Computed Tomography Evaluation of Urinary Stones Densities Compared to in Vitro Analysis of Its Chemical Composition

2019 ◽  
Author(s):  
Keyword(s):  
Computed Tomography ◽  
Chemical Composition ◽  
Urinary Stones ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals An in vitro analysis of the total phenolic content, antioxidant power, physical, physicochemical, and chemical composition of Terminalia Catappa Linn fruits

2012 ◽  
Vol 32 (1) ◽  
pp. 209-213 ◽  
Author(s):  
Marcelo Rodrigues Marques ◽  
Diego Damasceno Paz ◽  
Lívia Patrícia Rodrigues Batista ◽  
Celma de Oliveira Barbosa ◽  
Marcos Antônio Mota Araújo ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Average Weight ◽  
Antioxidant Power ◽  
Terminalia Catappa ◽  
Solids Content ◽  
Vitro Analysis ◽  
In Vitro Analysis

This study assessed the antioxidant, total phenolic, and physicochemical properties of in vitro Terminalia Catappa Linn (locally called castanhola) using the DPPH assay. The castanhola fruits had an average weight of 19.60 ± 0.00 g, combining shell, pulp, and seed weight, and a soluble solids content of 8 °Brix. The chemical composition was determined with predominance of carbohydrates (76,88 ± 0,58%).The titration method was used to determine Vitamin C content using 2,6-dichlorophenolindophenol (DCFI), known as reactive Tillmans resulting in no significant levels. Aqueous extracts of castanhola pulp showed a higher concentration of phenolics, 244.33 ± 18.86 GAE.g-1 of fruit, and alcoholic extracts, 142.84 ± 2.09 GAE.g-1 of fruit. EC50 values of the aqueous extract showed a greater ability to scavenge free radicals than the alcoholic extracts. The fruit had a significant content of phenolic compounds and high antioxidant capacity.

scholarly journals CT, US and MRI of Xanthine Urinary Stones: An In-Vitro Analysis

2020 ◽  
Author(s):  
Stephanie Shamir ◽  
Qi Peng ◽  
Alan Schoenfeld ◽  
Beth Drzewiecki ◽  
Mark Liszewski
Keyword(s):  
Energy Levels ◽  
Urinary Stones ◽  
Hounsfield Units ◽  
Ultrashort Echo Time ◽  
Imaging Characteristics ◽  
Magnetic Resonance Imaging Mri ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Acoustic Shadowing

Abstract Background: Xanthine urinary stones are a rare entity that may occur in patients with Lesch-Nyhan Syndrome receiving allopurinol. There is little literature describing imaging characteristics of these stones, and the most appropriate approach to imaging these stones is therefore unclear. We performed an in-vitro analysis of xanthine stones using computed tomography (CT) at different energy levels, ultrasound (US), and magnetic resonance imaging (MRI). Methods: Five pure xanthine stones from a boy with Lesch-Nyhan were imaged. CT of the stones was performed at 80kVp, 100kVp, 120kVp and 140kVp and CT numbers of the stones were recorded in Hounsfield units (HU). US of the stones was performed and echogenicity, acoustic shadowing and twinkle artifact were assessed. MRI of the stones was performed and included T2-weighted, ultrashort echo-time (UTE)-weighted and T2/T1-weighted 3D bFFE sequences and signal was assessed.Results: On CT, xanthine stones were radiodense and the average attenuation coefficient did not differ with varying kVp, measuring 331.0+/-51.7HU at 80kVp, 321.4+/-63.4HU at 100kVp, 329.7+/-54.2HU at 120kVp and 328.4+/-61.1HU at 140kVp. On US, xanthine stones where echogenic with acoustic shadowing and twinkle artifact. On MRI, stones lacked signal on all tested sequences.Conclusion: Xanthine stones demonstrate imaging characteristics typical of most urinary stones: dense on CT, echogenic on US, and lack signal on MRI. Therefore, the approach to imaging xanthine urinary stones should identical to the approach with other urinary stones.

scholarly journals In vitro analysis of dental ceramics: evaluation of the radiopacity and chemical composition by raman spectroscopy

2018 ◽  
Vol 21 (2) ◽  
pp. 164
Author(s):  
Rodrigo Furtado De Carvalho ◽  
Fernando Luiz Goulart Cruz ◽  
Debora Pinto Antunes ◽  
Evane Gonçalves Toledo Júnior ◽  
Luiz Fernando Cappa De Oliveira ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Chemical Composition ◽  
Analysis Of Variance ◽  
Dental Ceramics ◽  
Restorative Dentistry ◽  
Digital Radiographs ◽  
Vitro Analysis ◽  
Post Hoc ◽  
In Vitro Analysis

<p><strong>Objective</strong>: This study compared the radiopacity of different ceramic systems by means of digital radiographs and evaluate the chemical composition of the samples by Raman spectroscopy. <strong>Material and Methods</strong>: The hypothesis tested was that there was a difference in radiopacity among the tested materials. Specimens were prepared for each ceramic tested: FLD - VM7 (VITA Zahnfabrik), LD - IPS Empress e.max Press (IPS Empress), AL - In Ceram Alumina (VITA Zahnfabrik), ALYZ - In Ceram Zirconia (VITA Zahnfabrik), YZ - Lava All Ceram (3M/ESPE), and MYZ - Zirconzahn (Talladium Brazil). The specimens were radiographed and submitted to radiographic density readings using a histogram tool. The spectrometer coupled to a petrographic microscope was used for Raman spectroscopy measurements. Analysis of variance (ANOVA) and a Tukey post-hoc test were used to compare radiopacity of the different materials. <strong>Results</strong>: For all tested materials, the radiopacity showed statistically significant differences, except YZ and MYZ. Lava All Ceram and ZirkonZahn had high radiopacity values and VM7 and IPS Empress e.max Press showed lower radiopacity than human dental structures. <strong>Conclusion</strong>: It was possible to conclude that radiopacity is closely linked to ceramic chemical composition.</p><p><strong>Keywords</strong></p><p>Dental ceramics; Radiopacity; Raman spectroscopy test; Restorative dentistry.</p>

scholarly journals CT, US and MRI of Xanthine Urinary Stones: An In-Vitro Analysis

2020 ◽  
Author(s):  
Stephanie Shamir ◽  
Qi Peng ◽  
Alan Schoenfeld ◽  
Beth Drzewiecki ◽  
Mark Liszewski
Keyword(s):  
Energy Levels ◽  
Urinary Stones ◽  
Hounsfield Units ◽  
Ultrashort Echo Time ◽  
Imaging Characteristics ◽  
Magnetic Resonance Imaging Mri ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Acoustic Shadowing

Abstract Background: Xanthine urinary stones are a rare entity that may occur in patients with Lesch-Nyhan Syndrome receiving allopurinol. There is little literature describing imaging characteristics of these stones, and the most appropriate approach to imaging these stones is therefore unclear. We performed an in-vitro analysis of xanthine stones using computed tomography (CT) at different energy levels, ultrasound (US), and magnetic resonance imaging (MRI). Methods: Five pure xanthine stones from a boy with Lesch-Nyhan were imaged. CT of the stones was performed at 80kVp, 100kVp, 120kVp and 140kVp and CT numbers of the stones were recorded in Hounsfield units (HU). US of the stones was performed and echogenicity, acoustic shadowing and twinkle artifact were assessed. MRI of the stones was performed and included T2-weighted, ultrashort echo-time (UTE)-weighted and T2/T1-weighted 3D bFFE sequences and signal was assessed.Results: On CT, xanthine stones were radiodense and the average attenuation coefficient did not differ with varying kVp, measuring 331.0+/-51.7HU at 80kVp, 321.4+/-63.4HU at 100kVp, 329.7+/-54.2HU at 120kVp and 328.4+/-61.1HU at 140kVp. On US, xanthine stones where echogenic with acoustic shadowing and twinkle artifact. On MRI, stones lacked signal on all tested sequences.Conclusion: Xanthine stones demonstrate imaging characteristics typical of most urinary stones: dense on CT, echogenic on US, and lack signal on MRI. Therefore, the approach to imaging xanthine urinary stones should identical to the approach with other urinary stones.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

1163: Radial Dilation of Ureteral Balloons: A Comparative in-Vitro Analysis

2005 ◽  
Vol 173 (4S) ◽  
pp. 315-316
Author(s):  
Kari Hendlin ◽  
Brynn Lund ◽  
Manoj Monga
Keyword(s):  
Vitro Analysis ◽  
In Vitro Analysis
Sign in / Sign up

Export Citation Format

Share Document