Antigenic Crossreactivity and Lipopolysaccharide Neutralization Properties of Bovine Immunoglobulin G

ABSTRACT The gene (pomA) encoding PomA, an OmpA-like major outer membrane protein of the bovine respiratory pathogen Pasteurella haemolytica, was cloned, and its nucleotide sequence was determined. The deduced amino acid sequence of PomA has significant identity with the sequences of other OmpA family proteins. Absorption of three different bovine immune sera with whole P. haemolytica cells resulted in a reduction of bovine immunoglobulin G reactivity with recombinant PomA in Western immunoblots, suggesting the presence of antibodies against PomA surface domains.

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Passive immunization of hamsters against disease caused by Clostridium difficile by use of bovine immunoglobulin G concentrate.

Infection and Immunity ◽

10.1128/iai.59.6.2215-2218.1991 ◽

1991 ◽

Vol 59 (6) ◽

pp. 2215-2218 ◽

Cited By ~ 82

Author(s):

D M Lyerly ◽

E F Bostwick ◽

S B Binion ◽

T D Wilkins

Keyword(s):

Clostridium Difficile ◽

Immunoglobulin G ◽

Passive Immunization ◽

Bovine Immunoglobulin

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Incidence of Colostrum in Raw Milk

Journal of Food Protection ◽

10.4315/0362-028x-56.7.625 ◽

1993 ◽

Vol 56 (7) ◽

pp. 625-626 ◽

Cited By ~ 5

Author(s):

JERZY ZAWISTOWSKI ◽

RUFINA MACKINNON

Keyword(s):

Immunoglobulin G ◽

Raw Milk ◽

Radial Immunodiffusion ◽

Milk Samples ◽

Bovine Immunoglobulin

A survey was conducted to determine the colostrum content in raw milk from dairies in Manitoba, Canada. Colostrum was indirectly measured by the determination of bovine immunoglobulin G (IgG) using a radial immunodiffusion assay. The results showed that 360 milk samples, which accounted for 89% of the total tested samples, were contaminated with colostrum. Of these, 320 samples had IgG levels in the range of 1.0 to 1.5 mg/ml, while 38 samples had an IgG content in the range of 1.5 to 2.0 mg/ml. Two milk samples contained IgG in excess of 2 mg/ml.

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Determination of the Presence of Bovine Immunoglobulin G in Liquid or Spray-Dried Porcine Plasma and Whole Blood by Agar Gel Immunodiffusion

Journal of AOAC International ◽

10.1093/jaoac/87.1.78 ◽

2004 ◽

Vol 87 (1) ◽

pp. 78-82 ◽

Cited By ~ 3

Author(s):

Javier Polo ◽

Neus Saborido ◽

Jesús Ródenas ◽

Carmen Rodríguez

Keyword(s):

Immunoglobulin G ◽

Whole Blood ◽

Animal Feed ◽

Cross Contamination ◽

Agar Gel ◽

Laboratory Equipment ◽

Before And After ◽

Bovine Immunoglobulin ◽

Blood Centrifugation

Abstract There is currently urgent interest in identifying the species of origin of the components of different animal by-products. In Europe, this interest is expected to increase with authorization of the re-introduction of these proteins into animal feed formulations. The number of validated methods to differentiate the species of origin for most of these products is limited. An easy, inexpensive, and accurate test was developed to determine the cross-contamination of bovine blood or plasma in porcine whole blood and plasma, both before and after spray drying. Agar gel immunodiffusion (AGID), the studied technique, detected the presence of bovine immunoglobulin G (IgG) in porcine plasma and in whole blood at inclusion levels >0.5% (v/v) in all cases. However, detectability was lower in liquid plasma (0.3%, v/v) and in liquid whole blood (0.5%, v/v). No differences were found when cross-contamination was simulated before or after whole blood centrifugation. The method described is reliable and inexpensive, and the samples are easy to prepare. Both minimal laboratory equipment and expertise are required for detection of bovine IgG in porcine blood products at inclusion levels of >0.5% (v/v).

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Blood-tissue transport of exogenous albumin and immunoglobulin G in genetically analbuminemic rats

Journal of Applied Physiology ◽

10.1152/jappl.1993.74.2.559 ◽

1993 ◽

Vol 74 (2) ◽

pp. 559-566 ◽

Cited By ~ 8

Author(s):

E. M. Renkin ◽

V. L. Tucker ◽

H. Wiig ◽

G. Kaysen ◽

L. Sibley ◽

...

Keyword(s):

Adult Female ◽

Immunoglobulin G ◽

Skeletal Muscles ◽

Interstitial Fluid ◽

Venous Pressure ◽

Tracer Uptake ◽

Sprague Dawley ◽

Interstitial Fluid Volume ◽

Uptake Studies ◽

Bovine Immunoglobulin

Tracer uptake studies were carried out in adult female Nagase (NA) strain analbuminemic rats [derived from Sprague-Dawley (SD) stock] and in adult female SD controls to determine the extent to which capillary permeability to plasma proteins is altered in the absence of endogenous albumin. Accessory measurements (arterial pressure, central venous pressure, plasma and interstitial fluid protein concentrations and oncotic pressures, plasma volume, and interstitial fluid volume) confirm the report of Joles et al. [Am. J. Physiol. 257 (Renal Fluid Electrolyte Physiol. 26): F23-F28, 1989] that shows elevated plasma volumes, normal interstitial fluid volumes, nearly normal plasma oncotic pressures (due to elevated globulin concentrations), and lower interstitial fluid oncotic pressures. In skin, skeletal muscles, and heart muscle, clearances of exogenous heterologous (bovine) albumin were 20–40% higher in NA than in SD controls. In intestine, albumin clearances were 20–30% lower. In NA rats blood-to-tissue clearances of heterologous (bovine) immunoglobulin G in skin and heart were higher and in the intestine they were lower than in SD controls; however, clearances in skeletal muscles were not elevated. The differences between NA and SD are small compared with the large increases in macromolecular permeabilities reported by others for organs and single microvessels perfused with albumin-free fluids.(ABSTRACT TRUNCATED AT 250 WORDS)

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Bovine immunoglobulin G, β-lactoglobulin, α-lactalbumin and serum albumin in colostrum and milk during the early post partum period

Journal of Dairy Research ◽

10.1017/s0022029999003581 ◽

1999 ◽

Vol 66 (3) ◽

pp. 421-430 ◽

Cited By ~ 72

Author(s):

DIDIER LEVIEUX ◽

ALAIN OLLIER

Keyword(s):

Serum Albumin ◽

Immunoglobulin G ◽

Post Partum ◽

Whey Proteins ◽

Milk Supply ◽

Holstein Friesian ◽

The Mean ◽

Bovine Immunoglobulin ◽

Β Lactoglobulin ◽

Friesian Cows

Colostrum and milk samples from 60 Holstein–Friesian cows were analysed for concentrations and yields of immunoglobulin G (IgG), β-lactoglobulin (β-lg), α-lactalbumin (α-la) and serum albumin (BSA) throughout the first 16 milkings post partum (8 d of lactation) using a single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG 59·8±28·5, β-lg 14·3±4·6, α-la 2·04±0·6, BSA 1·21±0·44. Large variations were recorded for IgG concentrations (15·3–176·2 mg/ml) and yields (0·2–925 g). Cows in their first lactation produced significantly lower concentrations and yields of colostral IgG than cows in later lactations. A colostral yield of IgG below the 100 g required to prevent calf hypo-γ-globulinaemia was found in 18·3% of the cows. The concentrations of IgG, β-lg and BSA dropped abruptly in subsequent milkings and α-la concentration decreased slowly. The mean IgG concentration was <2 mg/ml after eight milkings and <1 mg/ml after fifteen milkings. However, IgG concentration did not differ significantly, at the 1% level, during milkings 11–15. The results were tabulated to make it possible to calculate the excess of whey proteins that would be obtained if early milks were illegally added to the milk supply.

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