scholarly journals The chemistry, pharmacology, and toxicology of the biologically active constituents of the herb Hypericum perforatum L.

2003 ◽  
Vol 1 (2) ◽  
pp. 61-70 ◽  
Author(s):  
Jiří Patočka
Keyword(s):  
Hypericum Perforatum ◽  
Biologically Active ◽  
Active Constituents ◽  
Hypericum Perforatum L ◽  
Pharmacology And Toxicology

Antilipoxygenase activity of compounds from Hypericum perforatum

Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Marek Obložinský ◽  
Lýdia Bezáková ◽  
Ivana Holková ◽  
Marián Vanko ◽  
Thomas Kartnig ◽  
...  
Keyword(s):  
Hypericum Perforatum ◽  
Allergic Diseases ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
Active Constituents ◽  
Ethanolic Extracts ◽  
Key Enzyme ◽  
Hypericum Perforatum L ◽  
Hypericum Species

AbstractThe main biologically active constituents of Hypericum species are flavonoids (quercetin, isoquercitrin, hyperoside, rutin), biflavonoids and naphthodianthrones (hypericin, pseudohypericin). Lipoxygenase is the key enzyme in the biosynthesis of leukotriens, which have been postulated to play an important role in the pathophysiology of several inflammatory and allergic diseases. This work deals with the investigation of potential antilipoxygenase activity of different compounds and extracts isolated from Hypericum perforatum L. The highest inhibitory effect was exhibited by flavonoid derivative hyperoside (IC50 5.768 × 10−6 M). Acetone and ethanolic extracts caused also an inhibition of lipoxygenase. On the basis of inhibitory effect of compounds tested we assume that the most of them may be involved in the antiinflammatory principles of Hypericum perforatum L.

scholarly journals VITAMINS AND ALKALOIDS IN SOME MEDICINAL PLANTS (ACHILLEA MILLEFOLIUM L., CAPPARIS SPINOSA L., HYPERICUM PERFORATUM L.) AND THEIR EFFECTS ON HUMAN HEALTH

2022 ◽  
Author(s):  
EditorJournals and Conferences
Keyword(s):  
Chemical Composition ◽  
Medicinal Plants ◽  
Human Health ◽  
Hypericum Perforatum ◽  
The Body ◽  
Biologically Active ◽  
Physiological Characteristics ◽  
Achillea Millefolium ◽  
Capparis Spinosa ◽  
Hypericum Perforatum L

This article provides information on the morpho-physiological characteristics, chemical composition, especially the amount of alkaloids and vitamins, and their function in the body, as well as thorn bushes, hole field, yarrow plants, which are considered medicinal plants.The article deals with the botanical and morpho-physiological characteristics of the plant Achillea Millefolium L., one of the medicinal plants used in scientific medicine, systematics, some biologically active substances in the chemical composition, especially vitamins and alkaloids, their function in the body and their role in human health. details are given.The following article describes the role of Capparis spinosa L. in modern taxonomy, its distribution, the structure and properties of the most common alkaloids and vitamins in the plant, and its biochemical functions in the human body.The article concludes with a detailed description of the morpho-physiological characteristics of the plant Hypericum Perforatum L., its chemical composition, especially the amount of alkaloids and vitamins and their function in the body and their role in human health.

scholarly journals Decoupling of Plant Growth and Accumulation of Biologically Active Compounds in Leaves, Roots, and Root Exudates of Hypericum perforatum L. by the Combination of Jasmonate and Far-Red Lighting

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1283 ◽  
Author(s):  
Martina Paponov ◽  
Manya Antonyan ◽  
Rune Slimestad ◽  
Ivan A. Paponov
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Root Exudates ◽  
Hypericum Perforatum ◽  
Secondary Compounds ◽  
Biologically Active ◽  
Starch Accumulation ◽  
Combined Application ◽  
Hypericum Perforatum L ◽  
Spraying Plants

The plant hormone jasmonic acid (JA) fine tunes the growth–defense dilemma by inhibiting plant growth and stimulating the accumulation of secondary compounds. We investigated the interactions between JA and phytochrome B signaling on growth and the accumulation of selected secondary metabolites in Hypericum perforatum L., a medically important plant, by spraying plants with methyl jasmonate (MeJA) and by adding far-red (FR) lighting. MeJA inhibited plant growth, decreased fructose concentration, and enhanced the accumulation of most secondary metabolites. FR enhanced plant growth and starch accumulation and did not decrease the accumulation of most secondary metabolites. MeJA and FR acted mostly independently with no observable interactions on plant growth or secondary metabolite levels. The accumulation of different compounds (e.g., hypericin, flavonols, flavan-3-ols, and phenolic acid) in shoots, roots, and root exudates showed different responses to the two treatments. These findings indicate that the relationship between growth and secondary compound accumulation is specific and depends on the classes of compounds and/or their organ location. The combined application of MeJA and FR enhanced the accumulation of most secondary compounds without compromising plant growth. Thus, the negative correlations between biomass and the content of secondary compounds predicted by the growth-defense dilemma were overcome.

Prenylated β-diketones, two new additions to the family of biologically active Hypericum perforatum L. (Hypericaceae) secondary metabolites

2018 ◽  
Vol 118 ◽  
pp. 505-513 ◽  
Author(s):  
Niko S. Radulović ◽  
Marija S. Genčić ◽  
Nikola M. Stojanović ◽  
Pavle J. Randjelović ◽  
Nicolas Baldovini ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Hypericum Perforatum ◽  
Biologically Active ◽  
The Family ◽  
Hypericum Perforatum L

TLC-Direct Bioautography as a Bioassay Guided Method for Investigation of Antibacterial Compounds in Hypericum perforatum L.

2015 ◽  
Vol 98 (4) ◽  
pp. 1013-1020 ◽  
Author(s):  
Wioleta Jesionek ◽  
Ágnes M Móricz ◽  
Ágnes Alberti ◽  
Péter G Ott ◽  
Béla Kocsis ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Hypericum Perforatum ◽  
Micrococcus Luteus ◽  
Biological Properties ◽  
Biologically Active ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Antimicrobial Substances ◽  
Hypericum Perforatum L ◽  
Direct Bioautography

Abstract Fast high-throughput TLC-direct bioautography (DB) is an effect-directed analysis method that enables searching for biologically active (e.g., antimicrobial) substances in complex mixtures like plant extracts. The principle of the method is that separation and detection of biological properties of given mixture components is performed directly on a TLC plate. In searching for antibacterial activity, the developed plate is immersed in a bacterial broth, and bacteria grow directly on its layer during a proper incubation time. Inhibition zones are formed in places where antimicrobial components are located. The active compounds can be further identified using spectroscopic techniques. The aim of our study was investigation of plant components of Hypericum perforatum L. tincture by TLC-DB using nine bacterial strains: Micrococcus luteus, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant S. aureus, S. epidermidis, Pseudomonas syringae pv. maculicola, Xanthomonascampestris pv. vesicatoria, and Aliivibrio fischeri. Compounds showing the widest range of antimicrobial activity were isolated using semipreparative TLC and identified as apigenin, 3,8'-biapigenin, quercetin, kaempferol, and linolenic acid by TLC, HPLC-diode array detection, and HPLC/MS/MS techniques.

scholarly journals Simultaneous Determination of the Predominant Hyperforins and Hypericins in St. John's Wort (Hypericum perforatum L.) by Liquid Chromatography

2000 ◽  
Vol 83 (4) ◽  
pp. 944-949 ◽  
Author(s):  
Dean E Gray ◽  
George E Rottinghaus ◽  
H E Gene Garrett ◽  
Stephen G Pallardy
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Hypericum Perforatum ◽  
Dry Weight ◽  
Active Constituents ◽  
St John’S Wort ◽  
St John's Wort ◽  
Relative Standard ◽  
Hypericum Perforatum L

Abstract Hypericin and hyperforin are believed to be among the active constituents in common St. John's wort (Hypericum perforatum L.). Presently, dietary supplements are generally standardized to contain specified levels of hypericin and hyperforin, and the related compounds, pseudohypericin and adhyperforin. A rapid method was developed for simultaneous determination of these 4 active constituents by liquid chromatography (LC). A 1 g portion of dried, finely ground leaf/flower sample is extracted with 20 mL methanol for 2 h. A 0.6 mL aliquot of the crude extract is combined with 5.4 mL acetonitrile–methanol (9 + 1) and passed through a mixed solid-phase cleanup column. The eluate is examined by LC for hyperforin, adhyperforin, hypericin, and pseudohypericin on a Hypersil reversed-phase column by using simultaneous ultraviolet (284 nm) and fluorescence detection (excitation, 470 nm; emission, 590 nm). The compounds are easily separated isocratically within 8 min with a mobile phase of acetonitrile–aqueous 0.1M triethylammonium acetate (8 + 2). Average recoveries of hyperforin and adhyperforin were 101.9 and 98.4%, respectively, for 3 sample mixtures containing concentrations ranging from approximately 0.2 to 1.5% combined hyperforins per gram dry weight. Average relative standard deviation (RSD) values for hyperforin and adhyperforin for all 3 mixtures were 18.9 and 18.0%, respectively. Average recoveries of hypericin and pseudohypericin were 88.6 and 93.3% respectively, from 3 sample mixtures containing concentrations ranging from approximately 0.2 to 0.4% combined hypericins per gram dry weight. Average RSD values for hypericin and pseudohypericin for all 3 mixtures were 3.8 and 4.2%, respectively.

Determination of Hypericin in St. John's Wort (Hypericum perforatum L.) extracts using HPLC-ED

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
E Sofic ◽  
A Copra Janicijevic ◽  
M Maksimovic ◽  
I Tahirovic ◽  
L Klepo ◽  
...  
Keyword(s):  
Hypericum Perforatum ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L
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