandra Schmitt ◽  
...  
Keyword(s):  
Factor Viii ◽  
B Cell ◽  
Cell Epitope ◽  
Peptide Array ◽  
Array Analysis ◽  
B Cell Epitope

scholarly journals Insights into High Affinity Small Ubiquitin-like Modifier (SUMO) Recognition by SUMO-interacting Motifs (SIMs) Revealed by a Combination of NMR and Peptide Array Analysis

2011 ◽  
Vol 287 (5) ◽  
pp. 3231-3240 ◽  
Author(s):  
Andrew T. Namanja ◽  
Yi-Jia Li ◽  
Yang Su ◽  
Steven Wong ◽  
Jingjun Lu ◽  
...  
Keyword(s):  
Peptide Array ◽  
Array Analysis ◽  
High Affinity

scholarly journals Protein and Peptide Array Analysis of Autoimmune Disease

BioTechniques ◽  
2002 ◽  
Vol 33 (6S) ◽  
pp. S66-S69 ◽  
Author(s):  
William H. Robinson ◽  
Lawrence Steinman ◽  
Paul J. Utz
Keyword(s):  
Autoimmune Disease ◽  
Peptide Array ◽  
Array Analysis

Green tea catechin epigallocatechin gallate causes apoptosis and shows potential synergism with cisplatin in biliary tract cancer cells

2015 ◽  
Vol 53 (05) ◽  
Author(s):  
C Mayr ◽  
A Wagner ◽  
D Neureiter ◽  
M Pichler ◽  
M Ritter ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Green Tea ◽  
Biliary Tract ◽  
Biliary Tract Cancer ◽  
Epigallocatechin Gallate ◽  
Tract Cancer ◽  
Green Tea Catechin

Search for Compounds Suppressing Intestinal α-Glucosidase Expression in Caco-2 Cells

2019 ◽  
Vol 2 (2) ◽  
pp. 96-101
Author(s):  
Kota Noda ◽  
Eisuke Kato ◽  
Jun Kawabata
Keyword(s):  
Blood Glucose ◽  
Intestinal Tract ◽  
Cell Model ◽  
Epigallocatechin Gallate ◽  
Calluna Vulgaris ◽  
Active Principle ◽  
Intestinal Epithelial ◽  
Mrna Expression Level ◽  
Epicatechin Gallate ◽  
Control Post

Diabetes is a chronic disease characterized by elevated blood glucose level.Reducing carbohydrate absorption from the intestinal tract is an effective strategy to control post-meal blood glucose level. Inhibition of intestinal α-glucosidase, involved in digestion of carbohydrates, is known as an approach to accomplish this. On the other hand, reduction of α-glucosidase amount is expected to work in the similar manner. However, none of the previousstudy pursues this approach. A convenient assay was developed to evaluate α-glucosidase amount employing Caco-2 cells, the intestinal epithelial cell model reported to express α-glucosidase. Sixty plants were screened and two candidate plants, Calluna vulgaris and Perilla frutescens var. crispa were found to reduce α-glucosidase expression. C. vulgaris extract was subjected to activity guided isolation. Proanthocyanidin was identified as the active principle which was analyzed by thiol decomposition to reveal the components as a mixture ofcatechin, epicatechin, epigallocatechin, and A type procyanidin dimer. The proanthocyanidin suppressed about 30% of α-glucosidase amount evaluated through convenient assay, and suppressed bulk of mRNA expression level of sucrase-isomaltase (SI) at 0.125 mg/mL. Several flavan-3-ol monomers were also tested, and epicatechin gallate and epigallocatechin gallate were found to suppress α-glucosidase amount significantly.

CGH array analysis of pituitary tumors revealed a highly-disrupted genome

2018 ◽  
Author(s):  
Helene Lasolle ◽  
Mad-Helenie Elsensohn ◽  
Eudeline Alix ◽  
Clement Bonnefille ◽  
Jessica Michel ◽  
...  
Keyword(s):  
Pituitary Tumors ◽  
Cgh Array ◽  
Array Analysis

Faculty Opinions recommendation of eSGA: E. coli synthetic genetic array analysis.

2008 ◽  
Author(s):  
Mohan Balasubramanian ◽  
Mithilesh Mishra ◽  
Ramanujam Srinivasan
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis ◽  
E Coli

Isolation of Epigallocatechin Gallate from Green Tea and its Effects on Probiotics and Pathogenic Bacteria

2017 ◽  
Vol 17 (1) ◽  
pp. 69-77
Author(s):  
Tu Lijun ◽  
Sun Hanju ◽  
He Shudong ◽  
Zhu Yongsheng ◽  
Yu Ming ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Green Tea ◽  
Lactobacillus Acidophilus ◽  
Pathogenic Bacteria ◽  
Epigallocatechin Gallate ◽  
Microbial Populations ◽  
Medium Ph ◽  
Bioactive Substance ◽  
Tea Extract

The aim of this study was to investigate epigallocatechin gallate (EGCG) prebiotics activities systematically which was reported as a bioactive substance. Therefore, EGCG was separated by water extraction, resin purification and prep-HPLC. Then the production of EGCG was confirmed by HPLC and mass spectrometry (MS) analysis and its purify was 97.23%. EGCG extractive and green tea extract (GTE) were further incubated with Bifidobacterium infantis, B. adolescentis, B. bifidum and Lactobacillus acidophilus to study its effect on microbial populations and medium pH. Finally, Escherichia coli, Salmonella, Staphylococcus aureus and Candida albicans were employed as pathogenic bacteria to explore the antimicrobial activity of EGCG and GTE. The results demonstrated that EGCG extractive could be beneficial for the proliferation of Bifidobacterium and L. acidophilus and also inhibit some pathogenic bacteria. In conclusion, both EGCG extractive and GTE had prebiotics activities and the effects of EGCG extractive were superior to those of GTE.

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