EFFECT OF AMMONIUM PYRROLIDINE DITHIOCARBAMBATE ON THE FORMATION OF RACTIVE OXYGEN AND NITROGEN SPECIES IN LIVER OF RATS KEPT ON CARBOHYDRATE-LIPID DIET AND EXPOSED TO ROUND-THE-CLOCK LIGHTING

This aim of the experiment performed on 21 white rats is to clear up the effect of the nuclear factor kappa B (NF-kappa B) inhibitor on the production of reactive oxygen and nitrogen species in the liver of animals kept on carbohydrate-lipid diet and exposed to round-the-clock lighting. The study has demonstrated the administration of ammonium pyrrolidine diothiocarbamate (76 mg/kg three times a week, starting on the 30th day of the experiment) under the conditions of high-calorie carbohydrate-lipid diet (60 days) and exposure of the test animals to round-the-clock light (1500 lux for the last 30 days of the experiment) reduces the production of superoxide anion radical in the liver tissues by NADPH- and NADH-dependent electron transport chains by 45.2% and 43.5%. The production of this radical by leukocytes by NADPH oxidase was 41.6% lower than in the comparison group. The activity of NO-synthase (total and its inducible isoenzyme) decreased by 34.4 and 29.8%, the activity of the constitutive isoform increased threefold. The coupling index of the latter elevated by 5.16 times that indicates the restoration of the coupling state of this isoenzyme. The decrease in the generation of superoxide anion radical and nitric oxide was accompanied by lowering in the peroxynitrites concentration by 36.4%. We can conclude that the administration of ammonium pyrrolidine dithiocarbamate, an NF-kappa B inhibitor, during the metabolic syndrome simulated in rats by round-the-clock lightening exposure and diet rich in carbohydrates and fats is an effective means to restrict the production of reactive oxygen species.