scholarly journals PCR multiplex en tiempo real para sepsis (Film array): caracterización como método diagnóstico en un centro pediátrico en Cali

2020 ◽  
Vol 47 (2) ◽  
pp. 69-73
Author(s):  
Maria Eugenia Cuastumal ◽  
Maria Isabel Rodríguez ◽  
Luis Fernando Mejía Rivera ◽  
José Gómez Urrego
Keyword(s):  
Molecular Film ◽  
Pcr Multiplex

La sepsis es una condición que genera alta morbilidad y mortalidad en donde la detección temprana de los microorganismos causantes es esencial para un manejo oportuno. Objetivo: determinar la frecuencia de detección y tipificación de los microorganismos por el PCR multiplex (Film Array) en pacientes pediátricos (menores de 18 años) ingresados al servicio de urgencias con el diagnóstico confirmado de sepsis. Materiales y Métodos: Se realizó un estudio observacional descriptivo retrospectivo. Fueron analizados los paneles positivos de hemocutivos de pacientes menores de 18 años, con diagnóstico de sepsis, por medio de la tecnología PCR multiplex (Film Array) en el Hospital Clínica Infantil Club Noel. Los resultados fueron comparados. Los datos fueron analizados con Microsof Excel, empleando estadísticas descriptivas. Resultados: De 101 hemocultivos positivos, el panel molecular Film Array detecto el 92 (91%). Hubo predominio de sepsis con puerta de entrada respiratoria y menores de 3 años de edad. Los gérmenes más frecuentemente detectados fueron los gram negativos tanto en el hemocultivo como en el panel molecular. Conclusiones: los hallazgos en cuanto a detección de bacteriemia, en nuestra población por PCR multiplex en tiempo real demuestran su capacidad de detección y su importancia en el diagnóstico temprano y tratamiento oportuno. Correspondencia: Maria Eugenia Cuastumal. Correo: [email protected] Conflicto de interés: Los autores declaran no poseer conflicto de interés. Recibido: 14/12/19 Aceptado:11/06/2020

An Analysis of Dissociation of Molecules in a High Resolution Electron Microscope

1973 ◽  
Vol 31 ◽  
pp. 486-487
Author(s):  
Mihir Parikh
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Cross Sections ◽  
Resolving Power ◽  
Peak Intensity ◽  
Molecular Film ◽  
Resolution Electron ◽  
Dissociation Cross Section ◽  
High Resolution Electron Microscope ◽  
The Stability

It is well known that the resolution of bio-molecules in a high resolution electron microscope depends not just on the physical resolving power of the instrument, but also on the stability of these molecules under the electron beam. Experimentally, the damage to the bio-molecules is commo ly monitored by the decrease in the intensity of the diffraction pattern, or more quantitatively by the decrease in the peaks of an energy loss spectrum. In the latter case the exposure, EC, to decrease the peak intensity from IO to I’O can be related to the molecular dissociation cross-section, σD, by EC = ℓn(IO /I’O) /ℓD. Qu ntitative data on damage cross-sections are just being reported, However, the microscopist needs to know the explicit dependence of damage on: (1) the molecular properties, (2) the density and characteristics of the molecular film and that of the support film, if any, (3) the temperature of the molecular film and (4) certain characteristics of the electron microscope used

Study on the Mechanism of Enhancing Oil Recovery by Molecular Film Displacement

2011 ◽  
Vol 236-238 ◽  
pp. 2135-2141
Author(s):  
Qi Cheng Liu ◽  
Yong Jian Liu
Keyword(s):  
Oil Recovery ◽  
Electrostatic Interaction ◽  
Ultrathin Films ◽  
Water Solution ◽  
Wettability Alteration ◽  
Nanometer Scale ◽  
Rock Surface ◽  
Capillary Imbibition ◽  
Molecular Film ◽  
Polymer Surfactant

Molecular film displacement is a new nanofilm EOR technique. A large number of experiments show that the mechanism of molecular film displacement is different from conventional chemical displacement (polymer, surfactant, alkali and ASP displacement etc). With water solution acting as transfer medium, molecules of the filming agent develop the force to form films through electrostatic interaction, with efficient molecules deposited on the negatively charged rock surface to form ultrathin films at nanometer scale. This change the properties of reservoir surface and the interaction condition with crude oil, making the oil easily be displaced as the pores swept by the injected fluid. Thus oil recovery is enhanced. The mechanism of molecular filming agent mainly includes absorption, wettability alteration, diffusion and capillary imbibition etc.

scholarly journals 5 Rapid screening for large rearrangement by a 2 PCR-multiplex strategy

2006 ◽  
Vol 5 ◽  
pp. S2
Author(s):  
M.P. Audrezet ◽  
K. Giteau ◽  
O. Raguenes ◽  
M.N. Kervennic ◽  
C. Ferec
Keyword(s):  
Rapid Screening ◽  
Large Rearrangement ◽  
Pcr Multiplex

Formation and Structure of Inhibitive Molecular Film of Oxadiazole on Iron Surface

2017 ◽  
Vol 121 (39) ◽  
pp. 21420-21429 ◽  
Author(s):  
Qi Zheng ◽  
Jinyang Jiang ◽  
Dongshuai Hou ◽  
Shengping Wu ◽  
Fengjuan Wang ◽  
...  
Keyword(s):  
Iron Surface ◽  
Molecular Film

scholarly journals Molecular and morphological approaches for species delimitation and hybridization investigations of two Cichla species

2017 ◽  
Vol 107 (0) ◽  
Author(s):  
Andrea A. F. Mourão ◽  
Diogo Freitas-Souza ◽  
Diogo T. Hashimoto ◽  
Daniela C. Ferreira ◽  
Fernanda D. do Prado ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Parental Species ◽  
Future Research ◽  
Correct Identification ◽  
Pcr Multiplex ◽  
Pcr Rflp ◽  
Genetic Pattern ◽  
Morphometric Methods ◽  
Species Specific ◽  
Specific Nucleotide

ABSTRACT The hybridization is a widely-discussed issue in several studies with fish species. For some authors, hybridization may be related with diversification and speciation of several groups, or also with the extinction of populations or species. Difficulties to differentiate species and hybrids may be a problem to correctly apply a management of wild species, because hybrid lineages, especially the advanced ones, may resemble the parental species. The genus Cichla Bloch & Schneider, 1801 constitutes an interesting experimental model, considering that hybridization and taxonomic uncertainties hinder a correct identification. Considering these problems, in this study, we developed genetic methodologies and applied meristic and morphometric approaches in wild samples in order to identify species and for test a possible hybridization between Cichla kelberi Kullander & Ferreira, 2006 and Cichla piquiti Kullander & Ferreira, 2006. For this, C. kelberi, C. piquiti and potential hybrid ( carijó) individuals were collected in Paraná and Tietê rivers (SP, Brazil). For meristic and morphometric methods, the individuals were analyzed using the statistical software Pcord 5:31, while for molecular methods, primers for PCR-multiplex were designed and enzyme for PCR-RFLP were selected, under the species-specific nucleotide. All results indicated that the carijó is not an interspecific hybrid, because it presented identical genetic pattern and morphology closed to C. piquiti. Thus, we propose that carijó is a C. piquiti morphotype. In addition, this study promotes a new molecular tool that could be used in future research, monitoring and management programs of the genus Cichla.

Molecular dynamics method in studies of molecular film growth processes

2006 ◽  
Vol 47 (3) ◽  
pp. 532-548 ◽  
Author(s):  
I. F. Golovnev ◽  
T. V. Basova ◽  
E. K. Koltsov ◽  
I. K. Igumenov
Keyword(s):  
Molecular Dynamics ◽  
Film Growth ◽  
Molecular Dynamics Method ◽  
Growth Processes ◽  
Molecular Film ◽  
Dynamics Method

Pressure-Sensitive Molecular Film

2015 ◽  
pp. 2871-2879
Author(s):  
Yu Matsuda ◽  
Hiroki Yamaguchi ◽  
Tomohide Niimi
Keyword(s):  
Pressure Sensitive ◽  
Molecular Film

scholarly journals Avanços em testes genéticos pré-implantacionais: revisão de literatura

2021 ◽  
Vol 10 (15) ◽  
pp. e429101523103
Author(s):  
Beatriz de Araujo Scapin Scapin ◽  
Catherine Caldas de Mesquita Mesquita ◽  
Rodrigo Tenório Padilha Padilha ◽  
Deborah de Melo Magalhães Padilha Padilha
Keyword(s):  
Multiplex Pcr ◽  
Nested Pcr ◽  
Ion Torrent ◽  
Pcr Multiplex

O desenvolvimento das tecnologias genéticas pré-implantacionais nos permite, hoje, identificar anomalias genéticas hereditárias, realizar triagem em busca de mutações e analisar previamente a viabilidade de um embrião, inclusive conhecer a compatibilidade genética embrionária com a de uma pessoa já nascida e doente, que necessite de um doador compatível. Os testes genéticos pré-implantacionais consistem em um conjunto de técnicas, realizadas após o processo de Fertilização in vitro (FIV) e antes da implantação do embrião. Devido à escassez de artigos na literatura brasileira sobre o assunto, foi desenvolvida uma revisão de literatura, que tem como objetivo explanar sobre os avanços que ocorreram nos últimos 30 anos nos testes utilizados para o diagnóstico genético pré-implantacional (DGPI), esclarecendo e apresentando seus protocolos. Para isso, foram selecionadas as principais técnicas adotadas, sendo elas Nested PCR, Multiplex PCR, qPCR, FISH, aCGH, SNP’s, NGS Ion Torrent e Illumina, além de contextualizar a cronologia dos testes e apresentar todo o contexto ético que os envolve. Para tanto, foram utilizadas 48 referências de língua inglesa, portuguesa e espanhola, em sua maioria datadas entre 2017 a 2021. É fato que o DGPI confere um grande avanço no campo da reprodução assistida e possibilita mulheres com idade avançada ou histórico recorrente de aborto, além de pessoas com anomalias genéticas, terem o DNA de seus embriões analisados antes de sua implantação, permitindo identificar mutações que poderão afetar a saúde do bebê e garantindo a sua compatibilidade com a vida.

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