scholarly journals EKSPLORASI JAMUR MAKROSKOPIS DI HUTAN OELUAN KABUPATEN TIMOR TENGAH UTARA

2020 ◽  
Vol 3 (1) ◽  
pp. 4-6
Author(s):  
Lukas Pardosi ◽  
Florian Mayesty Prima Makin ◽  
I Gede Arya Wiguna
Keyword(s):  
Pycnoporus Cinnabarinus ◽  
Polyporus Squamosus

Penelitian mengenai keanekaragaman jamur makroskopis di kawasan Hutan Oeluan kabupaten Timor tengah Utara Belum  pernah dilaporkan. Penelitian ini telah dilaksanakan pada bulan Januari 2020 dengan letak geografis 9° 2¢ 48" LS- 9° 37¢ 36" LS124° 4¢ 2" BT- 124°46¢ 0" BT, penelitian ini dilakukan pada empat jalur yang berbeda. Panjang jalur pengamatan adalah 100 m. Metode yang dilakuan pada setiap Jalur pemgamatan di setiap adalah metode jelajah dan Purpossive sampling, berdasarkan keberadaan jamur makroskopis yang dianggap mewakili kawasan tersebut, setelah itu dilanjutkan dengan mencatat jumlah individu, koleksi dan dokumentasi. Pengamatan dan pengkoleksian spesies dilakukan secara eksploratif. Dari hasil penelitian ditemukan 9 spesies jamur makroskopis yaitu, Ganoderma aplatum, Earliella scabrosa, Polyporus tenuiculus, Polyporus squamosus, Mikroporus xanthopus, Favolus grammocephalus, Pycnoporus cinnabarinus, Ganoderma lucidium, Laccaria vinaceoavellaneae

scholarly journals Keanekaragaman Jamur di Cagar Alam Gunung Mutis Kabupaten Timor Tengah Utara, Nusa Tenggara Timur

2018 ◽  
Vol 3 (2) ◽  
pp. 105
Author(s):  
Hartini Solle ◽  
Ferdinandus Klau ◽  
Simon Taka Nuhamara
Keyword(s):  
Pycnoporus Cinnabarinus ◽  
Auricularia Auricula ◽  
Piptoporus Betulinus ◽  
Coriolus Hirsutus ◽  
Polyporus Squamosus

Jamur merupakan salah satu organisme yang memegang peranan penting dalam menguraikan bahan organik yang sangat kompleks menjadi bahan sederhana sehingga mudah diserap oleh organisme lainnya. Tujuan penelitian untuk mengetahui jenis jamur dan mengetahui tingkat keanekaragaman jenis jamur pada hutan cagar alam gunung Mutis. Metode yang digunakan adalah metode jelajah setiap plot dengan mencatat jenis jamur yang ditemukan pada kawasan tersebut dan dilanjutkan dengan proses identifikasi jenis jamur yang ditemukan. Pengambilan sampel dengan koleksi dan dokumentasi. Hasil penelitian menunjukkan bahwa terdapat 340 individu pada 17 spesies jamur dengan tingkat keanekaragaman : 1,510 yang menunjukkan tingkat keanekaragam spesies jamur yang tinggi. Spesies jamur yang paling mendominasi adalah jamur Microporus sp dan Polyporus sp, sedangkan jenis lain keberadaannya masih tergolong rendah seperti Polyporus squamosus, Coriolus hirsutus, Pycnoporus cinnabarinus, Tyromyces sambuceus, Fomytopsis pinicola, Microporus perula, Trametes orientalis, Piptoporus betulinus, Auricula polytricha, Auricularia auricula, Elfvingia applanata, Fomes sp, Laccaria vinaceoavellaneae, Paxillus curtisii, Pleurotus pulmorius.

DEGRADAÇÃO DA MADEIRA DE EUCALYPTUS SP. POR BASIDIOMICETOS DE PODRIDÃO BRANCA

2007 ◽  
Vol 74 (4) ◽  
pp. 321-328
Author(s):  
L.D. Abreu ◽  
R.H. Marino ◽  
J.B. Mesquita ◽  
G.T. Ribeiro
Keyword(s):  
Pleurotus Ostreatus ◽  
Schizophyllum Commune ◽  
Pycnoporus Cinnabarinus

RESUMO Avaliou-se a degradação de Eucalyptus sp. pelos basidiomicetos Pleurotus ostreatus, Pycnoporus cinnabarinus e Schizophyllum commune, in vitro e em condições de campo. Para tanto, na degradação in vitro foram utilizados discos de Eucalyptus sp. submetidos aos seguintes tratamentos: T1 controle; T2 – 5 mL de água; T3 – 10 mL de água; T4 meio de cultura batata-dextrose-ágar. O parâmetro analisado foi a perda de massa (em %), após 60 e 120 dias de incubação. Em condições de campo foram utilizados corpos de prova deEucalyptus sp. inoculados com substrato “spawn” dePleurotusostreatus, Pycnoporus cinnabarinus e Schizophyllum commune. Foram realizados os seguintes tratamentos: T1 – controle (sem água e sem inóculo); T2 – corpos de prova submersos por 24h em água e T3 – corpos de prova não submersos por 24h em água e avaliada a perda de massa (%), após 60 e 120 dias de incubação. A degradação dos discos de eucalipto in vitro e em condições de campo foi influenciada pelos isolados. Os discos de eucalipto, in vitro, inoculados com Pycnoporus cinnabarinus apresentaram, em média, 25,33% de perda de massa e o micélio foi mais vigoroso em relação ao isolado Pleurotus ostreatus e ao Schizophyllum commune. Os tratamentos empregados e o período de incubação, in vitro, não influenciaram a perda de massa dos discos de eucalipto. Em condições de campo, a perda de massa dos corpos de prova de eucalipto, inoculados com Pycnoporus cinnabarinus, foi de 15,79%, já com Pleurotus ostreatus foi de 12,45% e Schizophyllum commune 12,95%.

scholarly journals Free amino acids in fruit bodies of some wood destroying fungi

2014 ◽  
Vol 14 (1-2) ◽  
pp. 151-155
Author(s):  
Wiesław Tadeusiak ◽  
Eliza Balicka
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Polyporus Squamosus

Concentration of free amino acids in the following bracket fungi: <i>Climacodon septentrionalis</i> (Fr) P. Karst, <i>Hapalopilus croceus</i> (Pers. ex Fr.) Donk., <i>Laetiporusus sulphurens</i> (Bull. ex Fr.) Murill and <i>Polyporus squamosus</i> Huds ex Fr., were determined by ion-exchange chromatography.

scholarly journals Identification of an Oligosaccharide Dehydrogenase from Pycnoporus Cinnabarinus Provides Insights into Fungal Breakdown of Lignocellulose

2021 ◽  
Author(s):  
Gabriele Cerutti ◽  
Elena Gugole ◽  
Linda Celeste Montemiglio ◽  
Annick Turbé-Doan ◽  
Dehbia Chena ◽  
...  
Keyword(s):  
Function Analysis ◽  
Sequence Similarity ◽  
Functional Characterization ◽  
Physiological Role ◽  
Free Form ◽  
Lignocellulose Degradation ◽  
Carbohydrate Active Enzymes ◽  
Recognition Mechanism ◽  
Pycnoporus Cinnabarinus ◽  
Enzymatic Function

Abstract Background: Fungal glucose dehydrogenases (GDHs) are FAD-dependent enzymes belonging to the glucose-methanol-choline oxidoreductase superfamily. These enzymes are classified in the “Auxiliary Activity” family 3 (AA3) of the Carbohydrate-Active enZymes database, and more specifically in subfamily AA3_2, that also includes the closely related flavoenzymes aryl-alcohol oxidase and glucose 1-oxidase. Based on sequence similarity to known fungal GDHs, an AA3_2 enzyme active on glucose was identified in the genome of Pycnoporus cinnabarinus, a model Basidiomycete able to completely degrade lignin.Results: In our work, substrate screening and functional characterization showed an unexpected preferential activity of this enzyme toward oligosaccharides containing a b(1à3) glycosidic bond, with the highest efficiency observed for the disaccharide laminaribiose. Despite its sequence similarity to GDHs, we defined a novel enzymatic activity, namely oligosaccharide dehydrogenase (ODH), for this enzyme. The crystallographic structures of ODH in the sugar-free form and in complex with glucose and laminaribiose unveiled a peculiar saccharide recognition mechanism which is not shared with previously characterized AA3 oxidoreductases and accounts for ODH preferential activity toward oligosaccharides. The sugar molecules in the active site of ODH are mainly stabilized through CH-p interactions with aromatic residues rather than through hydrogen bonds with highly conserved residues, as observed instead for the fungal glucose dehydrogenases and oxidases characterized to date. Finally, three sugar-binding sites were identified on ODH external surface, which were not previously observed and might be of importance in the physiological scenario.Conclusions: Structure-function analysis of ODH is consistent with its role as an auxiliary enzyme in lignocellulose degradation and unveils yet another enzymatic function within the AA3 family of the Carbohydrate-Active enZymes database. Our findings allow deciphering the molecular determinants of substrate binding and provide insight into the physiological role of ODH, opening new perspectives to exploit biodiversity for lignocellulose transformation into fuels and chemicals.

scholarly journals Nueva variedad chilena de pycnoporus cinnabarinus (jacq. Ex.fr.) Karst. Estudio de sus características morfológicas y fisiológicas.

2019 ◽  
Vol 3 (4) ◽  
Author(s):  
Julio A. Burgos ◽  
Sylvia E. Ortiz
Keyword(s):  
Pycnoporus Cinnabarinus

Se describe la variedad ligninolítica Pycnoporus cinnabarinus (Jacq.ex Fr.) Kant. var. osorninus Burgos var. nov. basándonos en que sus características morfológicas difieren claramente de P. cinnabarinus. Además esta variedad tiene una tasa de crecimiento superior a la de P. cinnabarinus. El medio liquido con sulfato de amonio como fuente de nitrógeno estimula la formación de clamidosporas fructificacines en agar-extracto de malta o medios con aserrín de pino forman tubos con 4 poros/mm.

Pretreatment of lignocellulosic material with fungi capable of higher lignin degradation and lower carbohydrate degradation improves substrate acid hydrolysis and the eventual conversion to ethanol

2008 ◽  
Vol 54 (4) ◽  
pp. 305-313 ◽  
Author(s):  
Sarika Kuhar ◽  
Lavanya M. Nair ◽  
Ramesh Chander Kuhad
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Degradation ◽  
Ethanol Yield ◽  
Fungal Isolate ◽  
Fermentation Inhibitors ◽  
Pycnoporus Cinnabarinus ◽  
Acid Hydrolysate ◽  
Carbohydrate Degradation ◽  
Fungal Pretreatment ◽  
Acid Load

Phanerochaete chrysosporium , Pycnoporus cinnabarinus ,and fungal isolates RCK-1 and RCK-3 were tested for their lignin degradation abilities when grown on wheat straw (WS) and Prosopis juliflora (PJ) under solid-state cultivation conditions. Fungal isolate RCK-1 degraded more lignin in WS (12.26% and 22.64%) and PJ (19.30% and 21.97%) and less holocellulose in WS (6.27% and 9.39%) and PJ (3.01% and 4.58%) after 10 and 20 days, respectively, than other fungi tested. Phanerochaete chrysosporium caused higher substrate mass loss and degraded more of holocellulosic content (WS: 55.67%; PJ: 48.89%) than lignin (WS: 18.89%; PJ: 20.20%) after 20 days. The fungal pretreatment of WS and PJ with a high-lignin-degrading and low-holocellulose-degrading fungus (fungal isolate RCK-1) for 10 days resulted in (i) reduction in acid load for hydrolysis of structural polysaccharides (from 3.5% to 2.5% in WS and from 4.5% to 2.5% in PJ), (ii) an increase in the release of fermentable sugars (from 30.27 to 40.82 g·L–1in WS and from 18.18 to 26.00 g·L–1in PJ), and (iii) a reduction in fermentation inhibitors (total phenolics) in acid hydrolysate of WS (from 1.31 to 0.63 g·L–1) and PJ (from 2.05 to 0.80 g·L–1). Ethanol yield and volumetric productivity from RCK-1-treated WS (0.48 g·g–1and 0.54 g·L–1·h–1, respectively) and PJ (0.46 g·g–1and 0.33 g·L–1·h–1, respectively) were higher than untreated WS (0.36 g·g–1and 0.30 g·L–1·h–1, respectively) and untreated PJ (0.42 g·g–1and 0.21 g·L–1·h–1, respectively).

A new genus and two new species of melicharid mites (Acari: Mesostigmata) associated with wood-decaying fungi and mycophagous erotylid beetles (Coleoptera: Erotylidae) in Europe

Zootaxa ◽  
2021 ◽  
Vol 4980 (1) ◽  
pp. 157-173
Author(s):  
PETER MAŠÁN ◽  
OMID JOHARCHI ◽  
VLADIMIR V. ABRAMOV
Keyword(s):  
New Species ◽  
New Genus ◽  
European Part ◽  
Large Spore ◽  
Two New Species ◽  
European Part Of Russia ◽  
Established Genus ◽  
Mite Family ◽  
Polyporus Squamosus

We describe a new genus in the mite family Melicharidae, Mycomelichares Mašán & Joharchi gen. nov., to accommodate two new species, Mycomelichares polypori Mašán & Joharchi sp. nov. and Mycomelichares reductus Mašán & Joharchi sp. nov. on the basis of specimens collected on wood-decaying fungi and/or mycophagous beetles of the genera Triplax Herbst and Tritoma Fabricius (Coleoptera: Erotylidae) in Slovakia and European part of Russia, respectively. Moreover, two previously described Proctolaelaps species—P. cyllodi Samšiňák, 1960 and P. slovacus Mašán, 1998 are transferred to the newly established genus. The new genus is characterized by specific characters in tritosternum (enlarged and brush-shaped, having their laciniae thickened, basaly fused and distally densely pilose), chelicerae (slightly dish-shaped digits with long, narrow and sharp denticles), ventral hypostome (setae h1 enlarged, thickened and distally flattened), setation (tendency towards placement of R series setae on soft integument, and reduction of some setae on idiosoma and legs), and unusual ecological specialisation on fungal substrates. Mycomelichares polypori sp. nov. is adapted to the life in sporophores of the basidiomycete bracket fungus, Polyporus squamosus (Polyporaceae). Mites of this species can be abundantly found on the lower fertile surface of the fungus, including large spore-bearing pores. Furthermore, keys to the melicharid genera reported from Palaearct, and the species of the genus are provided. 

Bioinformatics Analysis of Laccase (Lac1) in Pycnoporus cinnabarinus

2010 ◽  
Vol 121-122 ◽  
pp. 502-506
Author(s):  
He Li ◽  
Guo Ying Zhou ◽  
Huai Yun Zhang ◽  
Liang Guo
Keyword(s):  
Amino Acid ◽  
Manganese Peroxidase ◽  
Bioinformatics Analysis ◽  
Instability Index ◽  
Pycnoporus Cinnabarinus ◽  
Grand Average ◽  
Glycosylation Sites ◽  
The World ◽  
Aliphatic Index

Pycnoporus cinnabarinus is a plant pathogen. It is common in many areas and is widely distributed throughout the world. Laccases of are some of the few oxidoreductases commercialized as industrial catalysts. In the present study, some characters of the amino acid sequence of P.cinnabarinus laccase (Lac1) were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of manganese peroxidase was 4.81 and the theoretical molecular weight of manganese peroxidase was 56292.0 Da; total number of atoms was 7806; the extinction coefficient was 58120 (280 nm). The N-terminal of the sequence considered was M (Met) and the estimated half-life was 30 hours (mammalian reticulocytes, in vitro). The instability index (II) was computed to be 34.50; this classifies the protein as stable. Aliphatic index was 82.64. Grand average of hydropathicity (GRAVY) was -0.063. There were 8 glycosylation sites, a signal peptide and conserved domains.

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