scholarly journals Effect of pegbovigrastim on fertility and culling in grazing dairy cows and its association with prepartum nonesterified fatty acids

2021 ◽  
Author(s):  
Joaquín Barca ◽  
Ana Meikle ◽  
Mette Bouman ◽  
Ynte H. Schukken
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Nonesterified Fatty Acids ◽  
Grazing Dairy Cows

scholarly journals Sirtuin 3 Restores Synthesis and Secretion of Very Low-Density Lipoproteins in Cow Hepatocytes Challenged with Nonesterified Fatty Acids In Vitro

2021 ◽  
Vol 8 (7) ◽  
pp. 121
Author(s):  
Dongmei Xing ◽  
Baogen Wang ◽  
Hong Lu ◽  
Tao Peng ◽  
Jianming Su ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Low Density Lipoproteins ◽  
Mrna Abundance ◽  
Low Density ◽  
Sirtuin 3 ◽  
Very Low Density Lipoproteins ◽  
Nonesterified Fatty Acids ◽  
Tag Accumulation

Fatty liver is closely associated with elevated concentrations of nonesterified fatty acids (NEFA) and a low level of very low-density lipoproteins (VLDL) in blood of dairy cows. High NEFA inhibit the VLDL synthesis and assembly, and cause hepatic triacylglycerol (TAG) deposition. Sirtuin 3 (SIRT3), a mitochondrial deacetylase, antagonizes NEFA-induced TAG accumulation through modulating expressions of fatty acid synthesis and oxidation genes in cow hepatocytes. However, the role of SIRT3 in the VLDL synthesis and assembly was largely unknown. Here we aimed to test whether SIRT3 would recover the synthesis and assembly of VLDL in cow hepatocytes induced by high NEFA. Primary cow hepatocytes were isolated from 3 Holstein cows. Hepatocytes were infected with SIRT3 overexpression adenovirus (Ad-SIRT3), SIRT3-short interfering (si) RNA, or first infected with Ad-SIRT3 and then incubated with 1.0 mM NEFA (Ad-SIRT3 + NEFA). Expressions of key genes in VLDL synthesis and the VLDL contents in cell culture supernatants were measured. SIRT3 overexpression significantly increased the mRNA abundance of microsomal triglyceride transfer protein (MTP), apolipoprotein B100 (ApoB100) and ApoE (p < 0.01), and raised VLDL contents in the supernatants (p < 0.01). However, SIRT3 silencing displayed a reverse effect in comparison to SIRT3 overexpression. Compared with NEFA treatment alone, the Ad-SIRT3 + NEFA significantly upregulated the mRNA abundance of MTP, ApoB100 and ApoE (p < 0.01), and increased VLDL contents in the supernatants (p < 0.01). Our data demonstrated that SIRT3 restored the synthesis and assembly of VLDL in cow hepatocytes challenged with NEFA, providing an in vitro basis for further investigations testing its feasibility against hepatic TAG accumulation in dairy cows during the perinatal period.

Milk production and composition, and methane emissions from dairy cows fed lucerne hay with forage brassica or chicory

2016 ◽  
Vol 56 (3) ◽  
pp. 304 ◽  
Author(s):  
S. R. O. Williams ◽  
P. J. Moate ◽  
M. H. Deighton ◽  
M. C. Hannah ◽  
W. J. Wales ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Production ◽  
Perennial Grass ◽  
Milk Fatty Acids ◽  
Ch4 Emissions ◽  
Holstein Friesian ◽  
Grazing Dairy Cows ◽  
Diet Type ◽  
Friesian Cows

Forage brassica and chicory crops provide an alternative to perennial grass pastures as a forage supply for grazing dairy cows during summer, but there is little information about their effects on milk production and methane (CH4) emissions. Thirty-two Holstein–Friesian cows were fed for 10 days on a diet of lucerne cubes (750 g/kg DM) and grain (250 g/kg DM) (CON) or diets in which forage brassica (410 g/kg DM, FBR) or reproductive-stage chicory (410 g/kg DM, RCH) were offered with lucerne cubes (340 g/kg DM) and grain (250 g/kg DM). Cows offered the FBR diet produced more energy-corrected milk (25.4 kg/day) than did cows offered the CON diet (22.7 kg/day, P = 0.001), even though DM intake was not different for cows between the two groups (20.6 kg/day on average). In contrast, cows offered the RCH diet produced less energy-corrected milk (19.3 kg/day) than did cows in the other two groups (P = 0.001), reflecting the lower DM intake by cows offered the RCH diet (17.7 kg/day, P < 0.01). Methane yield (g CH4/kg DMI) was lower (P < 0.01) on the CON (21.0) and FBR (20.5) diets than on the RCH diet (26.1). Methane intensity (g/kg energy-corrected milk) was different (P < 0.01) for all diets, with CON (19.4) being intermediate, FBR (17.3) lowest and RCH (23.8) the greatest. Diet type was associated with differences in the proportions of only a small number of specific milk fatty acids, and differences in proportions of specific fatty acids were not related to CH4 emissions.

Effect of cereal grain and fibre supplements on the fatty acid composition of milk fat of grazing dairy cows in early lactation

2003 ◽  
Vol 70 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Chakra Wijesundera ◽  
Zhiping Shen ◽  
William J Wales ◽  
Dawn E Dalley
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Acid Composition ◽  
Milk Fat ◽  
Physiological State ◽  
Early Lactation ◽  
Cereal Grain ◽  
Grazing Dairy Cows

Two experiments were undertaken to determine the effects of cereal grain and fibre (hay or straw) supplements on the fatty acid composition of milk fat of grazing dairy cows in early lactation. In both experiments, grain supplements significantly increased (P<0·05) the proportion of the endogenously synthesized 10[ratio ]0–16[ratio ]0 fatty acids. Of the C18 acids, the proportion of 18[ratio ]0 and 18[ratio ]3 was significantly decreased (P<0·05) by grain supplementation, while that of 18[ratio ]2 was significantly increased (P<0·05). Irrespective of diet, 18[ratio ]1 trans-11 was the most dominant trans 18[ratio ]1 isomer in milk fat. In the first experiment, the proportions of the 18[ratio ]1 trans-11 isomer and conjugated linoleic acid (CLA, 18[ratio ]2 cis-9, trans-11) were highest for the pasture-only diets, and significantly (P<0·05) decreased with grain supplementation. The opposite result was observed in the second experiment, conducted in a different dairy region, suggesting that factors such as the quality of pasture on offer and the physiological state of the cow could affect the content of CLA and trans fatty acids in milk fat. In both experiments, there was a significant positive linear relationship between CLA and 18[ratio ]1 trans-11. Fibre supplements had little effect on the fatty acid composition of the milk.

scholarly journals Feeding Polyunsaturated Supplements to Grazing Dairy Cows Improve the Healthy Value of Milk Fatty Acids

2017 ◽  
Vol 08 (08) ◽  
pp. 759-782 ◽  
Author(s):  
Gerardo Antonio Gagliostro ◽  
Daniel Alberto Garciarena ◽  
María Alejandra Rodriguez ◽  
Liliana Elisabet Antonacci
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Fatty Acids ◽  
Grazing Dairy Cows

scholarly journals Effect of circulating glucagon and free fatty acids on hepatic FGF21 production in dairy cows

2017 ◽  
Vol 313 (5) ◽  
pp. R526-R534 ◽  
Author(s):  
Luciano S. Caixeta ◽  
Sarah L. Giesy ◽  
Christopher S. Krumm ◽  
James W. Perfield ◽  
Anthony Butterfield ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Energy Demand ◽  
Fibroblast Growth Factor 21 ◽  
Early Lactation ◽  
Fibroblast Growth ◽  
Plasma Nefa ◽  
Nonesterified Fatty Acids ◽  
Relative Contribution ◽  
Key Factor

Modern dairy cows meet the energy demand of early lactation by calling on hormonally driven mechanisms to increase the use of lipid reserves. In this context, we recently reported that fibroblast growth factor-21 (FGF21), a hormone required for efficient use of lipid reserves in rodents, is upregulated in periparturient dairy cows. Increased plasma FGF21 in early lactation coincides with elevated circulating concentrations of glucagon (GCG) and nonesterified fatty acids (NEFA). To assess the relative contribution of these factors in regulating FGF21, two experiments were performed in energy-sufficient, nonpregnant, nonlactating dairy cows. In the first study, cows were injected with saline or GCG every 8 h over a 72-h period. GCG increased hepatic FGF21 mRNA by an average of fivefold over matched controls but had no effect on plasma FGF21. In the second study, cows were infused and injected with saline, infused with Intralipid and injected with saline, or infused with Intralipid and injected with GCG. Infusions and injections were administered intravenously over 16 h and subcutaneously every 8 h, respectively. Intralipid infusion increased plasma NEFA from 92 to 550 µM within 3 h and increased plasma FGF21 from 1.3 to >11 ng/ml 6 h later; FGF21 mRNA increased by 34-fold in liver but remained invariant in adipose tissue. GCG injections during the Intralipid infusion had no additional effects on plasma NEFA, liver FGF21 mRNA, or plasma FGF21. These data implicate plasma NEFA as a key factor triggering hepatic production and increased circulating concentrations of FGF21 in early lactation.

scholarly journals Relationship of Plasma Nonesterified Fatty Acids and Walking Activity in Postpartum Dairy Cows

2006 ◽  
Vol 89 (8) ◽  
pp. 2977-2979 ◽  
Author(s):  
A.A. Adewuyi ◽  
J.B. Roelofs ◽  
E. Gruys ◽  
M.J.M. Toussaint ◽  
F.J.C.M. van Eerdenburg
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Nonesterified Fatty Acids ◽  
Walking Activity ◽  
Relationship Of
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