scholarly journals HLA-PHENOTYPE IN PATIENTS WITH GLOMERULONEPHRITIS WITH VARIOUS MORPHOLOGIC FORMS AND NEPHROTIC SYNDROM

2016 ◽  
pp. 36-44
Author(s):  
M. Kolesnyk ◽  
V. Driyanska ◽  
G. Drannik ◽  
O. Petrina ◽  
M. Velychko ◽  
...  
Keyword(s):  
Renal Failure ◽  
Prognostic Markers ◽  
Hla Antigens ◽  
Chronic Glomerulonephritis ◽  
Hormone Resistance ◽  
Disease Course ◽  
Etiologic Role ◽  
Healthy Donors ◽  
Hla Phenotype

In the work was determined the HLA-phenotype specificities in patients with different morphologic forms of chronic glomerulonephritis and nephrotic syndrome (CGN, NS) to define the additional predictors of a disease course. Materials and methods. There was studied the HLA-antigens distribution in the 264 CGN, NS patients and 350 healthy donors by typing the lymphocytes with the aid ofstandard microlymphocytotoxic test (Terasaki’s test). The diagnosis was confirmed morphologically using the thin needle nephrobiopsy. Results. It is advisable to associate CGN, NS (RR > 2) with antigens HLA- A23, 24, 28; B8, 38, 41, 44 in patients; the causal role (a > 0.1) was determined for A24, 28; B8. In proliferative GN was additionally revealed the etiologic role of B27 known as antigen associated with risk ofautoimmune diseases. In patients with various morphologic forms is advisable the association of some antigens with development of chronic renal failure (CRF) – A30, B41 in FSGS, A10 – MGN; and also hormone resistance (HR) – A19+31+32 in FSGS, B8 – MGN and MC. Conclusion. The revealed reliable associations ofHLA types both with CGN, NS and its separate morphologic forms with the risk of CRF and/or HR allow take into consideration the availability ofsuch antigens in phenotype ofpatients with confirmed by biopsy diagnosis as the additional diagnostic and prognostic markers.

scholarly journals ASSOCIATION OF HLA AND PROINFLAMMATORY CYTOKINES OF BLOOD IN PATIENTS WITH GLOMERULONEPHRITIS

2017 ◽  
pp. 35-41 ◽  
Author(s):  
M. Kolesnyk ◽  
V. Driyanska ◽  
M. Velychko ◽  
G. Drannik ◽  
O. Petrina
Keyword(s):  
Renal Failure ◽  
Nephrotic Syndrome ◽  
Proinflammatory Cytokines ◽  
Prognostic Markers ◽  
Hla Antigens ◽  
Serum Levels ◽  
High Serum ◽  
Chronic Glomerulonephritis ◽  
Healthy Donors ◽  
Negative Marker

Introduction. Cytokines and HLA are of important part of immunogenesis of many diseases, therefore the analysis of these indices and this associations in dependence of glomerulonephritis (GN) can define their value as the additional prognostic markers. Aim of the work is to determine the peculiarities of associations the high serum levels ofproinflammatory cytokines (TNF a, MCP-1, IL-18) and some HLA in phenotype to substantiate of chronic glomerulonephritis with nephrotic syndrome (CGN, NS) immunogenesis and to ascertain the additional prognostic markers. Materials and methods. There was studied the HLA-antigens distribution in the 264 CGN, NS adult patients and 350 healthy donors by typing the lymphocytes with the aid of standard microlymphocytotoxic test (Terasaki’s test). Using IFA, the level of the proinflammatory cytokines was studied in the blood serum - MCP-1 in 39, IL-18 – 40 and TNF-a - 96patients. Results. HLA-A23, -24, -B8, -38, -41, -44, DR1, -4, -w52 in adults patients have associations (RR>2) CGN, NS; the attributive risk (a>0,1) to develop GN detected in patients have A24, B8, DR 1, 4, w52. The relative risk (RR) to develop chronic renal failure (CRF) is in detection of HLA-10, -29, -30, -41, -51, DR4; attributive risk (AR) - A10. The CGN, NS patients showed statistically higher level of the serum proinflammatory cytokines – TNF-6, IL-17, MCP-1 with more high indices of the TNF-a in patients with HLA-A23, -A28, -B44 (RR of CGN, NS), -A10 (AR of CRF), IL-18 - A24 (AR of CGN, NS) ma A10 (AR of CRF). The highest levels of MCP-1 detected in adults case have risk antigens - relative B41, attributive - A28, B8, and predictor of CRF B41, wich may be negative marker for prognosis. Conclusion. It was to determine associations the serum levels of some cytokines and HLA in patients with CGN, NS. We think it appropriate to study HLA and proinflammatory cytokines TNF-a, IL-18 and MCP-1 in blood as additional negative prognostic predictors for the differentiating approach to treatment.

scholarly journals ASSOTIATIONS OF PECULIARITIES OF HLA-PHENOTYPE AND THE SENSIBILITY TO THE CORTICOSTEROID TREATMENT IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS WITH NEPHROTIC SYNDROME

2017 ◽  
pp. 37-44
Author(s):  
M. Kolesnyk ◽  
G. Drannik ◽  
V. Driyanska ◽  
O. Petrina ◽  
M. Velychko
Keyword(s):  
Nephrotic Syndrome ◽  
Hla Antigens ◽  
Corticosteroid Treatment ◽  
Steroid Resistance ◽  
Control Group ◽  
Chronic Glomerulonephritis ◽  
Healthy Donors ◽  
Steroid Sensitive ◽  
Steroid Sensitivity ◽  
Hla Phenotype

The purpose of study was determination of HLA -antigens I and II classes as predictors of ineffectiveness of initial steroid therapy, and according prognozonegative markers of chronic glomerulonephritis with nephrotic syndrome. Methods. In 59 chronic glomerulonephritis with nephrotic syndrome patients (steroid sensitive n=33 (1 gr.) and steroid resistant’s n= 26 (2 gr.)) and 350 healthy donors( control group) studied HLA antigens I and II classes of the special anti- HLA-antigens panel (20 antigens of locus A, 31 – of locus B and 9- of locus DR). Result. In patients with chronic glomerulonephritis, nephrotic syndrome with hormone sensitivity relative risk is high at the presents of A28 (RR=8,5, r р <0,001), it made attributive risk (=0,37). In comparison with a control group, RR>2 for antigens  A11  (RR=2,23), A23 (RR=4,28),  A24 (RR=3,3),  A29 (RR=10,78) that A30 (RR=11,23); attributive risk more than 0,1 for the antigen A11 (=0,16) ; A24 (=0,13), other did not differ from control. Subzero connection is exposed for the antigens of A2 (р<0,001), А9 (р=0,007). In locus antigen B14 (RR=5,65, р =0,001) are exposed, B44 (RR=48,25, р =0,004), B51(RR=12,32, р =0,006) and attributive risk of development of disease (according =0,24, 0,12 ; 0,14); and antigens B38 and B41 (RR=11,57, р=0,05). The steroid sensitivity was associated with the antigens B5 (p=0,033), B12 (p=0,005) and B35 (p=0,021). In locus DR made etiologic faction antigens DR4 (RR=7,0 and =0,24) DRw52 (RR=7,0 and =0,25). Conclusions. For patients with chronic glomerulonephritis with a nephrotic syndrome antigens of HLA-B14,B38, B51, DRw52 are associated with steroid sensitivity. The attributive risk of steroid resistance is high for split A19+31+32, antigens B8, B55.

scholarly journals ASSOCIATION OF HLA AND CYTOKINES OF BLOOD WITH ANTIINFLAMMATORY EFFECTS IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS

2017 ◽  
pp. 13-19
Author(s):  
M. Kolesnyk ◽  
V. Driyanska ◽  
G. Drannik ◽  
O. Petrina ◽  
M. Velychko ◽  
...  
Keyword(s):  
Serum Level ◽  
Prognostic Markers ◽  
Hla Antigens ◽  
Serum Levels ◽  
High Serum ◽  
Chronic Glomerulonephritis ◽  
Healthy Donors ◽  
Lower Serum Level ◽  
Steroid Sensitive ◽  
Antiinflammatory Effects

Cytokines and HLA take important part in immunogenesis of many diseases, therefore the analysis of these indices and this associations in dependence of glomerulonephritis (GN) can define their value as the additional prognostic markers. Aim of the work is to determine the of associations the high serum levels of cytokines (IL-4, IL-17) and peculiarities of some HLA in phenotype to substantiate of chronic glomerulonephritis with nephrotic syndrome (CGN, NS) immunogen- esis and to ascertain the additional prognostic markers. Materials and methods. There was studied the HLA-antigens distribution in the 264 adult patients CGN, NS (the diagnosis was confirmed morphologically using the thin needle nephrobiopsy) and 350 healthy donors by typing the lymphocytes with the aid of standard microlymphocytotoxic test (Terasaki’s test). HLA antigens were defined using a standard microlymphocytotoxic test on the Terasaki's planchette with special panels ofanti-HLA serums (20 antigens of locus A, 31 - B and 9 - DR). The etiologic fraction (attributive risks ) was counted using the formula: ct = x - y/I - y, where x - frequency of antigen in patients and y - frequency in healthy. The ct reading was considered significant when it exceeded 0,1. Using ELISA, the level of the cytokines was studied in the blood serum - IL-4 in 76 and IL-17 – 79 patients. Results. HLA-A23, -24, -28, -B8, -38, -41, -44, DR1, -4, -w52 in adults patients have associations (RR>2) CGN, NS; the attributive risk (AR) (<j>0,1) to develop GN detected in patients have A24, A28, B8, DR 1, 4, w52. The CGN, NS patients has statistically higher serum level of the IL-4 and IL-17, with more high indices of this cytokines in patients with attributive risk antigens HLA-A24 and A-28. The highest levels of IL-17 detected also in adults case have B14 and B38, which associated with steroid sensitive NS. HLA-B8, which associated with steroid resistant NS, have more patients with CGN, NS with lower serum level of antiinflammatory IL-4. Conclusion. The patients with CGN, NS have associations of HLA and serum levels of pro- and antiinflammatory cytokines IL-4 and IL-17, which play role of additional prognostic predictors.

scholarly journals Peculiarities of phenotypes of patients with pyelo- and glomerulonephritis by HLA distribution analysis

2018 ◽  
pp. 11-18
Author(s):  
V. Driianska ◽  
O. Petrina ◽  
M. Velychko ◽  
F. Haisenyuk ◽  
G. Drannik
Keyword(s):  
Kidney Diseases ◽  
Hla Antigens ◽  
Causal Role ◽  
Control Group ◽  
Distribution Analysis ◽  
Immune Disorder ◽  
Hla Antigen ◽  
Healthy Donors ◽  
Hla Phenotype ◽  
High Production

Studies devoted to the role of human leucocyte antigens (HLA) in pathogenesis of chronic kidney disease (CKD) have demonstrated the associative links of the HLA antigens, which stipulate the relative and attributive risks of some autoimmune diseases, with immune disorder and a high production of pro-inflammatory cytokines. The aim of our study was to determine the peculiarities of phenotypes of CKD patients according to the distribution of HLA-A, B and DR antigens and to conduct their comparative analysis in patients with pyelonephritis (PN) and glomerulonephritis (GN). Methods: The distribution of HLA-A, B, DR antigens in 384 CKD patients (120 with PN and 264 with GN) was analyzed. HLA antigens were defined using a standard microlymphocytotoxic test on the Terasakiґs planchette with special panels of anti-HLA serums (20 antigens of locus A, 31 – B and 9 – DR). The control group consisted of 350 healthy donors. The HLA antigen frequencies in normal and diseased subjects were compared taking each antigen separately, using χ2 test. The etiologic fraction (attributive risk s > 0,1) was counted using the formula: s = x - y/I- y, where x is frequency of antigen in patients and y is frequency in healthy. The s  reading was considered reliable when it exceeded 0.1. Results. The causal role (σ > 0,1) was determined for А10, А11; В14, В16 for PN; antigens-protectors - А2, В21, В35, В40. For CGN, NS the relative risk is high (RR > 2) at the presence of HLA-A23, А24, А28; B8, В38, В41, В44; DR1, DR4, DRw52 in phenotype, the causal role in etiopathology (σ>0.1) is indicated for A24,А28; B8; DR1, DR4, DRw52; the disease protectors are B12 and B16. Conclusion. Conclusion. The features of the HLA-phenotype of patients with pyelo- and glomerulonephritis were shown. It allowed to establish the interconnectedness of the antigens of the histocompatibility complex with the risk of kidney diseases developing, which could help to personificate of the treatment and predicte of the course of the disease.

scholarly journals VASCULAR ENDOTHELIAL GROWTH FACTOR AND HLA-PHENOTYPE IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS

2016 ◽  
pp. 16-21
Author(s):  
М. Kolesnyk ◽  
V. Driyanska ◽  
М. Velychko ◽  
G. Drannik ◽  
V. Nepomnyaschiy ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Hla Antigens ◽  
Serum Levels ◽  
High Serum ◽  
Chronic Glomerulonephritis ◽  
Vascular Endothelial ◽  
High Serum Level ◽  
Hla Phenotype ◽  
Endothelial Growth Factor

It was to determine associations the serum levels ofVEGF and with HLA inpatients with chronic glomerulonephritis and nephrotic syndrome (CGN, NS). Materials and methods. There was studied the serum levels of VEGF (80 patients) by ELIZA and HLA-antigens distribution in the CGN, NS patients (534) by typing the lymphocytes with the aid of standard microlymphocytotoxic test (Terasaki’s test). The diagnosis was confirmed morphologically using by nephrobiopsy. Results. The distribution of HLA-A, В antigens of the 2 group patients having the CGN, NS with various serum levels of VEGF is shown. Associations of some HLA-antigens and high levels of vascular endothelial growth factor in blood were noted. Conclusion. High serum level VEGF and HLA-A9, A10 (25+26) and B8 in CGN, NS patients associated with steroidoresistence.

scholarly journals NF-κB, JNK, and TLR Signaling Pathways in Hepatocarcinogenesis

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Shin Maeda
Keyword(s):  
Hepatocellular Carcinoma ◽  
Signaling Pathways ◽  
Inflammatory Cell ◽  
Prognostic Markers ◽  
Tumor Promotion ◽  
Neoplastic Cell ◽  
Nuclear Factor ◽  
Tlr Signaling ◽  
The Third

Hepatocellular carcinoma (HCC) is the third largest cause of cancer deaths worldwide. The role of molecular changes in HCC have been used to identify prognostic markers and chemopreventive or therapeutic targets. It seems that toll-like receptors (TLRs) as well as the nuclear factor (NF)-κB, and JNK pathways are critical regulators for the production of the cytokines associated with tumor promotion. The cross-talk between an inflammatory cell and a neoplastic cell, which is instigated by the activation of NF-κB and JNKs, is critical for tumor organization. JNKs also regulate cell proliferation and act as oncogenes, making them the main tumor-promoting protein kinases. TLRs play roles in cytokine and hepatomitogen expression mainly in myeloid cells and may promote liver tumorigenesis. A better understanding of these signaling pathways in the liver will help us understand the mechanism of hepatocarcinogenesis and provide a new therapeutic target for HCC.

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