Antibacterial Effect of Platelet Concentrate and Amniotic Membrane as two Human-derived Biological Products

2021 ◽  
Vol 20 (3) ◽  
pp. 262-271
Author(s):  
Shahrzad Someh Sarai Sabet ◽  
◽  
Teena Dadgar ◽  
Hadi Bazzazi ◽  
◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Bacterial Resistance ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Blood Platelet ◽  
Multidrug Resistant ◽  
Platelet Concentrate ◽  
Biochemical Tests ◽  
Methicillin Resistant ◽  
Biological Products

Background and Objectives: Regarding the increasing spread of bacterial resistance, researchers are always interested in finding effective antibiotics of natural origin. The amniotic membrane and blood platelet concentrate are two biological products with an antibacterial effect. The present study aimed to investigate the antibacterial effect of the biological products on broad-spectrum MBL-producing Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. Subjects and Methods The amniotic membrane, blood platelet concentrate, S. aureus, and P. aeruginosa isolates were collected from hospitals in Gorgan City, Iran. The isolates were identified using the biochemical tests. The methicillin-resistant S. aureus and MBL-producing P. aeruginosa strains were collected by the combined disk and iodometric methods. The antibacterial effects of platelet serial dilutions of bacteria were prepared using 0.5 McFarland turbidity standard suspension in tubes. Then, different concentrations of bacteria were mixed with platelet. After four different encounter durations, a sample was obtained and cultured on medium and bacterial growth was examined. The amniotic membrane was assessed by disk diffusion methods. Results The results showed that all isolates of P. aeruginosa and S. aureus were MBL producers. The platelet concentrate showed the antibacterial effect on all S. aureus isolates, whereas it lacked such an effect on P. aeruginosa isolates. It indicates that the amniotic membrane has an antibacterial effect on all S. aureus and P. aeruginosa isolates. Conclusion The amniotic membrane and platelet concentrate showed high antimicrobial potential against multidrug-resistant S. aureus and P. aeruginosa pathogens. Therefore, human-derived natural products can be used as a source for efficient antibiotics.

scholarly journals The Prevalence of Virulence Determinants and Antibiotic Resistance Patterns in Methicillin—Resistant Staphylococcus aureus in a Nursing Home in Poland

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 427
Author(s):  
Martyna Kasela ◽  
Agnieszka Grzegorczyk ◽  
Bożena Nowakowicz-Dębek ◽  
Anna Malm
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Variable Number Tandem Repeat ◽  
Multidrug Resistant ◽  
Variable Number ◽  
Pulse Field ◽  
Virulence Determinants ◽  
Gene Encoding ◽  
Methicillin Resistant ◽  
Pulse Field Gel Electrophoresis

Nursing homes (NH) contribute to the regional spread of methicillin-resistant Staphylococcus aureus (MRSA). Moreover, residents are vulnerable to the colonization and subsequent infection of MRSA etiology. We aimed at investigating the molecular and phenotypic characteristics of 21 MRSA collected from the residents and personnel in an NH (Lublin, Poland) during 2018. All MRSA were screened for 20 genes encoding virulence determinants (sea-see, eta, etb, tst, lukS-F-PV, eno, cna, ebpS, fib, bbp, fnbA, fnbB, icaADBC) and for resistance to 18 antimicrobials. To establish the relatedness and clonal complexes of MRSA in NH we applied multiple-locus variable-number tandem-repeat fingerprinting (MLVF), pulse field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. We identified four sequence types (ST) among two clonal complexes (CC): ST (CC22) known as EMRSA-15 as well as three novel STs—ST6295 (CC8), ST6293 (CC8) and ST6294. All tested MRSA were negative for sec, eta, etb, lukS-F-PV, bbp and ebpS genes. The most prevalent gene encoding toxin was sed (52.4%; n = 11/21), and adhesins were eno and fnbA (100%). Only 9.5% (n = 2/21) of MRSA were classified as multidrug-resistant. The emergence of novel MRSA with a unique virulence and the presence of epidemic clone EMRSA-15 creates challenges for controlling the spread of MRSA in NH.

Non-Multidrug-Resistant, Methicillin-Resistant Staphylococcus aureus in a Neonatal Unit

2014 ◽  
Vol 33 (10) ◽  
pp. e252-e259 ◽  
Author(s):  
Cilmara P. Garcia ◽  
Juliana F. Rosa ◽  
Maria A. Cursino ◽  
Renata D. Lobo ◽  
Carla H. Mollaco ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Neonatal Unit ◽  
Methicillin Resistant

scholarly journals Antibiotic Susceptibility Pattern of Nosocomial Isolates of Staphylococcus aureus in a Tertiary Care Hospital, Nepal

2009 ◽  
Vol 48 (175) ◽  
Author(s):  
Bidya Shrestha ◽  
B M Pokhrel ◽  
T M Mohapatra
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tertiary Care ◽  
Test Method ◽  
University Teaching ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Care Hospital ◽  
Biochemical Tests ◽  
Methicillin Resistant

Introduction: Methicillin resistant Staphylococcus aureus (MRSA), the most common cause ofnosocomial infection has been a major cause of morbidity and mortality around the world. They arenormally resistant to most of the antibiotics used in clinical practice. This study has been carried outto fi nd out the resistance pattern among S. aureus.Methods: During November 2007 to June 2008, clinical samples from patients with nosocomialinfection were processed for culture and sensitivity following standard methodology in microbiologylaboratory, Tribhuvan University teaching hospital, Kathmandu, Nepal.Results: Among 149 Staphylococcus aureus isolates, highest resistance was observed against Penicillin(91.94%) followed by Fluoroquinolone (61.74%), Erythromycin (52.94%), Gentamicin (46.98%),Cotrimoxazole (42.95%), Tetracycline (40.94%) and others, whereas susceptibility was observedmaximum against Chloramphenicol (94.85%) followed by Rifampicin (92.61%), Tetracycline(59.06%), Cotrimoxazole (57.04%), and others. None of the isolates were resistant to Vancomycinand Teicoplanin. Of these isolates 44.96 % of the isolates were Methicillin resistant S. aureus (MRSA).Resistance to Penicillin, Fluoroquinolone, Erythromycin, Gentamicin, Co-trimoxazole and Tetracyclinewere associated signifi cantly with MRSA isolates (X2= 8.779, p<0.05, X2= 74.233, p<0.05, X2= 84.2842,p<0.05, X2= 108.2032, p<0.05, X2= 88.1512, p<0.05 and X2= 79.1876, p<0.05 respectively). Althoughmost of the Methicillin sensitive S. aureus (MSSA) isolates were susceptible to both Rifampicinand Chloramphenicol, only Rifampicin susceptibility was signifi cantly associated with them (X2=10.1299, p<0.05). Among three Biochemical tests for the detection of β lactamase detection namelychromogenic, iodometric and acidimetric test, chromogenic test method had highest sensitivity andspecifi city.Conclusions: Since MRSA comprised a greater part of S. aureus isolates and were multi-resistant,patients infected by such strains should be identifi ed and kept in isolation for hospital infectioncontrol and treated with second line of drug like vancomycin.Key Words: β lactamase, methicillin resistant Staphylococcus aureus,methicillin sensitive Staphylococcus aureus, resistance pattern

scholarly journals IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS USING TOUCHDOWN PCR AND PHENOTYPIC METHODS FROM PATIENTS AND HOSPITALS ENVIRONMENTS IN DIFFERENT IRAQI CITIES

2021 ◽  
Vol 52 (6) ◽  
pp. 1356-1364
Author(s):  
A. M. Abd Zaid ◽  
N. J. Kandala
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Multidrug Resistant ◽  
Sensitivity Test ◽  
Methicillin Resistant ◽  
Cultural Methods ◽  
Base Medium ◽  
Touchdown Pcr ◽  
Phenotypic Methods

The study was aimed to evaluate the prevalence of MRSA in some Iraqi hospitals and determine the most powerful methods for identification of MRSA, in order to achieve the, 278 samples were collected from different hospitals in Iraq in various intervals, 204 out of 287 were identified as Staphylococcus aureus by conventional cultural methods and microscopic characteristics and 177 isolates are identified as MRSA by using HiCrome MeReSa Agar Base medium, but 154 of 177 (87%) isolates are methicillin resistance in sensitivity test. MRSA isolates were highly resistant to β-lactam antibiotics and considered multidrug resistant (MDR) in percent of (94.9%). Touchdown PCR used to identify the isolates, 97.05% were identified as Staphylococcus aureus, while 80.88%  as MRSA.                  

scholarly journals Prevalence of Methicillin-Resistant Staphylococcus aureus on Paper Currency Notes

2021 ◽  
pp. 25-32
Author(s):  
Rikhi Ram Marasini ◽  
Pratikshya Shrestha ◽  
Prabhat Dhakal ◽  
Sukra Raj Shrestha ◽  
Sirjana Adhikari ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Brain Heart Infusion ◽  
Biochemical Tests ◽  
Mannitol Salt Agar ◽  
Methicillin Resistant ◽  
Total Prevalence ◽  
Paper Currency ◽  
Antibiotic Susceptibility Test ◽  
Hygiene Measures

The main objective of this study was to determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) in paper currency. The paper currencies in circulation in Pokhara Metropolitan City were inspected. Bills of various denominations (Rs 5, 10, 20, 50, 100, 500 and 1000) were collected from five different locations; namely Food and Vegetable Shop, Bus conductor, Hospital Pharmacy, Butcher Shop and Grocery Shop. Collected sample were cultured and incubated for 24 hours at 37 oC in Brain Heart Infusion (BHI) Broth. The inoculums were further cultured on Mannitol Salt Agar (MSA) and Blood Agar (BA) media to obtain colonies, which were examined and evaluated for various parameters like gram staining and biochemical tests for identification. Then, antibiotic susceptibility test of the isolates was performed using standard procedures. A total of 35 sample of paper currency were processed, all of which showed positive growth. Out of 86 total isolates, 21 (24.42%) were Staphylococcus aureus followed by Coagulase Negative Staphylococci 19 (22.09%), Diptheroids 14 (16.3%), Bacillus spp 13 (15.11%), Micrococci 9 (10.46%), Streptococcus pneumonia 4 (4.65%), Viridans Streptococcus 4 (4.65%) and Streptococcus pyogenes 2 (2.32%). The total prevalence of MRSA in this study was 7 (33.33%). Paper currency contaminated with MRSA poses a high threat to those handling the bills as well as the community. Thus, this study suggests proper hygiene measures to be adopted after handling of paper currency to minimize the risk of contamination and emergence of diseases.

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