Occlusion Effect of Dentinal Tubules of the Dentifrice Containing Sodium Metasilicate

2007 ◽  
Vol 330-332 ◽  
pp. 1429-1432
Author(s):  
Su Young Lee ◽  
S.O. Jang ◽  
Hae Sun Kim ◽  
Jai Hyuk Choi ◽  
H.K. Kwon ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Metasilicate ◽  
Control Group ◽  
Strontium Chloride ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
The Difference ◽  
Silicone Carbide ◽  
Occlusion Effect

Exposed dentine with patent tubules allows the movement of tubule fluid leading to dentine sensitivity. Occlusion of patent dentinal tubules effectively reduces the state of dentine hypersensitivity. Strontium chloride (SrCl2) has been well known as a component of desensitizing dentifrice. Recently, new dentifrice containing sodium metasilicate was on the market for desensitizing dentifrice. The aim of this in vitro study was to compare the occlusion effect of dentinal tubule between the sodium metasilicate and the existing desensitizing dentifrices. Sixtyfour human dentine specimens were embedded into a Teflon mold and were grinded with the use of silicone carbide papers to expose the surface of dentin. The dentin surface was etched with 37% phosphoric acid for 60 seconds and was treated with ultrasonic washing for 1 hour. The dentifrices used in the study are classified into four groups: G1-dentifrice containing Sodium metasilicate, G2- dentifrice containing Strontium chloride, G3-dentifrice containing Hydroxyapatite and G4-BSI reference dentifrice (control group). Toothpaste slurries were prepared as 20 gram of toothpaste in 80 ml of distilled water. Thirty-two specimens were brushed with the toothpaste slurries using V8 Cross Brushing Machine. Tooth-brushing was performed 5,000 times using a back-and–forth stroke. The pictures of the sizes of dentinal tubules were taken by scanning electron microscopy (×3000) and changes of tubule sizes were analyzed by digital analysis. The results showed that the difference of tubule size in descending order: G1-3.30±1.80, G2-2.82±1.73, G3-1.49±1.14, G4- 1.04±0.94. The dentifrice containing Sodium metasilicate (22.5%) showed the highest dentinal tubule occlusion. Moreover, the dentifrice containing Sodium metasilicate statistically significantly increased occlusion of dentinal tubule as compared to BSI reference dentifrice (p<0.05). Thus, new desensitizing dentifrice containing sodium metasilicate was more effective than existing desensitizing dentifrice on occlusion of dentin tubules.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

scholarly journals “COMPARATIVE EVALUATION OF EFFECT ON DENTINAL TUBULE OCCLUSION BY THREE DIFFERENT DESENSITIZING DENTIFICES: A SEM STUDY”

2021 ◽  
Vol 5 (11) ◽  
Author(s):  
Aman Mallick ◽  
Gaurav Malhotra ◽  
Pradeep Shukla ◽  
Prerna Kataria ◽  
C. S Joshi
Keyword(s):  
Strontium Chloride ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentine Hypersensitivity ◽  
Study Results ◽  
Sem Study ◽  
Group 3 ◽  
Group 2 ◽  
Tubule Occlusion

Introduction: Dentine hypersensitivity (DH) occurs on exposed dentine and is dependent on the patency of dentinal tubules. This study compared the effectiveness of toothpaste containing Strontium Chloride, Novamine, Pro-Arginine in occluding dentine tubules along with comparision of those. Materials and Method: 45 freshly extracted teeth were randomly divided (15 each) into 3 groups: Group 1 15 Teeth treated with toothpaste containing Strontium Chloride. Group 2 15 Teeth treated with toothpaste containing Novamine Group 3 15 Teeth treated with toothpaste containing Pro-Arginine. Each specimen was brushed with the dentifrice slurries and examined under SCM. Result: The mean of occluded dentinal tubules on the dentin surface after brushing with three dentifrices were significant among them Pro-arginine group showed maximum occlusion. Conclusion: The present in vitro SEM study results revealed that:  All the experimental agents– Strontium chloride, novamin, Pro- Argenine were effective in occluding dentinal tubules & the percentage (%) of occluded tubules was found to be highest for Pro- Argenine as compared to the other groups. Keywords: SCM, DH

scholarly journals Dissolution capacity of orange and eucalyptus oils versus endodontic types of cement (in vitro study)

2022 ◽  
Vol 10 (19) ◽  
pp. 29-33
Author(s):  
Sebastián M. Ulloa-Alvarez ◽  
María E. Guerrero-Coello ◽  
José E. Torracchi-Carrasco
Keyword(s):  
In Vitro Study ◽  
Control Group ◽  
Filling Material ◽  
Cement Sample ◽  
Orange Oil ◽  
Eucalyptus Oil ◽  
Before And After ◽  
The Difference ◽  
Post Hoc

Introduction: Endodontic retreatment seeks reentry to the root canal system to remove all filling material, identification and correction of pathological or iatrogenic defects. Objective: This study aimed to compare the dissolving capacity of eucalyptus oil, orange oil, and distilled water versus three types of endodontic cements. Materials and methods: Two hundred and four stainless steel molds were fabricated, where each cement sample was placed to the edge and left to set in the incubator, simulating normal oral conditions for 7 days. Subsequently, the solubility of each cement was analysed by immersing the samples in eucalyptus and orange oil for ten minutes in a static and ultrasonic environment. The solubility of the cement was evidenced by the difference between the weight before and after contact with the oils, data were statistically analysed with the ANOVA test and the post hoc Tukey bootstrap means test with 1,000 repetitions. Results: The control group and eucalyptus oil having no significant effect (p>0.05), the ultrasonic environment was found to be effective (p<0.05), whereas orange oil had better dissolution effects (p=0.004). Conclusions: The most susceptible cement was Sealapex, while the least soluble was MTA-Fillapex in all the solvents studied.

scholarly journals Fibroblast Proliferation due to Exposure to a Platelet Concentrate: An in vitro Study

2010 ◽  
Vol 1 (3) ◽  
pp. 163-166
Author(s):  
Shelly Ahuja
Keyword(s):  
Growth Factors ◽  
Third Molar ◽  
Test Group ◽  
In Vitro Study ◽  
Platelet Concentrate ◽  
Control Group ◽  
Cellular Events ◽  
The Difference ◽  
And Control

ABSTRACT Introduction The major cellular events in the tissue repair are mitogenesis, migration and metabolism. The proteins responsible for coordination of these events are called “growth factors”. The activated platelets at the wound margins release several growth factors, such as PDGF, TGF-β and EGF, etc., and plasma exudates also provide an important source of TGF-β factors. Materials and methods Periodontal ligament fibroblast obtained from third molar impaction surgery, periodontal ligaments were cultured under standard conditions and spread on 96 well tissue culture plates. Platelet concentrate was obtained after centrifugation of 350-400 ml of blood at 1000 and 5000 rpm. 15 μl of platelet concentrate was added to each well. The proliferation rate of test and control group was determined by Redox indicator (Alamar blue® assay). The number of cells were counted by neu bar counting chamber after 24, 48 and 72 hours. Results The proliferation activity of cells was considerably higher in the platelet concentrate group (test group) than the control group. The difference was highly significant upto 72 hours after addition of platelet concentrates (Mann-Whitney U test p < 0.001). Conclusion A cellular effect of the platelet concentrate is clearly discernible. It was concluded that the use of platelet concentrate is an effective modality of regeneration.

Occlusion Effect of Dentinal Tubules of the Dentifrice Containing Nano-Sized Carbonate Apatite

2007 ◽  
Vol 342-343 ◽  
pp. 821-824
Author(s):  
Su Young Lee ◽  
Sun Ok Jang ◽  
H.K. Kwon ◽  
Young Sik Cho ◽  
B.I. Kim
Keyword(s):  
Strontium Chloride ◽  
Carbonate Apatite ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentin Surface ◽  
Carbonated Apatite ◽  
Group 4 ◽  
Significant Difference ◽  
Group 1

Exposed dentine with patent tubules allows the movement of the tubule fluid leading to dentine sensitivity. An occlusion of patent dentinal tubules can effectively reduce the state of dentine hypersensitivity. Strontium chloride (SrCl2) has been known as a representative component of a desensitizing dentifrice. Recently, a desensitizing dentifrice containing nano-sized Carbonated Apatites (n-CAPs) was released onto the market. The aim of this in vitro study was to evaluate the occlusion effects of dentinal tubules in dentifrices containing several concentrations of the n-CAPs. One hundred human dentine specimens were embedded into a Teflon mold and ground with silicone carbide papers to expose the dentin surface. The dentin surface was etched with 37% phosphoric acid for 60 seconds and washed ultrasonically for 1 hour. The dentifrices were classified into five groups: experimental dentifrices Group 1: n-CAPs 5%, group 2: n-CAPs 15%, group 3: n- CAPs 0% and group 4: n-CAPs 30%, and the control dentifrice group 5: SrCl2 10%. The dentifrice slurries were prepared as 20 grams of dentifrice in 80 ml of distilled water. Fifty specimens were brushed with the dentifrice slurries using a V8 Cross Brushing Machine. Tooth brushing was performed 5,000 times using a back-and–forth stroke. Images showing the sizes of the dentinal tubules were taken by scanning electron microscopy (×3000) and the changes in the tubule sizes were analyzed using a digital analyzer. The n-CAPs used in this study had a high solubility and affinity to bone minerals. In addition, the n-CAPs were well deposited on patent dentinal tubules. The group 1 dentifrice (n-CAPs 5% and silica 25%) showed the highest dentinal tubule occlusion, and there was a statistically significant difference between group 4 and the control (p<0.05). Overall, the dentifrice containing 5% nano carbonated apatite and 25% silica is the most effective in occluding dentinal tubules.

scholarly journals Occluding Effect of Nd:YAG Laser and Different Dentin Desensitizing Agents on Human Dentinal Tubules In Vitro: A Scanning Electron Microscopy Investigation

2012 ◽  
Vol 37 (4) ◽  
pp. 340-355 ◽  
Author(s):  
LMS Al-Saud ◽  
HNA Al-Nahedh
Keyword(s):  
Nd:Yag Laser ◽  
Ethylenediaminetetraacetic Acid ◽  
In Vitro Study ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentin Surface ◽  
Desensitizing Agents ◽  
The One ◽  
Scanning Electron

SUMMARY Objectives This in vitro study aimed to microscopically evaluate and compare the occluding effect of the Nd:YAG laser and different dentin desensitizing agents on human dentinal tubules. Materials and Methods The Nd:YAG laser (SunLase™ 800) and four commercially available and professionally applied dentin desensitizers (Gluma® desensitizer, Tenure Quick®, Quell™ desensitizer, and VivaSens®) were investigated in this study. Sixty-four extracted intact human molars were used. Each dentin surface was divided by shallow indentation into two halves, one of which was used for treatment and the other of which served as a control. The dentin surfaces were etched to remove any smear plugs and to mimic the open dentinal tubules of sensitive dentin using 0.5 M ethylenediaminetetraacetic acid (pH 7.4) for two minutes (applied with a microbrush) and then rinsed with an air-water syringe for 30 seconds. The laser samples (n=16) were randomly divided into four groups of four samples each. These groups were the step-up technique group, the 14-day group, the one-minute group, and the two-minute group. Forty-eight samples were treated with the four tested desensitizing agents and were randomly divided into four groups (n=12/group). Each group was further subdivided into three subgroups (n=4). Samples of the first subgroup were treated for 14 days, while those of the second subgroup were treated once. Samples of the last subgroup were fractured longitudinally after a single treatment. All of the samples were then examined under a scanning electron microscope. Results The Nd:YAG laser–irradiated dentin showed reduction or complete obliteration of the dentinal tubule lumen; thus, the treatment modified the original dentinal structure. The lased dentin surface in the two-minute group showed bubble-like changes in the area of the dentinal tubules' orifices. Statistically, the two-minute group was found to have a significantly higher percentage of partially or fully occluded tubules than did the one-minute group. All of the studied desensitizing agents produced occlusion of the dentinal tubules; however, the appearance of the precipitates, the level of coverage, and the degree of dentinal occlusion varied among the tested products. Conclusion Throughout the specified period of this study, occlusion and/or narrowing of the open dentinal tubules have been successfully achieved with both treatment approaches.

scholarly journals The influence of vinegars on exposure of dentinal tubules: a SEM evaluation

2004 ◽  
Vol 18 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Daniela Leal Zandim ◽  
Fernanda Oliveira Bello Corrêa ◽  
José Eduardo Cezar Sampaio ◽  
Carlos Rossa Júnior
Keyword(s):  
Dietary Habits ◽  
In Vitro Study ◽  
Smear Layer ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Human Teeth ◽  
Painful Condition ◽  
Dentinal Tubules ◽  
Significant Difference

Dentin hypersensitivity is a common painful condition observed in clinics. Dietary habits have been much associated with its development and persistence during and following periodontal treatment. The aim of this in vitro study was to evaluate the influence of vinegars on the removal of smear layer and exposure of dentinal tubules. Extracted human teeth were submitted to manual scaling with Gracey curettes in order to remove the cementum as well as to form a smear layer. Dentin samples with 3 mm² were obtained and distributed into six experimental groups: one control and five types of vinegars (alcohol, apple, rice, white wine and balsamic). Each group included two methods of vinegar application: topical and friction. After routine preparation for SEM analysis, photomicrographs were assessed by a calibrated and blind examiner using an appropriate index system. Kruskal-Wallis test indicated a significant influence of vinegars on smear layer removal. There was a statistically significant difference between groups treated with apple, white and rice vinegars and the control group (p < 0.05). Nevertheless, Mann-Whitney test indicated that removal of smear layer did not vary with the method of application (topical versus friction) for any of the tested substances. We can conclude that the contact of vinegar may remove smear layer and expose dentinal tubules, regardless of the type of application. However, balsamic vinegar was associated with less removal of smear layer after both methods of application.

scholarly journals Comparison of Opening Torque of Grooved and Non-grooved Memory Alloy Screws for Mandibular Fracture Fixation

2014 ◽  
Vol 6 (2) ◽  
pp. 54-59
Author(s):  
Bijan Movahhedian ◽  
Shadab Ghods ◽  
Anahita Azadi ◽  
Mahdi Kadkhodazadeh
Keyword(s):  
Shape Memory ◽  
Fracture Fixation ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Mandibular Fracture ◽  
Control Group ◽  
Mandibular Fractures ◽  
The Mean ◽  
The Difference

Background and aims. The aim of this study was to assess the opening torque of grooved and non-grooved screws made of shape memory alloys (SMA) for fixation of mandibular fractures. Materials and methods. In this in vitro study the opening torques of ten SMA screws with grooves and ten SMA screws without grooves were compared in 20 holes (10 holes for grooved screws and 10 holes for non-grooved screws) placed in the bovine mandible. Statistical significance was set at P < 0.05. Results. The mean opening torque was 2.27 ± 0.43 nm for screws in the control group and 2.05 ± 0.45 nm for screws in the grooved group (P < 0.05, P = 0.08). Conclusion. This study revealed that the torque needed for opening the grooved screws was higher in comparison with current fixation screws; however, the difference was not statistically significant.

scholarly journals Biomimetic Mechanism of Action of Fluoridated Toothpaste Containing Proprietary REFIX Technology on the Remineralization and Repair of Demineralized Dental Tissues: An in vitro Study

2020 ◽  
Author(s):  
Fabiano Vieira Vilhena ◽  
Sonia Mariza Luiz de Oliveira ◽  
Marcelo Henrick Maia Matochek ◽  
Pedro Luiz Santos Tomaz ◽  
Thales de Sá Oliveira ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Elemental Analysis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Cross Sectional ◽  
Dentinal Tubules ◽  
Dental Tissues ◽  
Ph Cycling

Abstract Objectives This in vitro study aimed to characterize the mineral content and surface and cross-sectional morphology of enamel and dentin tissues treated with a 1450 ppm fluoride-containing toothpaste with REFIX technology. Materials and Methods Bovine enamel blocks (n = 5) were obtained (4 × 4 × 6 mm), demineralized (artificial caries lesion), and treated (pH cycling and brushing with the toothpaste). During the pH cycling, which lasted for 7 days (demineralization and remineralization took 6 and 18 hours, respectively), the enamel was brushed for 5 minutes using an electric toothbrush before being immersed in a remineralizing solution. The dentin blocks were acid-etched for 2 minutes (0.05 M citric acid, pH 1.8) to expose the dentinal tubules (n = 5). Morphological analysis of the dentin was performed immediately and after 7 days of brushing with the dentifrice, and compared with the control group. The specimens were then cross-sectioned. The surface and cross-sectional micromorphology were assessed using scanning electron microscopy (SEM). The elemental analyses (weight%) were determined with an energy-dispersive X-ray spectroscopy (EDS). Results The toothpaste with REFIX technology remineralized and repaired the surface enamel effectively. The elemental analysis also demonstrated that treating the enamel with the toothpaste formed a silicon-enriched mineral layer on the enamel surface. Elemental analysis of the enamel cross-sections showed that the toothpaste induced a mineral change. The results were also consistent in the dentin, where the dentinal tubules were progressively occluded until there was complete occlusion after 7 days. Conclusions We prove the biomimetic mechanism of action of fluoridated toothpaste containing proprietary REFIX technology for obtaining silicon-enriched, remineralized and repaired dental tissues.

Evaluation of the Effects of Different Composite Types on the Shear Bond Strength: An in vitro Study

2021 ◽  
Author(s):  
Mahdi Shahrabi ◽  
Behnam Seraj ◽  
Shabnam Milani ◽  
Seyde Fateme Rezaei Taleshi ◽  
Sara Yaghoubirad ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Case Group ◽  
Student Test ◽  
The Difference ◽  
Cylindrical Samples

Introduction: The aim of this in vitro study was to determine and compare the shear bond strength (SBS) of two types of composites. Material and Methods: Twenty cylindrical samples were prepared and divided into two control and case groups. The case group consisted of 10 cylindrical samples of two types of composites (3M and Vivadent), and the control group, which was divided into two subgroups of 5, included only one type of composite (3M or Vivadent). After preparation, the samples were transferred to the Instron device for testing the SBS. The data were analyzed by SPSS version 15 software using Smirnov-Kolmogorov test for studying the normal distribution of data and t-student test for independent populations. Results: Heliomolar (Ivoclar-Vivadent) composite (microfill) showed the lowest SBS, but the difference was not significant (P>0.05). The highest SBS was for Filtek Z100 (3M ESPE) composite. There were no fracture lines in the interface of the two composites. Conclusion: This confirms that proper use of adhesives can prevent fracture in the interface of different composites. So, using two different composite brands on each other does not reduce the bond strength between them.

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