scholarly journals ISOLASI DAN UJI ANTIOKSIDAN HISPERIDIN DARI KULIT JERUK BALI (Citrus maxima Merr) SEBAGAI PENINGKAT IMUN UNTUK MENCEGAH COVID-19 DENGAN METODE DPPH.

2021 ◽  
Vol 8 (2) ◽  
pp. 53-61
Author(s):  
Tatiana Siska Wardani ◽  
Utomo Kartika Endrat ◽  
Mahadewi Putri Kezia ◽  
Al Fajri Razid Faizur Muhammad
Keyword(s):  
T Test ◽  
Protein Target ◽  
Citrus Maxima ◽  
Ft Ir

Kulit buah jeruk selama ini tidak dimanfaatkan  padahal kandungan senyawa kulit jeruk yang sangatlah sangatlah bervariasi salah satu senyawa yang terdapat dalam kulit jeruk jeruk adalah hesperidin yang termasuk golongan flavanoid. Senyawa hisperidin mempunyai potensi yang lebih tinggi dibanding senyawa pada lengkuas, secang, maupun kunyit.  Hal itu dikarenakan senyawa jeruk itu dapat mengikat kuat dengan mudah pada protein target pada virus maupun sel inang sehigga dapat ditarik kesimpulan senyawa di jeruk membuat blokade yang menghambat virus dalam berkembang melakukan replikasi  dan menginfeksi sel inang Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi hisperidin dari kulit jeruk dan mengetahui nilai IC50 . Ekstraksi dilakukan dengan metode soxletasi kemudian dilakukan identifikasi hisperidin kemudian dilanjutkan uji kemurnian isolat dengan penentuan titik leleh dan dilakukan identifikasi menggunakan spektrofotometri UV dan spektrofotometri infra merahdan FT-IR. Hasil yang diperoleh dari KLT, nilai Rf dari kulit buah jeruk manis jeruk 0,83 sedangkan nilai Rf pembanding hesperidin 0,80. Hasil spektrum UV dan IR menunjukkan bahwa isolat identik dengan hesperidin baku, sedangkan untuk uji antioksidan dilakukan dengan metode DPPH 0,1 mM dan hitung nilai IC50. Analisis statistik digunakan uji T-Test. Hasil uji DPPH ekstrak kulit jeruk bali memiliki aktivitas antioksidan kuat dengan nilai IC50 sebesar 39,77 µg/ml.  

scholarly journals Microwave-assisted extraction and characterization of pectin from citrus fruit wastes for commercial application

Food Research ◽  
2021 ◽  
Vol 5 (5) ◽  
pp. 80-88
Author(s):  
M.M. Mahmud ◽  
M. Belal ◽  
S. Ahmed ◽  
M.M. Hoque ◽  
W. Zzaman
Keyword(s):  
Citrus Fruit ◽  
Commercial Application ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Cosmetic Industry ◽  
Citrus Maxima ◽  
Ft Ir ◽  
Kinnow Mandarin

Pectin is an essential hydrocolloid widely used as a gelling, thickening, and stabilizing agent in the food, pharmaceutical, and cosmetic industry. In the present study, an effort has been made to extract pectin from Pomelo (Citrus maxima), Kinnow mandarin (Citrus reticulata) and Citron (Citrus medica) peels with microwave-assisted extraction (MAE) technique using organic citric acid. Pectin extracted with MAE was characterized in terms of yield, ash content, pH, solubility, equivalent weight, methoxyl content, anhydrouronic acid content, and degree of esterification. The extraction conditions had significant effects on physicochemical properties. The results showed that the highest amount of pectin (24.19±0.26%) was obtained from Citrus maxima. Based on DE value all pectins were categorized as low methoxy pectin (LMF). Extracted pectins were highly pure based on AUA content. Structural characterization of extracted pectin from three fruit peels by Fourier Transform Infrared (FT-IR) spectroscopy revealed that pectin has functional groups within the 1740–800 cm-1 spectral region. However, pectin extracted in this study can be used in the manufacturing of low sugar foods such as diet jams and jellies.

FT-IR microspectroscopic analysis of diseased white matter brain tissues

1995 ◽  
Vol 53 ◽  
pp. 968-969
Author(s):  
Steven M. Le Vine ◽  
David L. Wetzel
Keyword(s):  
White Matter ◽  
Gray Matter ◽  
Twitcher Mouse ◽  
Grid Pattern ◽  
Marked Contrast ◽  
Spatially Resolved ◽  
Ft Ir ◽  
Ir Microspectroscopy ◽  
Chemical Evidence

In situ FT-IR microspectroscopy has allowed spatially resolved interrogation of different parts of brain tissue. In previous work the spectrrscopic features of normal barin tissue were characterized. The white matter, gray matter and basal ganglia were mapped from appropriate peak area measurements from spectra obtained in a grid pattern. Bands prevalent in white matter were mostly associated with the lipid. These included 2927 and 1469 cm-1 due to CH2 as well as carbonyl at 1740 cm-1. Also 1235 and 1085 cm-1 due to phospholipid and galactocerebroside, respectively (Figs 1and2). Localized chemical changes in the white matter as a result of white matter diseases have been studied. This involved the documentation of localized chemical evidence of demyelination in shiverer mice in which the spectra of white matter lacked the marked contrast between it and gray matter exhibited in the white matter of normal mice (Fig. 3).The twitcher mouse, a model of Krabbe’s desease, was also studied. The purpose in this case was to look for a localized build-up of psychosine in the white matter caused by deficiencies in the enzyme responsible for its breakdown under normal conditions.

Ultra-spatially resolved synchrotron powered FT-IR microspectroscopy of individual cells of biological material

1995 ◽  
Vol 53 ◽  
pp. 884-885
Author(s):  
David L. Wetzel ◽  
John A. Reffner ◽  
Gwyn P. Williams
Keyword(s):  
Thermal Noise ◽  
Spot Size ◽  
Acquisition Time ◽  
Thermal Source ◽  
Signal To Noise ◽  
Spatially Resolved ◽  
Good Spatial Resolution ◽  
Small Spot ◽  
Ft Ir ◽  
Ir Microspectroscopy

Synchrotron radiation is 100 to 1000 times brighter than a thermal source such as a globar. It is not accompanied with thermal noise and it is highly directional and nondivergent. For these reasons, it is well suited for ultra-spatially resolved FT-IR microspectroscopy. In efforts to attain good spatial resolution in FT-IR microspectroscopy with a thermal source, a considerable fraction of the infrared beam focused onto the specimen is lost when projected remote apertures are used to achieve a small spot size. This is the case because of divergence in the beam from that source. Also the brightness is limited and it is necessary to compromise on the signal-to-noise or to expect a long acquisition time from coadding many scans. A synchrotron powered FT-IR Microspectrometer does not suffer from this effect. Since most of the unaperatured beam’s energy makes it through even a 12 × 12 μm aperture, that is a starting place for aperture dimension reduction.

FR-IR Molecular Microanalysis System

1990 ◽  
Vol 48 (2) ◽  
pp. 270-271
Author(s):  
John A. Reffner ◽  
William T. Wihlborg
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Integrated System ◽  
Morphological Examination ◽  
Fully Integrated ◽  
Ir Microscopy ◽  
Normal Functions ◽  
Infrared Microspectroscopy ◽  
Infrared Spectral ◽  
Ft Ir

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

Polarized microbeam FT-IR analysis of single fibers

1996 ◽  
Vol 54 ◽  
pp. 206-207
Author(s):  
Liling Cho ◽  
David L. Wetzel
Keyword(s):  
Molecular Orientation ◽  
Transition Point ◽  
Polymer Fibers ◽  
Single Fiber ◽  
Wire Grid ◽  
Polyester Fibers ◽  
Ft Ir ◽  
Ir Analysis ◽  
The Relationship ◽  
Wire Grid Polarizer

Polarized infrared microscopy has been used for forensic purposes to differentiate among polymer fibers. Dichroism can be used to compare and discriminate between different polyester fibers, including those composed of polyethylene terephthalate that are frequently encountered during criminal casework. In the fiber manufacturering process, fibers are drawn to develop molecular orientation and crystallinity. Macromolecular chains are oriented with respect to the long axis of the fiber. It is desirable to determine the relationship between the molecular orientation and stretching properties. This is particularly useful on a single fiber basis. Polarized spectroscopic differences observed from a single fiber are proposed to reveal the extent of molecular orientation within that single fiber. In the work presented, we compared the dichroic ratio between unstretched and stretched polyester fibers, and the transition point between the two forms of the same fiber. These techniques were applied to different polyester fibers. A fiber stretching device was fabricated for use on the instrument (IRμs, Spectra-Tech) stage. Tension was applied with a micrometer screw until a “neck” was produced in the stretched fiber. Spectra were obtained from an area of 24×48 μm. A wire-grid polarizer was used between the source and the sample.

Ergebnisse musiktherapeutischer Entspannung im sozialtherapeutischen Strafvollzug

2004 ◽  
Vol 15 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Andreas Zeuch ◽  
Thomas Hillecke
Keyword(s):  
T Test

Zusammenfassung. In diesem Artikel werden die qualitativ-quantitativen Ergebnisse einer Orientierungsstudie (2000-2002) zur Wirkung und Wirkungsweise musiktherapeutischer Entspannung im (sozialtherapeutischen) Strafvollzug vorgestellt. Die Untersuchungsgruppe umfasste n = 11 Teilnehmer, die jeweils über einen Zeitraum von einem halben Jahr mit Interviews zu drei Messzeitpunkten befragt wurden. Darüber hinaus kam in jeder Behandlungsstunde ein Prä-Post-Fragebogen zur Anwendung, der statistisch mit einem t-Test für abhängige Daten analysiert wurde. Als Ergebnis ergab sich ein Modell zur besonderen Wirkungsweise und Bedeutung von Musik als Entspannungsmedium im Strafvollzug sowie ein erster Signifikanznachweis der Wirkung der musiktherapeutischen Entspannung.

NO T-TEST FETISH

1975 ◽  
Vol 20 (9) ◽  
pp. 758-758
Author(s):  
Donald H. Kausler
Keyword(s):  
T Test
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