scholarly journals Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.)

2015 ◽  
Vol 60 (3) ◽  
pp. 263-275 ◽  
Author(s):  
Fardin Nasri ◽  
Arsalan Fadakar ◽  
Mahmood Saba ◽  
Bayzid Yousefi

Rosa damascena is very important for essential oil production, medicinal properties and it is also widely cultivated as a garden rose. The Rose species is mainly propagated by stem cutting. In the present study, the effect of different levels of 0, 500 and 1,000 mg l-1 (quick dip method for 20 s) of indole butyric acid (IBA) on the rooting of 12 wild genotypes (including: Kurdistan 1 to Kurdistan 12) of R. damascena was investigated. The results show that the rooting ability of R. damascena differs significantly between the twelve genotypes. The highest rooting (79.56%) and callus production (69.08%), number of roots (8.33), root fresh and dry weights (361.80 and 244.74 mg, respectively) were recorded in Kurdistan 5 genotype with 1,000 mg l-1 IBA. The maximum root length (5.84 cm) was observed in Kurdistan 5 genotype with 500 mg l-1 IBA that showed a significant difference compared to the control treatment (0.96 cm). The highest number of leaves per bud (7.33 at 500 mg l-1 IBA) and number of buds (5.00 at 1,000 mg l-1 IBA) were recorded in Kurdistan 1 genotype. The current study demonstrated that the different genotypes of R. damascena were in a difficult-toroot state, which suggests that cutting treatment with 1,000 mg l-1 IBA overcame the problem of the difficult-to-root state, and it can also enhance the rooting percentage in the studied genotypes.


1995 ◽  
Vol 9 (4) ◽  
pp. 736-740
Author(s):  
Sangwook Han ◽  
Thomas W. Fermanian ◽  
Thomas B. Voigt

With the increased development of new tall fescue cultivars used in turf, it is important to understand their individual response to herbicide treatment. The effect of prodiamine on tall fescue root dry weight and root length of selected tall fescue cultivars was studied in the greenhouse in 1.3-m-deep pots of calcined clay. Prodiamine at 0.8 kg ai/ha did not significantly affect either root dry weight or root length. However, the mean root dry weight and maximum root length averaged over all cultivars were significantly reduced at 4 wk after treatment with 1.7 kg/ha. When the prodiamine treatments were repeated in a second experiment, both rates caused a significant reduction in the mean root dry weight and maximum root length but there was no significant difference between prodiamine rates. Single degree of freedom contrasts between the untreated and treated turfs for each cultivar had some differential response in root dry weight. The reduction in root dry weight in the prodiamine treatments was more pronounced in the second study because the turf was less mature. ‘Olympic’ and ‘Rebel’ tall fescue had significantly reduced root dry weight at the 1.7 kg/ha rate in first study; whereas, ‘Amigo,’ Olympic, ‘Sundance,’ and ‘Taurus' tall fescue had significant reduction in root dry weight at both 0.8 and 1.7 kg/ha prodiamine rates. ‘Midnight’ Kentucky bluegrass had significantly reduced root length at both prodiamine rates in the second experiment but in general there was little difference among tall fescue cultivars treated with prodiamine.



2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Rizky Wulandari ◽  
Yaya Hasanah ◽  
Meiriani Meiriani

Using fruit branch for pepper shrub propagation is one of alternatives for an efficient pepper multiplication which usually uses underlayer cuttings. This research is aimed at finding the growth response of two pepper (Piper nigrum L.) cuttings to the administration of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid). This research was conducted at the greenhouse of the Faculty of Agriculture, University of Sumatera Utara, Medan (± 32 m above sea level), from April to August 2017 using a factorial randomized block design with 2 factors, pepper branch cuttings (primary branch cuttings and secondary branch cuttings) and the administration of IBA and NAA (0+0 ppm, 2500+0 ppm, 0+2500 ppm, 1500+1000 ppm, and 1000+1500 ppm). The results show that the emerging shoot rate in the secondary branch cuttings was significantly faster than in the primary branch cuttings, but the volume of root in the primary branch cuttings is significantly larger than in the secondary branch cuttings. There was no significant difference in the administration of IBA and NAA on all observed variables. The highest interaction of shoot length was found in the  primary branch cuttings with the administration of  IBA 1500 ppm + NAA 1000 ppm and the highest percentage of root and root volume was found in the primary branch cuttings with the administration of IBA 2500 ppm + NAA 0 ppm.



2020 ◽  
Vol 37 (1) ◽  
pp. 109-121
Author(s):  
C.U. Okafor ◽  
E.U. Njoku ◽  
F.C. Ike ◽  
C.C. Onyekwuluje

Field regeneration of three (3) medicinal plants - Securidaca longepedunculata (violet tree), Ocimum gratissimum (scent leaf) and Pterocarpus mildebraedii by means of rooting of stem cuttings at different lengths of 10 -15 cm and 15 -20 cm were studied. The effect of the plant hormone Indole-3-Butyric acid (IBA) at 5g/l was tested on rooting, bud sprout and leaf formation of the rooted stem cuttings of the plant species as well as the effect of the length of the stem cuttings on their growth and survival rates. The results of the study revealed that stem cuttings of O. gratissimum treated with IBA and the control treatment sprouted within 5 - 8 days. The treated O. gratissimum stem cuttings produced a slightly higher number of buds (2.58 ± 0.86) when compared to the control (2.00 ± 0.89) with no significant difference at P ≤ 0.05. Stem cuttings of the treated P. mildbraedii cuttings sprouted within 8 -10 days with 1.40 ± 0.37 number of buds, with the control showing no sprouts. Consequently, IBA application produced more leaves (13.00 ± 5.58) and roots (1.33 ± 0.01) than the control. However, O. gratissimum stem cuttings had the highest leaf (13.08 ± 4.47) and root numbers (135.00 ± 13.45) followed by the control with values- 13.00 ± 5.58 for leaf development and 61.66 ± 2.34 for root number respectively. Irrespective of the treatments, none of the stem cuttings of S. longepedunculata sprouted. This study showed that exogenous application of IBA to stem cuttings of the tested plants, except S. Longepedunculata, improved the root number, number of leaves and number of buds. Keywords: Stem cuttings, Indole-3-Butyric acid (IBA), Rooting



Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 197
Author(s):  
Pinki Devi ◽  
Lisa Wasko DeVetter ◽  
Scott Lukas ◽  
Carol Miles

This study evaluated the use of splice grafting as a propagation strategy for watermelon. In experiment 1, the treatments consisted of sucrose, antitranspirant A, antitranspirant B, auxin (indole-3-butyric acid (IBA)) at two concentrations (10 and 20 mgL−1), plus a water control. The survival (%) of splice-grafted watermelon plants differed due to the number of days after grafting and treatment (p < 0.0001, for both). At 21 days after grafting, plants treated with sucrose and antitranspirant A, and sucrose and antitranspirant A with 10 mgL−1 auxin had 90% and 88% survival, respectively, whereas the graft survival was 18% for plants treated with water. Experiment 2 included the three top performing treatments from experiment 1 and a water control treatment, applied to both root-intact and root-excised rootstocks. There was a significant difference in survival (%) of splice-grafted watermelon due to root treatments, exogenous treatments, and the number of days after grafting (p < 0.0001, for all). At 21 days after grafting, survival for root-excised grafted plants was 11% lower compared to root-intact plants. Plants treated with sucrose and antitranspirant A, and sucrose and antitranspirant A with 10 mgL−1 auxin had 87% and 86% survival, respectively, whereas plants treated with water had 14% survival. The external application of auxin applied to rootstock seedlings does not appear to be cost-effective; however, other products should be evaluated.



2020 ◽  
Author(s):  
Javaid Iqbal ◽  
Bushra Khan ◽  
Sardar Khan ◽  
Nasreen Ghaffar ◽  
Ishaq Ahmad Mian ◽  
...  

Abstract Background Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan. Results The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings. Conclusion The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.



HortScience ◽  
2018 ◽  
Vol 54 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Haishan An ◽  
Jiajia Meng ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xiaoqing Wang ◽  
...  

Vegetative propagation by cuttings is a very popular method. However, blueberry propagation using cuttings is still a main factor limiting its expansion because its results can vary according to the blueberry cultivar and environmental factors. This study aimed to evaluate the rooting abilities of hardwood cuttings for six blueberry cultivars (O’Neal, Misty, Diana, Biloxi, Bluebeauty, and Coville) using three different exogenous indole-butyric acid (IBA) concentrations (1000, 2000, and 3000 ppm), and to determine if the cutting position (basal, central, apical) affects rooting performance. A control treatment (0 ppm IBA) was also performed. After 90 days of each treatment, rooting percentage, average root length, and average root number per cutting were assessed and used to calculate rooting index, which is a measure of rooting ability. The rooting percentages of hardwood cuttings differed largely among cultivars and were highest for ‘Bluebeauty’ (68.55%), followed by ‘Biloxi’ (68.01%). The rooting index values of these two cultivars (33.59 and 35.18, respectively) were significantly higher than those of the other four cultivars. The rooting response of blueberry hardwood cuttings to IBA concentrations was quadratic, and 1000 and 2000 ppm IBA were sufficient to express the maximum rooting percentage in most cultivars. The rooting abilities of basal, central, and apical cuttings were similar with treatments with high IBA concentrations. The effects of the cultivar, IBA concentration, and interaction between them on rooting percentage, average root length, and average root number were significant; however, the effects of the cutting position on the rooting percentage and average root length were not. This suggested that the rooting abilities of blueberry hardwood cuttings were significantly influenced by the cultivar and IBA concentration rather than by the cutting position.



2020 ◽  
Vol 26 (2) ◽  
Author(s):  
Nandini Kumari ◽  
Pushpanjali Khare

A pot experiment was conducted to observe the effect of indigenous strains of rhizobacteria isolated from litchi orchards of East Champaran, Bihar (India) on the growth and survivability of litchi seedlings. Three efficient native rhizobacterial isolates belonging to genera Pseudomonas, Azotobacter and Ochrobactrum were used in seven different combinations and their effect on germination rate, shoot height, root length, number of leaves were observed and recorded in litchi seedlings. Pot 7 containing mixed combination of all the three isolates in equal proportion showed maximum root length (11.7 cm) and shoot length (12.5 cm), early germination as compared to control Pot P8 devoid of any isolates. Pot 4 (Pseudomonas + Azotobacter) and pot 5 (Pseudomonas + Ochrobactrum) also gave satisfactory result in all the parameters recorded. The results clearly state that, mixture of all the three rhizobacterial isolates as “Biomix” produced stimulatory growth results on litchi seedlings as compared to sole application and control. This “Biomix” could be used for formulating effective biofertilizers for litchi orchards in future.



2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Annamalai Anandan ◽  
Chidambaranathan Parameswaran ◽  
Anumalla Mahender ◽  
Amaresh Kumar Nayak ◽  
Sampthamprajan Vellaikumar ◽  
...  

AbstractTo better understand the early response of genotypes to limited-phosphorus (P) conditions and the role of the phosphate transporter OsPHT1 gene family in the presence of PSTOL1, it is essential to characterize the level of tolerance in rice under limited-P conditions. In the present experiment, six rice genotypes were studied in three-way interactions [genotype (G) × phosphorus (P) × duration (D)] by comparing them at two instances (14 d and 28 d) under seven different concentrations of P (0.5‒10.0 ppm) in a hydroponic system. Trait differences and interactions of these traits were clearly distinguished among the various P rates. However, aboveground trait expression registered increased growth from 6.0 to 10.0 ppm of P. The major root-attributed traits in 0.5 ppm of P are significantly increased vis-à-vis 10 ppm of P. Analysis of variance displayed a significant difference between the genotypes for PSTOL1 and PHT1 expression. In low P, maximum root length with a shoot and root dry weight was observed in a new indigenous accession, IC459373, with higher expression of PSTOL1 than in Dular and IR64-Pup1 in 0.5 ppm of P at 14 d. Among the 13 PHT1 genes, OsPT1, OsPT2, OsPT6, and OsPT13 showed significant upregulation in IC459373, Dular, and IR64-Pup1. These results indicated that studying the expression levels of the PSTOL1 and PHT1 gene family at the early growth stages would be helpful in identifying superior donors to improve low-P tolerance and P-use efficiency in rice breeding programs.



2007 ◽  
Vol 4 (2) ◽  
pp. 233-238
Author(s):  
Baghdad Science Journal

Shoot tip of Actinidia chinensis plant were excised and sterilized with mercuric chloride (Hgcl2) at concentration 0.1% for 5 min, then cultured on MS medium supplemented with benzeladinine BA at concentration (0.0,0.5,1.0,2.0,2.5) mg/l in multiplication stage Moreover, indole butyric acid IBA at concentration (0.0, 0.5,1.0,2.0,4.0) mg/l and (0.0,0.5,1.0,2.0) mg/l on solid and liquid solution media respectively in rooting stage were studied. All experiments have done under controlled conditions (25 ±2) Cº and 1000Lux light intensity for 16 hrs / day. Results revealed that supplementing the media with BA at concentrations 2.0 and2.5 mg/l was superior to give highest rate of shoot and number of leaves reached 5.8 /explant and 6.0 leaves respectively. While control treatment gave highest rate of shoot lengths reached 1.99cm Moreover, including liquid media with 2.0 mg/l IBA gave highest rooting percentage(100% )as compared with the same concentration of IBA which gave rooting percentage reached 90% . Results of Acclimatization stage showed that addition of 1:1 peatmos and loamy soil was superior to give highest rate of success( 100%) after 4 weeks of acclimatization .



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