scholarly journals ANTI DIABETIC FLAVANONE COMPOUND FROM THE LEAVES OF Artocarpus communis

2010 ◽  
Vol 9 (3) ◽  
pp. 505-508 ◽  
Author(s):  
Puspa D.N. Lotulung ◽  
Sofa Fajriah ◽  
Andini Sundowo ◽  
Euis Filaila
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Inhibition Assay ◽  
Spectroscopic Evidence

The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Artocarpus communis leaves using column chromatography techniques. The structure of the flavanone compound was elucidated on the basic of spectroscopic evidence and comparison to published values. This compound, 8-geranyl-4,5,7-trihydroxyflavone, showed strong anti diabetic activity on α-glucosidase inhibition assay with IC50 18.120 µg mL-1.   Keywords: Artocarpus communis, 8-geranyl-4,5,7-trihydroxyflavone, anti diabetic activity

scholarly journals Isolation and Elucidation Structure of 28-Hydroxy-3-Friedelanone of Nyamplung (Callophyllum inophyllum, Linn.) Leaves

2020 ◽  
Vol 9 (2) ◽  
pp. 117-125
Author(s):  
Triana Kusumaningsih ◽  
◽  
Muhammad Widyo Wartono ◽  
Nais Puji Wijanarti ◽  
◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Nonpolar Solvent ◽  
Ether Extract ◽  
Diethyl Ether Extract ◽  
Isolated Compound

The isolation triterpenoid from Nyamplung (Callophyllum inophyllum, Linn.) leaves has been conducted. The isolation was employed by maceration using ethanol as solvent and liquids extraction using ethyl acetate. Ethyl acetate extract was partitioned successively using nonpolar solvent with hexane, dichloromethane, and diethyl ether, respectively. The diethyl ether extract was purified by column chromatography. The isolated compound of fraction D1 was obtained as white solids crystal with yield of 0.0035%. The isolated compound was determined based on the FTIR, 1HNMR, 13CNMR, HSQC, and HMBC spectra. The isolated compound was identified as 28-hydroxy-3-friedelanone.

Biomedical applications of fish gut associated Streptomyces maritimus IM20 isolated from Rastrelliger kanagurta (Indian mackerel)

2021 ◽  
Vol 16 (10) ◽  
pp. 120-130
Author(s):  
Angamuthu Vignesh ◽  
Venugopal Gopikrishnan ◽  
Sivaraj Anbarasu ◽  
Manikkam Radhakrishnan ◽  
Joseph Jerrine
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Bioactive Metabolites ◽  
Molecular Characteristics ◽  
Inhibition Assay ◽  
Indian Mackerel ◽  
Fish Gut ◽  
Rastrelliger Kanagurta

The biomedical potential of fish gut-associated actinobacteria isolated from the marine fish Rastrelliger kanagurta (Indian mackerel) was investigated. The actinobacterial strain IM20 was isolated from the fish gut by using Kusters agar medium prepared with 50% sea water. Based on their phenotypic and molecular characteristics, strain IM20 was identified as Streptomyces maritimus. The bioactive metabolites produced from the strain IM20 by agar surface fermentation and ethyl acetate extraction were tested for in vitro antimicrobial, antiquorum sensing , anti-biofilm, anti TB, anti-oxidant and anti-cancer activity. The MIC value of ethyl acetate extract (EAE) of IM20 was found to be 16 μg ml−1 against S. aureus and E. coli. In quorum sensing inhibition assay, the extract showed violacein inhibition upto 87% at 512 μg ml−1 concentration when tested by pigment inhibition assay using C. violaceum MTCC 2656. The results of in vitro assays revealed that the ethyl acetate extract of IM-20 (EAE-IM20) showed 75%, 83% and 72% inhibition against Mycobacterium tuberculosis H37Rv, M. tuberculosis (SHRE sensitive) and multi drug resistant (MDR) M tuberculosis respectively at 500 μg ml−1 concentration. In DPPH assay, 71% radical scavenging activity was exhibited by the EAE-IM20 at 250 μg ml−1. In MTT assay, EAEIM20 exhibited 74.29±2.01% inhibition on breast cancer cell line MCF7 (250 μg ml−1). This study broadly determines that the fish associated actinobacteria is a prolific place for diverse multifunctional bioactive compounds for the development of medically important unique drugs.

scholarly journals ANTIOXIDANT ACTIVITY OF 2,6,4’-TRIHYDROXY-4-METHOXY BENZOPHENONE FROM ETHYL ACETATE EXTRACT OF LEAVES OF MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)

2011 ◽  
Vol 11 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Susilawati Susilawati ◽  
Sabirin Matsjeh ◽  
Harno Dwi Pranowo ◽  
Chairil Anwar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Chemical Constituent ◽  
Traditional Medicines ◽  
Phaleria Macrocarpa ◽  
Spherical Crystal

Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is included into family of Thymelaeaceae is one of Indonesia's traditional medicines. Chemical constituent has been isolated from ethyl acetate extract of leaves of mahkota dewa. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The ethyl acetate extract was separated and fractionated by column chromatography. The first fraction was purified by TLC preparative and recrystalization. Compound was isolated as red-brown spherical crystal in 8 mg (m.p. 129-131 °C). Its spot gave dark fluoroscence at TLC plate (UV366) with Rf of 0.3 at TLC chromatogram with eluent of n-hexane : ethyl acetate (7:3); 0.6 with n-hexane : ethyl acetate (1:1); 0.9 with -hexane : ethyl acetate (4:6). This compound was dissolved in methanol. Compound was identified by UV, IR, 1H NMR, 13C NMR and NMR 2 dimension (HMQC, COSY, HMBC and DEPT-135) spectroscopic as 2,6,4'-trihydroxy-4-methoxybenzophenon. This compound as well as the ethyl acetate extract showed antioxidant activity on DPPH with IC50 was 10.57 and 101.06 μg/mL, respectively. This compound showed strong antioxidant activity on DPPH, almost to the standard antioxidant activity of quercetin (IC50 of 2.93 μg/mL)

scholarly journals ISOLASI KUMARIN DARI KULIT BUAH LIMAU SUNDAI (Citrus nobilis Lour)

2017 ◽  
Vol 18 (02) ◽  
pp. 137-145
Author(s):  
Melindra Mulia
Keyword(s):  
Melting Point ◽  
Ir Spectra ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
White Needle ◽  
Pure White

Coumarin has been isolated and characteritated from rind of  Citrus nobilis Lour by maseration methode with methanol. After fractionation by n-hexane and ethyl acetate, collected the phase ethyl acetate which positive of coumarin. From ethyl acetate extract coumarin have been isolated by column chromatography. The isolation results was obtain 2,159 g of pure white needle-shape crystalline with the melting point of 126,2-127,60C. Structure of the isolated coumarine was elucidated by spectroscopic methodes, UV-Vis,13C-NMR, 1H-NMR, 2D-NMR (DEPT/HSQC, COSY, NOESY, HMBC) and IR spectra. Based on the spectra data the isolated coumarine is marmin.

scholarly journals Effect of extraction solvents on the phytochemical content and bioactivity of Momordica charantia Linn. fruits

2021 ◽  
Vol 17 (1) ◽  
pp. 79-83
Author(s):  
Nurul Dalila Abdul Rahim ◽  
Harisun Yaakob ◽  
Rosnani Hasham @ Hisam ◽  
Mohamad Roji Sarmidi ◽  
Kian-Kai Cheng
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Momordica Charantia ◽  
The Other ◽  
Inhibition Assay ◽  
Total Saponin ◽  
Obesity And Diabetes ◽  
Extraction Solvents

Momordica charantia (M. charantia) is a herbaceous climber commonly found in Southeast Asia with therapeutic importance for various illnesses. This study focused on the effect of extraction solvents on saponins-containing compounds from M. charantia and their bioactivities. Different organic solvents including water, ethanol, ethyl acetate, a mixture of methanol-water and methanol-n-butanol were used in the extraction process. The total saponin content, total flavonoid and phenolic content for each extract were examined. In addition, the antioxidant capacity of these extracts were evaluated using both 1,1-diphenyl-2-picrylhydrazyl (DPPH) Free Radical Scavenging Activity and 2,2’-azino-bis-3- ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. Furthermore, a-amylase and lipase inhibition assay were carried out using an in vitro model. The result showed that methanol-n-butanol extracts exhibited the highest total saponin, flavonoid, phenolic content, and ABTS antioxidant activity compared to the other extracts. The a-amylase inhibition assay revealed that water extract and methanol-n-butanol extract from M. charantia contained potent a-amylase inhibitor. On the other hand, the ethyl acetate extract was found to have the most antioxidant capacities based on DPPH radical scavenging assay. The ethyl acetate extract also exhibited the highest inhibition of lipase activities. In conclusion, the methanol-n-butanol solvent was found to be the most effective in extracting saponin from M. charantia. The M. charantia extracts showed inhibition of a-amylase and lipase activities which may suggest the therapeutic potential of M. charantia for obesity and diabetes.

scholarly journals EVALUATION OF ANTIOXIDANT, Α-GLUCOSIDASE AND Α-AMYLASE INHIBITORY ACTIVITIES OF ATALANTIA RACEMOSA AND SENNA UNIFLORA LEAVES

2018 ◽  
Vol 11 (5) ◽  
pp. 254
Author(s):  
Adhav Rahul ◽  
Deokule Subhash
Keyword(s):  
Ethyl Acetate ◽  
Sulfonic Acid ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Inhibition Assay ◽  
Flavonoid Content ◽  
Inhibitory Activities

 Objective: The current investigation was conducted to investigate the total phenol, total flavonoid content, antioxidant, α-glucosidase, and α-amylase activities in leaves of Atalantia racemosa and Senna uniflora. Methods: Different crude solvent extracts were prepared and concentrated using rotary evaporator, these solvent extracts were tested to estimate the antioxidant radical scavenging activity using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2, 2’-azinobis, 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods using spectrophotometer. The extracts were screened by α-amylase inhibition assay and α-glucosidase inhibition assay to estimate the antidiabetic potential of the studied plant with the help of microplate reader.Results: A potent antioxidant activity, i.e. mean percentage inhibition of DPPH and ABTS radical was observed in A. racemosa ethanol (71.5 ± 0.026%) and ethyl acetate extract (97.3 ± 0.076%), respectively, at the concentration of 400 μg/mL. Similarly, highest α-amylase and α-glucosidase inhibitory activities were observed in A. racemosa methanolic (82.4 ± 0.016%) and ethyl acetate 91.1±0.018 extracts, respectively.Conclusions: The present study revealed that the A. racemosa extracts possessed good antioxidant, α-amylase and α-glucosidase inhibitory activities; hence, it can be used as a source of natural free radical scavenger and antidiabetic supplement(s). However, further study needs to be carried out to know the active compound and its mode of action.

scholarly journals STUDY ON CHEMICAL CONSTITUENT AND BIOACTIVITIES OF THE FRUITS OF DIPTEROCARPUS RETUSUS BLUME. DIPTEROCARPACEAE OF VIETNAM

2019 ◽  
Vol 57 (3) ◽  
pp. 294
Author(s):  
Ho Dac Hung ◽  
Doan Huy Tien ◽  
Nguyen Thi Ngoan ◽  
Ba Thi Duong ◽  
Do Quoc Viet ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Chemical Constituent ◽  
Cytotoxic Activities ◽  
Spectroscopic Methods ◽  
Trans Stilbene

The first study on chemical constituents and biological activities of fruits of Dipterocarpus retusus Blume (Dipterocarpaceae.) growing in Vietnam was reported. Column chromatography of the ethyl acetate extract led to the isolation of five compounds: eleutherol (1), 3, 5, 4' - trihydroxy - trans - stilbene (2), polydatin (3) and β-sitosterol (4), β-sitosterol-3-O-β-D-glucopyranoside (5). Their structures were elucidated based on the NMR spectroscopic methods and comparison with literature date. The extracts from fruits of D. retusus Blume by n-hexane, ethyl acetate and butanol, consecutively, were evaluated for antioxidative and cytotoxic activities.

scholarly journals Antimalarial Activity of Sea Sponge Extract of Stylissa massa originating from waters of Rote Island

2021 ◽  
Vol 24 (4) ◽  
pp. 136-145
Author(s):  
Jefry Presson ◽  
Respati Tri Swasono ◽  
Sabirin Matsjeh ◽  
Meta Permata Putri ◽  
Zulfah Az Zahra ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Ethyl Acetate Fraction ◽  
Solvent Mixture ◽  
Extraction Yield ◽  
Unknown Compound ◽  
System Method

Research on the isolation, toxicity test, antimalarial test, and identification of the active compound from the ethyl acetate fraction of Stylissa massa sponge from Oenggae waters, Rote Island, has been conducted. This study aimed to investigate the antimalarial activity of the ethyl acetate fraction of the Stylissa massa sponge. Isolation was carried out by the extraction method using a mixed solvent of methanol: dichloromethane of 3: 2 (v/v), then the extract was partitioned in a solvent mixture of ethyl acetate: water of 1: 2 (v/v). The ethyl acetate extract obtained was separated by column chromatography using the gradient polarity system method. The toxicity test of each fraction was carried out by the Brine Shrimp Lethality Test (BSLT) method, and the antimalarial test was carried out by the haematin polymerization inhibition method. Identification of compounds from the active fraction in the antimalarial test was carried out using Liquid Chromatography-Mass Spectrometry (LC-MS). The extraction yield was 1.14 g (0.23%) of the ethyl acetate extract in the form of a dark brownish-yellow oily solid. Separation by column chromatography resulted in 15 fractions. Toxicity test results showed the four most active fractions with LC50 values, which are very promising for new drug discovery. The IC50 value in the antimalarial activity test of the four fractions indicated that the Stylissa massa sponge ethyl acetate extract was more active than the standard chloroquine compound (115 μg/mL). The LC-MS analysis indicates that fraction 11 contains two compounds that have been reported, and 1 compound is unknown. In contrast, fraction 14 indicates that it contains three compounds that have been reported and one unknown compound.

scholarly journals Senyawa Fenolik dari Daun Tanaman Kalanchoe prolifera (Crassulaceae)

2017 ◽  
Vol 3 (1) ◽  
pp. 27-34
Author(s):  
Yenny Febriani Yun ◽  
Lilis Siti Aisyah ◽  
Tri Reksa Saputra ◽  
Arif Rahman Hakim ◽  
Sari Purbaya ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Spectroscopic Evidence ◽  
Methyl Caffeate

Phenolic compounds such as kaempferol (1), quercetin (2), and methyl caffeate (3) have been isolated from the ethyl acetate extract of Kalanchoe prolifera (Crassulaceae). The chemical structure of isolated compounds 1-3 were elucidated on the basis of spectroscopic evidence (UV, IR, NMR) and comparison with those compound previouly reported.DOI: http://dx.doi.org/10.15408/jkv.v0i0.5037 

scholarly journals Antioxidant Activity of Daemonorops draco Resin

2019 ◽  
Vol 22 (5) ◽  
pp. 179-183 ◽  
Author(s):  
Sri Purwanti ◽  
Wulan Tri Wahyuni ◽  
Irmanida Batubara
Keyword(s):  
Antioxidant Activity ◽  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Layer Chromatography

Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

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