scholarly journals “COMPARATIVE EVALUATION OF EFFECT ON DENTINAL TUBULE OCCLUSION BY THREE DIFFERENT DESENSITIZING DENTIFICES: A SEM STUDY”

2021 ◽  
Vol 5 (11) ◽  
Author(s):  
Aman Mallick ◽  
Gaurav Malhotra ◽  
Pradeep Shukla ◽  
Prerna Kataria ◽  
C. S Joshi
Keyword(s):  
Strontium Chloride ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentine Hypersensitivity ◽  
Study Results ◽  
Sem Study ◽  
Group 3 ◽  
Group 2 ◽  
Tubule Occlusion

Introduction: Dentine hypersensitivity (DH) occurs on exposed dentine and is dependent on the patency of dentinal tubules. This study compared the effectiveness of toothpaste containing Strontium Chloride, Novamine, Pro-Arginine in occluding dentine tubules along with comparision of those. Materials and Method: 45 freshly extracted teeth were randomly divided (15 each) into 3 groups: Group 1 15 Teeth treated with toothpaste containing Strontium Chloride. Group 2 15 Teeth treated with toothpaste containing Novamine Group 3 15 Teeth treated with toothpaste containing Pro-Arginine. Each specimen was brushed with the dentifrice slurries and examined under SCM. Result: The mean of occluded dentinal tubules on the dentin surface after brushing with three dentifrices were significant among them Pro-arginine group showed maximum occlusion. Conclusion: The present in vitro SEM study results revealed that:  All the experimental agents– Strontium chloride, novamin, Pro- Argenine were effective in occluding dentinal tubules & the percentage (%) of occluded tubules was found to be highest for Pro- Argenine as compared to the other groups. Keywords: SCM, DH

SEM Analysis of Residual Dentin Surface in Primary Teeth Using Different Chemomechanical Caries Removal Agents

2017 ◽  
Vol 41 (4) ◽  
pp. 289-293
Author(s):  
Rachna Thakur ◽  
Sandya Devi S Patil ◽  
Anil Kush ◽  
K Madhu
Keyword(s):  
In Vitro Study ◽  
Sem Analysis ◽  
Dentinal Tubules ◽  
Carious Lesions ◽  
Sem Study ◽  
Caries Removal ◽  
Group 2 ◽  
Caries Excavation ◽  
Group 1

Background: The purpose of this in vitro study was to analyze the residual dentinal surfaces following caries removal using two chemomechanical methods (Papacarie Duo and Carie Care), by scanning electron microscopy (SEM). Study design: Twenty extracted primary molars with active occlusal carious lesions were randomly assigned two groups depending on the CMCR agent used for the caries excavation – Group 1 – with Papacarie Duo and Group – 2 with Carie Care. After the caries excavation, the specimens were subjected to SEM analysis. Results: Though both the agents showed the minimal smear layer with the patent dentinal tubules, Carie care showed patent dentinal tubules with a clearly exposed peritubular and intertubular collagen network. Conclusion: Carie Care treated surface exhibited better surface morphology of residual dentin.

Occlusion Effect of Dentinal Tubules of the Dentifrice Containing Nano-Sized Carbonate Apatite

2007 ◽  
Vol 342-343 ◽  
pp. 821-824
Author(s):  
Su Young Lee ◽  
Sun Ok Jang ◽  
H.K. Kwon ◽  
Young Sik Cho ◽  
B.I. Kim
Keyword(s):  
Strontium Chloride ◽  
Carbonate Apatite ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentin Surface ◽  
Carbonated Apatite ◽  
Group 4 ◽  
Significant Difference ◽  
Group 1

Exposed dentine with patent tubules allows the movement of the tubule fluid leading to dentine sensitivity. An occlusion of patent dentinal tubules can effectively reduce the state of dentine hypersensitivity. Strontium chloride (SrCl2) has been known as a representative component of a desensitizing dentifrice. Recently, a desensitizing dentifrice containing nano-sized Carbonated Apatites (n-CAPs) was released onto the market. The aim of this in vitro study was to evaluate the occlusion effects of dentinal tubules in dentifrices containing several concentrations of the n-CAPs. One hundred human dentine specimens were embedded into a Teflon mold and ground with silicone carbide papers to expose the dentin surface. The dentin surface was etched with 37% phosphoric acid for 60 seconds and washed ultrasonically for 1 hour. The dentifrices were classified into five groups: experimental dentifrices Group 1: n-CAPs 5%, group 2: n-CAPs 15%, group 3: n- CAPs 0% and group 4: n-CAPs 30%, and the control dentifrice group 5: SrCl2 10%. The dentifrice slurries were prepared as 20 grams of dentifrice in 80 ml of distilled water. Fifty specimens were brushed with the dentifrice slurries using a V8 Cross Brushing Machine. Tooth brushing was performed 5,000 times using a back-and–forth stroke. Images showing the sizes of the dentinal tubules were taken by scanning electron microscopy (×3000) and the changes in the tubule sizes were analyzed using a digital analyzer. The n-CAPs used in this study had a high solubility and affinity to bone minerals. In addition, the n-CAPs were well deposited on patent dentinal tubules. The group 1 dentifrice (n-CAPs 5% and silica 25%) showed the highest dentinal tubule occlusion, and there was a statistically significant difference between group 4 and the control (p<0.05). Overall, the dentifrice containing 5% nano carbonated apatite and 25% silica is the most effective in occluding dentinal tubules.

Occlusion Effect of Dentinal Tubules of the Dentifrice Containing Sodium Metasilicate

2007 ◽  
Vol 330-332 ◽  
pp. 1429-1432
Author(s):  
Su Young Lee ◽  
S.O. Jang ◽  
Hae Sun Kim ◽  
Jai Hyuk Choi ◽  
H.K. Kwon ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Metasilicate ◽  
Control Group ◽  
Strontium Chloride ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
The Difference ◽  
Silicone Carbide ◽  
Occlusion Effect

Exposed dentine with patent tubules allows the movement of tubule fluid leading to dentine sensitivity. Occlusion of patent dentinal tubules effectively reduces the state of dentine hypersensitivity. Strontium chloride (SrCl2) has been well known as a component of desensitizing dentifrice. Recently, new dentifrice containing sodium metasilicate was on the market for desensitizing dentifrice. The aim of this in vitro study was to compare the occlusion effect of dentinal tubule between the sodium metasilicate and the existing desensitizing dentifrices. Sixtyfour human dentine specimens were embedded into a Teflon mold and were grinded with the use of silicone carbide papers to expose the surface of dentin. The dentin surface was etched with 37% phosphoric acid for 60 seconds and was treated with ultrasonic washing for 1 hour. The dentifrices used in the study are classified into four groups: G1-dentifrice containing Sodium metasilicate, G2- dentifrice containing Strontium chloride, G3-dentifrice containing Hydroxyapatite and G4-BSI reference dentifrice (control group). Toothpaste slurries were prepared as 20 gram of toothpaste in 80 ml of distilled water. Thirty-two specimens were brushed with the toothpaste slurries using V8 Cross Brushing Machine. Tooth-brushing was performed 5,000 times using a back-and–forth stroke. The pictures of the sizes of dentinal tubules were taken by scanning electron microscopy (×3000) and changes of tubule sizes were analyzed by digital analysis. The results showed that the difference of tubule size in descending order: G1-3.30±1.80, G2-2.82±1.73, G3-1.49±1.14, G4- 1.04±0.94. The dentifrice containing Sodium metasilicate (22.5%) showed the highest dentinal tubule occlusion. Moreover, the dentifrice containing Sodium metasilicate statistically significantly increased occlusion of dentinal tubule as compared to BSI reference dentifrice (p<0.05). Thus, new desensitizing dentifrice containing sodium metasilicate was more effective than existing desensitizing dentifrice on occlusion of dentin tubules.

scholarly journals THU0227 CAFFEINE INTAKE MODULATES DISEASE ACTIVITY AND CYTOKINES LEVELS IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 340.2-341
Author(s):  
V. Orefice ◽  
F. Ceccarelli ◽  
C. Barbati ◽  
R. Lucchetti ◽  
G. Olivieri ◽  
...  
Keyword(s):  
Disease Activity ◽  
Lupus Erythematosus ◽  
Caffeine Intake ◽  
Caffeine Consumption ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
The Impact ◽  
Group 1

Background:Systemic lupus erythematosus (SLE) is an autoimmune disease mainly affecting women of childbearing age. The interplay between genetic and environmental factors may contribute to disease pathogenesis1. At today, no robust data are available about the possible contribute of diet in SLE. Caffeine, one of the most widely consumed products in the world, seems to interact with multiple components of the immune system by acting as a non-specific phosphodiesterase inhibitor2.In vitrodose-dependent treatment with caffeine seems to down-regulate mRNA levels of key inflammation-related genes and similarly reduce levels of different pro-inflammatory cytokines3.Objectives:We evaluated the impact of caffeine consumption on SLE-related disease phenotype and activity, in terms of clinimetric assessment and cytokines levels.Methods:We performed a cross-sectional study, enrolling consecutive patients and reporting their clinical and laboratory data. Disease activity was assessed by SLE Disease Activity Index 2000 (SLEDAI-2k)4. Caffeine intake was evaluated by a 7-day food frequency questionnaire, including all the main sources of caffeine. As previously reported, patients were divided in four groups according to the daily caffeine intake: <29.1 mg/day (group 1), 29.2-153.7 mg/day (group 2), 153.8-376.5 mg/day (group 3) and >376.6 mg/day (group 4)5. At the end of questionnaire filling, blood samples were collected from each patient to assess cytokines levels. These were assessed by using a panel by Bio-Plex assays to measure the levels of IL-6, IL-10, IL-17, IL-27, IFN-γ, IFN-α and Blys.Results:We enrolled 89 SLE patients (F/M 87/2, median age 46 years, IQR 14; median disease duration 144 months, IQR 150). The median intake of caffeine was 195 mg/day (IQR 160.5). At the time of the enrollment, 8 patients (8.9%) referred a caffeine intake < 29.1 mg/day (group 1), 27 patients (30.3%) between 29.2 and 153.7 mg/day (group 2), 45 patients (51%) between 153.8 and 376.5 mg/day (group 3) and 9 patients (10.1%) >376.6 mg/day (group 4). A negative correlation between the levels of caffeine and disease activity, evaluated with SLEDAI-2K, was observed (p=0.01, r=-0.26). By comparing the four groups, a significant higher prevalence of lupus nephritis, neuropsychiatric involvement, haematological manifestations, hypocomplementemia and anti-dsDNA positivity was observed in patients with less intake of caffeine (figure 1 A-E). Furthermore, patients with less intake of caffeine showed a significant more frequent use of glucocorticoids [group 4: 22.2%,versusgroup 1 (50.0%, p=0.0001), group 2 (55.5%, p=0.0001), group 3 (40.0%, p=0.009)]. Moving on cytokines analysis, a negative correlation between daily caffeine consumption and serum level of IFNγ was found (p=0.03, r=-0.2) (figure 2A); furthermore, patients with more caffeine intake showed significant lower levels of IFNα (p=0.02, figure 2B), IL-17 (p=0.01, figure 2C) and IL-6 (p=0.003, figure 2D).Conclusion:This is the first report demonstrating the impact of caffeine on SLE disease activity status, as demonstrated by the inverse correlation between its intake and both SLEDAI-2k values and cytokines levels. Moreover, in our cohort, patients with less caffeine consumption seems to have a more severe disease phenotype, especially in terms of renal and neuropsychiatric involvement. Our results seem to suggest a possible immunoregulatory dose-dependent effect of caffeine, through the modulation of serum cytokine levels, as already suggested byin vitroanalysis.References:[1]Kaul et alNat. Rev. Dis. Prim.2016; 2. Aronsen et alEurop Joul of Pharm2014; 3. Iris et alClin Immun.2018; 4. Gladman et al J Rheumatol. 2002; 5. Mikuls et alArth Rheum2002Disclosure of Interests:Valeria Orefice: None declared, Fulvia Ceccarelli: None declared, cristiana barbati: None declared, Ramona Lucchetti: None declared, Giulio Olivieri: None declared, enrica cipriano: None declared, Francesco Natalucci: None declared, Carlo Perricone: None declared, Francesca Romana Spinelli Grant/research support from: Pfizer, Consultant of: Novartis, Gilead, Lilly, Sanofi, Celgene, Speakers bureau: Lilly, cristiano alessandri Grant/research support from: Pfizer, Guido Valesini: None declared, Fabrizio Conti Speakers bureau: BMS, Lilly, Abbvie, Pfizer, Sanofi

scholarly journals Evaluation of Gingival Microleakage in Class II Composite Restorations with Different Lining Techniques: An In Vitro Study

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Vedavathi Bore Gowda ◽  
B. V. Sreenivasa Murthy ◽  
Swaroop Hegde ◽  
Swapna Devarasanahalli Venkataramanaswamy ◽  
Veena Suresh Pai ◽  
...  
Keyword(s):  
Study Groups ◽  
Class Ii ◽  
Composite Liner ◽  
Flowable Composite ◽  
Composite Restorations ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. To compare the microleakage in class II composite restorations without a liner/with resin modified glass ionomer and flowable composite liner.Method. Forty standardized MO cavities were prepared on human permanent mandibular molars extracted for periodontal reasons and then divided into 4 groups of ten specimens. The cavity preparations were etched, rinsed, blot dried, and light cured and Adper Single Bond 2 is applied. Group 1 is restored with Filtek P60 packable composite in 2 mm oblique increments. Group 2 is precure group where 1 mm Filtek Z350 flowable liner is applied and light cured for 20 sec. Group 3 is the same as Group 2, but the liner was cocured with packable composite. In Group 4, 1 mm RMGIC, Fuji Lining LC is applied and cured for 20 sec. All the teeth were restored as in Group 1. The specimens were coated with nail varnish leaving 1 mm around the restoration, subjected to thermocycling, basic fuchsin dye penetration, sectioned mesiodistally, and observed under a stereomicroscope.Results. The mean leakage scores of the individual study groups were Group 1 (33.40), Group 2 (7.85), Group 3 (16.40), and Group 4 (24.35). Group 1 without a liner showed maximum leakage. Flowable composite liner precured was the best.

scholarly journals Effectiveness of a silicon-based root canal sealer for filling of simulated lateral canals

2007 ◽  
Vol 18 (1) ◽  
pp. 20-23 ◽  
Author(s):  
João Vicente Baroni Barbizam ◽  
Matheus Souza ◽  
Doglas Cecchin ◽  
Jakob Dabbel
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Root Canal Sealer ◽  
Human Teeth ◽  
Significance Level ◽  
Working Length ◽  
Group 3 ◽  
Group 2 ◽  
Silicon Based

The purpose of this in vitro study was to evaluate the ability of a silicon-based root canal sealer, compared to zinc oxide and eugenol and an epoxy resin-based sealers, for filling of simulated lateral canals. Thirty extracted single-rooted human teeth were selected, conventional access was made and the working length was established 1 mm from the apical foramen. Three simulated lateral canals, one in each root third (coronal, middle and apical) were prepared in both the mesial and distal surfaces of each tooth using a size 15 reamer adapted to a low-speed handpiece. Each root canal was instrumented using ProTaper rotary files up to file F3 at the working length, and then irrigated with 2.5% NaOCl followed by EDTA. The teeth were assigned to 3 groups (n=10), according to the root canal sealer: Roeko Seal (Group 1), Sealer 26 (group 2) and Grossman's sealer (Group 3). Gutta-percha cold lateral condensation technique was performed in all groups. Postoperative radiographs were taken and the images were projected for evaluation of the quality of lateral canal filling. Data were submitted to statistical analysis by Kruskal Wallis test at 5% significance level. The results showed that Grossman's sealer filled a larger number of lateral canals than Roeko Seal (p<0.05) and Sealer 26 (p<0.01). It may be concluded that Roeko Seal silicone-based root canal sealer was not as effective as the Grossman's sealer for filling of simulated lateral canals. The lateral canals localized in the apical third of the root were more difficult to be filled.

scholarly journals Experimental Reproduction of Bovine Fetal Neospora Infection and Death with a Bovine Neospora Isolate

1994 ◽  
Vol 6 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Bradd C. Barr ◽  
Joan D. Rowe ◽  
Karen W. Sverlow ◽  
Robert H. BonDurant ◽  
Alex A. Ardans ◽  
...  
Keyword(s):  
Uninfected Cell ◽  
In Utero ◽  
Pathogenic Potential ◽  
Group 4 ◽  
Fetal Infection ◽  
Group 3 ◽  
Group 2 ◽  
Protozoal Infection ◽  
Group 1

Studies were conducted to determine the pathogenic potential of the recently isolated bovine Neospora protozoa (BPA-1) for the bovine fetus. Cows chosen for study had Neospora titers < 160 using an indirect immunofluorescent antibody (IFA) test. Four experimental groups were studied. In group 1, 2 fetuses were inoculated in utero at 118 days gestation with culture-derived Neospora tachyzoites. A pregnant control cow was housed in the same pen, observed daily and screened serologically for evidence of exposure to Neospora. In group 2, 2 cows were infected with Neospora tachyzoites at 138 or 161 days gestation, and 1 control cow was given uninfected cell culture suspension simultaneously at 154 days gestation. Groups 3 (85 days gestation) and 4 (120 days gestation) each consisted of 2 cows infected with Neospora tachyzoites and 1 control cow given uninfected material at the same stage of gestation. Dead fetuses were surgically removed from the infected cows in group 1 on postinfection day (PID) 17. The histopathology was compatible with protozoal fetal infection, and protozoa were identified by immunohistochemistry. Viable fetuses were removed surgically from cows in group 2 on PID 28-30. The histopathology was compatible with protozoal fetal infection, protozoa were identified by immunoperoxidase techniques, and Neospora tachyzoites were reisolated in vitro from tissues of the 2 infected fetuses. In groups 3 and 4, the control fetus and 1 infected fetus were removed surgically between PID 26 and PID 33. The remaining infected cows were observed until fetal death or abortion occurred. In group 3, the fetus that was surgically removed from 1 infected cow had no pathologic abnormalities, and parasites were not found (PID 26). The second fetus in group 3 died in utero, and expulsion of a mummified fetus was induced on PID 67. Brain histopathology was compatible with protozoal infection, and parasites were identified by immunoperoxidase techniques. The fetus that was surgically removed (PID 32) from 1 infected cow in group 4 had lesions compatible with protozoal infection, and Neospora tachyzoites were reisolated in vitro from fetal tissues. The second infected cow in group 4 produced a full-term live calf that had a precolostral Neospora titer of 20,480. Clinically, this calf had depressed conscious proprioception in all limbs. Very mild lesions were found in the central nervous system, but protozoa were not found in the tissues. The results demonstrate that the bovine Neospora protozoa can be transplacentally transmitted, resulting in fetal infection and death, and mimics the naturally occurring fetal disease.

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