scholarly journals Modern Approaches to the Methods of Creating of Phage Basis for Treatment-Prophylactic a Drug of Bacteriophages Pseudomonas aeruginosa

2018 ◽  
Vol 17 (2) ◽  
pp. 37-45
Author(s):  
O. A. Polygach ◽  
N. N. Voroshilov ◽  
N. V. Tikunova ◽  
V. V. Morozova ◽  
A. Y. Tikunov ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Drug Design ◽  
Antimicrobial Therapy ◽  
Wide Spectrum ◽  
Morphological Properties ◽  
Genetic Groups ◽  
Pseudomonas Aeruginosa Infection ◽  
Cis Countries ◽  
Regions Of Russia

Therapeutic and prophylactic preparations of bacteriophages are considered as an effective modern means of antimicrobial therapy of Pseudomonas aeruginosa infection. Bacteriophages that are part  of the drugs must be certified for confirmation of their lytic and  genetic properties, which ensures the safety and effectiveness of the  use of drugs. In our study, biological and morphological  properties were studied for drug design, the spectrum of  antibacterial activity of bacteriophages isolated in various regions of  Russia was determined. The most important stage of the work was  research on ensuring genetic safety of bacteriophage preparations.  As a result, a composition of 6 strains of lytic bacteriophages of P.  aeruginosa (PaUfa №№ 1, 2, 3, 4, 6, 7) composed of the  morphology of the virions to the families Podoviridae and Myoviridae, the order of Caudovirales, phylogenetic genetic groups phiKMV- likevirus , N4-likevirus, PB1-likevirus. The composition has a wide  spectrum of antibacterial activity (90.7%) with respect to 818  collisional, epidemiologically significant hospital and clinical strains of P. aeruginosa bacteria, and also quite versatile for various remote  territories of Russia and CIS countries.

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

Identification of Optimal Combinations for Empirical Dual Antimicrobial Therapy of Pseudomonas aeruginosa Infection: Potential Role of a Combination Antibiogram

2006 ◽  
Vol 27 (4) ◽  
pp. 413-415 ◽  
Author(s):  
Mari Mizuta ◽  
Darren R. Linkin ◽  
Irving Nachamkin ◽  
Neil O. Fishman ◽  
Mark G. Weiner ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Therapy ◽  
Antimicrobial Therapy ◽  
Potential Role ◽  
Drug Combinations ◽  
Fine Tuning ◽  
Pseudomonas Aeruginosa Infection

To better determine the optimal combinations for empirical dual antimicrobial therapy of Pseudomonas aeruginosa infection, we evaluated the utility of a novel combination antibiogram. Although the combination antibiogram allowed modest fine-tuning of choices for dual antibiotic therapy, selections based on the 2 antibiograms did not differ substantively. Drug combinations with the broadest coverage were consistently composed of an aminoglycoside and a β-lactam.

scholarly journals Outpatient therapy with piperacillin/tazobactam using elastomeric pumps in patients with Pseudomonas aeruginosa infection

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jose Luis Lamas Ferreiro ◽  
Judith Álvarez Otero ◽  
Ana Sanjurjo Rivo ◽  
Lucía González González ◽  
Irene Rodríguez Conde ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Continuous Infusion ◽  
Antimicrobial Therapy ◽  
Clinical Cure Rate ◽  
Hospital Treatment ◽  
Cure Rate ◽  
End Of Treatment ◽  
Pseudomonas Aeruginosa Infection ◽  
The Cost ◽  
Theoretical Cost

AbstractThe aim of this study was to evaluate the efficacy and safety of outpatient antimicrobial therapy with piperacillin-tazobactam in continuous infusion using elastomeric pumps and to evaluate the economic impact compared with conventional hospital treatment in patients with Pseudomonas aeruginosa (PA) infections. This is an observational study. Patients with PA infection treated with continuous piperacillin-tazobactam infusion using elastomeric pumps in our hospital between January 2015 and December 2017 were included. Primary outcomes were mortality during antibiotic treatment and mortality at 30 days. Secondary outcomes were reinfection or relapse at 30 days and clinical cure rate. The cost of each episode was compared with theoretical cost of the same treatment using conventional hospitalization. 35 patients were included. One patient (2.9%) died during the treatment. Overall 30-day mortality was 5.7%. No death was related to infection by PA. One patient (2.9%) had a reinfection at 30 days. Cure was achieved in 93% of patients at the end of treatment. There were no severe complications related to elastomeric pumps. Treatment cost with outpatient antimicrobial therapy was 67% lower than theoretical cost with conventional hospital treatment. Oupatient antimicrobial therapy with piperacillin-tazobactam in continuous infusion using elastomeric pumps in patients with PA infections is safe and effective with lower costs.

The effect of the antiinflammatory IL-1R antagonist anakinra in mice with CF-like lung disease and Pseudomonas aeruginosa infection

Pneumologie ◽  
2016 ◽  
Vol 70 (07) ◽  
Author(s):  
A Schütte ◽  
Z Zhou-Suckow ◽  
J Schatterny ◽  
S Schmidt ◽  
S Hassel ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lung Disease ◽  
Pseudomonas Aeruginosa Infection

Antibacterial Activity of Desert Truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa

2017 ◽  
Vol 19 (2) ◽  
pp. 121-125 ◽  
Author(s):  
Domenico Schillaci ◽  
Maria Grazia Cusimano ◽  
Stella Maria Cascioferro ◽  
Vita Di Stefano ◽  
Vincenzo Arizza ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Desert Truffles

Green synthesis, characterization of silver sulfide nanoparticles and antibacterial activity evaluation

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Antibacterial Activity ◽  
Large Scale ◽  
Research Work ◽  
Silver Sulfide ◽  
Morphological Properties ◽  
Effective Diameter ◽  
Novel Approach ◽  
Transmission Electron ◽  
Green Route ◽  
Silver Sulfide Nanoparticles

This research work presents a facile and green route for synthesis silver sulfide (Ag2SNPs) nanoparticles from silver nitrate (AgNO3) and sodium sulfide nonahydrate (Na2S.9H2O) in the presence of rosemary leaves aqueous extract at ambient temperature (27 oC). Structural and morphological properties of Ag2SNPs nanoparticles were analyzed by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The surface Plasmon resonance for Ag2SNPs was obtained around 355 nm. Ag2SNPs was spherical in shape with an effective diameter size of 14 nm. Our novel approach represents a promising and effective method to large scale synthesis of eco-friendly antibacterial activity silver sulfide nanoparticles.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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