Total oxidant status and total antioxidant capacity in the saliva of patients with chronic periodontitis

MedPharmRes ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 21-26
Author(s):  
Thuy T. Nguyen ◽  
Duc A. Vo ◽  
Truc Thanh Thai ◽  
Thuy L. Vo ◽  
Nam C-N. Huynh
Keyword(s):  
Antioxidant Capacity ◽  
Chronic Periodontitis ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Total Oxidant Status ◽  
Oxidative Stress Biomarkers ◽  
Clinical Attachment Loss ◽  
Attachment Loss ◽  
Total Antioxidant ◽  
Oxidant Status

Background: Recently, methods of quantifying total oxidant status (TOS) and total antioxidant capacity (TAOC) were developed to investigate periodontitis. This study was performed to evaluate the salivary TOS and TAOC levels of patients with chronic periodontitis (CP) and investigating the association between periodontal clinical parameters and these oxidative stress biomarkers. Material and methods: 40 participants (23-65 years old) were classified into two groups of 20 each, namely the CP group (participants with CP) and the control group (periodontally healthy controls). Clinical periodontal parameters were monitored, and TOS and TAOC levels were measured using laboratory assays. Results: TOS level increased in the saliva of patients with CP and the salivary TAOC in patients with CP was significantly lower than that of the control group. The TOS had a positive moderate correlation with the plaque index and clinical attachment loss (r=0.32 and 0.37, respectively) while TAOC was negatively and moderately correlated with clinical attachment loss (r=-0.35). Conclusion: Salivary TOS and TAOC were distinguished in healthy and chronic PD patients. Further studies are required to comprehensively evaluate the potential role of these biomarkers in diagnosis and treatment evaluation of CP.

scholarly journals Niacin improves maturation and cryo-tolerance of bovine in vitro matured oocytes: An experimental study

2019 ◽  
Author(s):  
Mojtaba Kafi ◽  
Mahboobeh Ashrafi ◽  
Mehdi Azari ◽  
Borhan Jandarroodi ◽  
Beheshteh Abouhamzeh ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Embryo Development ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Total Oxidant Status ◽  
Nuclear Maturation ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
Bovine Oocytes

Background: Nicotinic acid (niacin) is a broad-spectrum lipid-modifying agent that has potent antioxidant properties and reduces the production of lipid peroxidation. Objective: The purpose of the present study was to investigate the maturation, embryo development and cryo-tolerance merit, and levels of malondialdehyde (MDA), total oxidant status, and total antioxidant capacity following the supplementation of bovine oocytes maturation medium with different concentrations of niacin. Materials and Methods: Immature cumulus-oocyte complexes were cultured in tissue culture medium-199 maturation media supplemented with 0, 100, 200, and 400 µM niacin under a standard in vitro culture condition. After 24 hr of culture, the nuclear maturation rate was assessed. Then, two groups of immature cumulus-oocyte complexes were cultured in TCM-199 either with or without 400 µM niacin and evaluated for embryo development. Also, matured cumulus-oocyte complexes in both groups were frozen using a standard vitrification procedure. After vitrification, oocytes were warmed in two steps and evaluated for embryo development. In addition, the level of total antioxidant capacity, total oxidant status, and MDA were measured. Results: The results indicated that although the treatment with 400µM niacin increased in vitro nuclear maturation (87.6 ± 5.3), it did not improved the embryo development to the blastocyst stage. Higher cleavage and blastocyst rates were observed in vitrified oocytes that were cultured with supplemented 400 µM niacin compared to the control group (without niacin) (53.6 ± 2.7 and 10.6 ± 1.6 vs. 46.2 ± 4.1 and 6.3 ± 2.4, respectively). Also, the addition of 400 µM niacin to the maturation media could decrease MDA levels after maturation. Conclusion: Niacin could improve the quality of in vitro embryo production (IVP) embryos and tolerance of bovine oocytes to vitrification. Key words: Bovine, Embryonic development, Niacin, Oocytes, Vitrification.

scholarly journals Total Antioxidant Capacity, Catalase Activity and Salivary Oxidative Parameters in Patients with Temporomandibular Disorders

2020 ◽  
Author(s):  
Neda Omidpanah ◽  
Saba Ebrahimi ◽  
Asad Vaisi Raygani ◽  
Hadi Mozafari ◽  
Mansour Rezaei
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Temporomandibular Disorders ◽  
Catalase Activity ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Healthy Controls ◽  
Oxidative Stress Biomarkers ◽  
Reducing Ability ◽  
Total Antioxidant

Objectives: Temporomandibular disorders (TMD) are characterized by pain or discomfort in the temporomandibular joint, periauricular region, masticatory muscles, and neck on one or both sides. It may also be associated with joint sounds, restricted mandibular movements and mandibular deviation. Oxidative agents may have a deleterious role in the pathogenesis of joint diseases, and oxidative stress can lead to TMD. The aim of this study was to assess the oxidative stress biomarkers in the saliva of TMD patients and healthy controls. Materials and Methods: This case-control study was conducted on 30 patients with TMDs (5 males and 25 females) with a mean age of 30.7±13.2 years, and 30 healthy controls (5 males and 25 females) with a mean age of 29.16±11.2 years. Saliva samples were collected according to the standard protocol and the total antioxidant capacity of the saliva (non-enzymatic), catalase activity, and malondialdehyde (MDA) levels were measured using the ferric reducing ability of plasma, Aebi’s method, and high-performance liquid chromatography, respectively. Finally, The MDA levels were analyzed by the Mann-Whitney test. Other quantitative parameters were analyzed by independent t-test.  Results: TMD patients had significantly higher salivary levels of MDA compared to the control group (P=0.001). But there were no significant differences in catalase (P=0.49) and total antioxidant capacity (P=0.22) of TMD patients and healthy controls. Conclusion: It seems that oxidative stress may be involved in the pathogenesis of TMDs.

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