The Differential Anti-HIV Effect of a New Humic Substance-Derived Preparation in Diverse Cells of the Immune System

The anti-HIV activity of a new humic substance-derived preparation has been studied in individual pools of immune cells (CD4+ T lymphocytes, macrophages, dendritic cells). Near-complete inhibition of the HIV infection (by more than 90%) was achieved by treating each of the abovementioned cell types with non-toxic concentrations of the preparation. The inhibitory effect demonstrates the possibility of preventing the depletion of a significant portion of functionally important immune cells. A comparative study of infection inhibition in individual cell pools has allowed us to reveal the differences in the preparations effectiveness in each of the cell populations. A R5-tropic HIV-1 infection in macrophages exhibited maximum sensitivity to the preparation: 90% and 50% inhibition of the infection were observed in the presence of concentrations as low as 1.4 and 0.35 g/ml, respectively. A 15- and 19-fold higher concentration was required to achieve the same extent of inhibition in dendritic cells infected with the same strain. The effectiveness of the drug in CD4 + T lymphocytes is quite comparable to its effectiveness in macrophages. The drug is universally effective for both the T- and M-tropic variants of HIV-1.

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Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells

Dendritic Cell Protocols ◽

10.1385/1-59259-150-7:441 ◽

2003 ◽

pp. 441-453

Author(s):

Xiaoli Huang ◽

Zheng Fan ◽

Lian Zheng ◽

Charles R. Rinaldo

Keyword(s):

Dendritic Cells ◽

T Lymphocytes ◽

Cytotoxic T Lymphocytes ◽

Anti Hiv ◽

Hiv 1 ◽

Stimulation Of

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Plasmacytoid Dendritic Cells Impair Anti-HIV Immunity and HIV-1 Persistence During Antiretroviral Therapy Via IDO-Dependent Mechanisms

SSRN Electronic Journal ◽

10.2139/ssrn.3471314 ◽

2019 ◽

Author(s):

Guangming Li ◽

Jianping Ma ◽

Haisheng Yu ◽

Wenwen Bi ◽

Xuguang Zhai ◽

...

Keyword(s):

Dendritic Cells ◽

Antiretroviral Therapy ◽

Plasmacytoid Dendritic Cells ◽

Anti Hiv ◽

Hiv 1

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Nitric Oxide Regulates MIP-1α Expression in Primary Macrophages and T Lymphocytes: Implications for Anti-HIV-1 Response

Molecular Medicine ◽

10.1007/bf03401793 ◽

2000 ◽

Vol 6 (6) ◽

pp. 542-549 ◽

Cited By ~ 10

Author(s):

Barbara Sherry ◽

Helena Schmidtmayerova ◽

Gabriele Zybarth ◽

Larisa Dubrovsky ◽

Tobias Raabe ◽

...

Keyword(s):

Nitric Oxide ◽

T Lymphocytes ◽

Anti Hiv ◽

Hiv 1

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Vpu Exploits the Cross-Talk between BST2 and the ILT7 Receptor to Suppress Anti-HIV-1 Responses by Plasmacytoid Dendritic Cells

PLoS Pathogens ◽

10.1371/journal.ppat.1005024 ◽

2015 ◽

Vol 11 (7) ◽

pp. e1005024 ◽

Cited By ~ 29

Author(s):

Mariana G. Bego ◽

Édouard Côté ◽

Nick Aschman ◽

Johanne Mercier ◽

Winfried Weissenhorn ◽

...

Keyword(s):

Dendritic Cells ◽

Cross Talk ◽

Plasmacytoid Dendritic Cells ◽

The Cross ◽

Anti Hiv ◽

Hiv 1

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Heterologous Human Immunodeficiency Virus Type 1 Lentiviral Vectors Packaging a Simian Immunodeficiency Virus-Derived Genome Display a Specific Postentry Transduction Defect in Dendritic Cells

Journal of Virology ◽

10.1128/jvi.77.17.9295-9304.2003 ◽

2003 ◽

Vol 77 (17) ◽

pp. 9295-9304 ◽

Cited By ~ 31

Author(s):

Caroline Goujon ◽

Loraine Jarrosson-Wuilleme ◽

Jeanine Bernaud ◽

Dominique Rigal ◽

Jean-Luc Darlix ◽

...

Keyword(s):

Gene Therapy ◽

Human Immunodeficiency Virus ◽

Dendritic Cells ◽

Simian Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Cell Types ◽

Immunodeficiency Virus ◽

Hiv 1

ABSTRACT Heterologous lentiviral vectors (LVs) represent a way to address safety concerns in the field of gene therapy by decreasing the possibility of genetic recombination between vector and packaging constructs and the generation of replication-competent viruses. Using described LVs based on human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus MAC251 (SIVMAC251), we asked whether heterologous virion particles in which trans-acting factors belonged to HIV-1 and cis elements belonged to SIVMAC251 (HIV-siv) would behave as parental homologous vectors in all cell types. To our surprise, we found that although the heterologous HIV-siv vector was as infectious as its homologous counterpart in most human cells, it was defective in the transduction of dendritic cells (DCs) and, to a lesser extent, macrophages. In DCs, the main postentry defect was observed in the formation of two-long-terminal-repeat circles, despite the fact that full-length proviral DNA was being synthesized and was associated with the nucleus. Taken together, our data suggest that heterologous HIV-siv vectors display a cell-dependent infectivity defect, most probably at a post-nuclear entry migration step. As homologous HIV and SIV vectors do transduce DCs, we believe that these results underscore the importance of a conserved interaction between cis elements and trans-acting viral factors that is lost or suboptimal in heterologous vectors and essential only in the transduction of certain cell types. For gene therapy purposes, these findings indicate that the cellular tropism of LVs can be modulated not only through the use of distinct envelope proteins or tissue-specific promoters but also through the specific combinatorial use of packaging and transfer vector constructs.

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Restoration of Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Responses in CD8+ T Cells from Late-Stage Patients on Prolonged Antiretroviral Therapy by Stimulation In Vitro with HIV-1 Protein-Loaded Dendritic Cells

Journal of Virology ◽

10.1128/jvi.75.9.4413-4419.2001 ◽

2001 ◽

Vol 75 (9) ◽

pp. 4413-4419 ◽

Cited By ~ 26

Author(s):

Zheng Fan ◽

Xiao-Li Huang ◽

Luann Borowski ◽

John W. Mellors ◽

Charles R. Rinaldo

Keyword(s):

Human Immunodeficiency Virus ◽

T Cells ◽

Dendritic Cells ◽

Antiretroviral Therapy ◽

Immunodeficiency Virus ◽

Ifn Γ ◽

Anti Hiv ◽

Hiv 1

ABSTRACT We demonstrate that dendritic cells loaded in vitro with human immunodeficiency virus type 1 (HIV-1) protein-liposome complexes activate HLA class I-restricted anti-HIV-1 cytotoxic T-lymphocyte and gamma interferon (IFN-γ) responses in autologous CD8+ T cells from late-stage HIV-1-infected patients on prolonged combination drug therapy. Interleukin-12 enhanced this effect through an interleukin-2- and IFN-γ-mediated pathway. This suggests that dendritic cells from HIV-1-infected persons can be engineered to evoke stronger anti-HIV-1 CD8+ T-cell reactivity as a strategy to augment antiretroviral therapy.

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HIV-1 Tat Promotes Integrin-Mediated HIV Transmission to Dendritic Cells by Binding Env Spikes and Competes Neutralization by Anti-HIV Antibodies

PLoS ONE ◽

10.1371/journal.pone.0048781 ◽

2012 ◽

Vol 7 (11) ◽

pp. e48781 ◽

Cited By ~ 34

Author(s):

Paolo Monini ◽

Aurelio Cafaro ◽

Indresh K. Srivastava ◽

Sonia Moretti ◽

Victoria A. Sharma ◽

...

Keyword(s):

Dendritic Cells ◽

Hiv Transmission ◽

Hiv Antibodies ◽

Anti Hiv ◽

Hiv 1

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TSAO-T Analogues Bearing Amino Acids at Position N-3 of Thymine: Synthesis and Anti-Human Immunodeficiency Virus Activity

Antiviral Chemistry and Chemotherapy ◽

10.1177/095632020001100106 ◽

2000 ◽

Vol 11 (1) ◽

pp. 61-69 ◽

Cited By ~ 3

Author(s):

C Chamorro ◽

E De Clercq ◽

J Balzarini ◽

M-J Camarasa ◽

A San-Félix

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Inhibitory Effect ◽

Acid Moiety ◽

Hiv Replication ◽

Virus Activity ◽

Immunodeficiency Virus ◽

Amino Acid Moiety ◽

Anti Hiv ◽

Hiv 1

Novel analogues of the anti-HIV-1 lead compound [1-[2‘,5’-bis- O-( tert-butyldimethylsilyl)-β-D-ribofuranosyl]thymine]-3‘-spiro-5’-(4“-amino-1”,2“-oxathiole-2‘,2’-dioxide) (TSAO-T) bearing different amino acids at position N-3 of thymine were prepared and evaluated as inhibitors of HIV replication. The synthesis of the target compounds was accomplished by coupling of the appropriate TSAO intermediate with a conveniently protected (L) amino acid in the presence of BOP and triethylamine, followed by depro-tection of the amino acid moiety. Several TSAO derivatives, bearing at N-3 position of the thymine base an L-amino acid retaining the free carboxylic acid, acquired activity against HIV-2, in addition to their inhibitory effect on HIV-1.

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