Seed morphology of perennial taxa of Euphorbia section Pithyusa (Euphorbiaceae) in Turkey

Phytotaxa ◽  
2018 ◽  
Vol 336 (3) ◽  
pp. 263
Author(s):  
İLKER GENÇ ◽  
ŞÜKRAN KÜLTÜR
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Seed Morphology ◽  
Seed Shape ◽  
Seed Surface ◽  
Surface Ornamentation ◽  
Seed Characteristics ◽  
Scanning Electron ◽  
Comprehensive Study

A comprehensive study based on seed morphology of perennial Euphorbia (Euphorbiaceae) sect. Pithyusa species occurring in Turkey is presented. A total of 14 species were studied. Seed characteristics were examined using scanning electron microscopy (SEM) as well as dissecting light microscopy. Significant features are: seed size, seed shape, shape of caruncle and seed surface ornamentation. Three different seed surface types (smooth, pitted, and wrinkled) were observed. Four main seed shapes (ovoid, oblong, quadrangular, and globose), as well as seven types of seed coat ornamentation (reticulate-areolate, areolate, alveolate, falsifoveate, pusticulate, colliculate and smooth) were found. The number of testa cells per 100 µm2 and also its range, from 8–12 to 57–63, are given.

scholarly journals Variation in seed morphology in the genus Erica L. (Ericaceae)

2009 ◽  
Vol 16 (1) ◽  
pp. 1-106 ◽  
Author(s):  
Piotr Szkudlarz
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cape Floristic Region ◽  
Seed Morphology ◽  
Key To Species ◽  
Tropical Africa ◽  
Seed Shape ◽  
Diagnostic Features ◽  
Seed Characteristics ◽  
Scanning Electron

Variation in seed morphology in the genusEricaL. (Ericaceae)Seed morphology was studied in 136 species ofErica, including 123 from South Africa (Cape Floristic Region), 5 from tropical Africa, and 8 from Europe. Seed anatomy was studied in 13 selected species by light and scanning electron microscopy. Seed morphology of each species was described and documented by SEM micrographs. Ten major diagnostic features were selected, concerning seed shape, primary sculpture, and fine relief. On the basis of these features, the species were divided into 14 groups. Within them, 40 morphologically homogeneous subgroups were distinguished, which could possibly be used in future revisions of the genus. A key to species identification has been developed on the basis of seed characteristics. A very close similarity was observed between seeds of someEricaspecies from distant parts of the distribution range of this genus.

Seed morphology of Epilobium and Chamaenerion (Onagraceae) in Turkey

Phytotaxa ◽  
2017 ◽  
Vol 331 (2) ◽  
pp. 169 ◽  
Author(s):  
KAMİL COŞKUNÇELEBİ ◽  
SERDAR MAKBUL ◽  
SEDA OKUR
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Morphology ◽  
Morphological Features ◽  
Seed Shape ◽  
Generic Level ◽  
Specific Level ◽  
Periclinal Wall ◽  
First Time ◽  
Scanning Electron

Macro- and micro-morphological features of seeds belonging to 26 taxa from Turkey were observed under the light and scanning electron microscopy. Present findings partly agree with segregation of Epilobium and Chamerion at generic level. The members of the genus Epilobium were distinguished by seeds with conical, semispherical, cylindrical or crest-like papillae or without papillae, granulate periclinal surfaces, papillae with parallel, radial, irregular or spirally furrow and the members of Chamerion were distinguished by seeds crest-like papillae or without papillae and without granulate periclinal surfaces and without furrow. The results also showed that seed shape, presence/absence of papillae and beak, papillae shape and ornamentation, and periclinal wall features are valuable for delimiting the examined taxa specific level within both genera. A key to Turkish Epilobium and Chamerion taxa based on seed morphology is presented for the first time

Portulaca oleracea aggregate (Portulacaceae) from the Chernivtsi Region (Ukraine)

2020 ◽  
Vol 12 (2) ◽  
pp. 251-262
Author(s):  
Olena Bulakh ◽  
Olena Volutsa ◽  
Alla Tokaryuk ◽  
Vasyl Budzhak ◽  
Ksenia Korzhan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Smooth Surface ◽  
Seed Morphology ◽  
Portulaca Oleracea ◽  
Scanning Microscope ◽  
Surface Ultrastructure ◽  
Seed Surface ◽  
Scanning Electron ◽  
Brown Color

At the results of investigation of micromorphology features of seed surface of the Portulaca oleracea aggr. (Portulacaceae Juss.) from Chernivtsi Region (Ukraine) six morphotypes of this taxa were recorded. Some peculiarities of the seed morphology of Portulaca L., and features of the ultrastructure of seed surface of the P. oleracea aggr. morphotypes, based on the results of micromorphological studies with using electronic scanning microscope was described. The general for the representative of the genus (e.g. globous, ovate or almost reniform shaped of seeds; black or dark brown color) and specify for the morphological types (star-shaped, isodiametric or elongate cells of seed surface with central part and long or short rays; smooth surface of seed or with knobs (from 0,03 mm in diameter) or papillae (0,01–0,02 mm), etc.) peculiarities are determined. The original scanning electron microscopy (SEM) photographs of seed surface ultrastructure of the studied morphological types and key fragment for its determination are suggested. According to our investigation six morphotypes of Portulaca oleracea aggr. are presented in Chernivtsi Region. Some of them are new for studied territory (P. granulatostellulata (Poelln.) Ricceri & Arrigoni, P. papillatostellulata (Danin & H.G. Baker) Danin), and other (Portulaca nitida (Danin & H.G. Baker) Ricceri & Arrigoni, P. trituberculata Danin, Domina & Raimondo, P. tuberculata (Danin & H.G. Baker) Danin) – new floristic records for Ukraine, and one of them (P. oleracea) – was known before also. The maps of distribution of this morphotypes in the region are presented. At the results of morphotypes distribution the most common is P. granulatostellulata and P. oleracea – rare in studied region like as in Europe also.

scholarly journals SEED MORPHOLOGY IN OnobrychisMILLERSECTION HYMENOBRYCHIS DC. FROM TURKEY

2015 ◽  
Vol 33 (4) ◽  
pp. 699-705 ◽  
Author(s):  
M. ÖZKAN ◽  
E. AKTOKLU ◽  
C. ÖZDEMIR
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Size ◽  
Seed Morphology ◽  
Identification Process ◽  
Diagnostic Characters ◽  
Seed Surface ◽  
Surface Micromorphology ◽  
Scanning Electron

ABSTRACT The seed (nutlet) morphology of four Onobrychis Miller (Fabaceae: subfamily Papilionoideae, Section Hymenobrychis DC.) taxa from Turkey, including three endemic taxa, was examined using scanning electron microscopy. Onobrychis tournefortii, O. galegifolia, O. cappadocica, O. albiflora. The seed examined exhibited variation in size, shape, colour, and surface sculpturing. Seed size ranged between 4.0-5.2 mm length and 2.0-3.6 mm width. Observed shapes included; ellipticus anguste asymmtricus reniformis, Ovatus anguste asymmetricus reniformis and ellipticus reniformis. Seed surface sculpturing revealed two distinct types: reticulate and rugulate. Species of Onobrychisare generally similar and confused with those of Hedysarumduring the identification process. Seed surface micromorphology can suggest taxonomical diagnostic characters for distinguishing species. Many of these characteristics are diagnostic at both the generic and specific levels.

scholarly journals Taxonomic Significance of Seed Morphology in Veronica L. (Plantaginaceae) Species from Central Europe

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 88
Author(s):  
Małgorzata Mazur ◽  
Katarzyna Marcysiak ◽  
Agnieszka Dunajska ◽  
Magdalena Gawlak ◽  
Tomasz Kałuski
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Morphology ◽  
Seed Shape ◽  
Periclinal Walls ◽  
Species Specific ◽  
Post Hoc ◽  
Microscopy Images ◽  
Scanning Electron

In this study, 1159 seeds of 29 Central European species of the genus Veronica were analyzed based on scanning electron microscopy images. The species belonged to nine subgenera: Beccabunga, Chamaedrys, Cochlidiosperma, Pellidosperma, Pentasepalae, Pocilla, Pseudolysimachium, Stenocarpon and Veronica, following the newest phylogenetic classification of the genus. Nine measured characteristics of seeds and nine ratios were analyzed statistically using ANOVA followed by post hoc testing, cluster analysis and discriminant analysis. In most cases, the results were not congruent with the contemporary classification of the genus. Examinations of qualitative seed features by scanning electron microscopy included the cochlidiospermous or discoid seed type, the seed shape, the general sculpture of the seed coat surface, the sculpture of anticlinal and periclinal walls and some species-specific traits such as the presence of the epidermal appendix. All these features, apart from seed shape, were useful to distinguish all subgenera and some species within subgenera: Beccabunga, Chamaedrys, Pellidosperma, Pocilla (only V. filiformis) and Veronica. The identification key based on the seed micromorphological features was prepared.

Taxonomic significance of capsule and seed characters of Indian species of Murdannia Royle (Commelinaceae)

Phytotaxa ◽  
2014 ◽  
Vol 178 (1) ◽  
pp. 1 ◽  
Author(s):  
Ancy Attullil Antony ◽  
Santhosh Nampy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Seed Morphology ◽  
Indian Species ◽  
Taxonomic Significance ◽  
Seed Characters ◽  
Scanning Electron

Capsule and seed morphology of 26 species of Murdannia in India are studied using light microscopy and seeds of 23 species by scanning electron microscopy. The capsules show differences in size, shape and in the number and arrangement of seeds in the locules. The seeds also display considerable variation in size, shape, ornamentation, shape of hilum and position of embryotega. The present study provides useful characters for the delimitation of Indian species of Murdannia.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Examination of Schistosome Cercariae and Schistosomules by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 50-51
Author(s):  
D. Johnson ◽  
P. Moriearty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Surface Structure ◽  
Extensive Study ◽  
Scanning Microscopy ◽  
Host Parasite ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.

SEM Observations on the Germination of Euonymous Americanus

1985 ◽  
Vol 43 ◽  
pp. 508-509
Author(s):  
D.R. Hill ◽  
J.R. McCurry ◽  
L.P. Elliott ◽  
G. Howard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Filter Paper ◽  
Room Temperature ◽  
Food Source ◽  
Environmental Chamber ◽  
Dormant Stage ◽  
Scanning Electron

Germination of Euonymous americanus in the laboratory has previously been unsuccessful. Ability to germinate Euonymous americanus. commonly known as the american strawberry bush, is important in that it represents a valuable food source for the white-tailed deer. Utilizing the knowledge that its seeds spend a period of time in the rumin fluid of deer during their dormant stage, we were successful in initiating germination. After a three month drying period, the seeds were placed in 25 ml of buffered rumin fluid, pH 8 at 40°C for 48 hrs anaerobically. They were then allowed to dry at room temperature for 24 hrs, placed on moistened filter paper and enclosed within an environmental chamber. Approximately four weeks later germination was detected and verified by scanning electron microscopy; light microscopy provided inadequate resolution. An important point to note in this procedure is that scarification, which was thought to be vital for germination, proved to be unnecessary for successful germination to occur. It is believed that germination was propagated by the secretion of enzymes or prescence of acids produced by microorganisms found in the rumin fluid since sterilized rumin failed to bring about germination.

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