In Situ Single Molecule Detection on Cell Membrane and Label Molecule Distributions Using AFM/NSOM

2018 ◽  
pp. 41-54
Author(s):  
Jiang Pi ◽  
Hua Jin ◽  
Jiye Cai
Keyword(s):  
Cell Membrane ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Label Molecule

In-situ hybridization and single-molecule detection

2006 ◽  
Vol 135 (1) ◽  
pp. 151-152 ◽  
Author(s):  
E. Milhiet ◽  
A. Débarre ◽  
P. Tchénio ◽  
J. Neveu ◽  
D. Comas ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Single Molecule ◽  
Single Molecule Detection

In situ single molecule detection of insulin receptors on erythrocytes from a type 1 diabetes ketoacidosis patient by atomic force microscopy

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7407-7416 ◽  
Author(s):  
Lu Zhang ◽  
Jiang Pi ◽  
Qiping Shi ◽  
Jiye Cai ◽  
Peihui Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Type 1 Diabetes ◽  
Single Molecule ◽  
Insulin Receptors ◽  
Single Molecule Detection ◽  
Force Microscopy ◽  
Atomic Force ◽  
Molecule Interactions

A method to investigate the single molecule interactions between insulin and insulin receptor in erythrocytes from healthy volunteer and type 1 diabetes ketoacidosis (T1-DKA) patient was introduced using atomic force microscopy (AFM).

scholarly journals Fusion FISH Imaging: Single-Molecule Detection of Gene Fusion Transcripts In Situ

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e93488 ◽  
Author(s):  
Fatu Badiane Markey ◽  
William Ruezinsky ◽  
Sanjay Tyagi ◽  
Mona Batish
Keyword(s):  
Single Molecule ◽  
Gene Fusion ◽  
Single Molecule Detection ◽  
Fusion Transcripts

Single Molecule Detection of Fluorescently Labelled DNA Dendrimers

2000 ◽  
Vol 6 (S2) ◽  
pp. 856-857
Author(s):  
TW Nilsen ◽  
R. Getts ◽  
M. Weinstein
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Microscopy ◽  
Western Blot ◽  
Single Molecule ◽  
Point Sources ◽  
Nucleic Acid Hybridization ◽  
Single Molecule Detection ◽  
Underlying Principle ◽  
Microscopic Techniques

Single molecule detection has been achieved via many highly sophisticated microscopic techniques. Here we describe the detection of single molecules with conventional epifluorescent microscopy. The key to the technique is the use of DNA dendrimers DNA dendrimers have demonstrated utility in nucleic acid blots, Southerns, Northerns, etc. Typically DNA dendrimers yield 50-100 fold gain in signal over comparably labeled oligonucleotides. Immunodendrimers, DNA dendrimers conjugated to antibody molecules, have also been constructed and utilized in western blot assays. Individual, i.e. single molecule, 4- layer dendrimers, are readily detectable as point sources via conventional fluorescence microscopy and are useful for in situ hybridization and flow fluorescence quantitation.Nucleic acid hybridization is the underlying principle behind DNA dendrimer assembly. The “monomer” of DNA dendrimers consists of partially double stranded heteroduplexed DNA. Each monomer has an approximately 50 base double stranded “waist” surrounded by four approximately 30 base single stranded “arms”.

In situ fluorescent profiling of living cell membrane proteins at a single-molecule level

2019 ◽  
Vol 55 (28) ◽  
pp. 4043-4046 ◽  
Author(s):  
Yuanyuan Fan ◽  
Lu Li ◽  
Meng Lu ◽  
Haibin Si ◽  
Bo Tang
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Single Molecule ◽  
Signal Amplification ◽  
Living Cells ◽  
Single Molecule Level ◽  
Visualization Analysis ◽  
Amplification Method ◽  
Cell Membrane Proteins

A signal amplification method is developed for visualization analysis of membrane proteins on living cells at a single-molecule level.

The role of (bio)surfactant sorption in promoting the bioavailability of nutrients localized at the solid-water interface

1999 ◽  
Vol 39 (7) ◽  
pp. 91-98 ◽  
Author(s):  
Ryan N. Jordan ◽  
Eric P. Nichols ◽  
Alfred B. Cunningham
Keyword(s):  
Mass Transfer ◽  
Cell Membrane ◽  
Substrate Concentration ◽  
Water Interface ◽  
Industrial Systems ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Surfactant Sorption

Bioavailability is herein defined as the accessibility of a substrate by a microorganism. Further, bioavailability is governed by (1) the substrate concentration that the cell membrane “sees,” (i.e., the “directly bioavailable” pool) as well as (2) the rate of mass transfer from potentially bioavailable (e.g., nonaqueous) phases to the directly bioavailable (e.g., aqueous) phase. Mechanisms by which sorbed (bio)surfactants influence these two processes are discussed. We propose the hypothesis that the sorption of (bio)surfactants at the solid-liquid interface is partially responsible for the increased bioavailability of surface-bound nutrients, and offer this as a basis for suggesting the development of engineered in-situ bioremediation technologies that take advantage of low (bio)surfactant concentrations. In addition, other industrial systems where bioavailability phenomena should be considered are addressed.

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