scholarly journals Iron Hematoxylin Staining Method

2020 ◽  
Author(s):  
Keyword(s):  
Staining Method ◽  
Hematoxylin Staining

scholarly journals Evaluation of the Hematoxylin Staining Method for Detecting Wheat Tolerance to Alumlnum

1981 ◽  
Vol 31 (2) ◽  
pp. 152-160 ◽  
Author(s):  
Hiroko TAKAGI ◽  
Hyoji NAMAI ◽  
Kan-ichi MURAKAMI
Keyword(s):  
Staining Method ◽  
Hematoxylin Staining

scholarly journals Synaptophysin and Hematoxylin Staining Method

2020 ◽  
Author(s):  
Keyword(s):  
Staining Method ◽  
Hematoxylin Staining

AN IRON-ALUM-HEMATOXYLIN STAINING METHOD FOR MYELINATED FIBERS

1944 ◽  
Vol 3 (4) ◽  
pp. 419-420
Author(s):  
WILLIAM T. NIEMER
Keyword(s):  
Staining Method ◽  
Myelinated Fibers ◽  
Hematoxylin Staining ◽  
Iron Alum

Meiotic nuclear behavior and ascospore formation in five homothallic species of Neurospora

1978 ◽  
Vol 56 (7) ◽  
pp. 754-763 ◽  
Author(s):  
Namboori B. Raju
Keyword(s):  
Neurospora Crassa ◽  
Chromosome Numbers ◽  
Staining Method ◽  
Carnoy’S Solution ◽  
Nuclear Behavior ◽  
Polar Caps ◽  
Single File ◽  
Apical Pore ◽  
Hematoxylin Staining ◽  
Ascospore Formation

Meiotic nuclear behavior and chromosome numbers have been examined, using a propionic–iron–hematoxylin staining method, in Neurospora crassa and in five homothallic species, N. africana, N. dodgei, N. galapagosensis, N. lineolata and N. tcrricola. Ascus development, haploid chromosome numbers (n = 7), and morphology in all five homothallic species resemble the heterothallic N. crassa. The following observations have not been reported previously for any Neurospora species, although some have been described in other fungi. Most observations apply to all five homothallic species and to N. crassa except where otherwise indicated. (1) Chromosomes elongate considerably between karyogamy and the beginning of synapsis, and leptotene and zygotene stages can be identified. (2) The ascus tip flattens, and an apical pore begins to form during diplotene. (3) After telophase III, when all eight nuclei line up in single file, the nuclei are always arranged perpendicularly to the ascus wall with all spindle plaques on the same side. All nuclei then tilt relative to the ascus base with the spindle plaques at the lower end; consequently, the spores become obliquely arranged. (4) Ascospores of N. terricola are initially spindle shaped and occupy the entire ascus but later become compact and ovoid with spaces between them. (5) Maturing ascospores grow two to four times their initial compacted size. (6) In the young ascus, the nucleolus usually orients toward the base. (7) In N. terricola, the nucleolus is frequently hemispherical, especially in the young asci. (8) Half-moon-shaped polar caps are sometimes visible, one on each side of the prophase I nucleus; these are most prominent in N. terricola. (9) Cytological preparations are greatly improved if hydrolyzed perithecia are thoroughly washed in a Carnoy's solution containing chloroform.

scholarly journals A Simple Nuclear Contrast Staining Method for MicroCT-Based 3D Histology Using Lead(II) Acetate

2020 ◽  
Author(s):  
Brian Metscher
Keyword(s):  
3D Imaging ◽  
Staining Method ◽  
Prima Facie ◽  
Cell Nuclei ◽  
Staining Solution ◽  
X Ray ◽  
Hematoxylin Staining ◽  
Basic Protocol ◽  
Cell Components ◽  
Local Cell

ABSTRACTX-ray microtomography (microCT) enables histological-scale 3D imaging of many types of biological samples, but it has yet to rival traditional histology for differentiation of tissue types and cell components. This report presents prima facie results indicating that a simple lead(II) acetate staining solution can impart preferential X-ray contrast to cell nuclei. While not strictly selective for nuclei, the staining reflects local cell-density differences. It can be applied in a single overnight treatment and does not require hematoxylin staining or drying of the sample. The stain is removable with EDTA, and it may enhance early calcifications. A basic protocol is given as a guide for further testing and optimisation.

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Enzyme histochemical application and backscattered electron imaging to visualize the distribution of lymphatic capillaries

1990 ◽  
Vol 48 (3) ◽  
pp. 880-881
Author(s):  
Seiji Kato
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Blood Capillaries ◽  
Backscattered Electron Imaging ◽  
Histochemical Observation ◽  
Backscattered Electron ◽  
Double Staining Method ◽  
Electron Imaging ◽  
Cryostat Sections

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

A Fast Histochemical Staining Method to Identify Hyaline Droplets in the Rat Kidney

2003 ◽  
Vol 31 (4) ◽  
pp. 462-464 ◽  
Author(s):  
Eveline P. C. T. de Rijk ◽  
Wilma T. M. Ravesloot ◽  
Yvonne Wijnands ◽  
Eric van Esch
Keyword(s):  
Staining Method ◽  
Rat Kidney ◽  
Histochemical Staining

Whey Acidic Protein Staining Method

2020 ◽  
Author(s):  
Keyword(s):  
Staining Method ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Protein Staining
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