scholarly journals Genetic variation in relations in different goat populations of Bangladesh

1970 ◽  
Vol 39 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
MF Afroz ◽  
MO Faruque ◽  
SS Husain ◽  
JL Han ◽  
B Paul
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Key Words ◽  
Genetic Relation ◽  
Allele Number ◽  
Hilly Region ◽  
Different Populations

The genetic relation among different populations and breeds of goats in Bangladesh was studied using 15 microsatellite markers. A total of 181 goat samples- 36 from Black Bengal of western part (BBW), 39 from Black Bengal of central part (BBC), 40 from Black Bengal of Hilly region (BBH), 28 from exotic breeds (JAM) and 39 from crossbred (CRW) were genotyped. The allele number per locus ranged from 2 to 12. The average number of alleles per population was 5.69 ± 2.32, 5.38 ± 2.02, 5.23 ± 2.28, 6.08 ± 2.33, and 5.77 ± 2.35 for BBW, BBC, BBH, CRW and JAM, respectively. Heterozygosity was found in the range from 0.5049 ± 0.0222 (BBC) to 0.5751 ± 0.0262 (JAM). The genetic distance (DA) between BBC and JAM was the highest (0.0627) and that between JAM and CRW was the lowest (0.0037). In the phylogenetic dendrogram, BBC and BBH grouped in the same cluster, while CRW and JAM formed another cluster. BBW formed a third cluster between those two clusters. The closest genetic relation between BBW and CRW indicates the introgression of exotic genes occurred more in BBW than BBC and BBH. Key words: Bangladeshi goats; Microsatellite markers; Genetic variation; Dendrogram  DOI: http://dx.doi.org/10.3329/bjas.v39i1-2.9672 Bang. J. Anim. Sci. 2010, 39(1&2): 1-8

scholarly journals Genetic relationships in different sheep populations of Bangladesh based on microsatellite markers

1970 ◽  
Vol 7 (2) ◽  
pp. 291-294 ◽  
Author(s):  
MYA Khan ◽  
SS Husain ◽  
MR Alam ◽  
A Teneva ◽  
JL Han ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Microsatellite Marker ◽  
Genetic Relationships ◽  
Genetic Relation ◽  
Allele Number ◽  
Different Populations

The genetic variation and differentiation among different populations of sheep in Bangladesh were studied by using 18 microsatellite markers. A total of 140 samples- 37 from Bangladesh Central (BGC), 40 from Bangladesh East (BGE), 39 from Bangladesh North (BGN) and 24 from Garole (GAR) were genotyped. The allele number per locus ranged from 2 to 10. The average number of alleles per population was 5.50 ± 1.72, 5.33 ± 2.00, 6.39 ± 2.06 and 4.72 ± 1.71 for BGC, BGE, BGN and GAR respectively. Heterozygosity was found in the range from 0.5084±0.0187 (BGE) to 0.5810±0.0237 (GAR). The genetic distance (DA) between BGN and GAR was the highest (0.1071) and that between BGC and BGN was the lowest (0.0664). In the phylogenetic dendrogram, BGC and BGN grouped in the same cluster, while BGE and GAR formed another two separate clusters. The results indicate that all four sheep populations had rich genetic diversity and the Garole sheep (GAR) is considered as an independent breed in Bangladesh. Keywords: Sheep; Microsatellite marker; Genetic relation; Phylogenetic dendrogram DOI: 10.3329/jbau.v7i2.4736 J. Bangladesh Agril. Univ. 7(2): 291-294, 2009

scholarly journals KARAKTERISTIK GENETIK ENAM POPULASI IKAN NILEM (Osteochilus hasselti) DI JAWA BARAT

2016 ◽  
Vol 5 (2) ◽  
pp. 175 ◽  
Author(s):  
Mulyasari Mulyasari ◽  
Dinar Tri Soelistyowati ◽  
Anang Hari Kristanto ◽  
Irin Iriana Kusmini
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Data Base ◽  
Breeding Program ◽  
West Java ◽  
Allele Number ◽  
Specific Allele ◽  
Carp Fish

Penelitian karakteristik genetik populasi ikan nilem, Osteochilus hasselti di Jawa Barat dilakukan untuk mendapatkan data base sebagai langkah awal dalam melaksanakan program pemuliaan guna mempertahankan dan meningkatkan produksi dari ikan nilem di Jawa Barat. Tujuan penelitian ini adalah melakukan identifikasi genetik ikan nilem menggunakan metode RAPD dan menelusuri keragaman intra dan inter-populasi ikan nilem, Osteochilus hasselti di sentra budidaya yang terdapat di daerah Jawa Barat. Berdasarkan hasil penelitian, populasi Sumedang secara genetis memiliki keragaman paling tinggi dibandingkan dengan populasi lainnya serta alel spesifik yang tidak ditemukan pada populasi lain (1.100 bp). Sedangkan Sukabumi memiliki keragaman genetik dan jumlah alel yang paling rendah. Hubungan inter-populasi ikan nilem hijau di Jawa Barat tidak berbeda nyata di mana jarak genetik enam populasi ikan nilem tersebut berkisar antara 0,0153-0,1392.Research on genetic variation was done to conduct breeding program as the effort to maintain and increase the production of nilem carp fish at West Java. The aim of this study was to identify nilem carp genetically and to estimate the variation of the intra and inter population of nilem carp fish from West Java using RAPD methods. The result showed that Sumedang population had the highest genetic variation and had specific allele that cannot be found at other population (1,100 bp). But in contrast Sukabumi population had the lowest genetic variation and allele number. The inter- population relationship among fish from West Java were not significantly different. Genetic distance among population were between 0.0153-0.1392.

scholarly journals Molecular characterization of wheat (Triticum aestivum L.) genotypes through SSR markers

2012 ◽  
Vol 37 (3) ◽  
pp. 389-398 ◽  
Author(s):  
S Islam ◽  
MS Haque ◽  
RM Emon ◽  
MM Islam ◽  
SN Begum
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Allelic Diversity ◽  
Triticum Aestivum L ◽  
Allele Number ◽  
Average Gene

A study was undertaken to examine the genetic diversity of 12 wheat (Triticum aestivum L.) genotypes, using 4 simple sequence repeats (SSRs). A total of 10 alleles were found. Allele number per locus ranged from 2 to 4 with an average of 2.5. The polymorphic information content (PIC) values ranged from 0.2755 to 0.5411 with an average of 0.3839. The average gene diversity over all SSR loci for the 12 wheat genotypes was 0.4688, ranging from 0.3299 to 0.6042. Cluster analysis based on microsatellite allelic diversity discriminated the varieties into different clusters. Genetic diversity was the highest between variety Gourab and Akbar as well as Gourab and BAW-1064, showing a genetic distance value of 0.4697. The genetic distance was lowest between Balaka and Aghrani as well as Triticale and BAW-1036. Positive correlations were found between gene diversity, number of alleles, the allele size range and the types of repeat motif of microsatellite markers. It was found from this study that microsatellite markers could characterize and discriminate all of the genotypes. More primers should be used for saturation of different regions in further studies. Bangladesh J. Agril. Res. 37(3): 389-398, September 2012 DOI: http://dx.doi.org/10.3329/bjar.v37i3.12082

scholarly journals PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2017 ◽  
Vol 4 (1) ◽  
pp. 68
Author(s):  
M. H. Fariduddin Ath-thar ◽  
Intan Putriana ◽  
Dinar Tri Soelistyowati ◽  
Rudhy Gustiano
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Genetic Resources ◽  
Key Words ◽  
Genetic Relationship ◽  
Fragment Size ◽  
Random Amplified Polymorphic Dna ◽  
Tropical Species ◽  
Total Production ◽  
Culture Activity

Genotype performance of Tambakan, Helostoma temminckii (Cuvier, 1829) from Sumatera, Java and Kalimantan Polulation using Random Amplified Polymorphic DNA (RAPD)        Tambakan, kissing gourami (Helostoma temminckii) is well known as a freshwater tropical  species from Southeast Asia. In Indonesia, tambakan is an important commodity. However, total production of tambakan tends to decrease. Therefore, domestication has urgently to be done to solve the problem of Tambakan population. Three different tambakan populations from Sumatera, Java and Kalimantan were observed to find good genetic resources for culture activity. This study aimed to investigate the genetic variation of tambakan especially from West Java, Jambi, and South Kalimantan province using RAPD. The result showed that the highest polymorphism and heterozygosity was from South Kalimantan population among others. The three population observed had the fragment size ranged from 100- 2000 bp. The highest genetic distance was between Sumatera and Kalimantan (0,2877), while the lowest was between Kalimantan and Java (0,1961). Key words: Helostoma temminckii, genetic, heterozigosity, genetic relationship ABSTRAK        Ikan tambakan (Helostoma temminckii) adalah salah satu jenis ikan air tawar yang berasal dari wilayah tropis, tepatnya Asia Tenggara. Di Indonesia ikan tambakan merupakan salah satu ikan komoditas penting. Saat ini, jumlah produksi ikan tambakan cenderung menurun. Sehingga domestikasi sangat diperlukan untuk mengatasi masalah peneurunan populasi tambakan Tiga populasi ikan tambakan dari Sumatera, Jawa dan Kalimantan diobeservasi untuk mendapatkan sumber genetic terbaik yang dapat digunakan untuk kegiatan budidaya.. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetika populasi ikan tambakan Jambi, Jawa Barat dan Kalimantan Selatan menggunakan metode RAPD. Hasil menunjukkan bahwa polimorfisme dan heterosigositas tertinggi terdapat pada populasi ikan tambakan Kalimantan Selatan jika dibandingkan dengan populasi ikan tambakan lainnya. Ukuran fragmen DNA teramplifikasi berkisar antara 100-2000 bp. Jarak genetik paling jauh adalah antara populasi tambakan Sumatera dengan Kalimantan (0,2877), sedangkan jarak genetik terendah adalah tambakan Kalimantan dengan Jawa (0,1961).Kata kunci: Helostoma temminckii, genetik, heterosigositas, kekerabatan

scholarly journals Molecular characterization of Iranian dromedaries using microsatellite markers

2021 ◽  
Vol 69 (4) ◽  
pp. 321-330
Author(s):  
Mohammadreza Mohammadabadi ◽  
Mehrdad Ghasemi Meymandi ◽  
Mahdieh Montazeri ◽  
Volodymyr Afanasenko ◽  
Oleksandr Kalashnyk
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Camelus Dromedarius ◽  
Conservation Strategies ◽  
The North ◽  
Allele Number ◽  
Total Genetic Diversity ◽  
Hardy Weinberg Equilibrium ◽  
Different Populations ◽  
Future Direction

Considering the importance of maintaining the genetic diversity in native animals, this study conducted to analyse genetic diversity in dromedary populations in the north of Kerman province, Iran, using eight autosomal microsatellite markers. Eighty-one blood samples were collected from five different populations and DNA was extracted. The highest and the lowest allele number and effective alleles were shown in YWLL08 (21 and 4) and VOLP32 (14.97 and 3.11), respectively. The expected heterozygosity varied from 0.778 in Sahra-e Jahad population to 0.847 in Nogh population. The test for Hardy-Weinberg equilibrium showed significant deviations in most loci. The mean multilocus FST value (0.057) suggested that differentiation is moderate between populations. From total genetic diversity, only 6% were due to differentiation among populations, while the remaining 94% corresponded to differences among individuals within each population. The results of the current study indicated that the Camelus dromedarius populations in the north of Kerman province have a relativity high genetic variation and the data could be useful for designing the breeding strategies and conservation. The degree of variability demonstrated implies that studied populations are rich reservoirs of genetic diversity that must be preserved. A future direction to our study can be studying all of the Iranian Camelus dromedarius populations to better evaluate the level of inbreeding and establish the appropriate conservation strategies aimed to avoid losses of genetic diversity.

scholarly journals Genetic and Epigenetic Changes during the Upward Expansion of Deyeuxia angustifolia Kom. in the Alpine Tundra of the Changbai Mountains, China

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 291
Author(s):  
Biao Ni ◽  
Jian You ◽  
Jiangnan Li ◽  
Yingda Du ◽  
Wei Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Methylation ◽  
Genetic Variation ◽  
Soil Properties ◽  
Changbai Mountains ◽  
Epigenetic Variation ◽  
Geographical Distance ◽  
Mantel Tests ◽  
Epigenetic Diversity ◽  
Different Populations

Ecological adaptation plays an important role in the process of plant expansion, and genetics and epigenetics are important in the process of plant adaptation. In this study, genetic and epigenetic analyses and soil properties were performed on D. angustifolia of 17 populations, which were selected in the tundra zone on the western slope of the Changbai Mountains. Our results showed that the levels of genetic and epigenetic diversity of D. angustifolia were relatively low, and the main variation occurred among different populations (amplified fragment length polymorphism (AFLP): 95%, methylation sensitive amplification polymorphism (MSAP): 87%). In addition, DNA methylation levels varied from 23.36% to 35.70%. Principal component analysis (PCA) results showed that soil properties of different populations were heterogeneous. Correlation analyses showed that soil moisture, pH and total nitrogen were significantly correlated with genetic diversity of D. angustifolia, and soil temperature and pH were closely related to epigenetic diversity. Simple Mantel tests and partial Mantel tests showed that genetic variation significantly correlated with habitat or geographical distance. However, the correlation between epigenetic variation and habitat or geographical distance was not significant. Our results showed that, in the case of low genetic variation and genetic diversity, epigenetic variation and DNA methylation may provide a basis for the adaptation of D. angustifolia.

Genetic variation in three Chinese peat mosses (Sphagnum) based on microsatellite markers, with primer information and analysis of ascertainment bias

2008 ◽  
Vol 111 (2) ◽  
pp. 271-281 ◽  
Author(s):  
A. Jonathan Shaw ◽  
Tong Cao ◽  
Li-song Wang ◽  
Kjell Ivar Flatberg ◽  
Bergfrid Flatberg ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Microsatellite Markers ◽  
Ascertainment Bias

Development of microsatellite markers at the National Agrobiodiversity Center in Korea for the genetic assessment of underutilized crops

2014 ◽  
Vol 12 (S1) ◽  
pp. S125-S129
Author(s):  
Gi-An Lee ◽  
Sok-Young Lee ◽  
Ho-Sun Lee ◽  
Kyung-Ho Ma ◽  
Jae-Gyun Gwag ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Germplasm Management ◽  
Underutilized Crops ◽  
Crop Species ◽  
Closely Related Species ◽  
Polymorphic Loci ◽  
Allele Number ◽  
The Mean ◽  
Genetic Assessment

The RDA Genebank at the National Agrobiodiversity Center (NAAS, RDA, Republic of Korea) has conserved about 182,000 accessions in 1777 species and is working at preserving agricultural genetic resources for the conservation and sustainable utilization of genetic diversity. The detection of genetic variability in conserved resources is important for germplasm management, but the molecular evaluation tools providing genetic information are insufficient for underutilized crops, unlike those for major crops. In this regard, the Korean National Agrobiodiversity Center has been developing microsatellite markers for several underutilized crops. We designed 3640 primer pairs flanking simple sequence repeat (SSR) motifs for 6310 SSR clones in 21 crop species. Polymorphic loci were revealed in each species (7–36), and the mean ratio of polymorphic loci to all the loci tested was 12%. The average allele number was 5.1 (2.8–10.3) and the expected heterozygosity 0.51 (0.31–0.74). Some SSRs were transferable to closely related species, such as within the genera Fagopyrum and Allium. These SSR markers might be used for studying the genetic diversity of conserved underutilized crops.

scholarly journals Genetic Variation Among Colletotrichum graminicola Isolates from Four Hosts Using Isozyme Analysis

Plant Disease ◽  
2004 ◽  
Vol 88 (4) ◽  
pp. 402-406 ◽  
Author(s):  
B. J. Horvath ◽  
J. M. Vargas
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Geographic Origin ◽  
Creeping Bentgrass ◽  
Genetic Distances ◽  
Colletotrichum Graminicola ◽  
Annual Bluegrass ◽  
Close Relationship ◽  
Host Origin ◽  
Serious Disease

Anthracnose basal rot (ABR) is a serious disease of turfgrasses that is caused by the pathogen Colletotrichum graminicola. The relationships of isolates causing ABR on turfgrasses to those causing disease on important crop hosts (maize, sorghum) remain unresolved. Genetic variation among isolates from annual bluegrass, creeping bentgrass, maize, and sorghum was evaluated based on host origin and geographic origin. Isozymes were used to estimate the genetic variation of the isolates. Five enzyme systems comprising 16 alleles from 5 loci were used. Allele frequencies, genetic distance, and linkage disequilibrium values were calculated for isolates based on both host and geographic origin. Isolates from creeping bentgrass and annual bluegrass were the most closely related based on Nei's genetic distance, while isolates from maize and sorghum were the most distantly related, consistent with their known species-level relationship. Isolates from annual bluegrass and creeping bentgrass had different genetic distances to isolates from both maize and sorghum. Annual bluegrass isolates from different geographic regions had the smallest genetic distance values observed in this study, indicating a very close relationship regardless of geographic origin. Based on these data, it appears that host origin, not geographic origin, plays a more important role in the genetic diversity of these fungi.

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