scholarly journals Laser Controlled Cavitation Deformation in Red Blood Cells

2019 ◽  
Vol 2 (1) ◽  
pp. 29-31
Author(s):  
Tahira Ahmed ◽  
Yasir Jamil
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cavitation Bubble ◽  
High Speed ◽  
Research Work ◽  
Total Analysis System ◽  
System Method ◽  
Total Analysis ◽  
Analysis System ◽  
Intensity Laser

In this research work, we studied µTAS (µ total analysis system) method to measure the deformability of red blood cells .We took  the sample of RBCs(red blood cells) in a micro fluidic based chamber, then  allowed  low intensity laser of infra red region, mostly at wavelength 1064 nm, to fall on the sample. This generated a cavitation bubble in the fluid because of evaporation mechanism involved. We tightly hold the slides containing blood sample so that the displacement of RBCs (red blood cells) stay confined in the viewing area of the microscope. Firstly the deformation due to expansion of cavitation bubble was sighted and then came the restoring of shape of RBCs (red blood cells) which was possible due to a focused and pulsed laser. The RBCs restored their shape after some time and was viewed with the help of a video recorded by a high- speed camera. In this way the plastic character and elastic properties of RBCs (red blood cells) was measured.

scholarly journals Plasma skimming efficiency of human blood in the spiral groove bearing of a centrifugal blood pump

2020 ◽  
Author(s):  
Daisuke Sakota ◽  
Kazuki Kondo ◽  
Ryo Kosaka ◽  
Masahiro Nishida ◽  
Osamu Maruyama
Keyword(s):  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
High Speed ◽  
Blood Pump ◽  
Spiral Groove ◽  
Centrifugal Blood Pump ◽  
Plasma Skimming ◽  
Ridge Area ◽  
Spiral Groove Bearing

Abstract This work investigates the plasma skimming effect in a spiral groove bearing within a hydrodynamically levitated centrifugal blood pump when working with human blood having a hematocrit value from 0 to 40%. The present study assessed the evaluation based on a method that clarified the limitations associated with such assessments. Human blood was circulated in a closed-loop circuit via a pump operating at 4000 rpm at a flow rate of 5 L/min. Red blood cells flowing through a ridge area of the bearing were directly observed using a high-speed microscope. The hematocrit value in the ridge area was calculated using the mean corpuscular volume, the bearing gap, the cross-sectional area of a red blood cell, and the occupancy of red blood cells. The latter value was obtained from photographic images by dividing the number of pixels showing red blood cells in the evaluation area by the total number of pixels in this area. The plasma skimming efficiency was calculated as the extent to which the hematocrit of the working blood was reduced in the ridge area. For the hematocrit in the circuit from 0 to 40%, the plasma skimming efficiency was approximately 90%, meaning that the hematocrit in the ridge area became 10% as compared to that in the circuit. For a hematocrit of 20% and over, red blood cells almost completely occupied the ridge. Thus, a valid assessment of plasma skimming was only possible when the hematocrit was less than 20%.

Detection of Directivity of Fluorescence From a Mixed Specimen Which Consists of Micro Particles Attached Different Fluorescent Substances Using a Light Waveguide Incorporated Optical TAS

2018 ◽  
Author(s):  
Toshifumi Ohkubo ◽  
Nobuyuki Terada ◽  
Yoshikazu Yoshida
Keyword(s):  
Microfluidic Channel ◽  
Laser Source ◽  
Microfluidic Channels ◽  
Fluorescent Substance ◽  
Total Analysis System ◽  
Micro Particles ◽  
Total Analysis ◽  
Light Waveguide ◽  
Analysis System ◽  
Near Future

A resin-based optical total analysis system (O-TAS) which consists both of microfluidic channels and light waveguides [1] is thought to be one of the most promising components in developing a “ubiquitous human healthcare system” in the near future. Along with this technology trend, we have already developed a transparent epoxy-resin-based optical TAS chip which has a specially prepared light waveguide structure of radially arranged configuration at an intersection portion with a microfluidic channel, in order to detect directivity of fluorescence from fluorescent substance attached micro particles [2],[3]. Schematic diagram of the optical TAS is shown in Figure 1. In the latest research, utilizing an AC modulated laser source and time-series averaging function on detected signal waveforms, we could have successfully obtained directivities of fluorescence from 5-μm-diameter particles with higher signal to noise (S/N) ratio [3].

Micro Particle Imaging Velocimetry Measurements in High Aspect Ratio Passive Microfluidic Components

2005 ◽  
Author(s):  
Paul Chiarot ◽  
Pierre Sullivan ◽  
Ridha Ben Mrad
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Particle Imaging Velocimetry ◽  
Passive Component ◽  
Total Analysis System ◽  
Micro Piv ◽  
Micro Total Analysis Systems ◽  
Total Analysis ◽  
Analysis System ◽  
Particle Imaging

In this work, micro particle imaging velocimetry (micro-PIV) was performed on the fundamental components of a micro total analysis system. Specifically, high aspect ratio passive valves and mixers were designed, fabricated, and characterized. The components were built using Micralyne Protolyne technology on a glass substrate and operated at reasonably achievable pressures. The flows through the components were analyzed both qualitatively and quantitatively with the goal of developing a more complete understanding of internal device performance. Using the results of the micro-PIV developed velocity fields it was found that the high aspect ratio passive valves are able to perform at reasonably achievable pressures. However, it was determined that the high aspect ratio passive mixers offer limited performance enhancements because of the low Reynolds number flows. The results of this work contribute to the understanding of passive component operation and address some of the challenges associated with developing completely integrated micro total analysis systems that use passive devices.

scholarly journals Miniaturization of fluorescence sensing in optofluidic devices

2020 ◽  
Vol 24 (9) ◽  
Author(s):  
Daniel Măriuţa ◽  
Stéphane Colin ◽  
Christine Barrot-Lattes ◽  
Stéphane Le Calvé ◽  
Jan G. Korvink ◽  
...  
Keyword(s):  
Lab On A Chip ◽  
Time Data ◽  
Fluorescence Sensing ◽  
Successful Development ◽  
Total Analysis System ◽  
Total Analysis ◽  
Integration Strategies ◽  
Analysis System ◽  
On Chip ◽  
Industrial Sensing

Abstract Successful development of a micro-total-analysis system (µTAS, lab-on-a-chip) is strictly related to the degree of miniaturization, integration, autonomy, sensitivity, selectivity, and repeatability of its detector. Fluorescence sensing is an optical detection method used for a large variety of biological and chemical assays, and its full integration within lab-on-a-chip devices remains a challenge. Important achievements were reported during the last few years, including improvements of previously reported methodologies, as well as new integration strategies. However, a universal paradigm remains elusive. This review considers achievements in the field of fluorescence sensing miniaturization, starting from off-chip approaches, representing miniaturized versions of their lab counter-parts, continuing gradually with strategies that aim to fully integrate fluorescence detection on-chip, and reporting the results around integration strategies based on optical-fiber-based designs, optical layer integrated designs, CMOS-based fluorescence sensing, and organic electronics. Further successful development in this field would enable the implementation of sensing networks in specific environments that, when coupled to Internet-of-Things (IoT) and artificial intelligence (AI), could provide real-time data collection and, therefore, revolutionize fields like health, environmental, and industrial sensing.

Water glass bonding for micro-total analysis system

2002 ◽  
Vol 81 (2-3) ◽  
pp. 187-195 ◽  
Author(s):  
Takeshi Ito ◽  
Kazuharu Sobue ◽  
Seishiro Ohya
Keyword(s):  
Water Glass ◽  
Micro Total Analysis System ◽  
Total Analysis System ◽  
Total Analysis ◽  
Analysis System

A SU-8 microfluidic total analysis system integrating silicon photodiodes and buried waveguides

2005 ◽  
Author(s):  
P. de la Fuente ◽  
J.A. Etxeberria ◽  
J. Berganzo ◽  
M.T. Arroyo ◽  
E. Castano ◽  
...  
Keyword(s):  
Total Analysis System ◽  
Total Analysis ◽  
Analysis System
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