scholarly journals The Isolation and Characterization of Phenolic Compound of Euphorbia Plant/Patikan Cina (Euphorbia thymifolia Linn)

2019 ◽  
Vol 1 (1) ◽  
pp. 40-44
Author(s):  
Hilda Amanda
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Compound ◽  
Silica Gel ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Wave Number ◽  
Uv Spectrum ◽  
Isolation And Characterization ◽  
Vacuum Liquid Chromatography ◽  
Macerated Extract

Euphorbia plant, Euphorbia thymifolia Linn, is one the Euphorbiaceae families that still need to be developed due to its benefits. An effort to be able to use this plant maximumly is by studying the active component in the plant. This study aims to isolate and characterize phenolic compound in euphorbia plant extract using ethyl acetate. 500 gram of euphorbia plant (Patikan Cina) powder was macerated using methanol. Then, the macerated extract was evaporated to eliminate excess solvent and as a result, a solvent-free extract was obtained. Next, the result was partitioned with a solution of methanol : ethyl acetate (1:1) in order to get methanol and ethyl acetate extracts. The ethyl acetate extract of the partition was evaporated to get concentrated ethyl acetate extract. Next, it was isolated in a vacuum liquid and gravitational column chromatography to get pure isolate. Silica gel 60 (0.040 – 0.063 mm) and n-hexane motion phase were used in the separation of concentrated ethyl acetate in vacuum liquid chromatography. There were 16 fractions produced from the yield. Then the fractions were put in TLC. With silica gel 60 (0.2 – 0.5 mm) mesh and n-hexane mobile phase, ethyl acetate gave 5 fractions in gravitational column chromatography and fraction 3 produced a single spot. UV and IR spectroscopy were used to determine the constituents in the isolate. From UV spectrum, max is 268.97 nm. While IR data shows the presence O-H group in 3521.38 cm-1, C – H (alkane) presents at wave number 2926.45 cm-1 reinforced by the appearance of 5 other alkanes absorption at 2857.00 cm-1, 1447.31 cm-1, 1370.18 cm-1, 868.774 cm-1 and 757.887 cm-1 wavelengths. There is also C = O (carbonyl) groups in wave number 1693.19 cm-1. The identification of structures based on UV and IR spectra data has shown that the isolate is a phenolic compound.

scholarly journals ISOLATION AND CHARACTERISATION OF BERGENIN FROM ETHYL ACETATE EXTRACT OF FLUEGGEA VIROSA LEAVES

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
C. T. Agber ◽  
T. A. Tor-Anyii ◽  
J. O. Igoli ◽  
J. V. Anyam
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Amorphous Solid ◽  
Isocratic Elution ◽  
Tropical Plant ◽  
Isolation And Characterization ◽  
Accessible Method ◽  
Vacuum Liquid Chromatography ◽  
C Nmr

Bergenin is an important constituent of Flueggea virosa (Euphorbiaceae), a tropical plant with several traditional uses. While there are numerous reports on the isolation and characterization of bergenin, a rapid, high-throughput, readily accessible method for the isolation and characterization of the compound locally has not been reported. Isocratic elution of ethyl acetate extract via vacuum liquid chromatography (VLC) with methanol produced a white amorphous solid (100 mg), which was successfully isolated from 250 g of the plant. On the basis of spectral data (1H, C NMR, COSY, HSQC and HMBC) and comparison with literature reports, the structure of this solid was shown to be bergenin, a dihydroisocoumarin derivative of glucopyranosyl gallic acid.

scholarly journals THE ISOLATED COMPOUNDS FROM THE TWIGS OF MORUS ALBA L. IN DONG THAP

2016 ◽  
Vol 54 (4) ◽  
pp. 509
Author(s):  
Bùi Thị Minh Nguyệt ◽  
Tran Huynh Nguyen Khanh
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Diels Alder ◽  
Morus Alba

From the ethyl acetate extract of the Morus alba L. (Moraceae) in Dong Thap, a triterpenoid, b-amyrin-b-D-glucopyranoside (1), a Diels – Alder type adducts, mulberrofuran G (2), a benzofuran, moracin M (3) and a prenylated flavonoid, kuwanon G (4) were isolated through repeated silica gel column chromatography. Their structures were identified by physicchemical and spectrometric methods.

Isolation of the antidiarrhoeal tiliroside and its derivative from Waltheria indica leaf extract

2021 ◽  
Vol 25 (1) ◽  
pp. 86-92
Author(s):  
B.A. Ayinde ◽  
J.O. Owolabi ◽  
I.S. Uti ◽  
P.C. Ogbeta ◽  
M.I. Choudhary
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Preparative Thin Layer Chromatography ◽  
Inhibitory Effects ◽  
Aqueous Fraction ◽  
Vacuum Liquid Chromatography ◽  
Ileum Contraction ◽  
Layer Chromatography ◽  
Dose Dependent

The antidiarrhoeal effect of Waltheria indica methanol extract and fractions have been reported earlier but, the present work examined the intestinal relaxant effects of two flavonoid-phenyl propanoids isolated from the methanol extract. The active aqueous fraction was subjected to vacuum liquid chromatography using dichloromethane with increasing concentration of ethyl acetate, and that of methanol and water successively. The ten (10) fractions obtained were combined to give seven (7). The fraction 2 (C, D) was subjected to preparative thin layer chromatography on silica gel GF254 (10-40μm) using CHCl3-CH3OH (8:2) to obtain compound coded F2. Fraction 4 (F) was subjected to column chromatography using silica gel (60-120μm mesh) and eluted with  dichloromethane with increasing concentrations of methanol. Fractions 9-28 were combined and subjected to column  chromatography using chloroform with increasing concentration of methanol. The fractions 1-16 of these were purified on Sephadex LH-20 to obtain compound BAA. The identities of the two compounds were established using spectroscopic methods. The  antidiarrheal effect of compound F2 was evaluated on mice using charcoal transit (100,200, 400mg/kg), castor oil (40, 60 mg/kg)  while the two compounds were examined for their inhibitory effects on Ach-induced ileum contraction. The effects of the  compounds were compared with loperamide (3mg/kg) and atropine (80μg). Compounds F2 and BAA were identified as tiliroside and 3’’’, 5’’’-dimethoxy tiliroside respectively. Tiliroside inhibited the charcoal transition in the animals in a dose dependent pattern with 400mg/ mL eliciting 63.41% inhibition compared to 59.23% produced by loperamide. The compound also elicited significantly (P<0.05) prolonged onset of stooling and reduced the number and weight of stools produced lower than the control. The two  compounds drastically inhibited the Ach-induced contractions of the ileum. The compound, tiliroside at 10mg, completely abolished  the contraction by Ach unlike 3’’’, 5’’’-dimethoxy tiliroside which reduced the contraction to 1.92% at 20mg. The identified compounds seem to be responsible for the ethnomedicinal use of the plant in treating diarrhea.

scholarly journals Isolation and Structure Elucidation of Soulatro Coumarin From Stem Bark of Calophyllum soulattri Burm F and In Vivo Antiplasmodial Activity by Using Mice Infected by Plasmodium berghei

2011 ◽  
Vol 2 (2) ◽  
pp. 242
Author(s):  
Jamilah Abbas ◽  
Achmad Darmawan ◽  
Syafruddin Syafruddin
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Plasmodium Berghei ◽  
2D Nmr ◽  
Stem Bark ◽  
Antiplasmodial Activity ◽  
Nmr Techniques ◽  
Coumarin Compound

The soulatro coumarin compound was isolated and elucidated from the stem bark of Calophyllum soulattri Burm F, the samples were collected from Jayapura Papua Irian Island in Indonesia. Isolation process was done by maceration at room temperature in methanol, than partitioned in a mixture of n hexane-water (1:1), followed by dichloromethane-water (1:1)  and ethyl acetate-water (1:1). A portion of ethyl acetate extract was subjected to column chromatography over silica gel packed and eluted with n-hexane a gradient of ethyl acetate to 100% followed by CHCl3  in MeOH (20:1, 10 :1, 5:1, 1:1). Fraction  B (CHCl3 in MeOH 20:1) was subjected to column chromatography  over silica gel 300 mesh  and eluted with EtOAc-MeOH mixtures of increasing polarity. Faction with the same Rf valeus were combined and eluted with EtOAc-MeOH  (19:1) showed one spot on TLC. They were combined and evaporated to yield a solid than was recrystallized in mixture of CH2Cl2-methanol to give soulatro coumarin compound. The structure was determinated by spectroscopic analysis, in particular by 1D and 2D NMR techniques, from these spectra data conclution that compound is soulatro coumarin. Antimalarial assay was tested against Plasmodium berghei parasite as in vivo using 18 mices rodent wich was infected by  Plasmodium berghei parasite. The soulatro coumarin  showed activity against P. berghei with dosage 0.0005867 mM/1 kg body weight ; 0.005867 mM/1 kg bw; 0.05867 mM/1 kg bw; 0.5867 mM/1 kg bw 5.867 mM/1 kg bw and 58.67 mM/1 kg bw could inhibite growth rate of parasite = 57.32%; 63.37%; 43.02%; 53.49%; 47.67% respectively.Keywords : Antiplasmodial activity, coumarin, Calophyllum soulattri Burm. F, in vivo, Chloroquine, Plasmodium berghei.

scholarly journals Isolation and Elucidation Structure of 28-Hydroxy-3-Friedelanone of Nyamplung (Callophyllum inophyllum, Linn.) Leaves

2020 ◽  
Vol 9 (2) ◽  
pp. 117-125
Author(s):  
Triana Kusumaningsih ◽  
◽  
Muhammad Widyo Wartono ◽  
Nais Puji Wijanarti ◽  
◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Nonpolar Solvent ◽  
Ether Extract ◽  
Diethyl Ether Extract ◽  
Isolated Compound

The isolation triterpenoid from Nyamplung (Callophyllum inophyllum, Linn.) leaves has been conducted. The isolation was employed by maceration using ethanol as solvent and liquids extraction using ethyl acetate. Ethyl acetate extract was partitioned successively using nonpolar solvent with hexane, dichloromethane, and diethyl ether, respectively. The diethyl ether extract was purified by column chromatography. The isolated compound of fraction D1 was obtained as white solids crystal with yield of 0.0035%. The isolated compound was determined based on the FTIR, 1HNMR, 13CNMR, HSQC, and HMBC spectra. The isolated compound was identified as 28-hydroxy-3-friedelanone.

scholarly journals ANTI DIABETIC FLAVANONE COMPOUND FROM THE LEAVES OF Artocarpus communis

2010 ◽  
Vol 9 (3) ◽  
pp. 505-508 ◽  
Author(s):  
Puspa D.N. Lotulung ◽  
Sofa Fajriah ◽  
Andini Sundowo ◽  
Euis Filaila
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Inhibition Assay ◽  
Spectroscopic Evidence

The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Artocarpus communis leaves using column chromatography techniques. The structure of the flavanone compound was elucidated on the basic of spectroscopic evidence and comparison to published values. This compound, 8-geranyl-4,5,7-trihydroxyflavone, showed strong anti diabetic activity on α-glucosidase inhibition assay with IC50 18.120 µg mL-1.   Keywords: Artocarpus communis, 8-geranyl-4,5,7-trihydroxyflavone, anti diabetic activity

scholarly journals ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 276
Author(s):  
Anisa Puspitaningrum ◽  
Berna Elya ◽  
Katrin .
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Spectrum Analysis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Uv Light ◽  
Antioxidant Compounds ◽  
Isolation And Characterization ◽  
Dpph Method

Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanolextracts of Garcinia daedalanthera Pierre leaves.Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum columnchromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined usingthe 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and itsantioxidant activity was quantitatively tested.Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 μg/mL. Identification using an AlCl3 sprayer producedyellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.Infrared spectrum analysis revealed the presence of −OH, C–H alkane, C=C aromatic, C=O, and C-O-C groups.Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibitedantioxidant activity with an IC50 of 5.82 μg/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) afterbeing sprayed with AlCl3 reagent.

scholarly journals ANTIOXIDANT ACTIVITY OF 2,6,4’-TRIHYDROXY-4-METHOXY BENZOPHENONE FROM ETHYL ACETATE EXTRACT OF LEAVES OF MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)

2011 ◽  
Vol 11 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Susilawati Susilawati ◽  
Sabirin Matsjeh ◽  
Harno Dwi Pranowo ◽  
Chairil Anwar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Chemical Constituent ◽  
Traditional Medicines ◽  
Phaleria Macrocarpa ◽  
Spherical Crystal

Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is included into family of Thymelaeaceae is one of Indonesia's traditional medicines. Chemical constituent has been isolated from ethyl acetate extract of leaves of mahkota dewa. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The ethyl acetate extract was separated and fractionated by column chromatography. The first fraction was purified by TLC preparative and recrystalization. Compound was isolated as red-brown spherical crystal in 8 mg (m.p. 129-131 °C). Its spot gave dark fluoroscence at TLC plate (UV366) with Rf of 0.3 at TLC chromatogram with eluent of n-hexane : ethyl acetate (7:3); 0.6 with n-hexane : ethyl acetate (1:1); 0.9 with -hexane : ethyl acetate (4:6). This compound was dissolved in methanol. Compound was identified by UV, IR, 1H NMR, 13C NMR and NMR 2 dimension (HMQC, COSY, HMBC and DEPT-135) spectroscopic as 2,6,4'-trihydroxy-4-methoxybenzophenon. This compound as well as the ethyl acetate extract showed antioxidant activity on DPPH with IC50 was 10.57 and 101.06 μg/mL, respectively. This compound showed strong antioxidant activity on DPPH, almost to the standard antioxidant activity of quercetin (IC50 of 2.93 μg/mL)

scholarly journals Isolation and Characterization of Lupeol from the Stem of Tapinanthus globiferus (A Rich.) and its Antimicrobial Assay

2020 ◽  
Vol 24 (6) ◽  
pp. 1015-1020
Author(s):  
V. Emaikwu ◽  
I.G. Ndukwe ◽  
R. Mohammed ◽  
O.R.A. Iyun ◽  
J.V. Anyam
Keyword(s):  
Spectral Analysis ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Crude Extract ◽  
Solvent System ◽  
Pentacyclic Triterpenoid ◽  
Isolation And Characterization ◽  
Reported Data ◽  
Antimicrobial Assay

Lupeol, a pentacyclic triterpenoid, was isolated from hexane and ethyl acetate solvent system. In antiquity, the stem and leaf infusion of Tapinanthus globiferus has been used ethno-medicinally as a remedy for stomach ache, diarrhea, dysentery, and wounds. Lupeol isolation from this species was carried out by column chromatography after concentrating the crude extract using a rotary evaporator, and the structure was determined by analysis of the isolate by IR, 13CNMR, 1HNMR, HSQC, and HMBC spectral analysis as well as comparison with reported data. This is the first isolation of lupeol from the stem of this species. Keywords: Tapinanthus globiferus, Column chromatography, dysentery, Lupeol

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