Hubungan Genetik antara Domba Wonosobo (Dombos), Domba Ekor Tipis (DET) dan Domba Batur (Dombat) Melalui Analisis Polimorfisme Protein Darah

The objective of this study was to evaluate the genetic relationships among Wonosobo sheep (Dombos), Thin-Tailed sheep (DET) and Batur sheep (Dombat) through blood protein polymorphism analysis by using electrophoresis Polyacrylamid Gel Electrophoresis-Thin Layer Electrophoresis (PAGE-TLE). The blood of the 20 head of Dombos (Wonosobo regency), 20 head of DET (Semarang regency) and 20 head of Dombat (Banjarnegara regency) were used as materials. Six locus of blood plasma protein were observed, then the gene frequency, individual heterosigosity, average heterosigosity and genetic distance among three breed were calculated. The results showed that the locus of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I), at DET, Dombos and Dombat were polymorphic (0,380-0,454). In conclusion, the DET and Dombat indicated the closer genetic relationship compared to DET-Dombos and Dombos-Dombat. Key words: sheep, polymorphism, genetic relationship

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Hubungan Genetik antara Domba Wonosobo (Dombos), Domba Ekor Tipis (DET) dan Domba Batur (Dombat) Melalui Analisis Polimorfisme Protein Darah

Sains Peternakan ◽

10.20961/sainspet.v11i1.4798 ◽

2017 ◽

Vol 12 (1) ◽

pp. 1

Author(s):

F Noviani ◽

Sutopo Sutopo ◽

E Kurnianto

Keyword(s):

Thin Layer ◽

Genetic Distance ◽

Blood Plasma ◽

Key Words ◽

Genetic Relationship ◽

Gel Electrophoresis ◽

Gene Frequency ◽

Genetic Relationships ◽

Blood Protein ◽

Polymorphism Analysis

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HUBUNGAN KEKERABATAN GENETIK ANTAR SEMBILAN AKSESI KELAPA ASAL PROVINSI SULAWESI UTARA

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v15n3.2009.139-144 ◽

2020 ◽

Vol 15 (3) ◽

pp. 139

Author(s):

ELSJE TENDA ◽

MEITY TULALO ◽

MIFTAHORRACHMAN MIFTAHORRACHMAN

Keyword(s):

Genetic Distance ◽

Key Words ◽

Genetic Relationship ◽

Genetic Relationships ◽

Cocos Nucifera ◽

Fruit Weight ◽

Breeding Material ◽

North Sulawesi ◽

Cocos Nucifera L ◽

Germplasm Accessions

ABSTRAKInformasi jarak genetik dan hubungan kekerabatan sangatdiperlukan dalam merakit varietas unggul. Semakin jauh jarak genetikantar tetua maka peluang dihasilkan kultivar baru dengan variabilitasgenetik yang luas akan menjadi semakin besar. Sebaliknya, persilanganantar tetua berkerabat dekat akan menghasilkan variabilitas yang sempit.Penelitian ini dilaksanakan pada bulan Mei 2007 di Kabupaten MinahasaUtara, Minahasa Selatan dan Bolaang Mongondow, Sulawesi Utara. Disetiap kabupaten ditetapkan tiga desa contoh pada ketinggian yangberbeda. Tujuan penelitian ini adalah untuk mengetahui tingkatkekerabatan dan jarak genetik sembilan aksesi plasma nutfah kelapa asalSulawesi Utara yang akan digunakan sebagai materi pemuliaan dalamperakitan kelapa unggul. Untuk mengetahui kekerabatan antara sembilanaksesi kelapa tersebut diukur jarak genetiknya dengan menggunakanperhitungan nilai D 2 statistik dari Mahalanobis didasarkan pada delapankarakter komponen buah, yaitu panjang buah, lebar buah, berat buah utuh,berat buah tanpa sabut, berat buah tanpa air, berat daging buah, tebaldaging buah, dan berat tempurung. Hasil penelitian menunjukkan bahwakesembilan aksesi kelapa tersebut membentuk lima kelompok dan jarakgenetik terbesar terdapat antara kelompok II (Dalam Lansot, DalamMongkonai, Dalam Dua Saudara) dan IV (Dalam Kaleosan, Dalam Kema)dengan nilai D 2 = 2.196,57. Sumbangan terbesar terjadinya jarak genetiktersebut diperoleh dari karakter tebal daging buah.Kata kunci : Cocos nucifera L., kekerabatan, genetik, kelapa dalamABSTRACTGenetic relationship among nine coconut accessions fromNorth SulawesiThe research was conducted in May 2007 at North Minahasa, SouthMinahasa, and Bolaang Mongondow Regions, North Sulawesi Province.From each region, three villages with different elevation were determined.The objective of the research was to find out genetic relationship amongnine coconut germplasm accessions for breeding material in composinghigh yielding coconut. The genetic relationships were estimated using D 2Mahalanobis Statistics based on eight characters of fruit component, suchas length of fruit, width of fruit, fruit weight, unhusked fruit weight,weight of fruit without water, weight of endosperm, thickness of kernel,and weight of shell. The result showed that the nine accessions weredivided into five groups and the widest genetic distance had been foundbetween group II (Lansot Tall, Mongkonai Tall, Dua Saudara Tall) and IV(Kaleosan Tall, Kema Tall) with the D 2 value of 2,196.57. The highestcontribution to the genetic relationship was thick of kernel (50%contribution). Lansot Tall and Kaleosan Tall can be used as parents forprepotent coconut.Key words : Cocos nucifera L., genetic relationship, tall coconut

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PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

Jurnal Sains Natural ◽

10.31938/jsn.v4i1.77 ◽

2017 ◽

Vol 4 (1) ◽

pp. 68

Author(s):

M. H. Fariduddin Ath-thar ◽

Intan Putriana ◽

Dinar Tri Soelistyowati ◽

Rudhy Gustiano

Keyword(s):

Genetic Variation ◽

Genetic Distance ◽

Genetic Resources ◽

Key Words ◽

Genetic Relationship ◽

Fragment Size ◽

Random Amplified Polymorphic Dna ◽

Tropical Species ◽

Total Production ◽

Culture Activity

Genotype performance of Tambakan, Helostoma temminckii (Cuvier, 1829) from Sumatera, Java and Kalimantan Polulation using Random Amplified Polymorphic DNA (RAPD) Tambakan, kissing gourami (Helostoma temminckii) is well known as a freshwater tropical species from Southeast Asia. In Indonesia, tambakan is an important commodity. However, total production of tambakan tends to decrease. Therefore, domestication has urgently to be done to solve the problem of Tambakan population. Three different tambakan populations from Sumatera, Java and Kalimantan were observed to find good genetic resources for culture activity. This study aimed to investigate the genetic variation of tambakan especially from West Java, Jambi, and South Kalimantan province using RAPD. The result showed that the highest polymorphism and heterozygosity was from South Kalimantan population among others. The three population observed had the fragment size ranged from 100- 2000 bp. The highest genetic distance was between Sumatera and Kalimantan (0,2877), while the lowest was between Kalimantan and Java (0,1961). Key words: Helostoma temminckii, genetic, heterozigosity, genetic relationship ABSTRAK Ikan tambakan (Helostoma temminckii) adalah salah satu jenis ikan air tawar yang berasal dari wilayah tropis, tepatnya Asia Tenggara. Di Indonesia ikan tambakan merupakan salah satu ikan komoditas penting. Saat ini, jumlah produksi ikan tambakan cenderung menurun. Sehingga domestikasi sangat diperlukan untuk mengatasi masalah peneurunan populasi tambakan Tiga populasi ikan tambakan dari Sumatera, Jawa dan Kalimantan diobeservasi untuk mendapatkan sumber genetic terbaik yang dapat digunakan untuk kegiatan budidaya.. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetika populasi ikan tambakan Jambi, Jawa Barat dan Kalimantan Selatan menggunakan metode RAPD. Hasil menunjukkan bahwa polimorfisme dan heterosigositas tertinggi terdapat pada populasi ikan tambakan Kalimantan Selatan jika dibandingkan dengan populasi ikan tambakan lainnya. Ukuran fragmen DNA teramplifikasi berkisar antara 100-2000 bp. Jarak genetik paling jauh adalah antara populasi tambakan Sumatera dengan Kalimantan (0,2877), sedangkan jarak genetik terendah adalah tambakan Kalimantan dengan Jawa (0,1961).Kata kunci: Helostoma temminckii, genetik, heterosigositas, kekerabatan

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Genetic relationships in Saccharum complex germplasm collections based on morphological and molecular markers

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v89i1.421 ◽

2021 ◽

Vol 89 (1) ◽

Author(s):

Wiwit Budi WIDYASARI ◽

. DAMANHURI

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Genetic Distance ◽

Genetic Relationship ◽

Genetic Similarity ◽

Genetic Relationships ◽

Morphological Markers ◽

Saccharum Complex ◽

High Genetic Diversity ◽

Germplasm Collections

and is a cross-pollinator, so information about the genetic relationship between the accessions in germplasm collections is very important for selecting the prospective parent in crossbreeding. This research aims to determine the phylogenetic relationship of 24 Saccharum complex accessions and to verify the grouping of accessions using 37 morphological and three microsatellite molecular markers. Interpretation of morphological and molecular data was obtained from the analysis using the NTYSYpc-2.02i program. The results show that within the 24 accessions analyzed using morphological markers, some accessions did not cluster as the classification at the conservation time. This difference is due to the morphological markers, descriptions of characters that do not appear are considered as the same variables though in different morphological characters, so this increases the value of genetic similarity among accessions analyzed. In contrast, the grouping of molecular markers shows that each accession was grouped according to the classification at the conservation time. These accessions had a low genetic similarity of 0.20 with a broad genetic distance of 0.80. This broad genetic distance indicates that the twenty-four accessions have a distant genetic relationship with one another, so that the genetic diversity of these accessions is relatively high. The high genetic diversity in germplasm collections improves its potential as a crossing parent to obtain a high heterosis effect.

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Keragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah

Livestock and Animal Research ◽

10.20961/lar.v19i3.54921 ◽

2021 ◽

Vol 19 (3) ◽

pp. 247

Author(s):

Sutopo Sutopo ◽

Dela Ayu Lestari ◽

Asep Setiaji ◽

Edy Kurnianto

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Gene Frequency ◽

Blood Protein ◽

Muscovy Duck ◽

Central Java ◽

Cairina Moschata ◽

Electrophoresis Method ◽

Muscovy Ducks ◽

I Gene

Objective: The aim of this study was to identified genetic diversity of muscovy duck in Central Java through blood protein polymorphisms by using electrophoresis method.Methods: Blood sample was collected from a total of 60 muscovy ducks from the districts of Demak, Magelang and Pekalongan, 20 samples each. The Electrophoresis Polyacrylamide Gel of Electrophoresis–Thin Layer Electrophoresis (PAGE-TLE) was used in this study. Parameters observed were protein of albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and amylase-I (Am-I). Gene frequency, heterozygosity (Ho) and genetic distance were analyzed by using DISPAN program.Results: The results showed that the Alb, Cp, Tf and Am-I of muscovy duck showing polymorphic characters. Gene frequency of AlbB (0.61) was higher compared to AlbA (0.39). Gene frequency of CpA (0.62) was higher than the CpB (0.38). Furthermore, gene frequency of TfA and TfB were 0.32 and 0.68, respectively as well as gene frequency of Am-IA (0.82) was higher than Am-IB (0.18). Muscovy duck population of Demak showed higher Ho value for Alb and Am-I, whereas Magelang showed higher value for Cp and Tf. Population of muscovy duck of Demak showed closer genetic distance to Pekalongan than Magelang.Conclusions: The protein blood locus of three population of muscovy duck in Central Jawa showed polymorphic characters.

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Prediction of heterosis in wheat using coefficient of parentage and RFLP-based estimates of genetic relationship

Genome ◽

10.1139/g96-144 ◽

1996 ◽

Vol 39 (6) ◽

pp. 1142-1149 ◽

Cited By ~ 55

Author(s):

J. F. Barbosa-Neto ◽

M. E. Sorrells ◽

G. Cisar

Keyword(s):

Genetic Distance ◽

Genetic Relationship ◽

Genetic Relationships ◽

Triticum Aestivum L ◽

Hybrid Breeding ◽

Gene Pools ◽

Coefficient Of Parentage ◽

Hybrid Testing ◽

Key Words Wheat ◽

Reduced Time

Genetic relationship can be a useful predictor of the relative performance of hybrid combinations for a hybrid breeding program resulting in reduced time and cost of hybrid testing. Genetic relationships of 112 wheat (Triticum aestivum L. em. Thell.) lines were estimated using 41 DNA clones hybridizing to 273 DNA fragments and by calculating coefficient of parentage (COP). Heterosis was estimated for 722 hybrids grown in multiple locations, with 189 being tested in more than 1 year. The average RFLP (restriction fragment length polymorphism) based genetic distance index (DI) was 0.35. Midparent heterosis for grain yield ranged from −20 to 57% and high-parent heterosis from −22 to 47%. The correlation between RFLP-based estimates of genetic distance and COP was nonsignificant (−0.33). Coefficient of parentage was significantly correlated with heterosis for all traits in 1991 but not in other years. Genetic distance based on RFLPs scored in this study was not correlated with heterosis in any of the years tested. Further refinement of gene pools and new prediction methods will be required to facilitate the exploitation of genetic variability for hybrid wheat improvement. Key words : wheat, heterosis, genetic distance.

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Molybdenum incorporation in denitrifying Azospirillum brasilense Sp7

Canadian Journal of Microbiology ◽

10.1139/m92-171 ◽

1992 ◽

Vol 38 (10) ◽

pp. 1042-1047 ◽

Cited By ~ 2

Author(s):

Christian Chauret ◽

Wilfredo L. Barraquio ◽

Roger Knowles

Keyword(s):

Nitrate Reductase ◽

Key Words ◽

Nitrate Reduction ◽

Gel Electrophoresis ◽

Azospirillum Brasilense ◽

Paracoccus Denitrificans ◽

Free Medium ◽

Azospirillum Brasilense Sp7

Nondenaturating disc gel electrophoresis revealed that 99Mo was incorporated into the nitrate reductase of Azospirillum brasilense grown in the absence but not in the presence of tungstate. Under denitrifying conditions, A. brasilense grown in tungsten-free medium steadily accumulated 99Mo for 12 h. In contrast, Paracoccus denitrificans grown under the same conditions ceased uptake after 1 h. However, both bacteria were incapable of accumulating significant amounts of 99Mo in media containing 10 mM tungstate, even though nitrate was reduced by A. brasilense. Aerobically grown A. brasilense cells transported 99Mo more efficiently than anaerobically grown cells. Key words: Azospirillum brasilense, tungsten, molybdenum incorporation, nitrate reduction.

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Habitat differentiation between estuarine and inland Hibiscus tiliaceus L. (Malvaceae) as revealed by retrotransposon-based SSAP marker

Australian Journal of Botany ◽

10.1071/bt11041 ◽

2011 ◽

Vol 59 (6) ◽

pp. 515 ◽

Cited By ~ 2

Author(s):

Tian Tang ◽

Lian He ◽

Feng Peng ◽

Suhua Shi

Keyword(s):

Genetic Variation ◽

Gene Flow ◽

Genetic Distance ◽

Genetic Variance ◽

Genetic Relationships ◽

Principal Coordinate Analysis ◽

Geographical Isolation ◽

Tidal Zone ◽

Ecological Scale ◽

Habitat Differentiation

Hibiscus tiliaceus L. (Malvaceae) is a pantropical coastal tree that extends to the tidal zone. In this study, the retrotransposon sequence-specific amplified polymorphism (SSAP) technique was used in order to understand the genetic variation between four population pairs of H. tiliaceus from repeated estuarine and inland habitat contrasts in China. The estuarine populations were consistently more genetic variable compared with the inland ones, which may be attributed to extensive gene flow via water-drifted seeds and/or retrotransposon activation in stressful estuarine environments. An AMOVA revealed that 8.9% of the genetic variance could be explained by the habitat divergence within site, as compared with only 4.9% to geographical isolation between sites, which indicates significant habitat differentiation between the estuarine and inland populations. The estuarine populations were less differentiated (ΦST = 0.115) than the inland (ΦST = 0.152) implying frequent gene interchange in the former. Accordingly, the principal coordinate analysis of genetic distance between individuals revealed that genetic relationships are not fully consistent with the geographic association. These results suggest that despite substantial gene flow via sea-drifted seeds, habitat-related divergent selection could be one of the primary mechanisms that drive habitat differentiation in H. tiliaceus at a local ecological scale.

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