scholarly journals Molecular-genetic analysis of diff erent species of lace bugs (Hеteroptera: Tingidae) by RAPD-markers

2019 ◽  
pp. 21-25
Author(s):  
E. N. Besedinа ◽  
V. I. Kil
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationship ◽  
Dna Polymorphism ◽  
Rapd Markers ◽  
Molecular Genetic ◽  
Genetic Diagnosis ◽  
Research Process ◽  
Molecular Genetic Analysis ◽  
Pcr Analysis ◽  
Lace Bugs

Studying the genetics of harmful insects populations is of great importance in understanding the migratory processes of species, especially invasive ones, and the fl ow of genes between populations. In this regard, one of the priorities of the genetics of arthropod populations is to assess the genetic similarity of individuals, genetic diversity, and DNApolymorphism. Universal RAPD-primers (OPA07, OPA09 and OPA18) for lace bugs (Tingidae family) have been revealed. In order to study DNA-polymorphism and the genetic diversity of lace bugs using selected primers, a comparative PCR-analysis of four species of bugs of this family (Corythucha arcuata Say, Corythucha ciliata Say, Stephanitis pyri F., Monosteira unicostata Mulsant et Rey) was carried out. It was shown that the species Monosteara unicostata diff ered from the others in the lowest level of DNA polymorphism and genetic diversity. The high values of DNA polymorphism and genetic diversity of the other three species indicate a high migratory ability of these insect species and a signifi cant intraspecifi c gene drift. In the research process, clustering of lace bugs species based on the data obtained using RAPD-markers was carried out in order to determine their genetic relationship. Cluster analysis of the data was performed by the UPGMA-method using the Popgene program. We found out that the genetically closest species were the species of the genus Corythucha, and the most distant from others was the species Monosteira unicostata. It was shown that RAPD-PCR-method can be successfully used in the analysis of interspecifi c diff erences of insects, along with other molecular-genetic methods. The performed studies allowed us to assess the eff ectiveness of the RAPD-primers identifi ed in the work for diff erentiating the species of lace bugs and to obtain information on the genetic relationship of the Tingidae family species. The primers identifi ed in the work are also eff ective for assessing DNA-polymorphism and genetic diversity of insects of the Tingidae family. This method of molecular-genetic diagnosis allows carrying out a more eff ective monitoring of pests of this insect family.

Study on genetic diversity of Castanopsis phuthoensis Luong in Phu Tho province, Vietnam by RAPD markers

2016 ◽  
Vol 14 (2) ◽  
pp. 237-243
Author(s):  
Nguyễn Văn Thọ ◽  
Lê Thị Mai Linh ◽  
Nguyễn Viễn ◽  
Phạm Quang Tiến
Keyword(s):  
Genetic Diversity ◽  
Tree Species ◽  
Dna Polymorphism ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Analysis ◽  
Dna Fragments ◽  
Similarity Coefficients ◽  
Rapd Pcr ◽  
Rapd Primer

Castanopsis phuthoensis Luong is an endemic tree species of Phu Tho province, only distributed narrowly in forest rehabilitation in two communes belonging to Doan Hung district with density of this species is very low, only from 3.1 to 11.1 trees per hectare. Diameter distributions of the species of number of trees is characterized by curve style with a peak in 20cm or 24cm diameter classes. As it is difficult to find the seedling in the nature, research on forest structure, relations between tree species and genetic diversity is very necessary to define method of conservation for this species. In this study, we used RAPD markers to study on genetic diversity of Castanopsis phuthoensis Luong. The random amplified polymorphic DNA (RAPD) based on the polymeraze chain reaction (PCR) detects nucleotide sequence polymorphism using a single 10mer of arbitrary nucleotided sequence. The RAPD technology has quickly gained widespread acceptance and application because it providesa tool for genetic analysis that have not previously benefited the use of molecular markers. In this study, ten random primers to analyse the genetic diversity of 15 Castanopsis phuthoensis Luong samples that were collected from Phu Tho province, Vietnam. 9 RAPD primers gave DNA polymorphism and 01 RAPD primer OPA20 gave not DNA polymorphism. In the analysis region 0.25-2 kb, there were 56 DNA fragments amplified and 34 DNA fragments were polymorphic. Genetic similarity coefficients of 15 Castanopsis phuthoensis Luong samples ranged from 0.55- 0.95. The phylogenetic tree of 15 samples are divided into two main groups. As results of RAPD-PCR analysis, these samples were collected from the homologous geographical locations and the genetic diversity of 15 samples is not high. Therefore, it is necessary to conserve the "Castanopsis phuthoensis Luong".

scholarly journals The pattern of genetic diversity of different breeds of pigs based on microsatellite analysis

2020 ◽  
Vol 24 (7) ◽  
pp. 747-754
Author(s):  
V. R. Kharzinova ◽  
N. A. Zinovieva
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Molecular Genetic ◽  
Genetic Material ◽  
Animal Husbandry ◽  
Molecular Genetic Analysis ◽  
Fixation Index ◽  
Large White ◽  
Black And White ◽  
The Republic

One of the main tasks of genetics and animal breeding is the assessment of genetic diversity and the study of genetic relationships between different breeds and populations using molecular genetic analysis methods. We analysed the polymorphism of microsatellites and the information on the state of genetic diversity and the population structure of local breeds in Russia: the Kemerovo, the Berkshire, the Liven, the Mangalitsa, and the Civilian; in the Republic of Belarus: the Large White and the Black-and-White; and in Ukraine: the White Steppe, as well as commercial breeds of imported origin of domestic reproduction: the Large White, the Landrace, and the Duroc. The materials used for this study were the tissue and DNA samples extracted from 1,194 pigs and DNA of the UNU “Genetic material bank of domestic and wild animal species and birds” of the L.K. Ernst Federal Research Center for Animal Husbandry. Polymorphisms of 10 microsatellites (S0155, S0355, S0386, SW24, SO005, SW72, SW951, S0101, SW240, and SW857) were determined according to the previously developed technique using DNA analyser ABI3130xl. To estimate the allele pool of each population, the average number of alleles (NA), the effective number of alleles (NE ) based on the locus, the rarified allelic richness (AR), the observed (HO ) and expected (HE ) heterozygosity, and the fixation index (FIS) were calculated. The degree of genetic differentiation of the breeds was assessed based on the pairwise values of FST and D. The analysis of the allelic and genetic diversity parameters of the local breeds showed that the maximum and minimum levels of polymorphism were observed in pigs of the Ukrainian White Steppe breed (NA = 6.500, NE = 3.709, and AR = 6.020) and in pigs of the Duroc breed (NA = 4.875, NE = 2.119, and AR = 3.821), respectively. The highest level of genetic diversity was found in the Large White breed of the Republic of Belarus (HO = 0.707 and NE = 0.702). The minimum level of genetic diversity was found in pigs of the imported breeds – the Landrace (HO = 0.459, HE = 0.400) and the Duroc (HO = 0.480, HE = 0.469) – indicating a high selection pressure in these breeds. Based on the results of phylogenetic analysis, the genetic origin of Large White pigs, the breeds, from which the Berkshire pigs originated, and the genetic detachment of the Landrace from the Mangalitsa breeds were revealed. The cluster analysis showed a genetic consolidation of the Black-and-White, the Berkshire, and the Mangalitsa pigs. Additionally, the imported breeds with clustering depending on the origin were characterised by a genetic structure different from that of the other breeds. The information obtained from these studies can serve as a guide for the management and breeding strategies of the pig breeds studied, to allow their better use and conservation.

Molecular Genetic Analysis of a Compound Heterozygote for the Glycoprotein (GP) IIb Gene Associated With Glanzmann’s Thrombasthenia: Disruption of the 674-687 Disulfide Bridge in GPIIb Prevents Surface Exposure of GPIIb-IIIa Complexes

Blood ◽  
1999 ◽  
Vol 93 (3) ◽  
pp. 866-875 ◽  
Author(s):  
Consuelo González-Manchón ◽  
Marta Fernández-Pinel ◽  
Elena G. Arias-Salgado ◽  
Milagros Ferrer ◽  
M.-Victoria Alvarez ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Splice Junction ◽  
Disulfide Bridge ◽  
Molecular Genetic Analysis ◽  
Pcr Analysis ◽  
Polymorphism Analysis ◽  
Glanzmann’S Thrombasthenia ◽  
Single Stranded Conformational Polymorphism ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Content

Abstract This work was aimed at elucidating the molecular genetic lesion(s) responsible for the thrombasthenic phenotype of a patient whose low platelet content of glycoprotein (GP) IIb-IIIa indicated that it was a case of type II Glanzmann’s thrombasthenia (GT). The parents did not admit consanguinity and showed a reduced platelet content of GPIIb-IIIa. Polymerase chain reaction (PCR)–single-stranded conformational polymorphism analysis of genomic DNA showed no mutations in the patient’s GPIIIa and two novel mutations in the GPIIb gene: one of them was a heterozygous splice junction mutation, a C→A transversion, at position +2 of the exon 5-intron 5 boundary [IVS5(+2)C→A] inherited from the father. The predicted effect of this mutation, insertion of intron 5 (76 bp) into the GPIIb-mRNA, was confirmed by reverse transcription-PCR analysis of platelet mRNA. The almost complete absence of this mutated form of GPIIb-mRNA suggests that it is very unstable. Virtually all of the proband’s GPIIb-mRNA was accounted for by the allele inherited from the mother showing a T2113→C transition that changes Cys674→Arg674 disrupting the 674-687 intramolecular disulfide bridge. The proband showed a platelet accumulation of proGPIIb and minute amounts of GPIIb and GPIIIa. Moreover, transfection and immunoprecipitation analysis demonstrated that [Arg674]GPIIb is capable of forming a heterodimer complex with GPIIIa, but the rate of subunit maturation and the surface exposure of GPIIb-IIIa are strongly reduced. Thus, the intramolecular 674-687 disulfide bridge in GPIIb is essential for the normal processing of GPIIb-IIIa complexes. The additive effect of these two GPIIb mutations provides the molecular basis for the thrombasthenic phenotype of the proband.

Molecular Genetic Analysis of a Compound Heterozygote for the Glycoprotein (GP) IIb Gene Associated With Glanzmann’s Thrombasthenia: Disruption of the 674-687 Disulfide Bridge in GPIIb Prevents Surface Exposure of GPIIb-IIIa Complexes

Blood ◽  
1999 ◽  
Vol 93 (3) ◽  
pp. 866-875 ◽  
Author(s):  
Consuelo González-Manchón ◽  
Marta Fernández-Pinel ◽  
Elena G. Arias-Salgado ◽  
Milagros Ferrer ◽  
M.-Victoria Alvarez ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Splice Junction ◽  
Disulfide Bridge ◽  
Molecular Genetic Analysis ◽  
Pcr Analysis ◽  
Polymorphism Analysis ◽  
Glanzmann’S Thrombasthenia ◽  
Single Stranded Conformational Polymorphism ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Content

This work was aimed at elucidating the molecular genetic lesion(s) responsible for the thrombasthenic phenotype of a patient whose low platelet content of glycoprotein (GP) IIb-IIIa indicated that it was a case of type II Glanzmann’s thrombasthenia (GT). The parents did not admit consanguinity and showed a reduced platelet content of GPIIb-IIIa. Polymerase chain reaction (PCR)–single-stranded conformational polymorphism analysis of genomic DNA showed no mutations in the patient’s GPIIIa and two novel mutations in the GPIIb gene: one of them was a heterozygous splice junction mutation, a C→A transversion, at position +2 of the exon 5-intron 5 boundary [IVS5(+2)C→A] inherited from the father. The predicted effect of this mutation, insertion of intron 5 (76 bp) into the GPIIb-mRNA, was confirmed by reverse transcription-PCR analysis of platelet mRNA. The almost complete absence of this mutated form of GPIIb-mRNA suggests that it is very unstable. Virtually all of the proband’s GPIIb-mRNA was accounted for by the allele inherited from the mother showing a T2113→C transition that changes Cys674→Arg674 disrupting the 674-687 intramolecular disulfide bridge. The proband showed a platelet accumulation of proGPIIb and minute amounts of GPIIb and GPIIIa. Moreover, transfection and immunoprecipitation analysis demonstrated that [Arg674]GPIIb is capable of forming a heterodimer complex with GPIIIa, but the rate of subunit maturation and the surface exposure of GPIIb-IIIa are strongly reduced. Thus, the intramolecular 674-687 disulfide bridge in GPIIb is essential for the normal processing of GPIIb-IIIa complexes. The additive effect of these two GPIIb mutations provides the molecular basis for the thrombasthenic phenotype of the proband.

scholarly journals MONITORING OF Aegilops L. LOCAL SPECIES GENETIC DIVERSITY OF KAZAKHSTAN’S FLORA

2018 ◽  
Vol 22 (4) ◽  
pp. 484-490 ◽  
Author(s):  
R. Urazaliev ◽  
M. Yessimbekova ◽  
K. Mukin ◽  
A. Chirkin ◽  
G. Ismagulova
Keyword(s):  
Genetic Diversity ◽  
Global Climate ◽  
Molecular Genetic ◽  
Crop Wild Relatives ◽  
Molecular Genetic Analysis ◽  
Ex Situ ◽  
Marker Allele ◽  
New Genes ◽  
Local Species ◽  
Allelic Variations

Cereal Crop Wild Relatives (CWR) are a very  important gene pool for cereal/wheat improvement. New genes for resistance to diseases and pests are urgently needed to avoid using pesticides and to raise adaptivity to the environmental stresses caused by global climate change. In this regard, the study is aimed at ex situ conservation of Aegilops L. genus local ecotypes’ genetic diversity, which is very relevant and promising for breeding. In order to establish breeding utility and form an ex situ collection reflecting the intra- and inter-specific diversity, the phenotypic screening of Kazakhstan’s local populations of Aegilops L. genus (Ae. cylindrica, Ae. tauschii, Ae. triuncialis and Ae. crassa) was conducted on the basis of multiple indicators. For the first time molecular-genetic analysis of 50 representatives of Aegilops L. genus from Kazakhstan’s flora was performed. The microsatellite analysis with the use of 11 EST-SSR markers revealed eight of them to be most effective. For each marker, allele frequency and average heterozygosity was calculated. For the most informative markers the presence of 5 and 6 respective allelic variations was found. A bank of genomic DNA was created and kept in ex situ storage (–70 °С, long-term) in the IMBB of the MES of RK.

scholarly journals Evaluation of genetic diversity of honey bee in Ukraine analyzed by the SSR-markers

2020 ◽  
Vol 26 ◽  
pp. 56-60
Author(s):  
D. I. Hryhorchuk ◽  
A. M. Rabokon ◽  
A. S. PostovoitovA ◽  
N. M. Pirko ◽  
Ya. V. Pirko ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Honey Bee ◽  
Ssr Markers ◽  
Honey Bees ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Analysis Method ◽  
Ssr Analysis ◽  
Silver Nitrate Staining

Aim. The aim of the work was to analyze current genetic structure of honey bee populations in Ukraine that belong to different subspecies: A. meliffera meliffera, A. meliffera carnica, A. meliffera macedonica using microsatellite markers. Methods. SSR-analysis was used for evaluation of the honey bee polymorphism. Amplified fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The analysis of the sample of honey bees (workers and male-bees) collected from different regions of Ukraine was performed by using two SSR-markers (Ac011 and A007). In this sample reasonably high polymorphism was observed, especially for the SSR-marker A007. Conclusions. It was estimated that SSR-analysis method can be applied in molecular-genetic analysis of honey bees for evaluation of genetic diversity and cross-subspecies hybridization. Keywords: microsatellite markers, Apis meliffera, PIC (Polymorphism Information Content).

scholarly journals Molecular genetic analysis of some North African barley germplasms

2017 ◽  
Vol 109 (2) ◽  
pp. 187
Author(s):  
Reda Gaafar ◽  
Mai Allam ◽  
Rasha Sabry ◽  
Mahmoud Saker
Keyword(s):  
Rapd Markers ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
North African ◽  
Rt Pcr ◽  
Breeding Selection ◽  
High Level ◽  
Barley Genotypes ◽  
Selection Of ◽  
Selection Of Resistance

<p>Isozyme and RAPD markers were used to characterize 29 barley accessions, which were collected from North Africa. In addition, resistance gene sequences were employed to develop molecular markers using RT-PCR approach. High level of polymorphism was found with both RAPD and isozyme markers, where RAPD showed that 60 % of amplified bands were polymorphic. Peroxidase showed three polymorphic loci (7 allelic bands). Isozymes cluster analysis successfully separated the barley accessions into three geographically distinct groups. RAPD investigation demonstrated that Egyptian accessions were grouped into two obvious groups. Moreover, the Tunisian accessions showed no distinct clustering, while high dissimilarities were revealed by the Algerian accessions. In the RT-PCR, from six primer pairs selected, primer pair AF092524P1P2 successfully amplified two specific amplicons of approximately (340 &amp; 220 bp) and (360 &amp; 270 bp), respectively in two Egyptian barley genotypes (El-Awamah and Awlad-Ali). One primer pair DN988165P1P2 gave only one specific amplicon in both barley genotypes of 250 and 270 bp, respectively. The markers developed could be used in improving barley crop by assisting in breeding selection of resistance genotypes.</p>

scholarly journals Molecular Analysis of Hexaploid Wheat (Triticum aestivum L.) Cultivars by RAPD Markers

1970 ◽  
Vol 19 (1) ◽  
pp. 35-44 ◽  
Author(s):  
S. Mitra ◽  
K. M. Nasiruddin ◽  
E. H. Chowdhury
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Molecular Genetic ◽  
Triticum Aestivum L ◽  
Molecular Genetic Analysis ◽  
Wheat Cultivars ◽  
Polymorphic Loci ◽  
Upgma Dendrogram ◽  
Nei's Genetic Distance

RAPD assay was conducted for molecular genetic analysis of six wheat cultivars, such as, Kanchan, Sourav, Gourab, Shatabdi, Pavon and BAW-1006 to observe genetic variability and relatedness among these cultivars. Three out of 12 decamer random primers showed distinctly polymorphic bands when used to amplify genomic DNA. The primers yielded a total of 23 RAPD markers of which 14 were considered as polymorphic. The proportion of polymorphic loci and gene diversity (h) values were 34.78% and 0.153 for BAW-1006, 30.43% and 0.124 for Kanchan, 26.09% and 0.127 for Shatabdi, 26.09% and 0.127 for Pavon, 26.09% and 0.111 for Gourab, 21.74% and 0.098 for Sourav, respectively. The coefficient of gene differentiation (Gst) and gene flow (Nm) values across all the loci were 0.50 and 0.50, respectively indicating genetic divergence among populations. The UPGMA  dendrogram  based  on Nei’s  genetic distance, grouped  six cultivars into two main clusters:  Kanchan, Sourav, Gourab  and Shatabdi  in cluster I; Pavon and BAW-1006 in cluster II. The cluster I was further separated: Kanchan alone in sub-cluster I and Sourav, Gourab, Shatabdi in sub-cluster II; furthermore, Sourab and Gourab grouped together in sub-sub-cluster I of sub-cluster II with the lowest genetic distance of 0.035. Thus, RAPD offer a potentially simple, rapid and reliable method to evaluate genetic variation and relatedness among six wheat cultivars.  Key words: RAPD, genetic diversity, polymorphic loci, wheat D.O.I. 10.3329/ptcb.v19i1.4915 Plant Tissue Cult. & Biotech. 19(1): 35-44, 2009 (June)

scholarly journals Genetic Relationship Among Ten Promising Eggplant Varieties Using RAPD Markers

1970 ◽  
Vol 19 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Md. Sanaullah Biswas ◽  
Md. Abdullah Yousuf Akhond ◽  
Md. Al-Amin ◽  
Mahmuda Khatun ◽  
Muhammed Rezwan Kabir
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Relationship ◽  
Plant Tissue ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Similarity Index ◽  
The Other ◽  
Upgma Dendrogram ◽  
Potential Tool

RAPD technique was used as a tool for assessing genetic diversity and varietal relationships among ten varieties of eggplant. Out of 21 primers screened four were selected. With these primers 76 clear and bright fragments were obtained of which 44 fragments considered polymorphic. The proportion of polymorphic loci and gene diversity values across all loci were 57.89% and 0.23, respectively. The UPGMA dendrogram based on genetic distance segregated the ten varieties of eggplant into two main clusters. Dohazari, Kazla, Nayantara and ISD-006 were grouped together in cluster I whereas Uttara, Islampuri, Khatkhatia, Singnath, BARI Begun-08 and Eggplant Line-083 into cluster II. Kazla and Nayantara variety pair was very close to each other with the highest intervarietal similarity index (92.54%) and lowest genetic distance (0.14). On the other hand, Khatkhatia and Nayantara pair was the lowest intervarietal similarity index (41.67%) with highest genetic distance (0.48). Therefore, identification of genetically distinct varieties using RAPD markers could be a potential tool for eggplant improvement. Key words: Eggplant, Polymorphism, Genetic relationship, RAPD D.O.I. 10.3329/ptcb.v19i2.5006 Plant Tissue Cult. & Biotech. 19(2): 119-126, 2009 (December)

scholarly journals Genetic and physiological analysis T1 biotechnological plants of winter wheat (Triticum aestivum L.)

2020 ◽  
Vol 26 ◽  
pp. 222-227
Author(s):  
A. G. Komisarenko ◽  
S. I. Mykhalska ◽  
V. M. Kurchii ◽  
O. O. Khrystan
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
First Generation ◽  
Genetically Modified ◽  
Molecular Genetic ◽  
Triticum Aestivum L ◽  
Molecular Genetic Analysis ◽  
Pcr Analysis ◽  
Proline Dehydrogenase ◽  
In Planta

Aim. To investigate inheritance of transgenes in the first generation (T1) of winter wheat biotechnological plants (Triticum aestivum L.). Analyze the performance of T1 genetically modified plants with a double stranded RNA suppressor of the proline dehydrogenase (pdh) gene under normal growing conditions. Methods. PCR analysis, DNA electrophoresis; determination of indicators of the structure of the crop. Results. Molecular genetic analysis was performed and the performance indicators of control and T1 biotechnological plants were investigated. Conclusions. The first generation of genetically modified winter wheat plants resulting from Agrobacterium-mediated transformation in planta confirmed the inheritance of integrated genes. Among the transgenic variants identified plants that lack some fragments of the target gene required for partial suppression of the gene of proline dehydrogenase wheat. It is shown that at the optimal terms of growing biotechnological plants of wheat winter-annual of UK 322/17 and UK 209 h was characterized by the best indexes of structure of harvest as compared to an initial form, while the genetically changed plants of genotypes of UK 95/17 and UK 065 after the elements of the productivity did not differ from control plants. Keywords: Triticum aestivum L., biotechnological plants, T-DNA, proline dehydrogenase gene, structural analysis indicators.

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