scholarly journals An in vitro analysis of the total phenolic content, antioxidant power, physical, physicochemical, and chemical composition of Terminalia Catappa Linn fruits

2012 ◽  
Vol 32 (1) ◽  
pp. 209-213 ◽  
Author(s):  
Marcelo Rodrigues Marques ◽  
Diego Damasceno Paz ◽  
Lívia Patrícia Rodrigues Batista ◽  
Celma de Oliveira Barbosa ◽  
Marcos Antônio Mota Araújo ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Average Weight ◽  
Antioxidant Power ◽  
Terminalia Catappa ◽  
Solids Content ◽  
Vitro Analysis ◽  
In Vitro Analysis

This study assessed the antioxidant, total phenolic, and physicochemical properties of in vitro Terminalia Catappa Linn (locally called castanhola) using the DPPH assay. The castanhola fruits had an average weight of 19.60 ± 0.00 g, combining shell, pulp, and seed weight, and a soluble solids content of 8 °Brix. The chemical composition was determined with predominance of carbohydrates (76,88 ± 0,58%).The titration method was used to determine Vitamin C content using 2,6-dichlorophenolindophenol (DCFI), known as reactive Tillmans resulting in no significant levels. Aqueous extracts of castanhola pulp showed a higher concentration of phenolics, 244.33 ± 18.86 GAE.g-1 of fruit, and alcoholic extracts, 142.84 ± 2.09 GAE.g-1 of fruit. EC50 values of the aqueous extract showed a greater ability to scavenge free radicals than the alcoholic extracts. The fruit had a significant content of phenolic compounds and high antioxidant capacity.

scholarly journals Chemical Characterization and in Vitro Antioxidant Activity of Honey from Different Localities of Côte D’ivoire

2021 ◽  
pp. 22-34
Author(s):  
Kabran Guy Roger Mida ◽  
Yeboue Koffi Apollinaire ◽  
Adopo Sopie Sheilla Flavie ◽  
Mamyrbékova-Békro Janat Akhanovna ◽  
Békro Yves-Alain
Keyword(s):  
Chemical Composition ◽  
Secondary Metabolites ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Cote D’Ivoire ◽  
Antioxidant Power ◽  
Côte D'ivoire

Aims: Although the honey produced in Côte d'Ivoire is widely used in households, there is no technical sheet mentioning its chemical composition. Thus, the present study aimed to fill this gap while studying the chemical composition and antioxidant power of eleven honey samples from nine localities in Côte d'Ivoire. Methodology: The identification of secondary metabolites in the different honey samples was carried out by TLC and by color reaction tests. The quantification of these was performed by spectrophotometry. The antioxidant power of the honey samples was studied by DPPH and FRAP methods. Results: All honey samples contains secondary metabolites from various families, with varying contents of total phenolic compounds and flavonoids. Overall, the honey samples showed a notable antioxidant profile, making them good free radical scavengers. The relationship established between the different variables analyzed showed in some cases positive and significant correlations. Conclusion: The results of this study show that the honey produced in Côte d'Ivoire is of good quality. Therefore, its recurrent consumption could contribute to fighting certain pathologies caused by oxidative stress.

scholarly journals Physical–Chemical Composition and Quality Related Changes in “Ruaner” Pear (Pyrus ussuriensis) During Freezing–Thawing Period

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2611
Author(s):  
Yulian Liu ◽  
Yuxia Wu ◽  
Fei Che ◽  
Zhimin Zhang ◽  
Baihong Chen
Keyword(s):  
Chemical Composition ◽  
Water Content ◽  
Soluble Sugars ◽  
Total Flavonoid ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Soluble Solids Content ◽  
Physical Chemical ◽  
Postharvest Ripening ◽  
Solids Content

“Ruaner” pear (Pyrus ussuriensis Maxim.) is a fruit crop that is frequently served frozen in China. It is a typical postharvest ripening fruit that needs to ripen after harvest before it can be eaten, and freezing–thawing is one way that pears are treated during postharvest ripening. In order to study the physical–chemical composition and quality-related changes in “Ruaner” pears that result the freezing–thawing period, “Ruaner” pears were kept in a freezer (−20 °C) for 7 days, after which they were transferred to room temperature for thawing. The color of the peel of the “Ruaner” pears changed from yellow-green to yellow and then brown. The chlorophyll content and titratable acidity (TA) decreased significantly throughout 0–12 h period. The carotenoid content tended to rise and then decrease, peaking at 3 h after thawing (HAT), while the soluble solids content (SSC), firmness, total phenolic content, and total flavonoid content all generally decreased. The composition of soluble sugars and organic acids was examined in “Ruaner” pears, and the major soluble sugars were fructose and glucose, with citric acid being the most abundant organic acid. The data suggest that freezing–thawing significantly changes firmness, water content, SSC, and TA in “Ruaner” pears. At 3–4 HAT, “Ruaner” pears have moderate hardness, high water content, low acid content, and higher total phenolic, total flavonoid, and soluble solids content. Therefore, 3–4 HAT is the best time for pears in terms of both table and processing quality.

scholarly journals In vitro analysis of dental ceramics: evaluation of the radiopacity and chemical composition by raman spectroscopy

2018 ◽  
Vol 21 (2) ◽  
pp. 164
Author(s):  
Rodrigo Furtado De Carvalho ◽  
Fernando Luiz Goulart Cruz ◽  
Debora Pinto Antunes ◽  
Evane Gonçalves Toledo Júnior ◽  
Luiz Fernando Cappa De Oliveira ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Chemical Composition ◽  
Analysis Of Variance ◽  
Dental Ceramics ◽  
Restorative Dentistry ◽  
Digital Radiographs ◽  
Vitro Analysis ◽  
Post Hoc ◽  
In Vitro Analysis

<p><strong>Objective</strong>: This study compared the radiopacity of different ceramic systems by means of digital radiographs and evaluate the chemical composition of the samples by Raman spectroscopy. <strong>Material and Methods</strong>: The hypothesis tested was that there was a difference in radiopacity among the tested materials. Specimens were prepared for each ceramic tested: FLD - VM7 (VITA Zahnfabrik), LD - IPS Empress e.max Press (IPS Empress), AL - In Ceram Alumina (VITA Zahnfabrik), ALYZ - In Ceram Zirconia (VITA Zahnfabrik), YZ - Lava All Ceram (3M/ESPE), and MYZ - Zirconzahn (Talladium Brazil). The specimens were radiographed and submitted to radiographic density readings using a histogram tool. The spectrometer coupled to a petrographic microscope was used for Raman spectroscopy measurements. Analysis of variance (ANOVA) and a Tukey post-hoc test were used to compare radiopacity of the different materials. <strong>Results</strong>: For all tested materials, the radiopacity showed statistically significant differences, except YZ and MYZ. Lava All Ceram and ZirkonZahn had high radiopacity values and VM7 and IPS Empress e.max Press showed lower radiopacity than human dental structures. <strong>Conclusion</strong>: It was possible to conclude that radiopacity is closely linked to ceramic chemical composition.</p><p><strong>Keywords</strong></p><p>Dental ceramics; Radiopacity; Raman spectroscopy test; Restorative dentistry.</p>

scholarly journals Species-Specific Antioxidant Power and Bioactive Properties of the Extracts Obtained from Wild Mediterranean Calendula Spp. (Asteraceae)

2019 ◽  
Vol 9 (21) ◽  
pp. 4627 ◽  
Author(s):  
Concetta Maria Messina ◽  
Angelo Troia ◽  
Rosaria Arena ◽  
Simona Manuguerra ◽  
Theodora Ioannou ◽  
...  
Keyword(s):  
Supercritical Fluids ◽  
Reducing Power ◽  
Mediterranean Area ◽  
Antioxidant Power ◽  
High Antioxidant Activity ◽  
Species Specific ◽  
Vitro Analysis ◽  
Toxic Organic Solvents ◽  
In Vitro Analysis

In this study we focused on four taxa of the genus Calendula (C. maritima, C. suffruticosa subsp. fulgida, C. arvensis, and the hybrid between the first two ones), collected in Mediterranean area (Sicily). Six extracts for each species were obtained using solvents with increasing polarity (hexane, ethanol 80%, acetone 70%, and water) and through extraction by supercritical fluids (SFE). It has been observed that the solvent with the highest extraction efficiency was ethanol 80% for all species. However, SFE extracts showed high antioxidant activity comparable to the ethanol 80% extract (polyphenol, DPPH, and reducing power method). These findings were confirmed by in vitro analysis (MTT assay) where it was observed that the tested concentration (24 µg/mL), obtained from ethanol 80% and SFE extracts, showed a protective effect comparable to that induced by a synthetic antioxidant. Extraction with SFE ensured a great selectivity by avoiding the use of toxic organic solvents and thus consisted of a promising technique for sustainable production of Calendula extracts.

scholarly journals In Vitro Analysis of the Erosive Potential of Whey Protein

2020 ◽  
Vol 9 (9) ◽  
pp. e487997742
Author(s):  
Myrelle Leal Campos Sousa ◽  
Isabela Alice Soares de Medeiros ◽  
Liege Helena Freitas Fernandes ◽  
Smyrna Luiza Ximenes de Souza ◽  
Alidianne Fábia Cabral Cavalcanti ◽  
...  
Keyword(s):  
Whey Protein ◽  
Dental Enamel ◽  
Soluble Solids ◽  
Total Soluble Solids ◽  
Significant Difference ◽  
Vitro Analysis ◽  
In Vitro Analysis

The objective of this study was to evaluate the pH and total soluble solids (TSS) content of whey protein beverages. Nine products of three different flavors (vanilla, strawberry and chocolate) of the following brands Muke®, Best Whey®, and Essencial Nutrition Whey®, had their pH evaluated by potentiometry, and their TSS evaluated by refractometry. The lowest pH recorded was for Muke® Chocolate (5.75), and the highest was for Essencial Nutrition Whey® Cacao (6.91). Regarding TSS, the lowest average was for Essencial Nutrition Whey® Vanilla (10.17), and the highest was for Essencial Nutrition Whey® Red Berry (16.66). There was no statistically significant difference in pH and TSS among the flavors and brands evaluated. It was concluded that the analyzed beverages had a higher pH than the pH that is critical to dental enamel and the TSS content was considered high.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

1163: Radial Dilation of Ureteral Balloons: A Comparative in-Vitro Analysis

2005 ◽  
Vol 173 (4S) ◽  
pp. 315-316
Author(s):  
Kari Hendlin ◽  
Brynn Lund ◽  
Manoj Monga
Keyword(s):  
Vitro Analysis ◽  
In Vitro Analysis
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