scholarly journals 68 Effects of Plant Growth-Promoting Rhizobacteria on Fall-Stockpiled Bermudagrass

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 31-32
Author(s):  
Madison Cole ◽  
Megan E Griffin ◽  
Sandra L Dillard ◽  
Mary K Mullenix ◽  
Russ B Muntifering ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutritive Value ◽  
Plant Growth Promoting Rhizobacteria ◽  
N Fertilizer ◽  
Alternative Form ◽  
Forage Production ◽  
Synthetic Fertilizer ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Forage production practices have been greatly affected by the increasing cost of N fertilization. Therefore, supplemental and alternative N sources are needed to ensure the economic viability of these systems. A 2-yr, small plot study was designed to evaluate plant growth-promoting rhizobacteria (PGPR) as an alternative form of N fertilizer for fall-stockpiled bermudagrass (Cynodon dactylon). Eighteen 1-m2 ‘Coastal’ bermudagrass plots were treated with a synthetic N fertilizer, DH44 (PGPR strain), DH44+fertilizer, Blend 20 (PGPR blend), Blend 20+fertilizer, and a control, then stockpiled through the fall. Two PGPR applications were applied in late-August and again 30 d later. Fertilizer and PGPR+fertilizer plots received (NH4)2SO4, at a rate of 56 kg N/ha. One-third of each plot was clipped to 2.5 cm in November, December, and January, respectively. Forage DM yield, CP, NDF, ADF, and ADL were determined via wet chemistry at the Auburn University Ruminant Nutrition Laboratory (Auburn, AL). Data were analyzed using PROC MIXED (SAS 9.4, SAS Inst., Cary, NC) as a completely randomized design. Yield was greatest (P ≤ 0.0318) for Blend 20+fertilizer, but it was not different (P = 0.2552) from that of the synthetic fertilizer (1,914 kg ha-1, 1,768 kg ha-1, respectively). Concentration of CP was least (P ≤ 0.0437) for DH44 and Blend 20 treatments (90 g kg-1 and 92 g kg-1, respectively). Concentrations of NDF for the control were different (P ≤ 0.0045) for all treatments except synthetic fertilizer (P = 0.1092). Concentrations of ADF were not different (P ≥ 0.1613) excluding the control (P ≤ 0.0525; 342.8 g kg-1and 358.0 g kg-1, respectively). In vitro true digestibility (IVTD) was not different (P = 0.0947) among all treatments (463.1 g kg-1). All yield and nutritive value parameters were greater (P ≤ 0.0246) in Year 2. These results indicate that PGPR is a viable option for biofertilization of fall-stockpiled bermudagrass; however, further investigation into the effects of PGPR inoculants at a field scale are needed.

scholarly journals 146 Evaluation of plant growth promoting rhizobacteria on stockpiled bermudagrass

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 36-37
Author(s):  
Megan E Griffin ◽  
Mary K Mullenix ◽  
D W Held ◽  
Russ B Muntifering ◽  
Sandra L Dillard
Keyword(s):  
Plant Growth ◽  
Nutritive Value ◽  
Plant Growth Promoting Rhizobacteria ◽  
N Fertilization ◽  
Alternative Form ◽  
Forage Production ◽  
Commercial Fertilizer ◽  
Harvest Dates ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Plant growth-promoting rhizobacteria (PGPR) are non-pathogenic, soil-inhabiting, beneficial bacteria that colonize the roots of plants. Some PGPR strains are reported to increase nutrient uptake and fix atmospheric N, which suggests that biofertilization with PGPR may provide an alternative to N fertilization for forage production. In mid-August 2017, a study was initiated to evaluate PGPR as an alternative form of N fertilization for fall-stockpiled bermudagrass. Eighteen 1-m2plots were mowed to a 2.5-cm stubble height prior to stockpiling. Two strains of PGPR (Blend 20 and DH44) were selected for evaluation based on performance in greenhouse trials. Treatments included: control, fertilizer, DH44, DH44+fertilizer, Blend 20, and Blend 20+fertilizer (n = 3).Two applications of PGPR were applied at the beginning of the stockpiling season and 30 d later. Ammonium sulfate was applied at 56 kg/ha during the first PGPR application. Plots were clipped to a height of 2.5 cm in mid-November, December, and January to determine yield and nutritive value. Data were analyzed using PROC MIXED (SAS 9.4) as a completely randomized design.Yield was greater (P ≤ 0.007) for Blend 20+fertilizer, DH44, and Blend 20 (695, 673, and 664 kg DM/ha, respectively) than the control (598 kg DM/ha). Forage DM yield differed among harvest dates, with Blend 20+Fertilizer having the highest yield in January (835 kg DM/ha). Blend 20+fertilizer, control, and fertilizer treatments had the greatest effect on CP concentration (9.1, 9.5, and 10.1%, respectively). Concentrations of NDF and ADF were greatest (P ≤ 0.01) for Blend 20, Blend 20+fertilizer, DH44, and fertilizer. Percentage IVTD decreased with the later harvests (46.1, 33.8, and 39.0% in November, December, and January, respectively); however, CP was unchanged across all harvests (P3 0.12). Overall, PGPR increased DM yield of stockpiled bermudagrass while maintaining forage nutritive value similar to commercial fertilizer.

scholarly journals Forage Mass, Tillering, Nutritive Value and Root System of Ruzigrass Inoculated with Plant Growth Promoting Bacteria Associated with Doses of N-Fertilizer

2020 ◽  
Vol 8 (10) ◽  
pp. 41-55
Author(s):  
Artur Roque Domingues Barreiros ◽  
Ulysses Cecato ◽  
Camila Fernandes Domingues Duarte ◽  
Mariangela Hungria ◽  
Thiago Trento Biserra ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root System ◽  
Nutritive Value ◽  
Block Design ◽  
N Fertilizer ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

The aim of this study was evaluating the effect of the inoculation of plant growth promoting bacteria (PGPB) in forage mass, tillering, nutritive value and root system of ruzigrass (Urochloa ruziziensis (R. Germ. & Evrard) Crins (syn. of Brachiaria ruziziensis) associated with doses of N-fertilizer. The bacteria inoculated were Azospirillum brasilense Ab-V5, Pseudomonas fluorescens CCTB03 and Pantoea ananatis AMG 521, plus the control treatment (non-inoculated), associated with doses of N-fertilizer (0, 50 and 100 kg N ha-1). The experiment was performed in a randomized block design, in a 4x3 factorial scheme, with four replicates, totaling 48 plots (12 m2). There were no effects of the PGPB and the use of N-fertilizer on the leaf blade, stem+sheath, forage mass, daily and yearly accumulation of forage mass. The PGPB did not have influence on the density of tillers. The doses of 50 and 100 kg of N ha-1 increased the amount of tillers. The AMG 521 strain associated with N-fertilizer provided heavier tillers. There was no effect of the PGPB on crude protein (CP), neutral detergent fiber (NDF), as well as acid detergent fiber (ADF), and in vitro digestibility of the dry matter (IVDDM).  The use of 100 kg of N ha-1 contributed to an increase in CP and a decrease in NDF. The AMG 521 strain contributed to a smaller diameter of the root. Strains CCTB03 and AMG 521 demonstrated a smaller area, length and root density when associated with the dose of 50kg of N ha-1. In general, the PGPB were not efficient in promoting productive increments in ruzigrass.

scholarly journals Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

2021 ◽  
Vol 12 ◽  
Author(s):  
Maria Chiara Guerrieri ◽  
Andrea Fiorini ◽  
Elisabetta Fanfoni ◽  
Vincenzo Tabaglio ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Nutrition ◽  
Growth Regulation ◽  
Root Length Density ◽  
Plant Growth Promoting Rhizobacteria ◽  
Conventional Management ◽  
Tomato Field ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different PGPR strains can be differentiated by the presence of different genes. In this study we reported a comprehensive evaluation of a novel PGPR Klebsiella variicola UC4115 from the field to the lab, and from the lab to the plant. The isolate from tomato field was screened in-vitro for different activities related to plant nutrition and growth regulation as well as for antifungal traits. We performed a functional annotation of genes contributing to plant-beneficial functions previously tested in-vitro. Furthermore, the in-vitro characterization, the whole genome sequencing and annotation of K. variicola UC4115, were compared with the well-known PGPR Azospirillum brasilense strain Sp7. This novel comparative analysis revealed different accumulation of plant-beneficial functions contributing genes, and the presence of different genes that accomplished the same functions. Greenhouse assays on tomato seedlings from BBCH 11–12 to BBCH > 14 were performed under either organic or conventional management. In each of them, three PGPR inoculations (control, K. variicola UC4115, A. brasilense Sp7) were applied at either seed-, root-, and seed plus root level. Results confirmed the PGP potential of K. variicola UC4115; in particular, its high value potential as indole-3-acetic acid producer was observed in increasing of root length density and diameter class length parameters. While, in general, A. brasilense Sp7 had a greater effect on biomass, probably due to its high ability as nitrogen-fixing bacteria. For K. variicola UC4115, the most consistent data were noticed under organic management, with application at seed level. While, A. brasilense Sp7 showed the greatest performance under conventional management. Our data highlight the necessity to tailor the selected PGPR, with the mode of inoculation and the crop-soil combination.

scholarly journals Evaluation of diverse range microbes for their plant growth promoting abilities and their pesticide compatibility

2021 ◽  
pp. 47-58
Author(s):  
Tulja Sanam ◽  
S. Triveni ◽  
J. Satyanaryana ◽  
Sridhar Goud Nerella ◽  
K. Damodara Chari ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Atomic Absorption Spectrophotometry ◽  
In Vitro Assays ◽  
Bacterial Isolates ◽  
Diverse Range ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Bacillus Isolate

Plant growth-promoting rhizobacteria (PGPR) contribute to an increase in crop yield through an environmentally friendly method, therefore eight rhizospheric bacteria, two of each genera Bacillus, Pseudomonas, Azotobacter and Azospirillum were examined for their efficacy to solubilize mineral nutrients using atomic absorption spectrophotometry and a flame photometer. Their potency to produce phytohormones, synthesis biocontrol components and their compatibility with pesticides using in vitro assays was studied. All of the chosen bacterial isolates proved positive for the above-mentioned Plant Growth Promoting traits. Among the eight bacterial isolates Pseudomonas isolate P69 showed the highest phosphorous solubilization efficiency of 190.91 % and another isolate P48 produced a maximum of 27.63µg mL-1 of gibberellic acid, Bacillus isolate B120 could solubilize maximum amount of ZnO and ZnCO3 accounting for 21.3ppm and 25.9ppm, respectively, not merely in terms of solubilization when compared to the other isolates, B120 produced the highest levels of HCN (77.33 ppm TCC) and siderophores (48.87psu). On day 9 after inoculation, Azotobacter isolate AZB17 performed effectively in potassium solubilization of 6.25g mL-1 with a pH drop to 3.83. The Azospirillum isolate ASP25 outperformed all other isolates in terms of IAA production (22.64g mL-1) and Bacillus isolate B365 was found to be more compatible with eight different pesticides used in the field at varying concentrations. All of these factors point to the possibility of using these bacterial isolates B120, P48, P69, AZB17, and ASP25 as biofertilizers in sustainable agriculture.

scholarly journals Impacts of Plant Growth-Promoting Rhizobacteria on Tropical Forage Grass in Brazil

2020 ◽  
Vol 8 (1) ◽  
pp. 342
Author(s):  
Monyck Jeane dos Santos Lopes ◽  
Moacyr Bernardino Dias-Filho ◽  
Thomaz Henrique dos Reis Castro ◽  
Edilson Ferreira da Silva ◽  
Marcela Cristiane Ferreira Rêgo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Biomass Production ◽  
Nutritive Value ◽  
Plant Growth Promoting Rhizobacteria ◽  
Soluble Carbohydrates ◽  
Production Plant ◽  
Pasture Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

The aim of this study was to evaluate the effects of plant growth-promoting rhizobacteria effects on anatomical characteristics and nutritional value of Brachiaria (Syn. Urochloa) brizantha cv. BRS Piatã. The experimental design applied was completely randomized design  with three treatments: (1) non-inoculated unfertilized-control plants (C-), (2) non-inoculated fertilized-control plants (C+) and (3) B. brizantha inoculated with Pseudomonas fluorescens (BRM-32111) and Burkholderia pyrrocinia (BRM-32113). The following parameters were evaluated at 35 days after seedling emergence: biomass production, plant height, net photosynthesis (A), water-use efficiency (WUE), chlorophyll (SPAD), anatomical and nutritional. The rhizobacteria modified the anatomy of the leaf, culm and roots of B. brizantha. They also increased the chlorophyll content, A, WUE, total soluble carbohydrates, starch and crude protein contents, N, P, Mg and Fe concentrations, plant height, root area and biomass production. Therefore, we conclude that co-inoculation with P. fluorescens (BRM-32111) and B. pyrrocinia (BRM-32113) modified the anatomy and biochemistry of B. brizantha, promoting growth and nutrient accumulation. Therefore, these findings set up the basis for additional exploratory studies, using these rhizobacteria as biotechnological innovation with potential of use as biofertilizer in B. brizantha, aiming higher productivity and nutritive value in a more eco-friendly and sustainable pasture production system.

scholarly journals Isolation and screening of multifunctional rhizobacteria from the selected sites of Madhupur, Narshingdi and Mymensingh, Bangladesh

2015 ◽  
Vol 2 (1) ◽  
pp. 1-8
Author(s):  
Moonmoon Nahar Asha ◽  
Atiqur Rahman ◽  
Quazi Forhad Quadir ◽  
Md Shahinur Islam
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Plant Growth Promoting Rhizobacteria ◽  
N Fixation ◽  
National Botanical Research Institute ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Botanical Research ◽  
Plant Growth Promoting Traits

A laboratory experiment was performed to isolate some native rhizobacteria that could be used as bioinoculants for sustainable crop production. A total of 43 rhizobacteria were isolated from undisturbed plant rhizosphere soils of three different locations of Bangladesh and evaluated their plant growth promoting traits, both direct and indirect. The study has screened out isolates on the basis of their phosphorous solubilization and nitrogen (N) fixation. The phosphate solubilization assay in National Botanical Research Institute of Phosphate (NBRIP) medium revealed that 12 bacterial isolates were able to solubilize tricalcium phosphate and the rhizobacteria M25 showed best performance with a PSI of 3.33 at 5 day. Exactly 47% (20 isolates) of the isolated rhizobacteria were able to grow in N-free Winogradsky’s medium, which is an indication of potential N2-fixers. Among the 20 potential N-fixers, 15 were able to grow within 24 hours of incubation indicating that they are more efficient in Nfixation. The present study successfully isolated and characterized 43 rhizobacteria. Some of these isolated rhizobacteria have potential plant growth promoting traits and are potential plant growth promoting rhizobacteria (PGPR) candidate. Considering all plant growth promoting traits, the isolate F37 was the best followed by M6. However, further experiments are needed to determine the effectiveness of these isolates under in vitro and different field conditions to understand the nature of interaction with the plant and environment.Res. Agric., Livest. Fish.2(1): 1-8, April 2015

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