scholarly journals Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions

2011 ◽  
Vol 12 (4) ◽  
Author(s):  
Junaid Muneer Raja ◽  
Nurina Anuar ◽  
And Badarulhisam Abdul Rahman
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Downstream Processing ◽  
Western World ◽  
Human Consumption ◽  
Cell Culture Medium ◽  
Serum Free ◽  
Ns0 Cells

Colorectal cancer is the third most common form of cancer and the second leading cause of cancer-related death in the Western world. The answers to such life threatening diseases and cancers are monoclonal antibodies (MAb's) which are widely used as therapeutic agents. World demand for currently approved MAb's is on the order of a few kilograms per year. However, new therapeutic MAb's are under development and require doses of several hundred milligrams to a gram over the course of therapy. Very often to cater for the special requirements for the growth of mammalian cells, serum is added to the cell culture medium. However, removal of serum from the cell culture medium is often carried out, especially if the end product is to be used for human consumption, in order to eliminate various disadvantages such as high physiological variability, high batch to batch variability, risk of contamination and high cost, and challenges posed in the downstream processing of the product. In this paper, the adaptation of cholesterol requiring NS0 cells to commercially available serum free media is presented. ABSTRAK: Kanser kolorektum merupakan kanser ketiga paling umum dan kini berada di tempat kedua penyebab kematian berkaitan kanser di negara Barat. Jawapan kepada penyakit yang mengancam nyawa dan penyakit kanser adalah antibodi monoklon (monoclonal antibodies ((MAb's)) yang digunakan sebagai agen terapeutik. Permintaan dunia terhadap MAb's yang diluluskan adalah dalam bilangan beberapa kilogram setahun. Namun, terapeutik MAb's yang baru adalah di bawah penyelidikan dan memerlukan beberapa ratus dos milligram hingga satu gram dalam satu peringkat terapi. Sering kali untuk memenuhi permintaan terhadap tumbesaran sel mamalia, serum dicampurkan dengan sel kultur perantara. Walaupun begitu, pemindahan serum dari sel kultur perantara sering dilakukan, terutamanya jika produk akhir digunakan untuk kegunaan manusia; untuk mengurangkan pelbagai kelemahan seperti kebolehubahan psikologi yang tinggi, kebolehubahan yang tinggi daripada satu kumpulan ke satu kumpulan lain, risiko pencemaran, kos yang tinggi, dan cabaran mendatang dalam pemprosesan produk. Dalam perbentangan ini, kolestrol yang diubah memerlukan sel NS0 yang dikomersilkan dengan serum bebas perantara.

Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium

2007 ◽  
Vol 40 (1-2) ◽  
pp. 98-103 ◽  
Author(s):  
Megha S. Even ◽  
Chad B. Sandusky ◽  
Neal D. Barnard ◽  
Jehangir Mistry ◽  
Madhur K. Sinha
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Human Insulin ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free

Preliminary clinical trials of serum free cell culture medium as a treatment for chronic atrophic ulcers

1997 ◽  
Vol 20 (3) ◽  
pp. 115-120 ◽  
Author(s):  
E. Lindenbaum ◽  
Y. Har-Shai ◽  
Y. Ullmann ◽  
A. L. Feitelberg ◽  
M. Tendler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Clinical Trials ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free

scholarly journals Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Miaomiao Chen ◽  
Xiaoshuang Zuo ◽  
Qinqin Xu ◽  
Rong Wang ◽  
Suhua Fan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Biomedical Applications ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Nanodiamonds (NDs) have been recognized as emerging carbon-based delivery vehicles due to their biocompatibility. NDs were reported to be nontoxic and suited for biomedical applications in the complete cell culture medium. However, in this study, the cytotoxicity of NDs in serum-free medium was studied and indicated that serum proteins in cell culture medium played significant effect on the toxicity of NDs. Therefore, the interaction mechanism between NDs and a serum protein (human serum albumin, HSA) was first investigated by fluorescence quenching technique and circular dichroism (CD) spectrometry. The results suggest that HSA strongly bonds on the NDs surface to form “protein corona,” which not only prevents the aggregation of NDs and improves its stability but also inhibits the cytotoxicity of NDs. In serum-free medium, NDs exhibited obvious toxicity toward the human lung epithelial cell line (BEAS-2B) and showed concentration-dependent cytotoxicity. In the presence of bovine serum albumin (BSA), which shares structural homology and similar properties with HSA, toxicity of NDs was apparently inhibited. Therefore, the interaction between serum protein and NDs should be considered in the understanding of the biological effects of NDs exposure in biomedical applications.

GTSF-2: A new, versatile cell culture medium for diverse normal and transformed mammalian cells

1997 ◽  
Vol 33 (5) ◽  
pp. 344-351 ◽  
Author(s):  
Peter I. Lelkes ◽  
Esther Ramos ◽  
Victor V. Nikolaychik ◽  
Dawn M. Wankowski ◽  
Brian R. Unsworth ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Cell Culture Medium

The Use of Artemia Salina in Toxicity Testing

1992 ◽  
Vol 20 (2) ◽  
pp. 297-301 ◽  
Author(s):  
Lillemor Lewan ◽  
Marianne Andersson ◽  
Paloma Morales-Gomez
Keyword(s):  
Cell Culture ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Cultured Cells ◽  
Sea Water ◽  
Toxicity Testing ◽  
Artemia Salina ◽  
Good Survival ◽  
Cell Culture Medium ◽  
A Cell

This study shows that the Artemia assay, which is usually performed by incubation for a 24-hour period in artificial sea water, can also be performed in phosphate buffered saline (PBS) at pH 7.2, or in a cell culture medium, both of which are used in toxicity assays employing mammalian cells. Thus, by using the equivalent media for incubation, toxic effects in the Artemia assay and toxic mechanisms can more accurately be compared with results obtained in mammalian cell toxicity. Survival of control animals is good for 72 hours, provided that infection can be avoided. A pH greater than 6 is essential for good survival of Artemia Salina, and a pH greater than 10.5 should be avoided. Because of the risk of infection at low saline concentrations, a decreased incubation time of 16 or 12 hours is recommended. The lethal concentrations (LC10 and LC50) in the 24-hour Artemia assay of the first ten chemicals in the MEIC programme were measured in PBS, and the results compared with those from a previous study of the effects in a 10-minute acute ATP leakage assay, specifically indicating lesions in the cell membrane. The EC10 and EC50 values for the alcohols in the Artemia assay were 35–75% of the corresponding values in the ATP leakage assay. For paracetamol and amitriptyline, the EC10 and EC50 values in the Artemia assay were 2–16% of the corresponding values in the ATP-leakage assay. The greatly increased toxicity of the two compounds in the animals may be related to systemic effects. FeSO4 was very toxic to Artemia salina at a concentration of lug/ml, but it did not cause leakage of ATP from cultured cells, even at a concentration of 8,000μg/ml, showing that widely different mechanisms of interaction with FeSO4 are measured by the two assays. A lower toxicity of polygodial, a sesquiterpenoid unsaturated dialdehyde, in cell culture medium, was obvious in the Artemia assay. Thus, some factors in cell culture medium must, by interacting with the toxic molecule, protect the animals against the toxicity of polygodial, as was previously found in cultured cells.

scholarly journals Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 466
Author(s):  
Stefano Panella ◽  
Francesco Muoio ◽  
Valentin Jossen ◽  
Yves Harder ◽  
Regine Eibl-Schindler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Culture Medium ◽  
Ex Vivo ◽  
Free Cell ◽  
Adipose Stem Cells ◽  
Cell Culture Medium ◽  
Serum Free ◽  
A Cell ◽  
Human Adipose Stem Cells

Adipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell drug” that is safe, reproducible, and cost-effective. Thus, we developed our own basal defined xeno- and serum-free cell culture medium (UrSuppe), specifically formulated to grow human adipose stem cells (hASCs). With this medium, we can directly culture the stromal vascular fraction (SVF) cells in defined cell culture conditions to obtain hASCs. Cells proliferate while remaining undifferentiated, as shown by Flow Cytometry (FACS), Quantitative Reverse Transcription PCR (RT-qPCR) assays, and their secretion products. Using the UrSuppe cell culture medium, maximum cell densities between 0.51 and 0.80 × 105 cells/cm2 (=2.55–4.00 × 105 cells/mL) were obtained. As the expansion of hASCs represents only the first step in a cell therapeutic protocol or further basic research studies, we formulated two chemically defined media to differentiate the expanded hASCs in white or beige/brown adipocytes. These new media could help translate research projects into the clinical application of hASCs and study ex vivo the biology in healthy and dysfunctional states of adipocytes and their precursors. Following the cell culture system developers’ practice and obvious reasons related to the formulas’ patentability, the defined media’s composition will not be disclosed in this study.

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