Photodynamic Therapy Using Endoscopy Capsule Robot

Among the photosensitizers used in Photodynamic therapy (PDT) technique for cancer treatment, it is found out that the Methylene blue and glycoconjugates chlorine are the best ones for this purpose. In this paper, it is suggested to use Active Capsule Wireless Endoscopy Robot instead of the traditional endoscope. The capsule has many valuable features. It uses LEDs as a source of light in the PDT to kill the colon cancer cells. So, the doctor can make use of the advantage of applying the LED light locally at the tumor which was previously injected by the photosensitizers, the light activates these photosensitizers and a photochemical reaction starts that makes the colon cancer cells die. The light with effective wavelength and power density, energy level and controlled LED light intensity will be applied. Active locomotion capsule endoscopy with an electromagnetic actuation system that can achieve a 3-D locomotion and guidance within the digestive system. The paper also discussed how to manage the required power in the capsule for all parts, LEDs, camera, transceiver, and locomotion.

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Photodynamic Therapy at Low-Light Fluence Rate: in vitro Assays on Colon Cancer Cells

IEEE Journal of Selected Topics in Quantum Electronics ◽

10.1109/jstqe.2018.2889426 ◽

2019 ◽

Vol 25 (1) ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

J. A. Rodrigues ◽

R. Amorim ◽

M. F. Silva ◽

F. Baltazar ◽

R. F. Wolffenbuttel ◽

...

Keyword(s):

Colon Cancer ◽

Photodynamic Therapy ◽

Cancer Cells ◽

Fluence Rate ◽

Colon Cancer Cells ◽

Low Light ◽

Light Fluence

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Effective Photodynamic Therapy for Colon Cancer Cells Using Chlorin e6 Coated Hyaluronic Acid-Based Carbon Nanotubes

International Journal of Molecular Sciences ◽

10.3390/ijms21134745 ◽

2020 ◽

Vol 21 (13) ◽

pp. 4745 ◽

Cited By ~ 2

Author(s):

Prabhavathi Sundaram ◽

Heidi Abrahamse

Keyword(s):

Carbon Nanotubes ◽

Colon Cancer ◽

Cell Death ◽

Photodynamic Therapy ◽

Hyaluronic Acid ◽

Cancer Cells ◽

Chlorin E6 ◽

Therapeutic Modality ◽

Size Analysis ◽

Colon Cancer Cells

Colon cancer is the third major cancer contributor to mortality worldwide. Nanosized particles have attracted attention due to their possible contribution towards cancer treatment and diagnosis. Photodynamic therapy (PDT) is a cancer therapeutic modality that involves a light source, a photosensitizer and reactive oxygen species. Carbon nanotubes are fascinating nanocarriers for drug delivery, cancer diagnosis and numerous potential applications due to their unique physicochemical properties. In this study, single walled carbon nanotubes (SWCNTs) were coupled with hyaluronic acid (HA) and chlorin e6 (Ce6) coated on the walls of SWCNTs. The newly synthesized nanobiocomposite was characterized using ultraviolet-visible spectroscopy, Fourier transform electron microscopy (FTIR), X-ray diffraction analysis (XRD), particle size analysis and zeta potential. The loading efficiency of the SWCNTs-HA for Ce6 was calculated. The toxicity of the nanobiocomposite was tested on colon cancer cells using PDT at a fluence of 5 J/cm2 and 10 J/cm2. After 24 h, cellular changes were observed via microscopy, LDH cytotoxicity assay and cell death induction using annexin propidium iodide. The results showed that the newly synthesized nanobiocomposite enhanced the ability of PDT to be a photosensitizer carrier and induced cell death in colon cancer cells.

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The influence of hypericin-mediated photodynamic therapy on secretion of selected interleukins by colon cancer cells in vitro

17th International Photodynamic Association World Congress ◽

10.1117/12.2525516 ◽

2019 ◽

Author(s):

Aleksandra Kawczyk-Krupka ◽

Marta Kaleta-Richter ◽

Anna Miedzybrodzka ◽

Wojciech Latos ◽

Aleksander Sieron ◽

...

Keyword(s):

Colon Cancer ◽

Photodynamic Therapy ◽

Cancer Cells ◽

Colon Cancer Cells

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The Influence of Hypericin-Mediated Photodynamic Therapy on Interleukin-8 and -10 Secretion in Colon Cancer Cells

Integrative Cancer Therapies ◽

10.1177/1534735420918931 ◽

2020 ◽

Vol 19 ◽

pp. 153473542091893 ◽

Cited By ~ 1

Author(s):

Marta Kaleta-Richter ◽

David Aebisher ◽

Dagmara Jaworska ◽

Zenon Czuba ◽

Grzegorz Cieślar ◽

...

Keyword(s):

Colon Cancer ◽

Photodynamic Therapy ◽

Cancer Cells ◽

Cell Line ◽

Sw480 Cell ◽

Experimental Models ◽

Colon Cancer Cells ◽

Sw620 Cell ◽

Sublethal Doses

The aim of this study was to measure the secretion of interleukin (IL)-8 and -10 during an elicited immune response following sublethal doses of hypericin-mediated photodynamic therapy (HY-PDT) in experimental models of residual colon cancer cells in vitro. Investigations were performed on the cancer cell lines SW480 and SW620. Each cell line was exposed to 3 different concentrations of the photosensitizer HY and various doses of irradiation. The cell metabolic activity using an MTT assay was performed and then the measurement of IL-8 and IL-10 secretion was achieved using the Bio-Plex ProTMAssay. There was a statistically significant amplification of IL-8 secretion during HY-PDT in the SW620 cell line (at 1 J/cm2: P = .01, 5 J/cm2: P = .002, and 10 J/cm2: P = .025) and a statistically significant decrease in IL-8 during HY-PDT in the SW480 cell line (at 1 J/cm2: P = .05, 5 J/cm2: P = .035, and 10 J/cm2: P = .035). No statistically significant differences in IL-10 concentration were found following HY-PDT in the SW480 (at 1 J/cm2: P > .4, 5 J/cm2: P = .1, and 10 J/cm2: P = .075) or in the SW620 cell line (at 1 J/cm2: P > .4, 5 J/cm2: P > .4, and 10 J/cm2: P > .4). HY-PDT can both eliminate and control a primary tumor via cytotoxic effects, and at sublethal doses, it can affect IL release by colon cancer cells. In this experiment, this influence depended on the level of tumor cell metastatic activity.

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