scholarly journals PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2017 ◽  
Vol 4 (1) ◽  
pp. 68
Author(s):  
M. H. Fariduddin Ath-thar ◽  
Intan Putriana ◽  
Dinar Tri Soelistyowati ◽  
Rudhy Gustiano
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Genetic Resources ◽  
Key Words ◽  
Genetic Relationship ◽  
Fragment Size ◽  
Random Amplified Polymorphic Dna ◽  
Tropical Species ◽  
Total Production ◽  
Culture Activity

Genotype performance of Tambakan, Helostoma temminckii (Cuvier, 1829) from Sumatera, Java and Kalimantan Polulation using Random Amplified Polymorphic DNA (RAPD)        Tambakan, kissing gourami (Helostoma temminckii) is well known as a freshwater tropical  species from Southeast Asia. In Indonesia, tambakan is an important commodity. However, total production of tambakan tends to decrease. Therefore, domestication has urgently to be done to solve the problem of Tambakan population. Three different tambakan populations from Sumatera, Java and Kalimantan were observed to find good genetic resources for culture activity. This study aimed to investigate the genetic variation of tambakan especially from West Java, Jambi, and South Kalimantan province using RAPD. The result showed that the highest polymorphism and heterozygosity was from South Kalimantan population among others. The three population observed had the fragment size ranged from 100- 2000 bp. The highest genetic distance was between Sumatera and Kalimantan (0,2877), while the lowest was between Kalimantan and Java (0,1961). Key words: Helostoma temminckii, genetic, heterozigosity, genetic relationship ABSTRAK        Ikan tambakan (Helostoma temminckii) adalah salah satu jenis ikan air tawar yang berasal dari wilayah tropis, tepatnya Asia Tenggara. Di Indonesia ikan tambakan merupakan salah satu ikan komoditas penting. Saat ini, jumlah produksi ikan tambakan cenderung menurun. Sehingga domestikasi sangat diperlukan untuk mengatasi masalah peneurunan populasi tambakan Tiga populasi ikan tambakan dari Sumatera, Jawa dan Kalimantan diobeservasi untuk mendapatkan sumber genetic terbaik yang dapat digunakan untuk kegiatan budidaya.. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetika populasi ikan tambakan Jambi, Jawa Barat dan Kalimantan Selatan menggunakan metode RAPD. Hasil menunjukkan bahwa polimorfisme dan heterosigositas tertinggi terdapat pada populasi ikan tambakan Kalimantan Selatan jika dibandingkan dengan populasi ikan tambakan lainnya. Ukuran fragmen DNA teramplifikasi berkisar antara 100-2000 bp. Jarak genetik paling jauh adalah antara populasi tambakan Sumatera dengan Kalimantan (0,2877), sedangkan jarak genetik terendah adalah tambakan Kalimantan dengan Jawa (0,1961).Kata kunci: Helostoma temminckii, genetik, heterosigositas, kekerabatan

scholarly journals KERAGAMAN GENETIK KELAPA DALAM BALI (DBI) DAN DALAM SAWARNA (DSA) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2020 ◽  
Vol 16 (2) ◽  
pp. 83
Author(s):  
DONATA S. PANDIN
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Key Words ◽  
Genetic Relationship ◽  
Banding Pattern ◽  
Numerical Taxonomy ◽  
Random Amplified Polymorphic Dna ◽  
Population Based ◽  
Plant Biology ◽  
Biology Laboratory

<p>ABSTRACT</p><p>Keragaman genetik dan hubungan kekerabatan dalam populasikelapa Dalam Bali (DBI) dan Dalam Sawarna (DSA) dianalisismenggunakan penanda RAPD. Penelitian dilaksanakan di LaboratoriumBiologi Tumbuhan, Pusat Penelitian Sumber Daya Hayati danBioteknologi, Institut Pertanian Bogor pada Februari-Mei 2007. Bahanyang digunakan dalam penelitian sebanyak 10 individu dari masing-masing populasi. Primer acak digunakan dalam analisis terdiri atas 10primer -10 mer yaitu OPA-02, OPA-08, OPA-10, OPA-13, OPA-20,OPB-08, OPB-11, OPB-12, OPB-15, OPB-20. DNA diekstraksimenggunakan metode Rohde yang telah dimodifikasi, konsentrasiditetapkan menggunakan metode Sambrook. Untuk melihat tingkatkekerabatan antar individu berdasarkan pola pita RAPD dari setiap primerdigunakan program NTsys ver. 2,0 (Program Numerical Taxonomy andMultivariate Analysis), sedangkan untuk analisis gerombol digunakanmetode UPGMA untuk membuat dendogram. Koefisien keragaman antarindividu dalam populasi kelapa DBI berkisar antara 2,4% – 30,7% denganrata-rata 21,7%, dan untuk populasi kelapa DSA antara 1,5% – 22,4%dengan rata-rata 12,7%. Jarak genetik individu-individu dalam populasikelapa Dalam Bali (DBI) cukup jauh menunjukkan bahwa keragamangenetik dalam populasi Dalam Bali masih tinggi, sehingga seleksi untukmaksud perbaikan sifat masih sangat memungkinkan. Pada populasikelapa Dalam Sawarna (DSA) jarak genetik individu-individu dalam sudahsemakin sempit, artinya keanekaragaman genetik antar individu di dalampopulasi DSA sudah sangat rendah oleh karena itu seleksi untuk maksudperbaikan sifat harus dilakukan dengan selektif. Hubungan kekerabatanantar populasi kelapa Dalam Bali dan Dalam Sawarna sebesar 44% artinyajarak genetik kedua populasi ini cukup jauh yaitu 56%. Sehingga jikaindividu-individu terseleksi dari kedua populasi tersebut disilangkan, akandiperoleh keturunan yang memilikinilai heterosis tinggi.</p><p>Kata kunci: Kelapa Dalam Bali, kelapa Dalam Sawarna, keragamangenetik, hubungan kekerabatan, RAPD</p><p>ABSTRACT</p><p>Genetic Diversity of Bali Tall (DBI) and Sawarna Tall(DSA) Coconuts Based  on  Random  AmplifiedPolymorphic DNA (RAPD</p><p>)Genetic Diversity of Bali Tall (DBI) and Sawarna Tall (DSA)coconuts based on Random Amplified Polymorphic DNA (RAPD) wasobserved. Ten plants were used in each population. The objectives of thisresearch were to determine genetic diversity within-and inter-population ofBali Tall (DBI) and Sawarna Tall (DSA) coconuts, and geneticrelationship of those population based on RAPD (Random AmplifiedPolymorphic DNA). Research was done in Plant Biology Laboratory ofCenter Research of Genetic Resources and Biotechnology, InstitutPertanian Bogor, February – May 2007. DNA extraction was done bymodified Rohde method and to determine the concentration and quality ofDNA by Sambrook method. Ten RAPD 10-mer were used namely OPA-02, OPA-08, OPA-10, OPA-13, OPA-20, OPB-08, OPB-11, OPB-12,OPB-15, OPB-20. To find out the level of genetic relationship betweenindividuals based on RAPD banding pattern of each primer, we usedNTsys program ver. 2.0 (program Numerical Taxonomy and MultivariateAnalysis System), whereas for the analysis of clustering UPGMA methodis used to create a dendogram. These ten RAPD primers could separateDBI and DSA in each group. Genetic diversity within-population of BaliTall coconut population varied from 2.4 to 30.7% with average of 21.7%.So that, opportunity to improve characters in DBI coconut populationcould be done by selection. Genetic diversity within-population ofSawarna Tall coconut population progressively was narrow, ranging from1.5 to 12.4% with average 12.7%, so the selection in order to do characterimprovement in this population could be done selectively. Geneticrelationship between DBI dan DSA populations was far enough (54%), sothe crossing between those population will be good for charactersimprovment.</p><p>Key words : Bali Tall coconut, Sawarna Tall coconut, genetic diversity,genetic relationship, RAPD</p>

Rapd variation of late-maturing sorghum [ Sorghum bicolor (L.) Moench] landraces from Ethiopia

2007 ◽  
Vol 55 (3) ◽  
pp. 375-382 ◽  
Author(s):  
S. Mamo ◽  
A. Ayana ◽  
T. Tesso
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Sorghum Bicolor ◽  
Rapd Markers ◽  
Diversity Index ◽  
Random Amplified Polymorphic Dna ◽  
The Mean ◽  
Polymorphic Bands ◽  
High Degree ◽  
Sorghum Bicolor L

A study on the extent and pattern of genetic variability in late-maturing sorghum [ Sorghum bicolor (L.) Moench] landraces collected from the Wello and Hararge areas of Ethiopia was conducted using random amplified polymorphic DNA (RAPD) markers for 70 individuals representing 14 populations. Four oligonucleotide primers generated a total of 55 polymorphic bands with 13–19 bands per primer and a mean of 16 bands across the 70 individuals. The value of the Shannon diversity index among the populations (0.26) and between the two regions (0.24) was low to moderate, despite the high degree of polymorphic bands per primer. The mean genetic distance (0.25) between the populations was found to be low. The low genetic variation may be due to the reduced population size of late-maturing sorghum landraces in the two regions of Ethiopia because of farmers’ decisions in the process of planting, managing, harvesting and processing their crops. Partitioning of the genetic variation into variation between and within the population revealed that 92.9% and 7.10% of the variation was found to be between and within the populations, respectively. Cluster analysis of genetic distance estimates further confirmed a low level of differentiation in late-maturing sorghum populations both between and within the regions. The implications of the results for genetic conservation purposes are discussed.

scholarly journals Hubungan Genetik antara Domba Wonosobo (Dombos), Domba Ekor Tipis (DET) dan Domba Batur (Dombat) Melalui Analisis Polimorfisme Protein Darah

2017 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
F Noviani ◽  
Sutopo Sutopo ◽  
E Kurnianto
Keyword(s):  
Thin Layer ◽  
Genetic Distance ◽  
Blood Plasma ◽  
Key Words ◽  
Genetic Relationship ◽  
Gel Electrophoresis ◽  
Gene Frequency ◽  
Genetic Relationships ◽  
Blood Protein ◽  
Polymorphism Analysis

<p>The objective of this study was to evaluate the genetic relationships among Wonosobo sheep (Dombos), Thin-Tailed sheep (DET) and Batur sheep (Dombat) through blood protein polymorphism analysis by using electrophoresis Polyacrylamid Gel Electrophoresis-Thin Layer Electrophoresis (PAGE-TLE). The blood of the 20 head of Dombos (Wonosobo regency), 20 head of DET (Semarang regency) and 20 head of Dombat (Banjarnegara regency) were used as materials. Six locus of blood plasma protein were observed, then the gene frequency, individual heterosigosity, average<br />heterosigosity and genetic distance among three breed were calculated. The results showed that the locus of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I), at DET, Dombos and Dombat were polymorphic (0,380-0,454). In conclusion, the DET and Dombat indicated the closer genetic relationship compared to DET-Dombos and Dombos-Dombat.<br />Key words: sheep, polymorphism, genetic relationship</p>

scholarly journals Genetic Diversity between Yacon Landraces from Different Countries Based on Random Amplified Polymorphic DNAs

2011 ◽  
Vol 41 (No. 2) ◽  
pp. 73-78 ◽  
Author(s):  
L. Milella ◽  
J. Salava ◽  
G. Martelli ◽  
I. Greco ◽  
E.F. Cusimamani ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Relationship ◽  
Genetic Similarity ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Coefficient ◽  
Crop Germplasm ◽  
Relationship Of

Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relationship of crop germplasm. Five different landraces of yacon (Smallantus sonchifolius (Poepp. and Hendl.) H. Robinson; Asteraceae) collected in various countries and showing different morphological traits were investigated using a total of 61 decamer primers. A total of 282 RAPD markers were scored and 28.7% of them were polymorphic at least within landraces. RAPD markers generated by one primer (OBP14) discriminated between all landraces. Markers were used to calculate genetic similarity coefficient and to build a dendrogram representing the genetic relationship between analysed landraces. The results suggest that RAPD markers could be used as a reliable tool to perform fingerprinting studies in Smallantus sonchifolius genome. This is the first report on the use of RAPDs to evaluate genetic distance and to distinguish between different landraces in yacon. &nbsp;

scholarly journals Molecular Characterization of 28 Mango Germplasm Using RAPD

1970 ◽  
Vol 17 (1) ◽  
pp. 71-77 ◽  
Author(s):  
ML Rahman ◽  
MG Rabbani ◽  
MNA Siddique ◽  
MA Rahman ◽  
EJ Garvey ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Rapd Analysis ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Index ◽  
The Other ◽  
Germplasm Characterization ◽  
Upgma Dendrogram

Genetic variation and relationship among 28 mango germplasm were analyzed using Random Amplified Polymorphic DNA (RAPD). Out of 20 primers screened, four were selected, which gave 50 clear and bright fragments, out of which 48 fragments were considered polymorphic. The proportion of polymorphic loci and gene diversity values across all loci were 96% and 0.29, respectively. The UPGMA dendrogram based on genetic distance segregated the 28 mango germplasm into two main clusters. Sukul alone formed one cluster and the rest germplasm were grouped together into another cluster. Mallika and Amrapali cultivar pair was very close to each other with the highest intervarietal similarity index (87.30%) and lowest genetic distance (0.08). On the other hand, Sukul and Meghnath pair was more distant to each other with the lowest intervarietal similarity index (14.29%) and highest genetic distance (0.87). The results of the present study indicated that the RAPD analysis could be utilized by breeders for further improvement of mango varieties.Key words: Germplasm, Characterization, Mango, RAPDDOI = 10.3329/ptcb.v17i1.1123Plant Tissue Cult. & Biotech. 17(1): 71-77, 2007 (June)

scholarly journals Assessment of Genetic Relationship Among Landraces of Bangladeshi Ridge Gourd (Luffa acutangula Roxb.) Using RAPD Markers

2009 ◽  
Vol 1 (3) ◽  
pp. 615-623 ◽  
Author(s):  
S. Hoque ◽  
M. G. Rabbani
Keyword(s):  
Genetic Distance ◽  
Genetic Relationship ◽  
Genetic Relatedness ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Index ◽  
Group Method ◽  
Pair Group ◽  
Average Gene ◽  
Luffa Acutangula

Information on genetic relatedness among ridge gourd (Luffa acutangula) genotypes from Bangladesh is currently not reported. Twenty eight accessions collected from different parts of Bangladesh were studied using random amplified polymorphic DNA (RAPD) technique. Four selected decamer primers, out of sixteen screened, could generate a total of 27 RAPD fragments of which 22 were polymorphic (81.5%). The bands ranged from 50 to 1500 bp in size. Genetic variation statistics for all loci estimated the average gene diversity (h) value as 0.278 and the Shannon’s Information Index (I) as 0.415. Dendrogram based on unweighted pair-group method with arithmetic averages (UPGMA) segregated the accessions into five clusters. Cluster III was the largest with 13 members followed by cluster II, V, I and IV with 6, 4, 3 and 2 members, respectively. Accession LA27 and LA29 were found very close to each other with the highest inter-variety similarity index (96.05%) and the lowest genetic distance (0.077); whereas accession LA40 and LA72 were more distant to each other with the lowest inter-variety similarity index (44.43%) and the highest genetic distance (0.73). A DNA extraction method has been standardized. The marker was found to be useful tool for assessing genetic variations in Luffa acutangula. Keywords: Genetic relationship; Germplasm; Ridge gourd; Luffa acutangula; RAPD.  © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i3.1968               J. Sci. Res. 1 (3), 615-623 (2009) 

scholarly journals HUBUNGAN KEKERABATAN GENETIK ANTAR SEMBILAN AKSESI KELAPA ASAL PROVINSI SULAWESI UTARA

2020 ◽  
Vol 15 (3) ◽  
pp. 139
Author(s):  
ELSJE TENDA ◽  
MEITY TULALO ◽  
MIFTAHORRACHMAN MIFTAHORRACHMAN
Keyword(s):  
Genetic Distance ◽  
Key Words ◽  
Genetic Relationship ◽  
Genetic Relationships ◽  
Cocos Nucifera ◽  
Fruit Weight ◽  
Breeding Material ◽  
North Sulawesi ◽  
Cocos Nucifera L ◽  
Germplasm Accessions

<p>ABSTRAK</p><p>Informasi jarak genetik dan hubungan kekerabatan sangatdiperlukan dalam merakit varietas unggul. Semakin jauh jarak genetikantar tetua maka peluang dihasilkan kultivar baru dengan variabilitasgenetik yang luas akan menjadi semakin besar. Sebaliknya, persilanganantar tetua berkerabat dekat akan menghasilkan variabilitas yang sempit.Penelitian ini dilaksanakan pada bulan Mei 2007 di Kabupaten MinahasaUtara, Minahasa Selatan dan Bolaang Mongondow, Sulawesi Utara. Disetiap kabupaten ditetapkan tiga desa contoh pada ketinggian yangberbeda. Tujuan penelitian ini adalah untuk mengetahui tingkatkekerabatan dan jarak genetik sembilan aksesi plasma nutfah kelapa asalSulawesi Utara yang akan digunakan sebagai materi pemuliaan dalamperakitan kelapa unggul. Untuk mengetahui kekerabatan antara sembilanaksesi kelapa tersebut diukur jarak genetiknya dengan menggunakanperhitungan nilai D 2 statistik dari Mahalanobis didasarkan pada delapankarakter komponen buah, yaitu panjang buah, lebar buah, berat buah utuh,berat buah tanpa sabut, berat buah tanpa air, berat daging buah, tebaldaging buah, dan berat tempurung. Hasil penelitian menunjukkan bahwakesembilan aksesi kelapa tersebut membentuk lima kelompok dan jarakgenetik terbesar terdapat antara kelompok II (Dalam Lansot, DalamMongkonai, Dalam Dua Saudara) dan IV (Dalam Kaleosan, Dalam Kema)dengan nilai D 2 = 2.196,57. Sumbangan terbesar terjadinya jarak genetiktersebut diperoleh dari karakter tebal daging buah.</p><p>Kata kunci : Cocos nucifera L., kekerabatan, genetik, kelapa dalam</p><p>ABSTRACT</p><p>Genetic relationship among nine coconut accessions fromNorth SulawesiThe research was conducted in May 2007 at North Minahasa, SouthMinahasa, and Bolaang Mongondow Regions, North Sulawesi Province.From each region, three villages with different elevation were determined.The objective of the research was to find out genetic relationship amongnine coconut germplasm accessions for breeding material in composinghigh yielding coconut. The genetic relationships were estimated using D 2Mahalanobis Statistics based on eight characters of fruit component, suchas length of fruit, width of fruit, fruit weight, unhusked fruit weight,weight of fruit without water, weight of endosperm, thickness of kernel,and weight of shell. The result showed that the nine accessions weredivided into five groups and the widest genetic distance had been foundbetween group II (Lansot Tall, Mongkonai Tall, Dua Saudara Tall) and IV(Kaleosan Tall, Kema Tall) with the D 2  value of 2,196.57. The highestcontribution to the genetic relationship was thick of kernel (50%contribution). Lansot Tall and Kaleosan Tall can be used as parents forprepotent coconut.</p><p>Key words : Cocos nucifera L., genetic relationship, tall coconut</p>

scholarly journals Hubungan Genetik antara Domba Wonosobo (Dombos), Domba Ekor Tipis (DET) dan Domba Batur (Dombat) Melalui Analisis Polimorfisme Protein Darah

2017 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
F Noviani ◽  
Sutopo Sutopo ◽  
E Kurnianto
Keyword(s):  
Thin Layer ◽  
Genetic Distance ◽  
Blood Plasma ◽  
Key Words ◽  
Genetic Relationship ◽  
Gel Electrophoresis ◽  
Gene Frequency ◽  
Genetic Relationships ◽  
Blood Protein ◽  
Polymorphism Analysis

<p>The objective of this study was to evaluate the genetic relationships among Wonosobo sheep (Dombos), Thin-Tailed sheep (DET) and Batur sheep (Dombat) through blood protein polymorphism analysis by using electrophoresis Polyacrylamid Gel Electrophoresis-Thin Layer Electrophoresis (PAGE-TLE). The blood of the 20 head of Dombos (Wonosobo regency), 20 head of DET (Semarang regency) and 20 head of Dombat (Banjarnegara regency) were used as materials. Six locus of blood plasma protein were observed, then the gene frequency, individual heterosigosity, average<br />heterosigosity and genetic distance among three breed were calculated. The results showed that the locus of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I), at DET, Dombos and Dombat were polymorphic (0,380-0,454). In conclusion, the DET and Dombat indicated the closer genetic relationship compared to DET-Dombos and Dombos-Dombat.<br />Key words: sheep, polymorphism, genetic relationship</p>

scholarly journals Genetic variation in relations in different goat populations of Bangladesh

1970 ◽  
Vol 39 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
MF Afroz ◽  
MO Faruque ◽  
SS Husain ◽  
JL Han ◽  
B Paul
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Key Words ◽  
Genetic Relation ◽  
Allele Number ◽  
Hilly Region ◽  
Different Populations

The genetic relation among different populations and breeds of goats in Bangladesh was studied using 15 microsatellite markers. A total of 181 goat samples- 36 from Black Bengal of western part (BBW), 39 from Black Bengal of central part (BBC), 40 from Black Bengal of Hilly region (BBH), 28 from exotic breeds (JAM) and 39 from crossbred (CRW) were genotyped. The allele number per locus ranged from 2 to 12. The average number of alleles per population was 5.69 ± 2.32, 5.38 ± 2.02, 5.23 ± 2.28, 6.08 ± 2.33, and 5.77 ± 2.35 for BBW, BBC, BBH, CRW and JAM, respectively. Heterozygosity was found in the range from 0.5049 ± 0.0222 (BBC) to 0.5751 ± 0.0262 (JAM). The genetic distance (DA) between BBC and JAM was the highest (0.0627) and that between JAM and CRW was the lowest (0.0037). In the phylogenetic dendrogram, BBC and BBH grouped in the same cluster, while CRW and JAM formed another cluster. BBW formed a third cluster between those two clusters. The closest genetic relation between BBW and CRW indicates the introgression of exotic genes occurred more in BBW than BBC and BBH. Key words: Bangladeshi goats; Microsatellite markers; Genetic variation; Dendrogram  DOI: http://dx.doi.org/10.3329/bjas.v39i1-2.9672 Bang. J. Anim. Sci. 2010, 39(1&2): 1-8

scholarly journals Genetic variation and molecular relationships among eight taxa of Desmodium Desv. based on RAPD markers

2017 ◽  
Vol 24 (2) ◽  
pp. 149-154
Author(s):  
M. Oliur Rahman ◽  
Md. Zahidur Rahman ◽  
Sonia Khan Sony ◽  
Mohammad Nurul Islam
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Genetic Relatedness ◽  
Random Amplified Polymorphic Dna ◽  
Banding Patterns ◽  
Upgma Dendrogram ◽  
Close Proximity ◽  
Plant Taxon ◽  
Molecular Relationships

Genetic variation and molecular relationships among eight taxa of Desmodium Desv. were assessed on the basis of random amplified polymorphic DNA (RAPD) markers. The banding patterns of eight taxa namely, Desmodium gangeticum (L.) DC., D. heterocarpon (L.) DC., D. heterophyllum (Willd.) DC., D. motorium (Houtt.) Merr., D. pulchellum (L.) Benth., D. triflorum (L.) DC., D. triquetrum (L.) DC. and D. triquetrumsubsp. alatum (DC.) Prain were compared. A total of 81 DNA fragments were detected by 11 primers. Among the taxa studied D. triquetrum and D. triquetrum subsp. alatum were found to be most closely related followed by close proximity between D. gangeticum and D. motorium. The highest genetic distance was observed between D. triflorum and D. heterophyllum followed by D. heterocarpon and D. heterophyllum. UPGMA dendrogram was constructed to show the genetic relatedness among the taxa employed and the tree revealed a close proximity among D. pulchellum, D. gangeticum and D. motorium. In contrast, D. heterophyllum was found distantly related with rest of the taxa.Bangladesh J. Plant Taxon. 24(2): 149–154.

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