MOLECULAR STUDY OF HUMAN HEAD LICE, PEDICULUS HUMANUS CAPITIS COMPARISON WITH GOAT SUCKING LICE, LINOGNATHUS STENOPSIS (BURMEISTER) STRAINS

2020 ◽  
pp. 132-139
Keyword(s):  
Genetic Difference ◽  
Human Head ◽  
Molecular Study ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
School Students ◽  
Pediculus Humanus ◽  
Sucking Lice ◽  
Pcr Products ◽  
Amplicon Size

In this study, only (122) out of (915) primary school students were shown to be infected with head lice Pediculus. humanus capitis. The number and percentage of infected males were 46 (11.3%), while the number and percentage of infected females were 76 (14.9%). The results in our study also showed that the number and percentage of goats infected with goat sucking lice, Linognathus stenopsis was 70 (21.7%) of the total 322 animals, with the highest number and percentage among female goats 44 (62.9%) compared to the male goats 26 (37.1%). The study demonstrated that the rate of genetic difference between the studied samples was 89% and the similarity rate was 11%. Detection of OP-K01 gene pieces by PCR products showed that the amplicon size was 520 bp for P. humanus capitis isolated from humans, while the detection of OP-E20 and OP-M05 gene pieces with PCR product showed the lowest amplicon size 230 bp for Linognathus stenosis isolated from goats.

Pyrethroid Pediculicide Resistance of Head Lice in Canada Evaluated by Serial Invasive Signal Amplification Reaction

2010 ◽  
Vol 14 (3) ◽  
pp. 115-118 ◽  
Author(s):  
Danielle Marcoux ◽  
Kathleen G. Palma ◽  
Nalini Kaul ◽  
Hilliary Hodgdon ◽  
Andrea Van Geest ◽  
...  
Keyword(s):  
Allele Frequency ◽  
Signal Amplification ◽  
Head Louse ◽  
Human Head ◽  
The United States ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
Pediculus Humanus ◽  
S Allele ◽  
Amplification Reaction

Background: Most people in the United States and Canada with pediculosis will be treated with neurotoxic pediculicides containing pyrethrins or pyrethroids. Their widespread use led to significant resistance reported from various countries. Although treatment failures are frequently observed in Canada, the resistance frequency to pyrethroid pediculicide of human head lice (Pediculus humanus capitis) has not been determined. Objective: To determine the knockdown resistance ( kdr) allele frequency in human head louse populations in Canada. Methods: Patients infested with Pediculus humanus capitis, aged 4 to 65 years, residents of Ontario, Quebec, and British Columbia, were participants. Head lice were collected by combing and picking the enrolled subjects' hair. Lice were analyzed by serial invasive signal amplification reaction (SISAR) for genotyping the T917I mutation of lice indicating permethrin resistance. The permethrin-resistant kdr allele (R allele) frequency could then be evaluated in the head lice collected in Canada. Results: Of the head louse populations analyzed, 133 of 137 (97.1%) had a resistant (R) allele frequency, whereas only 4 of 137 (2.9%) had a susceptible (S) allele frequency. Conclusions: The 97.1% resistant (R) allele frequency in head lice from Canada could explain the treatment failures encountered with pyrethrin and pyrethroid pediculicide treatments in Canadian populations infested with Pediculus humanus capitis as the latter will not be eliminated by those pediculicides.

scholarly journals Uji Efektivitas Formula Pedikulosida Berbahan Aktif Minyak Atsiri Terhadap Mortalitas Kutu Kepala (Pediculus humanus capitis De Geer) Secara In Vitro

2018 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Tanendri Arrizqiyani
Keyword(s):  
Essential Oils ◽  
Petri Dish ◽  
Human Head ◽  
Test Method ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
Pediculus Humanus ◽  
Randomized Design ◽  
Negative Side Effects

Pediculosis is a disease caused by Pediculus humanus capitis de Geer or often called head lice which is an obligate ectoparasite in the human head. The prevalence of pediculosis is quite high and there are reports of resistance and negative side effects regarding the use of synthetic pediculosides trigger research and development of vegetable pediculosides. This study aims to examine the effectiveness of the formula of pediculoside with active ingredients of essential oils on head lice mortality in vitro. The study was conducted by testing 3 formulas with variations in the composition of essential oils made from cassava oil, fennel oil, lemon oil, water and vegetable glycerine. Comparison of the ingredients in formula A is 10%, 10%, 5%, 50% and 20%; formula B is 5%, 15%, 5%, 50% and 20%; while formula C is 15%, 5%, 5%, 50% and 20%. The test method is an experiment with a completely randomized design. The test is done by placing 10 adult head lice on filter paper which has been saturated with a formula made in a petri dish. Mortality of head lice is observed based on time. Test results data will be compared with negative controls and positive controls in the form of testing using permethrin-active pediculoside drugs. The results showed that the pediculoside formula A, B and C had a high mortality rate which caused 100% of the deaths of head lice tested in less than 5 minutes. Statistically there is a significance to the results of testing formulas A, B, and C when compared to controls. Based on the results of multiple statistical statistics, it was found that formula C was the most effective as a pediculoside formula

First Determination of Pyrethroid Knockdown Resistance Alleles in Human Head Lice (Phthiraptera: Pediculidae) From Chile

2019 ◽  
Vol 56 (6) ◽  
pp. 1698-1703 ◽  
Author(s):  
Gonzalo Roca-Acevedo ◽  
Carmen Paz del Solar Kupfer ◽  
Paulina Dressel Roa ◽  
Ariel Ceferino Toloza
Keyword(s):  
Binding Sites ◽  
Selective Pressure ◽  
Pyrethroid Resistance ◽  
Public Health Problem ◽  
Human Head ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
Over The Counter ◽  
Pediculus Humanus

AbstractThe infestation with the human ectoparasite, Pediculus humanus capitis (De Geer), is a common public health problem affecting schoolchildren worldwide. In Chile, the main active ingredients present in the over-the-counter pediculicides contain pyrethroids. Despite the extended use of these products, there is no evidence of the insecticide resistance status of the head lice geographically located in Chile. The most extended resistant mechanism of pyrethroids consists of the target site insensitivity (Kdr) determined by the presence of mutations linked to insecticide-binding sites in the voltage-sensitive sodium channel. T917I is recognized as the main mutation in head lice, and detection is considered to be a biomarker of resistance. The goal of the present study was to detect the presence and distribution of T917I mutation in five geographic locations of Chile. All five geographically selected louse populations had a frequency of pyrethroid resistance genes that ranged from 36 to 77%, and 94.9% of the collected head lice had one or two T917I mutant alleles. Moreover, the frequency of the aggregate resistant alleles was 50.5%. This is the first evidence that head lice in Chile had the mutations commonly associated with the resistance to pyrethroids. Moreover, the overrepresentation of heterozygotes in the studied populations suggests that head lice in Chile are currently under active selective pressure.

Geographic Distributions and Origins of Human Head Lice (Pediculus humanus capitis) Based on Mitochondrial Data

2008 ◽  
Vol 94 (6) ◽  
pp. 1275-1281 ◽  
Author(s):  
Jessica E. Light ◽  
Julie M. Allen ◽  
Lauren M. Long ◽  
Tamar E. Carter ◽  
Lisa Barrow ◽  
...  
Keyword(s):  
Human Head ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
Pediculus Humanus ◽  
Mitochondrial Data ◽  
Geographic Distributions

scholarly journals Molecular analysis of mitochrondrial cytb of Pediculus humanus capitis in Thailand revealed potential historical connection with South Asia

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257024
Author(s):  
Kobpat Phadungsaksawasdi ◽  
Sakone Sunantaraporn ◽  
Nirin Seatamanoch ◽  
Switt Kongdachalert ◽  
Atchara Phumee ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Phylogenetic Tree ◽  
South Asia ◽  
Molecular Analysis ◽  
Human Head ◽  
Population Migration ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
Pediculus Humanus ◽  
Clade C

Background Pediculus humanus capitis or head louse is an obligate ectoparasite and its infestation remains a major public health issue worldwide. Molecular analysis divides head lice into six clades and intra-clade genetic differences have been identified. Several hypotheses have been formulated to elucidate the discrepancies of the variety of head lice among different regions of the world. It is currently concluded that head lice distribution might be associated with human migration history. This study aims to investigate genetic data of human head lice in Thailand. We believe that the analysis could help establish the correlation between local and global head lice populations. Method We investigated mitochondrial cytochrome b (cytb) gene of the collected 214 head lice to evaluate genetic diversity from 15 provinces among 6 regions of Thailand. The head lice genes were added to the global pool for the phylogenetic tree, Bayesian tree, Skyline plot, and median joining network construction. The biodiversity, neutrality tests, and population genetic differentiation among the 6 Thailand geographic regions were analyzed by DNAsp version 6. Results The phylogenetic tree analysis of 214 collected head lice are of clade A and clade C accounting for roughly 65% and 35% respectively. The Bayesian tree revealed a correlation of clade diversification and ancient human dispersal timeline. In Thailand, clade A is widespread in the country. Clade C is confined to only the Central, Southern, and Northeastern regions. We identified 50 novel haplotypes. Statistical analysis showed congruent results between genetic differentiation and population migration especially with South Asia. Conclusions Pediculosis remains problematic among children in the rural areas in Thailand. Cytb gene analysis of human head lice illustrated clade distribution and intra-clade diversity of different areas. Our study reported novel haplotypes of head lice in Thailand. Moreover, the statistic calculation provided a better understanding of their relationship with human, as an obligate human parasite and might help provide a better insight into the history of human population migration. Determination of the correlation between phylogenetic data and pediculicide resistance gene as well as residing bacteria are of interest for future studies.

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