Identification of CD133-, CD34- and KDR-positive cells in the bovine ovary: A new site of vascular wall resident endothelial progenitor cells

2012 ◽  
Vol 52 (2-4) ◽  
pp. 67-84 ◽  
Author(s):  
Kornelia Schoen ◽  
Ruth M. Hirschberg ◽  
Johanna Plendl ◽  
Sabine Kaessmeyer
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Vascular Wall ◽  
Endothelial Progenitor ◽  
Bovine Ovary

Potential implications of vascular wall resident endothelial progenitor cells

2007 ◽  
Vol 98 (11) ◽  
pp. 930-939 ◽  
Author(s):  
Derya Tilki ◽  
Hans-Peter Hohn ◽  
Ursula Gehling ◽  
Nerbil Kilic ◽  
Süleyman Ergün
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Tumor Therapy ◽  
Vascular Wall ◽  
Endothelial Progenitor ◽  
Vascular Regeneration ◽  
Predictive Role ◽  
Vascular Formation

SummaryA rapidly increasing body of data suggests an essential role of endothelial progenitor cells (EPCs) in vascular regeneration, formation of new vessels in cardiovascular diseases and also in tumor vasculogenesis. Moreover, recent data obtained from clinical studies with anti-angiogenic drugs in tumor therapy or with pro-angiogenic stimuli in ischemic disorders implicate a predictive role of the number of EPCs circulating in the peripheral blood in monitoring of these diseases. However, there is still some controversial data regarding the relevance of the EPCs in vascular formation depending on models used and diseases studied. One of the essential prerequisites for a better understanding of the whole contribution of EPCs to vascular formation in adult, a process called postnatal vasculogenesis, is to identify their exact sources. We could recently discover the existence of EPCs in a distinct zone of the vascular wall of large and middle sized adult blood vessels and showed that these cells are capable to differentiate into mature endothelial cells, to form capillary sprouts in arterial ring assay and to build vasa vasorumlike structures within the vascular wall. They also can be mobilized very rapidly from the vascular wall by tumor cells. This review will discuss the functional implications of these vascular wall resident endothelial progenitor cells (VW-EPCs) in relation to those of EPCs circulating in peripheral blood or derived from the bone marrow in cardiovascular and neoplastic diseases.

Pentoxifylline ameliorates chronic stress/high-fat diet-induced vascular wall disease: the role of circulating endothelial progenitor cells

2019 ◽  
Vol 392 (6) ◽  
pp. 669-683 ◽  
Author(s):  
Jolly Mounir William Labib ◽  
Sawsan Aboul-Fotouh ◽  
Mohamed Z. Habib ◽  
Mohamed Abd Elrahman Ahmed Mekawy ◽  
Kawthar A. Farrag ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Chronic Stress ◽  
High Fat Diet ◽  
Vascular Wall ◽  
High Fat ◽  
Endothelial Progenitor ◽  
Circulating Endothelial Progenitor Cells

scholarly journals Chronic periodontitis and ischemic heart disease: morphofunctional relation-ships

2020 ◽  
Vol 24 (4) ◽  
pp. 219-224
Author(s):  
Andrey V. Eremin ◽  
A. V. Lepilin ◽  
T. E. Lipatova ◽  
I. M. Kvetnoy
Keyword(s):  
Heart Disease ◽  
Endothelial Dysfunction ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Chronic Periodontitis ◽  
Vascular Wall ◽  
No Synthase ◽  
Endothelial Progenitor ◽  
Endothelial No Synthase ◽  
Cd34 Cells

The relationship between periodontal diseases and cardiovascular pathology is actively being studied. The clinical significance of tissue markers of endothelial dysfunction in acute or chronic periodontitis needs to be clarified. Materials and methods. The results of the examination of 65 patients with chronic generalized periodontitis (CP), 35 patients with chronic coronary heart disease (CHD), and 35 patients with combined pathology including CHD and CP were presented. Clinical instrumental examination, assessment of the functional state of the endothelium, immunohistochemical, and morphometric studies were performed. Results. Patients with moderate CP were characterized by functional changes in the endothelium, decreased expression of the vasodilating factor (e-NO-synthase), and endothelial progenitor cells (CD34+cells) in the vascular wall. In patients with CHD without periodontitis, there was also a decrease in the expression and optical density of endothelial NO-synthase and endothelial progenitor cell in the periodontal vessels. Conclusion. Apparently, changes in the expression of endothelial NO-synthase and endothelial progenitor cells (CD34+cells) in the vascular wall are generalized, and the gum can serve as a promising material for the early assessment of endothelial dysfunction.

scholarly journals Identification of Endothelial Progenitor Cells in the Corpus Cavernosum in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Jun Sik Lee ◽  
In Sang Hwang ◽  
Hyun-Suk Lee ◽  
Mi Eun Kim ◽  
Young-Woo Seo ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Flow Cytometric Analysis ◽  
Corpus Cavernosum ◽  
Vascular Wall ◽  
Sprague Dawley ◽  
Endothelial Progenitor ◽  
Sprague Dawley Rats ◽  
Normal Rat

The vascular wall resident progenitor cells seem to serve as a local reservoir of cells for vascular repair. It was hypothesized that the corpus cavernosum may contain vascular wall endothelial progenitor cells (EPCs). In this study, we investigated the identification and localization of EPCs in the corpus cavernosum in a rat model. Adult male Sprague-Dawley rats were used to isolate EPCs from corpora cavernosum. To verify the existence and localization of EPCs, EPC-specific markers (CD34, Flk-1, and VE-cadherin) were evaluated by flow cytometric analysis and confocal microscopy. The EPC markers were mainly expressed in the cavernosal sinusoidal endothelial space. EPC-marker-positive cells made up about 3.31% of the corpus cavernosum of normal rat by FACS analysis. As shown by confocal microscopy, CD34+/Flk-1+and CD34+/VE-cadherin+positive cells existed in the corpus cavernosum. Our findings imply that regulation of corpus cavernosal EPCs may be a new therapeutic strategy in the treatment of erectile dysfunction.

Hypoxia-induced proliferation of tissue-resident endothelial progenitor cells in the lung

2015 ◽  
Vol 308 (8) ◽  
pp. L746-L758 ◽  
Author(s):  
Rintaro Nishimura ◽  
Tetsu Nishiwaki ◽  
Takeshi Kawasaki ◽  
Ayumi Sekine ◽  
Rika Suda ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Fluorescent Protein ◽  
Vascular Endothelial Cells ◽  
Vascular Wall ◽  
Proliferative Potential ◽  
Vascular Endothelial ◽  
Endothelial Progenitor ◽  
Hypoxic Conditions

Exposure to hypoxia induces changes in the structure and functional phenotypes of the cells composing the pulmonary vascular wall from larger to most peripheral vessels. Endothelial progenitor cells (EPCs) may be involved in vascular endothelial repair. Resident EPCs with a high proliferative potential are found in the pulmonary microcirculation. However, their potential location, identification, and functional role have not been clearly established. We investigated whether resident EPCs or bone marrow (BM)-derived EPCs play a major role in hypoxic response of pulmonary vascular endothelial cells (PVECs). Mice were exposed to hypoxia. The number of PVECs transiently decreased followed by an increase in hypoxic animals. Under hypoxic conditions for 1 wk, prominent bromodeoxyuridine incorporation was detected in PVECs. Some Ki67-positive cells were detected among PVECs after 1 wk under hypoxic conditions, especially in the capillaries. To clarify the origin of proliferating endothelial cells, we used BM chimeric mice expressing green fluorescent protein (GFP). The percentage of GFP-positive PVECs was low and constant during hypoxia in BM-transplanted mice, suggesting little engraftment of BM-derived cells in lungs under hypoxia. Proliferating PVECs in hypoxic animals showed increased expression of CD34, suggesting hypoxia-induced gene expression and cell surface antigen of EPC or stem/progenitor cells markers. Isolated PVECs from hypoxic mice showed colony- and tube-forming capacity. The present study indicated that hypoxia could induce proliferation of PVECs, and the origin of these cells might be tissue-resident EPCs.

scholarly journals Identification of putative endothelial progenitor cells (CD34+CD133+Flk-1+) in endarterectomized tissue of patients with chronic thromboembolic pulmonary hypertension

2009 ◽  
Vol 296 (6) ◽  
pp. L870-L878 ◽  
Author(s):  
Weijuan Yao ◽  
Amy L. Firth ◽  
Richard S. Sacks ◽  
Aiko Ogawa ◽  
William R. Auger ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Progenitor Cell ◽  
Chronic Thromboembolic Pulmonary Hypertension ◽  
Cell Types ◽  
Vascular Wall ◽  
Potential Contribution ◽  
Endothelial Progenitor ◽  
Thromboembolic Pulmonary Hypertension

Chronic thromboembolic pulmonary hypertension (CTEPH) is characterized by a fibrotic thrombus persisting and obliterating the lumen of pulmonary arteries; its pathogenesis remains poorly defined. This study investigates a potential contribution for progenitor cell types in the development of vascular obliteration and remodeling in CTEPH patients. Endarterectomized tissue from patients undergoing pulmonary thromboendarterectomy was collected and examined for the structure and cellular composition. Our data show an organized fibrin network structure in unresolved thromboemboli and intimal remodeling in vascular wall tissues, characterized by smooth muscle α-actin (SM-αA)-positive cell proliferation in proximal regions (adjacent to thromboemboli) and neoangiogenesis/recanalization in distal regions (downstream from thromboemboli). Cells that are positively stained with CD34 and fetal liver kinase-1 (Flk-1) (CD34+Flk-1+) were identified in both the proximal and distal vascular tissues; a subpopulation of CD34+Flk-1+CD133+cells were further identified by immunostaining. Triple-positive cells are indicative of a population of putative endothelial progenitor cells or potential colony-forming units of endothelial cells. In addition, inflammatory cells (CD45+) and collagen-secreting cells (procollagen-1+) were detected in the proximal vascular wall. Some of the CD34+cells in CTEPH cells isolated from proximal regions were also positive for SM-αA. Our data indicate that putative progenitor cell types are present in the neointima of occluded vessels of CTEPH patients. It is possible that the microenvironment provided by thromboemboli may promote these putative progenitor cells to differentiate and enhance intimal remodeling.

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