scholarly journals Burkholderia Pseudomallei Short-Chain Dehydrogenase/ Oxidoreductase: Potential Urine Biomarker Candidate for Acute Melioidosis

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
Aniza Pakeer ◽  
Vanitha Mariappan ◽  
How Soon Hin ◽  
Mohammed Imad A. Mustafa Mahmud
Keyword(s):  
Burkholderia Pseudomallei ◽  
Host Cells ◽  
Urine Samples ◽  
Short Chain ◽  
Tertiary Referral Center ◽  
Bacterial Proteins ◽  
Center Hospital ◽  
Urine Biomarker ◽  
Hydroxyacyl Coa Dehydrogenase ◽  
Short Chain Dehydrogenase

INTRODUCTION: In the current climate of urgency in identifying biomarkers for the development of rapid diagnostic kits, the use of urine samples to diagnose acute melioidosis was evaluated, comparing urine samples from Burkholderia pseudomallei culture-positive and culture-negative patients, and comparing pneumonic and septicemic melioidosis. MATERIAL AND METHODS: Eleven urine samples from clinically suspected melioidosis patients from a tertiary referral center, Hospital Tengku Ampuan Afzan, Pahang was used. An in-solution method for the detection of bacterial proteins using liquid chromatography-mass spectrometry quadrupole time-of-flight (LCMS QTOF) was used. RESULTS: Three bacterial proteins were consistently detected among all the culture[1]positive and PCR-positive cases tested, namely SDR family NAD(P)-dependent oxidoreductase protein (32kDa), 3-hydroxyacyl-CoA dehydrogenase Burkholderia sp. (32kDa), and NAD(P)-dependent dehydrogenase (short-subunit alcohol dehydrogenase family) Burkholderia sp. (33kDa). CONCLUSIONS: Short-chain dehydrogenase (SDO) proteins could potentially be a urine biomarker candidate as these have shown to aid in the ability of Burkholderia spp. to invade host cells as this action is important for the initial intracellular survival of the organism.

scholarly journals Altered proteome of a Burkholderia pseudomallei mutant defective in short-chain dehydrogenase affects cell adhesion, biofilm formation and heat stress tolerance

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8659 ◽  
Author(s):  
Onrapak Reamtong ◽  
Nitaya Indrawattana ◽  
Amporn Rungruengkitkun ◽  
Tipparat Thiangtrongjit ◽  
Taksaon Duangurai ◽  
...  
Keyword(s):  
Heat Stress ◽  
Biofilm Formation ◽  
Burkholderia Pseudomallei ◽  
Deletion Mutant ◽  
Stress Conditions ◽  
Host Cells ◽  
Short Chain ◽  
Wild Type ◽  
Short Chain Dehydrogenase

Burkholderia pseudomallei is a Gram-negative bacillus that causes melioidosis and is recognized as an important public health problem in southeast Asia and northeast Australia. The treatment of B. pseudomallei infection is hampered by resistance to a wide range of antimicrobial agents and no vaccine is currently available. At present, the underlying mechanisms of B. pseudomallei pathogenesis are poorly understood. In our previous study, we reported that a B. pseudomallei short-chain dehydrogenase (SDO; BPSS2242) mutant constructed by deletion mutagenesis showed reduced B. pseudomallei invasion and initial intracellular survival. This indicated that SDO is associated with the pathogenesis of melioidosis. In the present study, the role of B. pseudomallei SDO was further investigated using the SDO deletion mutant by a proteomic approach. The protein profiles of the SDO mutant and wild-type K96243 were investigated through gel-based proteomic analysis. Quantitative intensity analysis of three individual cultures of the B. pseudomallei SDO mutant revealed significant down-regulation of five protein spots compared with the wild-type. Q-TOF MS/MS identified the protein spots as a glutamate/aspartate ABC transporter, prolyl-tRNA synthetase, Hsp70 family protein, quinone oxidoreductase and a putative carboxypeptidase. Functional assays were performed to investigate the role of these differentially expressed proteins in adhesion to host cells, biofilm induction and survival under heat stress conditions. The SDO deletion mutant showed a decreased ability to adhere to host cells. Moreover, biofilm formation and the survival rate of bacteria under heat stress conditions were also reduced in the mutant strain. Our findings provide insight into the role of SDO in the survival and pathogenesis of B. pseudomallei at the molecular level, which may be applied to the prevention and control of B. pseudomallei infection.

scholarly journals Biochemical properties, tissue expression, and gene structure of a short chain dehydrogenase/reductase able to catalyze cis-retinol oxidation

1999 ◽  
Vol 40 (12) ◽  
pp. 2279-2292 ◽  
Author(s):  
Mary V. Gamble ◽  
Enyuan Shang ◽  
Roseann Piantedosi Zott ◽  
James R. Mertz ◽  
Debra J. Wolgemuth ◽  
...  
Keyword(s):  
Gene Structure ◽  
Tissue Expression ◽  
Biochemical Properties ◽  
Short Chain ◽  
Short Chain Dehydrogenase

Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice

2021 ◽  
Vol 348 ◽  
pp. 129046
Author(s):  
Mengyang Xing ◽  
Yong Chen ◽  
Boqiang Li ◽  
Shiping Tian
Keyword(s):  
Apple Juice ◽  
Short Chain ◽  
Short Chain Dehydrogenase

scholarly journals The Burkholderia pseudomallei intracellular ‘TRANSITome’

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yun Heacock-Kang ◽  
Ian A. McMillan ◽  
Michael H. Norris ◽  
Zhenxin Sun ◽  
Jan Zarzycki-Siek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Population Dynamics ◽  
Burkholderia Pseudomallei ◽  
Host Cells ◽  
Hypothetical Proteins ◽  
Membrane Protrusion ◽  
Complex Environments ◽  
Cell Infection ◽  
Prokaryotic Cell ◽  
Cell Spread

AbstractProkaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations, which has hampered further understanding of spatiotemporal and stage-specific processes of prokaryotic cells within complex environments. Here we develop a ‘TRANSITomic’ approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection at defined stages, yielding pathophysiological insights. We find that B. pseudomallei transits through host cells during infection in three observable stages: vacuole entry; cytoplasmic escape and replication; and membrane protrusion, promoting cell-to-cell spread. The B. pseudomallei ‘TRANSITome’ reveals dynamic gene-expression flux during transit in host cells and identifies genes that are required for pathogenesis. We find several hypothetical proteins and assign them to virulence mechanisms, including attachment, cytoskeletal modulation, and autophagy evasion. The B. pseudomallei ‘TRANSITome’ provides prokaryotic single-cell transcriptomics information enabling high-resolution understanding of host-pathogen interactions.

The VEP1 gene (At4g24220) encodes a short-chain dehydrogenase/reductase with 3-oxo-Δ4,5-steroid 5β-reductase activity in Arabidopsis thaliana L.

Biochimie ◽  
2009 ◽  
Vol 91 (4) ◽  
pp. 517-525 ◽  
Author(s):  
V. Herl ◽  
G. Fischer ◽  
V.A. Reva ◽  
M. Stiebritz ◽  
Y.A. Muller ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Reductase Activity ◽  
Short Chain ◽  
Short Chain Dehydrogenase
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