scholarly journals A Simple Nuclear Contrast Staining Method for MicroCT-Based 3D Histology Using Lead(II) Acetate

2020 ◽  
Author(s):  
Brian Metscher
Keyword(s):  
3D Imaging ◽  
Staining Method ◽  
Prima Facie ◽  
Cell Nuclei ◽  
Staining Solution ◽  
X Ray ◽  
Hematoxylin Staining ◽  
Basic Protocol ◽  
Cell Components ◽  
Local Cell

ABSTRACTX-ray microtomography (microCT) enables histological-scale 3D imaging of many types of biological samples, but it has yet to rival traditional histology for differentiation of tissue types and cell components. This report presents prima facie results indicating that a simple lead(II) acetate staining solution can impart preferential X-ray contrast to cell nuclei. While not strictly selective for nuclei, the staining reflects local cell-density differences. It can be applied in a single overnight treatment and does not require hematoxylin staining or drying of the sample. The stain is removable with EDTA, and it may enhance early calcifications. A basic protocol is given as a guide for further testing and optimisation.

scholarly journals 3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

10.3791/60251 ◽  
2019 ◽  
Author(s):  
Madleen Busse ◽  
Mark Müller ◽  
Melanie A. Kimm ◽  
Simone Ferstl ◽  
Sebastian Allner ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Soft Tissue ◽  
3D Imaging ◽  
Staining Method ◽  
Specific Staining ◽  
Tissue Samples ◽  
X Ray

X-Ray Tomography for Electronic Packages

2000 ◽  
Author(s):  
Deepak Goyal
Keyword(s):  
3D Imaging ◽  
Development Time ◽  
Analytical Techniques ◽  
Electronic Packages ◽  
X Ray ◽  
Imaging Capability ◽  
Imaging Results ◽  
Key Drivers ◽  
Analytical Tools ◽  
Non Destructive

Abstract Next generation assembly/package development challenges are primarily increased interconnect complexity and density with ever shorter development time. The results of this trend present some distinct challenges for the analytical tools/techniques to support this technical roadmap. The key challenge in the analytical tools/techniques is the development of non-destructive imaging for improved time to information. This paper will present the key drivers for the non-destructive imaging, results of literature search and evaluation of key analytical techniques currently available. Based on these studies requirements of a 3D imaging capability will be discussed. Critical breakthroughs required for development of such a capability are also summarized.

Analyzing the Impact of X-Ray Tomography on the Reliability of Integrated Circuits

2015 ◽  
Author(s):  
Halit Dogan ◽  
Md Mahbub Alam ◽  
Navid Asadizanjani ◽  
Sina Shahbazmohamadi ◽  
Domenic Forte ◽  
...  
Keyword(s):  
Integrated Circuits ◽  
Integrated Circuit ◽  
3D Imaging ◽  
Three Dimensional ◽  
Serial Sectioning ◽  
Promising Technique ◽  
Flash Memories ◽  
X Ray ◽  
3D Information ◽  
The Impact

Abstract X-ray tomography is a promising technique that can provide micron level, internal structure, and three dimensional (3D) information of an integrated circuit (IC) component without the need for serial sectioning or decapsulation. This is especially useful for counterfeit IC detection as demonstrated by recent work. Although the components remain physically intact during tomography, the effect of radiation on the electrical functionality is not yet fully investigated. In this paper we analyze the impact of X-ray tomography on the reliability of ICs with different fabrication technologies. We perform a 3D imaging using an advanced X-ray machine on Intel flash memories, Macronix flash memories, Xilinx Spartan 3 and Spartan 6 FPGAs. Electrical functionalities are then tested in a systematic procedure after each round of tomography to estimate the impact of X-ray on Flash erase time, read margin, and program operation, and the frequencies of ring oscillators in the FPGAs. A major finding is that erase times for flash memories of older technology are significantly degraded when exposed to tomography, eventually resulting in failure. However, the flash and Xilinx FPGAs of newer technologies seem less sensitive to tomography, as only minor degradations are observed. Further, we did not identify permanent failures for any chips in the time needed to perform tomography for counterfeit detection (approximately 2 hours).

scholarly journals Spectral Tomography for 3D Element Detection and Mineral Analysis

Minerals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 598
Author(s):  
Jose R. A. Godinho ◽  
Gabriel Westaway-Heaven ◽  
Marijn A. Boone ◽  
Axel D. Renno
Keyword(s):  
Energy Spectrum ◽  
3D Imaging ◽  
X Rays ◽  
3D Images ◽  
Characterization Methods ◽  
X Ray ◽  
Additional Information ◽  
Mineral Phases ◽  
Spectral Computed Tomography

This paper demonstrates the potential of a new 3D imaging technique, Spectral Computed Tomography (sp-CT), to identify heavy elements inside materials, which can be used to classify mineral phases. The method combines the total X-ray transmission measured by a normal polychromatic X-ray detector, and the transmitted X-ray energy spectrum measured by a detector that discriminates between X-rays with energies of about 1.1 keV resolution. An analysis of the energy spectrum allows to identify sudden changes of transmission at K-edge energies that are specific of each element. The additional information about the elements in a phase improves the classification of mineral phases from grey-scale 3D images that would be otherwise difficult due to artefacts or the lack of contrast between phases. The ability to identify the elements inside the minerals that compose ore particles and rocks is crucial to broaden the application of 3D imaging in Earth sciences research and mineral process engineering, which will represent an important complement to traditional 2D imaging mineral characterization methods. In this paper, the first applications of sp-CT to classify mineral phases are showcased and the limitations and further developments are discussed.

scholarly journals 3D Imaging in Unilateral Primary Pulmonary Hypoplasia in an Adult: A Case Report

2011 ◽  
Vol 2011 ◽  
pp. 1-6
Author(s):  
Aristida Georgescu ◽  
Crinu Nuta ◽  
Simona Bondari
Keyword(s):  
3D Imaging ◽  
Pulmonary Hypoplasia ◽  
Left Lung ◽  
Left Lobe ◽  
X Rays ◽  
X Ray ◽  
Female Smoker ◽  
Multidetector Computer Tomography ◽  
Biological Tests ◽  
Chest X Ray

Unilateral primary pulmonary hypoplasia is rare in adulthood (UPHA); it is characterized by a decreased number of bronchial segmentation and decreased/absent alveolar air space. Classical chest X-ray may be confusing, and the biological tests are unspecific. We present a case of UPHA in a 60-year-old female, smoker, with 3 term normal deliveries, who presented with late recurrent pneumonias and bronchiectasis-type symptomathology, arterial hypertension, and obesity. Chest X-rays revealed opacity in the left lower pulmonary zone, an apparent hypoaerated upper left lobe and left deviation of the mediastinum. Preoperatory multidetector computer tomography (MDCT) presented a small retrocardiac left lung with 5-6 bronchial segmentation range and cystic appearance. After pneumonectomy the gross specimen showed a small lung with multiple bronchiectasis and small cysts, lined by hyperplasic epithelium, surrounded by stromal fibrosclerosis. We concluded that this UPHA occurred in the 4–7 embryonic weeks, and the 3D MDCT reconstructions offered the best noninvasive diagnosis.

scholarly journals Nuclear incorporation of iron during the eukaryotic cell cycle

2016 ◽  
Vol 23 (6) ◽  
pp. 1490-1497 ◽  
Author(s):  
Ian Robinson ◽  
Yang Yang ◽  
Fucai Zhang ◽  
Christophe Lynch ◽  
Mohammed Yusuf ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Fluorescence Microscopy ◽  
Phase Contrast ◽  
Eukaryotic Cell ◽  
Inhomogeneous Distribution ◽  
Cell Nuclei ◽  
X Ray ◽  
Microscopy Images ◽  
Different Levels

Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X-ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed.

scholarly journals Three-dimensional imaging of hold baggage for airport security

2014 ◽  
Vol XL-5 ◽  
pp. 331-336
Author(s):  
S. Kolokytha ◽  
R. Speller ◽  
S. Robson
Keyword(s):  
3D Imaging ◽  
Imaging System ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
Handling Time ◽  
Cost Effective ◽  
Airport Security ◽  
X Ray ◽  
Common System ◽  
Close Range

This study describes a cost-effective check-in baggage screening system, based on "on-belt tomosynthesis" (ObT) and close-range photogrammetry, that is designed to address the limitations of the most common system used, conventional projection radiography. The latter's limitations can lead to loss of information and an increase in baggage handling time, as baggage is manually searched or screened with more advanced systems. This project proposes a system that overcomes such limitations creating a cost-effective automated pseudo-3D imaging system, by combining x-ray and optical imaging to form digital tomograms. Tomographic reconstruction requires a knowledge of the change in geometry between multiple x-ray views of a common object. This is uniquely achieved using a close range photogrammetric system based on a small network of web-cameras. This paper presents the recent developments of the ObT system and describes recent findings of the photogrammetric system implementation. Based on these positive results, future work on the advancement of the ObT system as a cost-effective pseudo-3D imaging of hold baggage for airport security is proposed.

scholarly journals Quantifying phosphoric acid in high-temperature polymer electrolyte fuel cell components by X-ray tomographic microscopy

2014 ◽  
Vol 21 (6) ◽  
pp. 1319-1326 ◽  
Author(s):  
S. H. Eberhardt ◽  
F. Marone ◽  
M. Stampanoni ◽  
F. N. Büchi ◽  
T. J. Schmidt
Keyword(s):  
High Temperature ◽  
Fuel Cell ◽  
Phosphoric Acid ◽  
Polymer Electrolyte ◽  
Diffusion Layer ◽  
Gas Diffusion Layer ◽  
Gas Diffusion ◽  
X Ray ◽  
Wide Range ◽  
Cell Components

Synchrotron-based X-ray tomographic microscopy is investigated for imaging the local distribution and concentration of phosphoric acid in high-temperature polymer electrolyte fuel cells. Phosphoric acid fills the pores of the macro- and microporous fuel cell components. Its concentration in the fuel cell varies over a wide range (40–100 wt% H3PO4). This renders the quantification and concentration determination challenging. The problem is solved by using propagation-based phase contrast imaging and a referencing method. Fuel cell components with known acid concentrations were used to correlate greyscale values and acid concentrations. Thus calibration curves were established for the gas diffusion layer, catalyst layer and membrane in a non-operating fuel cell. The non-destructive imaging methodology was verified by comparing image-based values for acid content and concentration in the gas diffusion layer with those from chemical analysis.

scholarly journals 3D imaging of proximal caries in posterior teeth using optical coherence tomography

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yasushi Shimada ◽  
Michael F. Burrow ◽  
Kazuyuki Araki ◽  
Yuan Zhou ◽  
Keiichi Hosaka ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diagnostic Accuracy ◽  
3D Imaging ◽  
Characteristic Curve ◽  
Weighted Kappa ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Posterior Teeth ◽  
Proximal Caries ◽  
X Ray

Abstract Optical coherence tomography (OCT) can create cross-sectional images of tooth without X-ray exposure. This study aimed to investigate the diagnostic accuracy of 3D imaging of OCT for proximal caries in posterior teeth. Thirty-six human molar teeth with 51 proximal surfaces visibly 6 intact, 16 slightly demineralized, and 29 distinct carious changes were mounted to take digital radiographs and 3D OCT images. The sensitivity, specificity and area under the receiver operating characteristic curve (AUC) for the diagnosis of enamel caries and dentin caries were calculated to quantify the diagnostic ability of 3D OCT in comparison with digital radiography. Diagnostic accuracy was evaluated by the agreement with histology using weighted Kappa. OCT showed significantly higher sensitivity, AUC and Kappa values than radiography. OCT can be a safer option for the diagnosis of proximal caries in posterior teeth that can be applied to the patients without X-ray exposure.

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