scholarly journals Isolation and characterization of antimicrobial compounds from Cotinus coggygria Scop. ethyl acetate extract

2020 ◽  
Vol 50 (3) ◽  
Author(s):  
Ali Şen ◽  
Ayşe Seher Birteksöz Tan ◽  
Şükran Kültür ◽  
Leyla Bitiş
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Compounds ◽  
Isolation And Characterization ◽  
Cotinus Coggygria

scholarly journals ISOLATION AND CHARACTERISATION OF BERGENIN FROM ETHYL ACETATE EXTRACT OF FLUEGGEA VIROSA LEAVES

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
C. T. Agber ◽  
T. A. Tor-Anyii ◽  
J. O. Igoli ◽  
J. V. Anyam
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Amorphous Solid ◽  
Isocratic Elution ◽  
Tropical Plant ◽  
Isolation And Characterization ◽  
Accessible Method ◽  
Vacuum Liquid Chromatography ◽  
C Nmr

Bergenin is an important constituent of Flueggea virosa (Euphorbiaceae), a tropical plant with several traditional uses. While there are numerous reports on the isolation and characterization of bergenin, a rapid, high-throughput, readily accessible method for the isolation and characterization of the compound locally has not been reported. Isocratic elution of ethyl acetate extract via vacuum liquid chromatography (VLC) with methanol produced a white amorphous solid (100 mg), which was successfully isolated from 250 g of the plant. On the basis of spectral data (1H, C NMR, COSY, HSQC and HMBC) and comparison with literature reports, the structure of this solid was shown to be bergenin, a dihydroisocoumarin derivative of glucopyranosyl gallic acid.

scholarly journals ISOLASI DAN KARAKTERISASI EKSTRAK ETANOL DAUN LEILEM (Clerodendrum minahassae Teijsm dan Binn.) DENGAN METODE SPEKTROFOTOMETRI ISOLATION AND CHARACTERIZATION ETHANOL EXTRACT OF LEILEM LEAVES (Clerodendrum minahassae Teijsm and Binn) WITH METHODS SPECTROPHOTOMETRIC

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Yuri Pratiwi Utami ◽  
Imrawati Imrawati ◽  
Abd Rasyid
Keyword(s):  
Positive Result ◽  
Ethyl Acetate ◽  
Bacterial Cell ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Isolation And Characterization ◽  
Ft Ir

ISOLASI DAN KARAKTERISASI EKSTRAK ETANOL DAUN LEILEM (Clerodendrum minahassae Teijsm dan Binn.) DENGAN METODE SPEKTROFOTOMETRIISOLATION AND CHARACTERIZATION ETHANOL EXTRACT OF LEILEM LEAVES (Clerodendrum minahassae Teijsm and Binn) WITH METHODS SPECTROPHOTOMETRICYuri Pratiwi Utami1), Imrawati1), Abd.Rasyid.D1)1)Program studi sarjana farmasi, Sekolah Tinggi Ilmu Farmasi MakassarJl. Perintis Kemerdekaan KM.13,7 Daya telp/fax. 0411-583190 Makassar [email protected] leaf (Clerodendrum minahassae Teijsm .And Binn.) have been reported in traditional medicine for the treatment of various deseases such as stomatch ache and ascariasis. In Minahassa, leilem leaf generally consumed as vegetables. The present study revealed that leilem leaf can inhibit the growth of escherhia coli because of the presence of flavonoid that can demage bacterial cell membranes. This study at characterization of isolated compounds from ethanol extract of leilem leaves (Clerodendrum minahassae Teijsm and Binn). Extraction was done maceration using 70% ethanol with the percentage of rendement was 10,19%. The phytochemical analysis of ethanol extract of leilem leaves showed a positive result of alkoloid, flavonoid, streroid and tanin. The ethyl acetate extract was selected and 2 grams of it was taken to continued to second conventional column chromatography and 5 fractions were obtained. Fraction 2 was choosen and continued to preparative and result 2 isolates. Isolate 2 that showed one spot on tlc with rf 0,66. Was continued for spectrofotometri uv-vis and ftir analysis. The spectrophotometer uv-vis maximum wavalength at 269,0 nm and 333,0 nm. The ftir data indicated the presence of functional O-H, C-H, C=C and C-O which is suspected ad flavonoid.Keywords : Isolation, Characterization, leilem leaf (Clerodendrum minahassae Teijsm and Binn)ABSTRAKDaun leilem (Clerodendrum minahassae Teijsm dan Binn.) dilaporkan yaitu sebagai obat tradisional untuk menyembuhkan beberapa penyakit, seperti sakit perut dan Ascariasis. Daun leilem biasanya dikonsumsi sebagai sayuran oleh masyarakat di Minahasa. Penelitian ini bertujuan untuk mengetahui profil (karakter) senyawa yang diisolasi dari ekstrak etanol daun leilem (Clerodendrum minahassae Teijsm dan Binn.) ekstrak diperoleh dari proses maserasi menggunakan pelarut etanol 70% dengan persen rendamen 10,19%. Hasil uji kandungan kimia ekstrak etanol daun leilem positif mengandung alkaloid, flavonoid, steroid dan tanin. Ekstrak etil astetat dipilih dan diambil 2 gram dilanjutkan untuk dilakukan pemisahan menggunakan komatografi kolom konvensional dan menghasilkan 5 fraksi. Fraksi 3 dipilih dilanjutkan kromatografi kolom konvensional kedua dan didapatkan 5 fraksi. Fraksi 2 dipilih dan dilanjutkan ketahapan kromatgrafi lapis tipis preparatif dan menghasilkan 2 isolat. Isolat 2 yang menunjukkan 1 noda tunggal pada profil KLT dengan niliai Rf 0,66 dilanjutkan untuk dianalisis dengan spektofometri UV-Vis dan FT-IR. Hasil analisis spektrofotometri UV-Vis isolat 2 mempunyai serapan maksimal pada panjang gelombang 269,0 nm dan 333,0 nm-1. Data FT-IR menunjukkan adanya gugus fungsi O-H, C-H, C=C dan C-O yang diduga merupakan senyawa flavonoid. Kata kunci: Isolasi, karakteriksasi, Daun Leilem(Clerodendrum minahassae Teijsm and Binn). 

scholarly journals CHEMICAL CONSTITUENTS FROM ETHYL ACETATE EXTRACT OF SCOPARIA DULCIS LINN.

2018 ◽  
Vol 54 (2C) ◽  
pp. 278
Author(s):  
Ton Nu Lien Huong
Keyword(s):  
Ethyl Acetate ◽  
Spectral Data ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Natural Compounds ◽  
Oroxylin A ◽  
Scoparia Dulcis ◽  
Isolation And Characterization ◽  
Nitrogen Containing Compounds

This research focuses on the isolation and characterization of eight natural compounds fromthe ethyl acetate extract of Scoparia dulcis L. collected in An Giang province, Vietnam. Sixflavonoid compounds: apigenin (1), luteolin (2), baicalein (3), oroxylin A (4), oroxylin A 7-O-β-D-glucoside (5) and vitexin (6) along with two nitrogen-containing compounds: 2,4-dihydroxy-1,4-benzoxazin-3-one (7) and uracil (8) were successfully isolated via the methods of naturalproduct extraction. The structures of these compounds were elucidated base on 1D-NMR and2D-NMR spectral data as well as MS and compared with those in the authentic reports. Thisstudy contributed new results to the phytochemical examination of Scoparia dulcis Linn.growing in Vietnam with the presence of the first isolated compounds as (3), (4), (5), (7) and (8).

scholarly journals Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior)

2017 ◽  
Vol 3 (1) ◽  
pp. 79-87
Author(s):  
Alfindah Rusanti ◽  
Dede Sukandar ◽  
Tarso Rudiana ◽  
Adawiah Adawiah
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Mtt Assay ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Cytotoxic Compounds ◽  
Murine Leukemia ◽  
Etlingera Elatior

The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’-dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone.DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640

scholarly journals BIOLOGICAL CONTROL OF PHYTOPATHOGENIC FUNGI USING DIFFERENT EXTRACTS OF CHAETOMIUM CUPREUM

2018 ◽  
Vol 11 (9) ◽  
pp. 328
Author(s):  
Sharmila Tirumale ◽  
Nazir Wani
Keyword(s):  
Ethyl Acetate ◽  
Mycelial Growth ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Purification And Characterization ◽  
Butanol Extract ◽  
Ethyl Acetate Extracts

Objective: This study evaluated the Chaetomium cupreum extracts as biocontrol agents against four plant pathogenic fungi (Cladosporium cladosporioides, Fusarium oxysporum, Phomopsis azadirachtae, and Rhizoctonia solani).Method: The antifungal activity of n-butanol and ethyl acetate extracts of C. cupreum was evaluated against plant pathogenic fungi using food poison method.Result: In n-butanol extract, the percentage inhibition of mycelial growth against C. cladosporoides was 88.3±0.1, F. oxysporum was 59.4±0.2, R. solani was 56.2±0.9, and P. azadirachtae was 52.0±0.1at 0.25 mg/ml, respectively. In ethyl acetate extract, the percentage inhibition of mycelial growth against C. cladosporoides was 86.0±0.5, F. oxysporum was 66.4±0.1, P. azadirachtae was 55.2±0.9, and R. solani was 52.0±0.1 at 0.25 mg/ml, respectively.Conclusion: It was found that n-butanol extract is more effective than ethyl acetate extract of C. cupreum. Future studies will focus on the purification and characterization of compounds of C. cupreum and their biocontrol capacity with the mechanism for plant pathological applications.

scholarly journals Isolation and Characterization of Lupeol from the Stem of Tapinanthus globiferus (A Rich.) and its Antimicrobial Assay

2020 ◽  
Vol 24 (6) ◽  
pp. 1015-1020
Author(s):  
V. Emaikwu ◽  
I.G. Ndukwe ◽  
R. Mohammed ◽  
O.R.A. Iyun ◽  
J.V. Anyam
Keyword(s):  
Spectral Analysis ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Crude Extract ◽  
Solvent System ◽  
Pentacyclic Triterpenoid ◽  
Isolation And Characterization ◽  
Reported Data ◽  
Antimicrobial Assay

Lupeol, a pentacyclic triterpenoid, was isolated from hexane and ethyl acetate solvent system. In antiquity, the stem and leaf infusion of Tapinanthus globiferus has been used ethno-medicinally as a remedy for stomach ache, diarrhea, dysentery, and wounds. Lupeol isolation from this species was carried out by column chromatography after concentrating the crude extract using a rotary evaporator, and the structure was determined by analysis of the isolate by IR, 13CNMR, 1HNMR, HSQC, and HMBC spectral analysis as well as comparison with reported data. This is the first isolation of lupeol from the stem of this species. Keywords: Tapinanthus globiferus, Column chromatography, dysentery, Lupeol

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

Antifungal Activity and Molecular Docking of Phenol, 2, 4-Bis (1, 1-Dimethylethyl) - Produced By Plant Growth-Promoting Mangrove Actinobacteria; Kutzneria Sp. Strain TSII

2021 ◽  
Author(s):  
Thangarasu Suganya Devi ◽  
Karuppiah Vijay ◽  
Vidyavathi RM ◽  
Ponnuchamy Kumar ◽  
Muthusamy Govarthanan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Ethyl Acetate ◽  
In Silico ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Mangrove Sediment ◽  
Antimicrobial Compounds ◽  
Antifungal Metabolites ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract The present study reveals the plant growth-promoting (PGP) potentials and characterizes the antifungal metabolites of Kutzneria sp. strain TSII isolated from mangrove sediment soil through in vitro and in silico studies. In this study, Kutzneria sp. strain TSII was screened for PGP activities and the antifungal activities against Pithomyces atro-olivaceous, a leaf spot associated pathogen in groundnut plants. The ethyl acetate extract of Kutzneria sp. strain TSII was purified using column chromatography, and the presence of various antimicrobial compounds was studied by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. In silico modeling and docking were carried out to evaluate the antifungal potent of bioactive compound. Kutzneria sp. strain TSII produced Proteases, Phosphatases, Ammonia, Siderophores, Cellulases, Indole Acetic Acid (IAA), Lipases, and Amylases, indicating its ability to enhance the growth of plants. The ethyl acetate extract of Kutzneria sp strain TSII was found to be a potent inhibitor of fungal mycelial growth in the potato dextrose agar (PDA) plates. The GC-MS spectral study showed 24 antimicrobial compounds belonging to five chemical groups: Phenolics, Phthalates, Fatty acid methyl esters (FAME), Spiro, and Fatty alcohols. In silico docking studies showed that Phenol, 2, 4-Bis (1, 1-Dimethylethyl) – effectively attaches with the active site of mitochondrial F1F0 Adenosine triphosphate synthase enzymes of Pithomyces atro-olivaceous. Hence, it is clear that these antifungal compounds shall be formulated shortly to treat many plant fungal diseases in an eco-friendly manner.

Sign in / Sign up

Export Citation Format

Share Document