scholarly journals Dissemination of Biomedical Cell Sheets using Reality Technology and Digital Tools

2021 ◽  
Vol 3 (4) ◽  
pp. 46-53
Author(s):  
Gwo-Long Lin ◽  
Peng-Hsin Chen
Keyword(s):  
Cell Therapy ◽  
New Technologies ◽  
Learning Experience ◽  
Cell Sheet ◽  
3D Animation ◽  
Cell Sheets ◽  
The Public ◽  
2D Animation ◽  
Cell Sheet Technology ◽  
Physically Challenged

Nowadays, with the advance of biomedical and pharmaceutical technology, new treatments such as cell therapy, cell sheets, etc. all provide patients other alternatives. However, the public has little knowledge in these new technologies and they also find them difficult to understand. Hence, this paper is aimed at providing the public with state-of-the-art technological knowledge and constructing an innovative field full of diversity, entertainment, and educational meaning with the assistance of Augmented Reality (AR) and Virtual Reality (VR). Taking the cell sheets technology, a kind of cell therapy recently approved in Taiwan, as an example, we built an AR platform, and demonstrated a trailer animation, 2D animation and 3D model animation via Merge Cube. The trailer animation will portrait how the main character helps her friend, who became physically challenged in an accident, stand up again by asking a genius doctor to perform cell sheet technology. The 2D animation will be used to explain how cell sheet works and its application, while the 3D animation helps demonstrate the DNA reproduction and cell division in cell therapy. A VR field will also be set up so that players can play as the genius doctor, fight their way through the VR games, and learn more about cell sheet technology. To let the public learn more about this biotechnology knowledge, we held an exhibition to display the research results, providing them a whole new learning experience.

scholarly journals Designing 3D Mesenchymal Stem Cell Sheets Merging Magnetic and Fluorescent Features: When Cell Sheet Technology Meets Image-Guided Cell Therapy

Theranostics ◽  
2016 ◽  
Vol 6 (5) ◽  
pp. 739-751 ◽  
Author(s):  
Gabriel Rahmi ◽  
Laetitia Pidial ◽  
Amanda K. A. Silva ◽  
Eléonore Blondiaux ◽  
Bertrand Meresse ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Image Guided ◽  
Cell Sheet Technology

scholarly journals Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hallie Thorp ◽  
Kyungsook Kim ◽  
Makoto Kondo ◽  
David W. Grainger ◽  
Teruo Okano
Keyword(s):  
Chondrogenic Differentiation ◽  
Cell Sheet ◽  
Cartilage Regeneration ◽  
Cell Sheets ◽  
Chondrogenic Potential ◽  
Cell Sheet Technology ◽  
Cell Functionality ◽  
Articular Cartilage Regeneration

AbstractCell and tissue engineering approaches for articular cartilage regeneration increasingly focus on mesenchymal stem cells (MSCs) as allogeneic cell sources, based on availability and innate chondrogenic potential. Many MSCs exhibit chondrogenic potential as three-dimensional (3D) cultures (i.e. pellets and seeded biomaterial scaffolds) in vitro; however, these constructs present engraftment, biocompatibility, and cell functionality limitations in vivo. Cell sheet technology maintains cell functionality as scaffold-free constructs while enabling direct cell transplantation from in vitro culture to targeted sites in vivo. The present study aims to develop transplantable hyaline-like cartilage constructs by stimulating MSC chondrogenic differentiation as cell sheets. To achieve this goal, 3D MSC sheets are prepared, exploiting spontaneous post-detachment cell sheet contraction, and chondrogenically induced. Results support 3D MSC sheets’ chondrogenic differentiation to hyaline cartilage in vitro via post-contraction cytoskeletal reorganization and structural transformations. These 3D cell sheets’ initial thickness and cellular densities may also modulate MSC-derived chondrocyte hypertrophy in vitro. Furthermore, chondrogenically differentiated cell sheets adhere directly to cartilage surfaces via retention of adhesion molecules while maintaining the cell sheets’ characteristics. Together, these data support the utility of cell sheet technology for fabricating scaffold-free, hyaline-like cartilage constructs from MSCs for future transplantable articular cartilage regeneration therapies.

scholarly journals Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Mitsuyoshi Nakao ◽  
Kyungsook Kim ◽  
Kenichi Nagase ◽  
David W. Grainger ◽  
Hideko Kanazawa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Sheet ◽  
Cellular Structures ◽  
Phenotypic Traits ◽  
Cell Sheets ◽  
Temperature Responsive ◽  
Cell Sheet Technology ◽  
Responsive Cell

Abstract Background In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inconsistent, largely attributed to transplanted MSC difficulties both in engrafting at tissue sites and in retaining their therapeutic functions from suspension formulations. MSC functional components, including cell adhesion and cell–cell junction proteins, and ECM that contribute to essential cellular therapeutic effects, are damaged or removed by proteolytic enzymes used in stem cell harvesting strategies from culture. To overcome these limitations, methods to harvest and transplant cells without disrupting critical stem cell functions are required. Cell sheet technology, exploiting temperature-responsive cell culture surfaces, permits cell harvest without cell protein damage. This study is focused on phenotypic traits of MSC sheets structurally and functionally to understand therapeutic benefits of cell sheets. Methods/results This study verified cleaved cellular proteins (vinculin, fibronectin, laminin, integrin β-1, and connexin 43) and increased apoptotic cell death produced under standard trypsin harvesting treatment in a time-dependent manner. However, MSC sheets produced without trypsin using only temperature-controlled sheet harvest from culture plastic exhibited intact cellular structures. Also, MSCs harvested using enzymatic treatment (i.e., chemical disruption) showed higher pYAP expression compared to MSC sheets. Conclusion Retention of cellular structures such as ECM, cell–cell junctions, and cell–ECM junctions is correlated with human umbilical cord mesenchymal stem cell (hUC-MSC) survival after detachment from cell culture surfaces. Retaining these proteins intact in MSC cultures using cell sheet technology is proposed to enhance stem cell survival and their function in stem cell-based therapy.

scholarly journals Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Zhiwei Jiang ◽  
Yue Xi ◽  
Kaichen Lai ◽  
Ying Wang ◽  
Huiming Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Sheet ◽  
Sheet Thickness ◽  
Collagen Type I ◽  
Type I ◽  
Cell Sheets ◽  
Cell Sheet Technology ◽  
Rat Bone

Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.

A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

1967 ◽  
Vol 25 ◽  
pp. 132-133
Author(s):  
R. Stephens ◽  
G. Schidlovsky ◽  
S. Kuzmic ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Sheet ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Electron Microscopic ◽  
Cell Sheets ◽  
Morphological Alterations ◽  
Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

Perspectives

2018 ◽  
Author(s):  
Michael Szollosy
Keyword(s):  
New Technologies ◽  
Human Relationships ◽  
Cultural Imagination ◽  
Personal Lives ◽  
The Public ◽  
Social Organizations ◽  
The Social ◽  
Human Augmentation ◽  
Public Imagination

This chapter introduces the “Perspectives” section of the Handbook of Living Machines offering an overview of the different contributions gathered here that consider how biomimetic and biohybrid systems will transform our personal lives and social organizations, and how we might respond to the challenges that these transformations will inevitably pose to our ‘posthuman’ worlds. The authors in this section see it as essential that those who aspire to create living machines engage with the public to confront misconceptions, deep anxieties, and unrealistic aspirations that presently dominate the cultural imagination, and to include potential users in questions of design and utility as new technologies are being developed. Human augmentation and enhancement are other important themes addressed, raising important questions about what it means fundamentally to be ‘human’. These questions and challenges are addressed through the lens of the social and personal impacts of new technologies on human selves, the public imagination, ethics, and human relationships.

scholarly journals Obstacles to Developing Net-Zero Energy (NZE) Homes in Greater Toronto Area

Buildings ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 95
Author(s):  
Ghazal Makvandia ◽  
Md. Safiuddin
Keyword(s):  
Financial Incentives ◽  
New Technologies ◽  
Public Awareness ◽  
Ghg Emissions ◽  
Primary Data ◽  
Zero Energy ◽  
The Public ◽  
Greater Toronto Area ◽  
Net Zero Energy ◽  
Net Zero

Efforts have been put in place to minimize the effects of construction activities and occupancy, but the problem of greenhouse gas (GHG) emissions continues to have detrimental effects on the environment. As an effort to reduce GHG emissions, particularly carbon emissions, countable commercial, industrial, institutional, and residential net-zero energy (NZE) buildings were built around the globe during the past few years, and they are still operating. But there exist many challenges and barriers for the construction of NZE buildings. This study identifies the obstacles to developing NZE buildings, with a focus on single-family homes, in the Greater Toronto Area (GTA). The study sought to identify the technical, organizational, and social challenges of constructing NZE buildings, realize the importance of the public awareness in making NZE homes, and provide recommendations on how to raise public knowledge. A qualitative approach was employed to collect the primary data through survey and interviews. The secondary data obtained from the literature review were also used to realize the benefits, challenges, and current situation of NZE buildings. Research results indicate that the construction of NZE buildings is faced with a myriad of challenges, including technical issues, the lack of governmental and institutional supports, and the lack of standardized measures. The public awareness of NZE homes has been found to be very low, thus limiting the uptake and adoption of the new technologies used in this type of homes. The present study also recommends that the government and the academic institutions should strive to support the NZE building technology through curriculum changes, technological uptake, and financial incentives to buyers and developers. The implementation of these recommendations may enhance the success and popularity of NZE homes in the GTA.

Abstract 020: Cell-sheet-based Bioengineered Human Cardiac Tissue Using Pluripotent Stem Cells For Heart Repair And Disease Models

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Katsuhisa Matsuura ◽  
Tatsuya Shimizu ◽  
Nobuhisa Hagiwara ◽  
Teruo Okano
Keyword(s):  
Cardiac Tissue ◽  
Subcutaneous Tissue ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Embryoid Bodies ◽  
Cardiac Differentiation ◽  
Cardiac Cell ◽  
Cell Sheets ◽  
High Efficient ◽  
Human Cardiac Tissue

We have developed an original scaffold-free tissue engineering approach, “cell sheet engineering”, and this technology has been already applied to regenerative medicine of various organs including heart. As the bioengineered three-dimensional cardiac tissue is expected to not only function for repairing the broad injured heart but also to be the practicable heart tissue models, we have developed the cell sheet-based perfusable bioengineered three-dimensional cardiac tissue. Recently we have also developed the unique suspension cultivation system for the high-efficient cardiac differentiation of human iPS cells. Fourteen-day culture with the serial treatments of suitable growth factors and a small compound in this stirring system with the suitable dissolved oxygen concentration produced robust embryoid bodies that showed the spontaneous beating and were mainly composed of cardiomyocytes (~80%). When these differentiated cells were cultured on temperature-responsive culture dishes after the enzymatic dissociation, the spontaneous and synchronous beating was observed accompanied with the intracellular calcium influx all over the area even after cell were detached from culture dishes as cell sheets by lowering the culture temperature. The cardiac cell sheets were mainly composed of cardiomyocytes (~80%) and partially mural cells (~20%). Furthermore, extracellular action potential propagation was observed between cell sheets when two cardiac cell sheets were partially overlaid, and this propagation was inhibited by the treatment with some anti-arrhythmic drugs. When the triple layered cardiac tissue was transplanted onto the subcutaneous tissue of nude rats, the spontaneous pulsation was observed over 2 months and engrafted cardiomyocytes were vascularized with the host tissue-derived endothelial cells. These findings suggest that cardiac cell sheets formed by hiPSC-derived cardiomyocytes might have sufficient properties for the creation of thickened cardiac tissue. Now we are developing the vascularized thickened human cardiac tissue by the repeated layering of cardiac cell sheets on the artificial vascular bed in vitro.

Abstract 8: Transplantation of Mouse Adipose Derived Stem Cell Sheet into Infarcted Rat Myocardium Increase Cell Engraftment and Cardiac Function

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Jong-Ho Kim ◽  
Hyung Joon Joo ◽  
Ha-Rim Seo ◽  
Long-Hui Cui ◽  
Mi-Na Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
Cell Sheet ◽  
The Other ◽  
Differentiation Potential ◽  
Significance Level ◽  
Infarct Region ◽  
Cell Engraftment ◽  
Cell Proliferation And Differentiation

Background: Cell sheet technology has magnified as an important transplantation skill. Mouse adipose derived stem cells (mADSCs) can secrete various growth factors, which promote the repair of damaged cardiomyocyte and protecting cells from death. In addition, autologous cell source to easily obtain from patients are promising candidates for cell therapy in cardiovascular field. Methods: mADSCs were confirmed stem cell properties and secreted cytokines were evaluated in vitro. Eighteen acute myocardial infarction (AMI) rats were divide into 3 group; sham (n=6), suspended mADSCs (n=6), and mADSCs sheet (n=6) groups. In the mADSCs sheet group, 60х106 cells were cultured for 2 days onto temperature-responsive polymers and the sheets were then transplanted over the infarct region. In additional, the sheet was made of carboxyfluorescein diacetate succinimidyl ester (CFDA) -labelled mADSCs to confirmed cell survival. Engraftment and differentiation were blindly assessed after 28 days. Results: The mADSCs expressed Sca-1+ and represented multi-differentiation potential. Interestingly, EGF and IGF levels significantly increased in the mADSCs sheet. Significant improvements in ejection fraction and fraction shortening value were observed in the mADSCs sheet and suspended mADSCs groups compared with the sham group at 14 and 28 days. But, it was not higher significance level in the mADSCs sheet group than in the suspended mADSCs group. Engraftment range and fibrosis area of infarct region were significantly higher in the mADSCs sheet group compared to the other two groups at 4, 14 and 28 days. In significant expressed cytokines (bFGF, IL-1a, IL-1ra, CT-1, EGF, TGFb1, IGF-1, IGF-2 and MCP-1) were observed in the mADSCs sheet group compared with the other 2 groups at 28 days after transplantation. In addition, in the mADSCs sheet was confirmed endothelial differentiation by Von Willebrand factor (vWF) at 4, 14 and 28 days. Conclusions: Transplantation of mADSCs sheet into rat infarcted myocardium increased engraftment and survival of transplanted cells. The mADSCs sheet is very useful for the study of stem cell proliferation and differentiation as well as for cell therapy in cardiovascular field.

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