scholarly journals Influence of Enzyme Concentration and Hydrolysis Time on Soluble Protein Content of Protein Hydrolysate Prepared from Apple Snail (Pila ampullacea)

2021 ◽  
Vol 2 (02) ◽  
pp. 26-29
Author(s):  
Andre Yusuf Trisna Putra ◽  
Dedin Finatsiyatull Rosida ◽  
Anugerah Dany Priyanto
Keyword(s):  
Enzymatic Hydrolysis ◽  
Protein Content ◽  
Soluble Protein ◽  
Protein Hydrolysate ◽  
Enzyme Concentration ◽  
Total Soluble Protein ◽  
Apple Snail ◽  
Soluble Protein Content ◽  
Hydrolysis Time ◽  
Enzyme Substrate

The objective of this study was to evaluate soluble protein content of protein hydrolysates obtained by enzymatic hydrolysis of apple snail using a trypsin enzyme. Apple snail were collected from traditional market at Pabean-Sidoarjo. Trypsin enzyme was used in enzymatic hydrolysis. The two variables, enzyme/substrate (E/S) ( 0.01, 0.05, 0.1) ratio and hydrolysis time (3 h, 6 h, 9 h, 12 h, 15 h, 18 h) and was used to produce the apple snail hydrolysate. The result showed that soluble protein content was about 2.3%-4.52%. The increase E/S ratio and hydrolysis time, the higher soluble protein content values was. The highest total soluble protein was achieved E/S 0.1 ratio at 12 h, 4.52%. But, after 12 h hydrolysis time, soluble protein was decreased. Optimum treatment to hydrolyzing apple snail using trypsin enzyme was E3H4 treated (E/S 0.1 ratio and 3 h)

scholarly journals Effect of Hydrolysis time and Papain Concentration on Some Properties of Apple Snail (Pilla ampullacea) Hydrolysate

2020 ◽  
Vol 1 (02) ◽  
pp. 1-5
Author(s):  
Andre Yusuf Trisna Putra ◽  
Dedin Finatsiyatull Rosida ◽  
Anugerah Dany Priyanto
Keyword(s):  
Soluble Protein ◽  
Enzyme Concentration ◽  
Total Soluble Protein ◽  
Apple Snail ◽  
Gut Content ◽  
Hydrolysis Time ◽  
Umami Taste ◽  
Nutritional Potential

content and total peptide content were investigated in the non-farmed freshwater apple snail (Pila ampullacea) to understand its nutritional potential as alternative umami taste enhancer. Apple Snail samples with removed gut content were collected from a local snail non-farm in Surabaya City. Papain enzyme was used. The two variables, hydrolysis time (3, 6, 9, 12, 15, 18 h) and enzyme concentration (1%, 5%, 10%), was used to produce the apple snail hydrolysate. The result showed that total soluble protein was about 2.1%-7.3%, whereas total peptide content was 5.05-15.73mg/ml. The highest total soluble protein was achieved at 12 hour. Longer hydrolysis time significantly effect on total peptide content of apple snail hydrolysate.

scholarly journals Protein pattern of canola (Brassica napus L.) changes in response to salt and salicylic acid in vitro

2015 ◽  
Vol 52 (1-2) ◽  
pp. 19-36 ◽  
Author(s):  
Roya Razavizadeh
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Protein Content ◽  
Soluble Protein ◽  
Interactive Effects ◽  
Total Soluble Protein ◽  
Protein Patterns ◽  
Soluble Protein Content ◽  
Brassica Napus L ◽  
Total Soluble Protein Content

Abstract The effect of salicylic acid (SA) on the salt (NaCl) tolerance mechanism was studied in canola plants (oilseed rape, Brassica napus L.) by molecular and physiological experiments in plant tissue culture. Seeds of B. napus ‘Ocapy’ were germinated at 0, 50, and 100 mM NaCl on Murashige and Skoog (MS) medium containing different levels (0, 2, and 5 μM) of SA for 4 weeks. Total chlorophyll, carotenoid, and flavonoid content increased in response to interactive effects of SA and NaCl treatments at some concentrations. Proline content was increased under salt and SA treatments in shoot and root tissues. Salt alone and in combination with SA increased the total soluble protein content of shoots only, while the different concentrations of SA in the culture media affected variously the total soluble protein content. Protein patterns of shoots and roots showed some remarkable differences, based on gel electrophoresis and the consequent analysis of bands by ImageJ program. The relative expression of 15 and 12 protein bands in shoots and roots, respectively, differed under the applied treatments. In addition, the protein profile indicated that salinity and SA regulate the expression of salt-stress-inducible proteins as well as induced de novo synthesis of specific polypeptides. The findings may help to explain the salt tolerance mechanisms and to produce salt-tolerant canola plants.

scholarly journals KARAKTERISASI HIDROLISAT PROTEIN KEDELAI HASIL HIDROLISIS MENGGUNAKAN PROTEASE DARI TANAMAN BIDURI (Calotropis gigantea)

2007 ◽  
Vol 13 (1) ◽  
pp. 7-13
Author(s):  
Yuli Witono ◽  
Aulanni’am Aulanni’am ◽  
Achmad Subagio ◽  
Simon Bambang Widjanarko
Keyword(s):  
Enzymatic Hydrolysis ◽  
Maillard Reaction ◽  
Soy Protein ◽  
Soluble Protein ◽  
Protein Hydrolysate ◽  
Soluble Protein Content ◽  
Organoleptic Evaluation ◽  
Umami Taste ◽  
Soy Protein Hydrolysate ◽  
Hydrolysis Of

Properties of soy protein hydrolysate produced by protease from biduri plant were studied. The soy protein hydrolysate had different properties due to various concentrations and hydrolysis times of protease from biduri. Enzymatic hydrolysis of the soy protein decreased significantly in the TBA value. This process increased the soluble protein content and promoted the Maillard reaction, resulting in a more brown color. Moreover, the soy protein hydrolysate had a higher value of umami taste by organoleptic evaluation.

scholarly journals Salt Stress and Homobrassinosteroid Interactions during Germination in Barley Roots

2014 ◽  
Vol 42 (2) ◽  
pp. 446-452 ◽  
Author(s):  
Sevgi MARAKLI ◽  
Aslihan TEMEL ◽  
Nermin GOZUKIRMIZI
Keyword(s):  
Superoxide Dismutase ◽  
Protein Content ◽  
Mitotic Activity ◽  
Enzyme Activities ◽  
Soluble Protein ◽  
Primary Root ◽  
Total Soluble Protein ◽  
Soluble Protein Content ◽  
Mitotic Abnormalities ◽  
Total Soluble Protein Content

Potential alleviation effects of Homobrassinosteroid (HBR) (0.5 and 1 µM HBR) on root germination, cell division and antioxidant system enzymes (superoxide dismutase and catalase) of barley (Hordeum vulgare L. cv. ‘Hilal’) roots grown under different salt concentrations (150 mM and 250 mM) were investigated during 48 and 72 h at dark with their controls. Salt applications decreased primary root lengths, seminal root lengths, number of roots from one seed, mitotic activity and induced mitotic abnormalities. In addition, salt application decreased protein content but increased enzyme activities both at 48 h and 72 h when compared to control. Roots treated with HBR enhanced root lengths and root number. HBR-treated roots showed more mitotic activity, mitotic abnormalities and significant enlargements at the root tips when compared to controls and only salt-treated samples. Salt + HBR applications stimulated root lengths and showed more mitotic activity and mitotic abnormalities when compared to only salt-treated samples. Salt application increased superoxide dismutase and catalase activities both at 48 h and 72 h.  However, HBR application decreased total soluble protein content and increased enzyme activities especially at 48 h compared to control. Depending on concentration and timing, salt + HBR treatments showed varying results in total soluble protein content and enzyme activities. There was also a significant change in protein banding patterns of experimental groups separated by SDS-PAGE. These findings are expected to contribute to understanding of how HBR affect barley roots grown under different salt concentrations.

scholarly journals Effects of Water Particles in the Jinjiang River Estuary on the Physiological and Biochemical Characteristics of Microcystit Flos-Aquae

2021 ◽  
Author(s):  
Yiting Nan ◽  
Peiyong Guo ◽  
Hui Xing ◽  
Sijia Chen ◽  
Bo Hu ◽  
...  
Keyword(s):  
Particle Size ◽  
Particulate Matter ◽  
Protein Content ◽  
Suspended Particulate Matter ◽  
Soluble Protein ◽  
Suspended Particles ◽  
Dose Effect ◽  
Soluble Protein Content ◽  
Sod Activity ◽  
Suspended Particulate

Abstract The effects of different concentrations (100,150,200,250 mg/L) and different particle sizes (0–75µm, 75–120µm, 120–150µm, 150–500µm) on soluble protein content, SOD and CAT activity, MDA content, chlorophyll a content and photosynthetic parameters of Microcystis flos-aquae were studied, the mechanism of the effect of suspended particulate matter on the physiology and biochemistry of Microcystis flos-aquae was discussed. The results showed that the soluble protein content of Microcystis flos-aquae did not change obviously after being stressed by suspended particles of different concentration/diameter. The SOD activity of Microcystis flos-aquae increased at first and then decreased with the increase of the concentration of suspended particulate matter. The SOD activity of Microcystis flos-aquae reached 28.03 U/mL when the concentration of suspended particulate matter was 100 mg/L. The CAT activity of Microcystis flos-aquae increased with the increase of the concentration of suspended particles, and reached the maximum value of 12.45 U/mgprot in the concentration group of 250 mg/L, showing a certain dose-effect. The effect of small particle size on SOD, CAT and MDA of Microcystis flos-aquae was more significant than that of large particle size. The larger the concentration and the smaller the particle size, the stronger the attenuation of light and the lower the content of chlorophyll a. Both Fv/Fm and Fv/F0 of Microcystis flos-aquae increased at first and then decreased under different concentration/size of suspended particles. The relative electron transfer rate gradually returned to the normal level with the passage of time. There was no significant difference in α value between treatment group and control group, ETRmax and Ik decreased.

scholarly journals Mutation in neurospora crassa with leaf extract of citrus aurantifolia and their soluble protein content

1970 ◽  
Vol 19 (2) ◽  
pp. 151-155
Author(s):  
Apurba Lal Ray ◽  
Mahbuba Akhter Jahan ◽  
Tahsina Rahim
Keyword(s):  
Neurospora Crassa ◽  
Protein Content ◽  
Soluble Protein ◽  
Mycelial Growth ◽  
Leaf Extract ◽  
Mutagenic Effect ◽  
Wild Type ◽  
Soluble Protein Content ◽  
Citrus Aurantifolia ◽  
Morphological Mutants

Leaf extract of Citrus aurantifolia exhibited remarkable inhibitor effect on the radial mycelial growth of Neurospora crassa. The extract also showed mutagenic effect and atleast six morphological mutants of the fungus were detected including albino (al 243), vigorous (vg 117), fluffy (fl 220), colonial (cl 232), conidial band (con. band 171) and dirty (dir 83). The mutants were used for estimation of soluble protein in comparison with the wild type (Ema). The soluble protein content increased to some extent in case of the mutants con. band 171 (192.86 μg/ml), cl 232 (188.57 μg/ml) and vg 117 (186.43 μg/ml) as compared to the wild type (182.14 μg/ml). On the other hand, the soluble protein content was remarkably decreased in case of the mutant al 243 (94.28 μg/ml), which was about 50% less than the control. This indicates that the leaf extract not only effect colony morphology but possesses profound effect on growth and metabolism of the fungus. Key words: Neurospora crassa; Mutation; Leaf extract; Soluble protein DOI: http://dx.doi.org/10.3329/dujbs.v19i2.8958 DUJBS 2010; 19(2): 151-155

scholarly journals Mycorrhizal efficacy of trifoliate orange seedlings on alleviating temperature stress

2011 ◽  
Vol 57 (No. 10) ◽  
pp. 459-464 ◽  
Author(s):  
Q.S. Wu
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Protein Content ◽  
Temperature Stress ◽  
Soluble Protein ◽  
Mycorrhizal Colonization ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Soluble Protein Content ◽  
Entry Points

Citrus plants often suffer from temperature stress, which seriously inhibits tree growth and even results in tree death. The present experiment was conducted to evaluate the effects of Glomus mosseae on growth, root morphology, superoxide dismutase (SOD) and catalase (CAT) activities, and soluble protein content of trifoliate orange (Poncirus trifoliata) seedlings at low (15°C), optimum (25°C) and high (35°C) temperatures. Sixty-eight days after temperature stresses, mycorrhizal colonization and number of both entry points and vesicles were significantly inhibited by low or high temperature. Mycorrhizal seedlings recorded significantly higher growth characteristics than non-mycorrhizal seedlings at both optimum and high temperatures, but the beneficial effects were almost lost at low temperature. Generally, mycorrhizal seedlings presented notably higher root traits (projected area, surface area, number of forks and volume) than non-mycorrhizal seedlings regardless of temperature levels. Mycorrhizal colonization significantly increased SOD and CAT activities and soluble protein content at high temperature, increased only SOD activity at optimum temperature, and decreased only soluble protein content at low temperature. It suggests that mycorrhizal alleviation of temperature stress in trifoliate orange seedlings was at high temperature, but the alleviation was obviously weakened at low temperature.

Ozone effects on soluble protein content of Acer negundo, Quercus robur and Platanus spp. pollen

Aerobiologia ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 443-447 ◽  
Author(s):  
H. Ribeiro ◽  
L. Duque ◽  
R. Sousa ◽  
I. Abreu
Keyword(s):  
Protein Content ◽  
Soluble Protein ◽  
Quercus Robur ◽  
Soluble Protein Content ◽  
Acer Negundo
Sign in / Sign up

Export Citation Format

Share Document