Development And Validation Solvodin Tablet, Which Contains Bromhexine Hcl, By Rp-Hplc Technique Analysis

2020 ◽  
Vol 12 (sp1) ◽  
Keyword(s):  
Rp Hplc ◽  
Hplc Technique ◽  
Development And Validation ◽  
Technique Analysis

scholarly journals Method Development and Validation of Fludrocortisone Acetate Tablets by Reverse Phase HPLC Method

2020 ◽  
Vol 11 (4) ◽  
pp. 6826-6833
Author(s):  
Vijey Aanandhi M ◽  
Elancheziyan K ◽  
Yamini R ◽  
Prakash Chand T ◽  
Aysha Jadeera K A ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Mobile Phase ◽  
Method Development ◽  
Hplc Method ◽  
Reverse Phase Hplc ◽  
Rp Hplc ◽  
Method Development And Validation ◽  
Hplc Technique ◽  
Fludrocortisone Acetate ◽  
Development And Validation

The purpose or intent of this current study was to establish a fast and sensitive HPLC technique for the perseverance of Fludrocortisone acetate and utilizing best frequently used HPLC technique. This method had been validated as per the ICH requirements  to assure that the method consistently meets the predetermined specifications and quality attributes.Utilizing filtered and degassed pH 3.0 Phosphate buffer and Acetonitrile in the ratio 90:10 as a Mobile phase-A and pH 3.0 Phosphate buffer and Acetonitrile in the ratio 65:35 as a Mobile phase-B the established RP-HPLC technique was done. The separation was achieved by using Waters, X-Bridge Shield RP18, (150 X 4.6-mm), 3.5-µm column. Run time and Flow rate was set 45minutes and 1.2mL/min. Injection volume 100µL and wavelength was set 240nm.The correlation coefficient square for fludrocortisones acetate and Fludrocortisone Impurity was found to be 0.9991 and 0.99997. The SD and %RSD for Fludrocortisone Impurity was found to be 0.02 and 1.48 represents method precision. Following validated parameters lies within the limit. Hence, the developed method was precise, simple, fast and accurate.

scholarly journals Development and Validation of a Stability-Indicating HPLC Technique for Measuring Temozolomide in its Pharmaceutical Dose Form

2021 ◽  
Vol 37 (4) ◽  
pp. 900-904
Author(s):  
Narayan Shrivas ◽  
S. K. Mishra
Keyword(s):  
Mobile Phase ◽  
Stability Testing ◽  
Intermediate Precision ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Percent Recovery ◽  
Validation Parameters ◽  
Hplc Technique ◽  
Development And Validation ◽  
Dose Form

Using RP-HPLC, an accurate and precise technique for the measurement of Temozolomide in its pharmaceutical dose form was developed and validated. Chromatographic separation was achieved on an X Terra RP 18(250mm x 4.6mm), 5 µ column using a mobile phase consisting of methanol and buffer in the ratio of 10:990v/v. The flow rate was 1mL/min with the detection wavelength of 254 nm and retention time was found to be 20 min. The developed method was validated according to ICH guidelines. With a correlation coefficient of 0.9990, linearity was observed in the range of 50-150 percent. The %RSD of the developed method for method precision and Intermediate precision was found to be 0.65 % and 0.59 % respectively. With a percent recovery of 99.82 ±0.045, the approach was confirmed to be reliable. All of the validation parameters yielded results that were within acceptable limits. It was discovered that the procedure was accurate, exact, specific, rugged, and durable. As a result, the newly discovered approach can be used for finish product of quality control and stability testing on a regular basis and it has been confirmed to be stable for Temozolomide is available in both pure and pharmaceutical dose forms.

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