Portulaca oleracea aggregate (Portulacaceae) from the Chernivtsi Region (Ukraine)

2020 ◽  
Vol 12 (2) ◽  
pp. 251-262
Author(s):  
Olena Bulakh ◽  
Olena Volutsa ◽  
Alla Tokaryuk ◽  
Vasyl Budzhak ◽  
Ksenia Korzhan ◽  
...  

At the results of investigation of micromorphology features of seed surface of the Portulaca oleracea aggr. (Portulacaceae Juss.) from Chernivtsi Region (Ukraine) six morphotypes of this taxa were recorded. Some peculiarities of the seed morphology of Portulaca L., and features of the ultrastructure of seed surface of the P. oleracea aggr. morphotypes, based on the results of micromorphological studies with using electronic scanning microscope was described. The general for the representative of the genus (e.g. globous, ovate or almost reniform shaped of seeds; black or dark brown color) and specify for the morphological types (star-shaped, isodiametric or elongate cells of seed surface with central part and long or short rays; smooth surface of seed or with knobs (from 0,03 mm in diameter) or papillae (0,01–0,02 mm), etc.) peculiarities are determined. The original scanning electron microscopy (SEM) photographs of seed surface ultrastructure of the studied morphological types and key fragment for its determination are suggested. According to our investigation six morphotypes of Portulaca oleracea aggr. are presented in Chernivtsi Region. Some of them are new for studied territory (P. granulatostellulata (Poelln.) Ricceri & Arrigoni, P. papillatostellulata (Danin & H.G. Baker) Danin), and other (Portulaca nitida (Danin & H.G. Baker) Ricceri & Arrigoni, P. trituberculata Danin, Domina & Raimondo, P. tuberculata (Danin & H.G. Baker) Danin) – new floristic records for Ukraine, and one of them (P. oleracea) – was known before also. The maps of distribution of this morphotypes in the region are presented. At the results of morphotypes distribution the most common is P. granulatostellulata and P. oleracea – rare in studied region like as in Europe also.

Phytotaxa ◽  
2018 ◽  
Vol 336 (3) ◽  
pp. 263
Author(s):  
İLKER GENÇ ◽  
ŞÜKRAN KÜLTÜR

A comprehensive study based on seed morphology of perennial Euphorbia (Euphorbiaceae) sect. Pithyusa species occurring in Turkey is presented. A total of 14 species were studied. Seed characteristics were examined using scanning electron microscopy (SEM) as well as dissecting light microscopy. Significant features are: seed size, seed shape, shape of caruncle and seed surface ornamentation. Three different seed surface types (smooth, pitted, and wrinkled) were observed. Four main seed shapes (ovoid, oblong, quadrangular, and globose), as well as seven types of seed coat ornamentation (reticulate-areolate, areolate, alveolate, falsifoveate, pusticulate, colliculate and smooth) were found. The number of testa cells per 100 µm2 and also its range, from 8–12 to 57–63, are given.


2019 ◽  
Vol 11 (1) ◽  
pp. 87-92
Author(s):  
O Bulakh ◽  
V Protopopova ◽  
M Shevera

The results of studying of seeds of the Portulaca oleracea L. (Portulacaceae Juss.) from Transcarpathia (Ukraine) are presented in the publication. The seed surface ultrastructure of taxa of the P. oleracea-aggr. based on the results of micromorphological studies with using electronic scanning microscope was described. The general for the representative of the genus (e.g. globous, ovate or almost reniform shaped of seeds; dark) and specify for the morphological types (star-shaped, isodiametric or elongate cells of seed surface with central part and long or short rays; smooth surface of seed or with knobs (from 0,03 mm in diameter) or papillae (0,01–0,02 mm in diameter), etc.) peculiarity are determined. The original figures of seed surface ultrastructure of the studied morphological types and key for its determination are prepared. As a results of investigation the new for the Ukrainian flora taxa, P. cypria Danin, P. granulatostellulata (Poelln.) Ricceri & Arrigoni and P. papillatostellulata (Danin & H.G. Baker) Danin, from Transcarpathia are presented. All studied species are: kenophytes according time of immigration; xenophytes, according way of distribution; and ephemerophytes, according degree of naturalization. The map of distribution of the taxa in the region is presented.


2001 ◽  
Vol 7 (6) ◽  
pp. 526-529
Author(s):  
T. Guha ◽  
R. Bhar ◽  
V. Ganesan ◽  
A. Sen ◽  
R.L. Brahmachary

AbstractBoth scanning electron microscopy (SEM) and contact mode imaging via atomic force microscopy (AFM) have been utilized to elucidate the ultrastructure of mung bean seed surfaces. The results indicate: 1) that AFM is useful in the examination of seed surface ultrastructure ex-vaccuo without the need for additional complex preparative procedures; and 2) that both the cotyledon and seed coat of different strains of mung beans bear specific ultrastructural details unique to each strain. To our knowledge, these are the first AFM images of seed surfaces.


2015 ◽  
Vol 33 (4) ◽  
pp. 699-705 ◽  
Author(s):  
M. ÖZKAN ◽  
E. AKTOKLU ◽  
C. ÖZDEMIR

ABSTRACT The seed (nutlet) morphology of four Onobrychis Miller (Fabaceae: subfamily Papilionoideae, Section Hymenobrychis DC.) taxa from Turkey, including three endemic taxa, was examined using scanning electron microscopy. Onobrychis tournefortii, O. galegifolia, O. cappadocica, O. albiflora. The seed examined exhibited variation in size, shape, colour, and surface sculpturing. Seed size ranged between 4.0-5.2 mm length and 2.0-3.6 mm width. Observed shapes included; ellipticus anguste asymmtricus reniformis, Ovatus anguste asymmetricus reniformis and ellipticus reniformis. Seed surface sculpturing revealed two distinct types: reticulate and rugulate. Species of Onobrychisare generally similar and confused with those of Hedysarumduring the identification process. Seed surface micromorphology can suggest taxonomical diagnostic characters for distinguishing species. Many of these characteristics are diagnostic at both the generic and specific levels.


Author(s):  
Yasushi Kokubo ◽  
Hirotami Koike ◽  
Teruo Someya

One of the advantages of scanning electron microscopy is the capability for processing the image contrast, i.e., the image processing technique. Crewe et al were the first to apply this technique to a field emission scanning microscope and show images of individual atoms. They obtained a contrast which depended exclusively on the atomic numbers of specimen elements (Zcontrast), by displaying the images treated with the intensity ratio of elastically scattered to inelastically scattered electrons. The elastic scattering electrons were extracted by a solid detector and inelastic scattering electrons by an energy analyzer. We noted, however, that there is a possibility of the same contrast being obtained only by using an annular-type solid detector consisting of multiple concentric detector elements.


Zootaxa ◽  
2011 ◽  
Vol 2828 (1) ◽  
pp. 1 ◽  
Author(s):  
N. FERRETTI ◽  
G. POMPOZZI ◽  
F. PÉREZ-MILES

Three species of Grammostola (Araneae, Mygalomorphae, Theraphosidae) from central Argentina are redescribed and illustrated and their geographic distributions are reported. A new synonymy between G. vachoni Schiapelli & Gerschman 1961 and G. schulzei (Schmidt 1994) n. syn. is proposed. We did not find zones of co-occurrence with the three species but, in several localities, we found two Grammostola species in sympatry. The surface ultrastructure of three types of coxal setae was studied by scanning electron microscopy (SEM). Two morphological types of spiniform setae were found on the coxae. The first type consisted of short and strong piriform setae, with the surface completely covered by ridges, and the second type consisted of long and thinner spiniform setae, with a piriform basal portion. The basal portion of the setae had ridges, the central portion was smooth and the apical zone had numerous short barbs with curved tips. The different types of setae found in these Grammostola species are clearly distinguished, and could be new useful character to diagnose at least these species. In combination with other characteristics, these characters could probably help to solve the problems in other species of this difficult genus if not also other genera.


2005 ◽  
Vol 53 (2) ◽  
pp. 205-212 ◽  
Author(s):  
Soraya Naem

Scanning electron microscopy (SEM) was used to study the surface ultrastructure of female Thelazia lacrymalis obtained from naturally infected cattle. At the anterior end, the buccal capsule was cup shaped. The mouth was without lips and was surrounded by four cephalic papillae and two amphids. There were two lateral cervical papillae. Also, a single small papilla was seen on the ventral side, close to the vulvar opening. The vulva was located posterior to the end of the oesophagus and the tail carried an anal pore and a pair of phasmids near the tip. The cuticle was transversally annulated with fine striations.


2014 ◽  
Vol 35 (1) ◽  
pp. 15-18
Author(s):  
Piotr Szkudlarz

Abstract Hypericum elegans is a rare perennial distributed primarily in Central and Eastern Europe. Seed morphology and anatomy in H. elegans was studied on the basis of a seed sample from its only locality in Poland. Scanning electron microscopy revealed that the seed coat of mature seeds is composed basically of 3 cell layers: epidermal, subepidermal and sclerenchymatic. They are documented graphically here.


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