Nitric oxide as an indicator for assessing the resistance and susceptibility of cattle to leukemia

Abstract. The role of allelic variability of inducible nitric oxide synthase (iNOS) is significant in the study of the resistance and susceptibility of animals to leukemia infection. After analyzing the literature data, it can be stated that in the iNOS gene, allele A (with genotype AA) is responsible for resistance to the leukemia virus, and allele B (with genotype BB) is responsible for susceptibility. This is due to the frequency of occurrence of alleles and their genotypes of the polymorphic marker AN13-1 of the inducibeal nitric oxide synthase gene. The iNOS gene is capable of producing a large amount of nitric oxide, compared to other isoforms. In turn, nitric oxide causes death or can stop the growth of pathogenic microorganisms, including viruses. The purpose of this work is to further study nitric oxide as an indicator for determining the resistance and susceptibility of animals to leukemia, as well as the selection of specific primers for PCR-PDRF used in genotyping. Methods. The iNOS gene sequence was analyzed and a pair of specific primers were selected and synthesized using the Vector NTI program. Scientific novelty of this work lies in the fact that we have selected specific primers that are important for the analysis of cattle genotyping by allelic variants of the polymorphic marker AH13-1 of the iNOS gene. Results. Based on this work, a pair of primers iNOSF_new and iNOSR_new, with a calculated annealing temperature of 52 °C, were selected and synthesized, giving an amplicon with a length of 186 bp. The amplicon contains a polymorphic site that distinguishes the A and B alleles. During PCR-RFLP, the following genotype-specific fragments are formed: AA-47/139 bp; AB -186/139/47 bp; BB-186 bp.

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Role of neuronal nitric oxide synthase in gastric hypermotility induced by intracisternal TRH analog, RX 77368, in anesthetized rats

Gastroenterology ◽

10.1016/s0016-5085(01)82651-2 ◽

2001 ◽

Vol 120 (5) ◽

pp. A533-A534

Author(s):

K KANAMOTO ◽

K KAWAKUBO ◽

D ADELSON ◽

Y TACHE

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Neuronal Nitric Oxide Synthase ◽

Anesthetized Rats ◽

Oxide Synthase

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Role of Intronic MicroRNA in The Regulation of Endothelial Nitric Oxide Synthase Expression and The Proliferation of Endothelial Cells*

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ◽

10.3724/sp.j.1206.2009.00755 ◽

2010 ◽

Vol 37 (7) ◽

pp. 747-753 ◽

Cited By ~ 4

Author(s):

Li-Mei YAN ◽

Jian-Yong WU ◽

Xiao-Hua YU ◽

Jing-Ou XU ◽

He-Sheng OU

Keyword(s):

Nitric Oxide ◽

Endothelial Cells ◽

Nitric Oxide Synthase ◽

Endothelial Nitric Oxide Synthase ◽

Oxide Synthase ◽

Endothelial Nitric Oxide

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Vasculoprotective Role of Inducible Nitric Oxide Synthase at Inflammatory Coronary Lesions Induced by Chronic Treatment With Interleukin-1β in Pigs in Vivo

Circulation ◽

10.1161/01.cir.96.9.3104 ◽

1997 ◽

Vol 96 (9) ◽

pp. 3104-3111 ◽

Cited By ~ 27

Author(s):

Yoshihiro Fukumoto ◽

Hiroaki Shimokawa ◽

Toshiyuki Kozai ◽

Toshiaki Kadokami ◽

Kouichi Kuwata ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Inducible Nitric Oxide Synthase ◽

Chronic Treatment ◽

Interleukin 1Β ◽

Coronary Lesions ◽

Oxide Synthase ◽

Inducible Nitric Oxide

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Neuronal nitric oxide synthase and systemic vasodilation in rats with cirrhosis

AJP Renal Physiology ◽

10.1152/ajprenal.2000.279.6.f1110 ◽

2000 ◽

Vol 279 (6) ◽

pp. F1110-F1115 ◽

Cited By ~ 50

Author(s):

Lieming Xu ◽

Ethan P. Carter ◽

Mamiko Ohara ◽

Pierre-Yves Martin ◽

Boris Rogachev ◽

...

Keyword(s):

Nitric Oxide ◽

Liver Cirrhosis ◽

Nitric Oxide Synthase ◽

Control Treatment ◽

Sodium Balance ◽

Hyperdynamic Circulation ◽

Molecular Approaches ◽

Advanced Liver Cirrhosis ◽

Oxide Synthase

Cirrhosis is typically associated with a hyperdynamic circulation consisting of low blood pressure, low systemic vascular resistance (SVR), and high cardiac output. We have recently reported that nonspecific inhibition of nitric oxide synthase (NOS) with nitro-l-arginine methyl ester reverses the hyperdynamic circulation in rats with advanced liver cirrhosis induced by carbon tetrachloride (CCl4). Although an important role for endothelial NOS (eNOS) is documented in cirrhosis, the role of neuronal NOS (nNOS) has not been investigated. The present study was carried out to specifically investigate the role of nNOS during liver cirrhosis. Specifically, physiological, biochemical, and molecular approaches were employed to evaluate the contribution of nNOS to the cirrhosis-related hyperdynamic circulation in CCl4-induced cirrhotic rats with ascites. Cirrhotic animals had a significant increase in water and sodium retention. In the aorta from cirrhotic animals, both nNOS protein expression and cGMP concentration were significantly elevated compared with control. Treatment of cirrhotic rats for 7 days with the specific nNOS inhibitor 7-nitroindazole (7-NI) normalized the low SVR and mean arterial pressure, elevated cardiac index, and reversed the positive sodium balance. Increased plasma arginine vasopressin concentrations in the cirrhotic animals were also repressed with 7-NI in association with diminished water retention. The circulatory changes were associated with a reduction in aortic nNOS expression and cGMP. However, 7-NI treatment did not restore renal function in cirrhotic rats (creatinine clearance: 0.76 ± 0.03 ml · min−1· 100 g body wt−1in cirrhotic rats vs. 0.79 ± 0.05 ml · min−1· 100 g body wt−1in cirrhotic rats+7-NI; P NS.). Taken together, these results indicate that nNOS-derived NO contributes to the development of the hyperdynamic circulation and fluid retention in cirrhosis.

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