scholarly journals MEASUREMENT OF IN-SITU FLOW VELOCITY USING SINGLE-MOLECULE DETECTION FOR THE APPLICATION OF BIOMOLECULE QUANTIFICATION

2004 ◽  
Author(s):  
S.-Y. Chao ◽  
C.Y. Zhang ◽  
T.-H. Wang
Keyword(s):  
Flow Velocity ◽  
Single Molecule ◽  
Single Molecule Detection

In-situ hybridization and single-molecule detection

2006 ◽  
Vol 135 (1) ◽  
pp. 151-152 ◽  
Author(s):  
E. Milhiet ◽  
A. Débarre ◽  
P. Tchénio ◽  
J. Neveu ◽  
D. Comas ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Single Molecule ◽  
Single Molecule Detection

In situ single molecule detection of insulin receptors on erythrocytes from a type 1 diabetes ketoacidosis patient by atomic force microscopy

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7407-7416 ◽  
Author(s):  
Lu Zhang ◽  
Jiang Pi ◽  
Qiping Shi ◽  
Jiye Cai ◽  
Peihui Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Type 1 Diabetes ◽  
Single Molecule ◽  
Insulin Receptors ◽  
Single Molecule Detection ◽  
Force Microscopy ◽  
Atomic Force ◽  
Molecule Interactions

A method to investigate the single molecule interactions between insulin and insulin receptor in erythrocytes from healthy volunteer and type 1 diabetes ketoacidosis (T1-DKA) patient was introduced using atomic force microscopy (AFM).

scholarly journals Fusion FISH Imaging: Single-Molecule Detection of Gene Fusion Transcripts In Situ

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e93488 ◽  
Author(s):  
Fatu Badiane Markey ◽  
William Ruezinsky ◽  
Sanjay Tyagi ◽  
Mona Batish
Keyword(s):  
Single Molecule ◽  
Gene Fusion ◽  
Single Molecule Detection ◽  
Fusion Transcripts

Single Molecule Detection of Fluorescently Labelled DNA Dendrimers

2000 ◽  
Vol 6 (S2) ◽  
pp. 856-857
Author(s):  
TW Nilsen ◽  
R. Getts ◽  
M. Weinstein
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Microscopy ◽  
Western Blot ◽  
Single Molecule ◽  
Point Sources ◽  
Nucleic Acid Hybridization ◽  
Single Molecule Detection ◽  
Underlying Principle ◽  
Microscopic Techniques

Single molecule detection has been achieved via many highly sophisticated microscopic techniques. Here we describe the detection of single molecules with conventional epifluorescent microscopy. The key to the technique is the use of DNA dendrimers DNA dendrimers have demonstrated utility in nucleic acid blots, Southerns, Northerns, etc. Typically DNA dendrimers yield 50-100 fold gain in signal over comparably labeled oligonucleotides. Immunodendrimers, DNA dendrimers conjugated to antibody molecules, have also been constructed and utilized in western blot assays. Individual, i.e. single molecule, 4- layer dendrimers, are readily detectable as point sources via conventional fluorescence microscopy and are useful for in situ hybridization and flow fluorescence quantitation.Nucleic acid hybridization is the underlying principle behind DNA dendrimer assembly. The “monomer” of DNA dendrimers consists of partially double stranded heteroduplexed DNA. Each monomer has an approximately 50 base double stranded “waist” surrounded by four approximately 30 base single stranded “arms”.

scholarly journals A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases

2021 ◽  
Author(s):  
Li-juan Wang ◽  
Le Liang ◽  
Bing-jie Liu ◽  
BingHua Jiang ◽  
Chun-yang Zhang
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Amplification Cascade

A controlled T7 transcription-driven symmetric amplification cascade machinery is developed for single-molecule detection of multiple repair glycosylases.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

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