Differential wheat cultivars to discriminate pathogenicity of Japanese wheat yellow mosaic virus (WYMV) isolates.

Wheat yellow mosaic virus (WYMV) belongs to the genus Bymovirus in the family Potyviridae and has a bipartite genome (RNA1 and RNA2). WYMV in Japan is classified into three pathotypes (I to III) based on its pathogenicity to wheat cultivars. Among these three, pathotypes I and II are discriminated by their pathogenicity to the wheat cultivar Fukuho; pathotype I infects Fukuho but pathotype II does not. In the present study, the genomic regions that are involved in such pathogenicity were examined using infectious viral cDNA clones of pathotypes I and II. Reassortant experiments between viral RNA1 and RNA2 revealed the presence of a viral factor related to pathogenicity in RNA1. A chimeric pathotype II virus harboring a cylindrical inclusion (CI) cistron from pathotype I facilitated systemic infection of Fukuho, indicating that CI protein is involved in pathogenicity. Furthermore, analysis of chimeric and site-directed mutants revealed that three amino acids at the N-terminal region of CI protein were involved in pathogenicity to Fukuho. On the other hand, at the single-cell level, pathotype II replicated in protoplasts of Fukuho similar to that of pathotype I virus. These data suggest that differential pathogenicity between pathotypes I and II was considered to depend on the ability of cell-to-cell or long-distance viral movement, in which CI protein is involved. To the best of our knowledge, this is the first report to show the involvement of the bymoviral CI protein in pathogenicity.

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Genome-Wide Identification and Expression Analysis of the Histone Deacetylase Gene Family in Wheat (Triticum aestivum L.)

Plants ◽

10.3390/plants10010019 ◽

2020 ◽

Vol 10 (1) ◽

pp. 19

Author(s):

Peng Jin ◽

Shiqi Gao ◽

Long He ◽

Miaoze Xu ◽

Tianye Zhang ◽

...

Keyword(s):

Abiotic Stress ◽

Gene Family ◽

Mosaic Virus ◽

Stress Responses ◽

Viral Infections ◽

Phylogenetic Analyses ◽

Plant Viruses ◽

Gene Family Evolution ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus

Histone acetylation is a dynamic modification process co-regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Although HDACs play vital roles in abiotic or biotic stress responses, their members in Triticumaestivum and their response to plant viruses remain unknown. Here, we identified and characterized 49 T. aestivumHDACs (TaHDACs) at the whole-genome level. Based on phylogenetic analyses, TaHDACs could be divided into 5 clades, and their protein spatial structure was integral and conserved. Chromosomal location and synteny analyses showed that TaHDACs were widely distributed on wheat chromosomes, and gene duplication has accelerated the TaHDAC gene family evolution. The cis-acting element analysis indicated that TaHDACs were involved in hormone response, light response, abiotic stress, growth, and development. Heatmaps analysis of RNA-sequencing data showed that TaHDAC genes were involved in biotic or abiotic stress response. Selected TaHDACs were differentially expressed in diverse tissues or under varying temperature conditions. All selected TaHDACs were significantly upregulated following infection with the barley stripe mosaic virus (BSMV), Chinese wheat mosaic virus (CWMV), and wheat yellow mosaic virus (WYMV), suggesting their involvement in response to viral infections. Furthermore, TaSRT1-silenced contributed to increasing wheat resistance against CWMV infection. In summary, these findings could help deepen the understanding of the structure and characteristics of the HDAC gene family in wheat and lay the foundation for exploring the function of TaHDACs in plants resistant to viral infections.

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Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa

Theoretical and Applied Genetics ◽

10.1007/s00122-013-2181-y ◽

2013 ◽

Vol 126 (12) ◽

pp. 2921-2930 ◽

Cited By ~ 30

Author(s):

Renhui Zhao ◽

Haiyan Wang ◽

Jin Xiao ◽

Tongde Bie ◽

Shunhe Cheng ◽

...

Keyword(s):

Mosaic Virus ◽

Resistance Gene ◽

Virus Resistance ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus ◽

Haynaldia Villosa ◽

Virus Resistance Gene

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Breeding of a hard white wheat “Tamaizumi R” with resistance to wheat yellow mosaic virus

Breeding Research ◽

10.1270/jsbbr.18j19 ◽

2019 ◽

Vol 21 (1) ◽

pp. 35-40 ◽

Cited By ~ 2

Author(s):

Chikako Kiribuchi-Otobe ◽

Masaya Fujita ◽

Toshiyuki Takayama ◽

Hisayo Kojima ◽

Makiko Chono ◽

...

Keyword(s):

Mosaic Virus ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus ◽

White Wheat ◽

Hard White Wheat

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Endoplasmic reticulum remodeling induced by Wheat yellow mosaic virus infection studied by transmission electron microscopy

Micron ◽

10.1016/j.micron.2019.01.007 ◽

2019 ◽

Vol 120 ◽

pp. 80-90 ◽

Cited By ~ 1

Author(s):

Li Xie ◽

Xi-jiao Song ◽

Zhen-feng Liao ◽

Bin Wu ◽

Jian Yang ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Endoplasmic Reticulum ◽

Virus Infection ◽

Mosaic Virus ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus ◽

Transmission Electron ◽

Mosaic Virus Infection

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Wheat Yellow Mosaic Virus NIb Interacting with Host Light Induced Protein (LIP) Facilitates Its Infection through Perturbing the Abscisic Acid Pathway in Wheat

Biology ◽

10.3390/biology8040080 ◽

2019 ◽

Vol 8 (4) ◽

pp. 80 ◽

Cited By ~ 4

Author(s):

Zhang ◽

Liu ◽

Zhong ◽

Zhang ◽

Xu ◽

...

Keyword(s):

Abscisic Acid ◽

Mosaic Virus ◽

Distribution Patterns ◽

Host Factors ◽

Bimolecular Fluorescence Complementation ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus ◽

Positive Sense ◽

Acid Pathway ◽

Coding Capacity

Positive-sense RNA viruses have a small genome with very limited coding capacity and are highly reliant on host factors to fulfill their infection. However, few host factors have been identified to participate in wheat yellow mosaic virus (WYMV) infection. Here, we demonstrate that wheat (Triticum aestivum) light-induced protein (TaLIP) interacts with the WYMV nuclear inclusion b protein (NIb). A bimolecular fluorescence complementation (BIFC) assay displayed that the subcellular distribution patterns of TaLIP were altered by NIb in Nicotiana benthamiana. Transcription of TaLIP was significantly decreased by WYMV infection and TaLIP-silencing wheat plants displayed more susceptibility to WYMV in comparison with the control plants, suggesting that knockdown of TaLIP impaired host resistance. Moreover, the transcription level of TaLIP was induced by exogenous abscisic acid (ABA) stimuli in wheat, while knockdown of TaLIP significantly repressed the expression of ABA-related genes such as wheat abscisic acid insensitive 5 (TaABI5), abscisic acid insensitive 8 (TaABI8), pyrabatin resistance 1-Llike (TaPYL1), and pyrabatin resistance 3-Llike (TaPYL3). Collectively, our results suggest that the interaction of NIb with TaLIP facilitated the virus infection possibly by disturbing the ABA signaling pathway in wheat.

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