Estimating degradation of individual essential amino acids in fish meal and blood meal by rumen microbes in a dual-flow continuous-culture system

It was found that when a stable rumen fermentation, established in a continuous culture apparatus, was subjected to severe phosphorus deficiency, there were marked reductions in protozoal numbers, volatile fatty acid and ATP concentrations, and increased pH and ammonia concentrations, indicating reduced fermentative and synthetic capacities of the rumen microbes. Key words: Continuous culture, rumen fermentation, phosphorus deficiency

Download Full-text

Kinetics of biodegradation of Di-n-butyl phthalate in continuous culture system

Chemosphere ◽

10.1016/s0045-6535(98)00043-5 ◽

1998 ◽

Vol 37 (2) ◽

pp. 257-264 ◽

Cited By ~ 25

Author(s):

Jianlong Wang ◽

Ping Liu ◽

Hanchang Shi ◽

Yi Qian

Keyword(s):

Continuous Culture ◽

Culture System ◽

Continuous Culture System ◽

Butyl Phthalate ◽

Kinetics Of

Download Full-text

Evaluation of Soybean Meal, Corn Gluten Meal, Blood Meal and Fish Meal as Sources of Nitrogen and Amino Acids Disappearing from the Small Intestine of Steers

Journal of Animal Science ◽

10.2527/jas1989.671262x ◽

1989 ◽

Vol 67 (1) ◽

pp. 262 ◽

Cited By ~ 69

Author(s):

Evan C. Titgemeyer ◽

Neal R. Merchen ◽

Larry L. Berger

Keyword(s):

Amino Acids ◽

Small Intestine ◽

Soybean Meal ◽

Blood Meal ◽

Fish Meal ◽

Corn Gluten Meal ◽

Corn Gluten

Download Full-text

Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota

Journal of Microbiological Methods ◽

10.1016/j.mimet.2013.11.015 ◽

2014 ◽

Vol 96 ◽

pp. 111-118 ◽

Cited By ~ 21

Author(s):

David Feria-Gervasio ◽

William Tottey ◽

Nadia Gaci ◽

Monique Alric ◽

Jean-Michel Cardot ◽

...

Keyword(s):

Gut Microbiota ◽

Continuous Culture ◽

Culture System ◽

Human Gut ◽

Human Gut Microbiota ◽

Continuous Culture System

Download Full-text

Effects of lipopolysaccharide dosing on bacterial community composition and fermentation in a dual-flow continuous culture system

Journal of Dairy Science ◽

10.3168/jds.2018-14807 ◽

2019 ◽

Vol 102 (1) ◽

pp. 334-350 ◽

Cited By ~ 4

Author(s):

X. Dai ◽

E.M. Paula ◽

A.L.J. Lelis ◽

L.G. Silva ◽

V.L.N. Brandao ◽

...

Keyword(s):

Bacterial Community ◽

Continuous Culture ◽

Community Composition ◽

Culture System ◽

Bacterial Community Composition ◽

Continuous Culture System

Download Full-text

Design and development of a continuous culture system for studying rumen fermentation

Agricultural and Food Science ◽

10.23986/afsci.72366 ◽

1989 ◽

Vol 61 (5) ◽

pp. 463-473

Author(s):

Harri Miettinen ◽

Jouko Setälä

Keyword(s):

Continuous Culture ◽

Culture System ◽

Artificial Saliva ◽

Present Report ◽

Ammonia Concentration ◽

Rumen Fermentation ◽

Liquid Volume ◽

Desktop Computer ◽

Continuous Culture System

The present report describes an in vitro continuous culture system to simulate rumen fermentation. The complete assembly consists of six culture vessels (liquid volume 700 ml) fed twice daily with finely ground feed. The artificial saliva enters the vessel continuously, and the effluent leaves it continuously through the overflow port. The intermittent stirring of the fermentor content and the pH regulation are automatically controlled by a desktop computer. Two replicate experiments with ten fermentors given a diet of silage (50 %) and barley (50 %) were made in order to evaluate the system. The results indicate that the system reaches steady-state conditions within three to five days, ammonia concentration being an exeption. It takes for the ammonia concentration approximately 11—14 days to stabilize. The plateau values for the total volatile fatty acid (VFA) concentrations, the molar proportions of individual VFAs, and the ammonia concentrations were found to be within the accepted range in the rumen of animals given similar diets or in other artificial rumen systems. There was a tenfold decrease in the numbers of protozoa in the fermentors during the first four days of incubation. However, the average plateau value for the protozoa numbers (2.5 x 104/ml) is in the same range as in the dual flow continuous culture systems. The efficiency of the microbial N production was higher than that usually observed in vivo or in vitro (45 vs. 30 g/kg organic matter digested). The results indicate that this continuous culture system provides a reasonable estimate of rumen fermentation.

Download Full-text