Ultrastructural observations on the surface of Thelazia lacrymalis (Nematoda: Spirurida, Thelaziidae)

2005 ◽  
Vol 53 (2) ◽  
pp. 205-212 ◽  
Author(s):  
Soraya Naem
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Buccal Capsule ◽  
Ventral Side ◽  
Infected Cattle ◽  
Surface Ultrastructure ◽  
Small Papilla ◽  
Scanning Electron

Scanning electron microscopy (SEM) was used to study the surface ultrastructure of female Thelazia lacrymalis obtained from naturally infected cattle. At the anterior end, the buccal capsule was cup shaped. The mouth was without lips and was surrounded by four cephalic papillae and two amphids. There were two lateral cervical papillae. Also, a single small papilla was seen on the ventral side, close to the vulvar opening. The vulva was located posterior to the end of the oesophagus and the tail carried an anal pore and a pair of phasmids near the tip. The cuticle was transversally annulated with fine striations.

scholarly journals The species of Grammostola (Araneae: Theraphosidae) from Central Argentina: taxonomy, distribution, and surface ultrastructure of coxal setae

Zootaxa ◽  
2011 ◽  
Vol 2828 (1) ◽  
pp. 1 ◽  
Author(s):  
N. FERRETTI ◽  
G. POMPOZZI ◽  
F. PÉREZ-MILES
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Central Portion ◽  
Surface Ultrastructure ◽  
Central Argentina ◽  
Difficult Genus ◽  
Different Types ◽  
Basal Portion ◽  
New Synonymy ◽  
Scanning Electron

Three species of Grammostola (Araneae, Mygalomorphae, Theraphosidae) from central Argentina are redescribed and illustrated and their geographic distributions are reported. A new synonymy between G. vachoni Schiapelli & Gerschman 1961 and G. schulzei (Schmidt 1994) n. syn. is proposed. We did not find zones of co-occurrence with the three species but, in several localities, we found two Grammostola species in sympatry. The surface ultrastructure of three types of coxal setae was studied by scanning electron microscopy (SEM). Two morphological types of spiniform setae were found on the coxae. The first type consisted of short and strong piriform setae, with the surface completely covered by ridges, and the second type consisted of long and thinner spiniform setae, with a piriform basal portion. The basal portion of the setae had ridges, the central portion was smooth and the apical zone had numerous short barbs with curved tips. The different types of setae found in these Grammostola species are clearly distinguished, and could be new useful character to diagnose at least these species. In combination with other characteristics, these characters could probably help to solve the problems in other species of this difficult genus if not also other genera.

Oricymba tianmuensis sp. nov., a new cymbelloid species (Bacillariophyceae) from Tianmu Mountain in Zhejiang Province, China

Phytotaxa ◽  
2015 ◽  
Vol 236 (3) ◽  
pp. 257 ◽  
Author(s):  
Wei Zhang ◽  
Yan Ling Li ◽  
J Patrick Kociolek ◽  
Rui Lei Zhang ◽  
Li Qing Wang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Central Area ◽  
Ventral Side ◽  
Zhejiang Province ◽  
Similar Species ◽  
The West ◽  
West Region ◽  
Freshwater Diatoms ◽  
Scanning Electron

During a survey of freshwater diatoms from streams in the west region of the Tianmu Mountains, Zhejiang Province, China, a new Oricymba species, Oricymba tianmuensis sp. nov. has been recorded. A detailed morphology description of O. tianmuensis is presented, based on light and scanning electron microscopy. Oricymba tianmuensis sp. nov. is characterized by a ridge along the valve face typical of the genus, as well as a linear-lanceolate, symmetrical or slightly dorsiventral outline with a gibbous central portion gradually tapering or nearly paralleling towards the bluntly cuneate poles, a distinct rhombic-lanceolate asymmetric central area with a distinct stigma on the ventral side of the central nodule, a slightly lateral raphe with widened proximal endings and strongly deflected distal endings, and weakly radiate striae. Morphological features of the taxon are compared with similar species, and the biogeography of the genus is summarized.

scholarly journals Rhabdias bufonis (Rhabdiasidae) from the lung of the African common toad, Amietophrynus regularis (Bufonidae) in Egypt. New data on the basis of light and scanning electron microscopic study

2018 ◽  
Author(s):  
Kareem Morsy ◽  
Sara Ali ◽  
Fathy Abdel Ghaffar ◽  
Hoda El Fayoumi ◽  
Heba Abdel Haleem
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Habitat ◽  
Mouth Opening ◽  
Tail Length ◽  
Buccal Capsule ◽  
Common Toad ◽  
Electron Microscopic ◽  
Oral Opening ◽  
Scanning Electron

Background and Aims: Rhabdias bufonis (Rhabdiasidae) is a nematode parasite of family Rhabdiasidae infecting the lung of amphibians. The present study provides new morphological details for R. bufonis isolated from the lungs of the African common toad, Amietophrynus regularis based on observations of light and scanning electron microscopy. Methods: Forty specimens were collected during the period from March to August 2017 from its natural habitat; the damp, moist fields and gardens at Giza governorate, Egypt. Nematode worms were isolated from the lungs, fixed and then preserved. They were examined using light and scanning electron microscopy with further line drawings. Results: 14 specimens (35%) were found to harbor a large number of Rhabdias. Body of the gravid females were elongated, slender, measured 3.22–9.86 (5.64±0.03) long and 0.09–0.48 (0.23±0.02) mm wide at mid–length. Buccal capsule was with cylindrical lumen and sclerotised walls 13–31 (18 ± 2) long and 16–27 (24 ± 2) mm wide. Ovaries were almost straight. The uteri were located anterior and posterior to the vulva. Uterus filled with large number of eggs (17-42). Eggs were oval in shape, 0.120–0.132 (0.126±0.002) × 0.039–0.812 (0.517±0.02) mm and some of them were with fully developed larvae inside. The tail was comparatively short, gradually tapered, tail length 0.131–0.435 (0.320±0.02) mm long. SEM showed that worms possessed an oval anterior end with a simple, slit like oral opening. The lipless edges of mouth opening bordered with tiny cuticular elevations, radiated outwards. Two pairs of submedian cephalic papillae were observed around the oral opening as well as two amphids. Conclusion: The current study introduced new morphological details for R. bufonis isolated from the Egyptian frog, which was not reported in the previous studies, of them, the morphology of the slit–like mouth opening, the two pairs of cephalic papillae, two amphids and the three pairs of cuticular elevations supporting the area around mouth opening.

Morphology of the nematode Deletrocephalus dimidiatus Diesing, 1851 from the rhea, Rhea americana Linnaeus, 1758, together with a key to species of Deletrocephalinae

2016 ◽  
Vol 91 (2) ◽  
pp. 244-254 ◽  
Author(s):  
N.B. Ederli ◽  
F.C.R. Oliveira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Gastrointestinal Tract ◽  
Buccal Capsule ◽  
The Other ◽  
Key To Species ◽  
Morphometric Characters ◽  
Rhea Americana ◽  
The Family ◽  
Scanning Electron

AbstractThe family Deletrocephalidae consists of three species, from two genera: Deletrocephalus dimidiatus, D. cesarpintoi and Paradeletrocephalus minor, which differ from one another in terms of the buccal capsule structure, the dorsal ray from the male copulatory bursa and the length of the vagina in the female. All these species are parasites of the gastrointestinal tract of the rhea, Rhea americana. Only D. dimidiatus was reported to be a parasite of the other rhea species, Darwin's rhea, R. pennata. Currently, there are no studies on the ultrastructure and biology of these parasites or their pathogenicity in these birds. Thus, the present study aimed to examine the ultrastructure and add more details of the morphology of D. dimidiatus from R. americana. Four adult rheas were necropsied, and the gastrointestinal tract was collected and examined for the presence of parasites. Nematodes were analysed by optical and scanning electron microscopy (SEM). All birds were parasitized by D. dimidiatus. Some morphological and morphometric characters were observed that differ from reports of other studies of this species by other authors. In addition, new details were added, well described by SEM, and a key to the species of the subfamily Deletrocephalinae is given.

Sample Preparation to Observe The Straight And Flat Posture of Silkworm Embryo under Scanning Electron Microscopy via Glycerol Substitution Method

2014 ◽  
Vol 20 (3) ◽  
pp. 964-967
Author(s):  
Wenbin Liu ◽  
Chenzhao Li ◽  
Qi Zhang ◽  
Zhixin Lei ◽  
Yanqi Hou ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ventral Side ◽  
High Viscosity ◽  
Preparation Technique ◽  
Preparation Process ◽  
Substitution Method ◽  
Simple Method ◽  
Sem Observation ◽  
Scanning Electron

AbstractIn the preparation process for scanning electron microscopy (SEM), flexed silkworm embryos typically assume several curled shapes with irregular postures that obscure morphological details during SEM observation. We describe a preparation technique based on glycerol substitution for better SEM visualization of straight and flat silkworm embryos. Glycerol has high viscosity, low vapor pressure, and sufficient electrical conductivity. Silkworm embryos were infiltrated with glycerol and arranged in a straight posture or flattened using a cover slip. Samples were directly observed by SEM without additional dehydration, drying, or coating procedures. The complete ventral side could be easily viewed in one image. Recoating alleviated the charging phenomenon. This represents a simple method for preparation of straight and flat samples from curled biological specimens for SEM observation.

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